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Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison


Micromorphology of Schizophyllum commune attack in pine (Pinus sylvestris) wood
1983 - IRG/WP 1184
The decay pattern caused by Schizophyllum commune in pine latewood tracheids was studied using both light and transmission electron microscopy. The attack began as isolated concentric slits within the S2 layer with extensive lamellation and separation of individual wall layers observed in subsequent stages of decay. The slits resulting from attack appeared to be formed in thin, regular concentric ...
T Nilsson, G F Daniel


Natural durability of Norwegian wood species for above ground applications – Project presentation and preliminary results
2006 - IRG/WP 06-10594
In Norway exterior wood structures have traditionally nearly exclusively been made of treated and untreated Norway spruce (Picea abies (L.) Karst) and Scots pine (Pinus sylvestris L.). In recent years there has been a tendency that other tree species, like various domestic hardwoods and imported species have been used in exterior above ground applications, often unfinished. For several wood speci...
P O Flæte, G Alfredsen, F G Evans


Respiration measurement of dry-rot
1995 - IRG/WP 95-10095
Methods for measuring the production of carbondioxide from wood infected with the dry-rot fungus were evaluated. By enclosing whole wood blocks and measuring the concentration of gas in the headspace by gas chromatography, an exponential increase in the concentration of CO2 was observed for at least 2 months. This technique could therefore present a method for evaluating various treatments of dry-...
L Toft


Water-Borne Copper Naphthenate: An Emerging Wood Preservative
2002 - IRG/WP 02-30280
Waterborne copper naphthenate (WB Cu-N) was used to treat southern yellow pine (Pinus spp) and red maple (Acer rubrum) stakes. The treated stakes were exposed in test sites located in Florida, Michigan and Mississippi for 3 to 6 years. Copper Chromium Arsenate (CCA), Ammoniacal Copper Quat (ACQ) and Oil-borne Copper Naphthenate (OB Cu-N) were also used to treat some stakes for comparison. Results...
P Nzokou, D P Kamdem, M H Freeman


On the biotope of dry rot (Serpula lacrymans) in the wild
1995 - IRG/WP 95-10123
The True Dry rot fungus was found in the Himalayas several times in this century. The finds were able to cross breed with Serpula lacrymans from Denmark and therefore all belong to this species (Harmsen 1960). In order to understand its successful colonisation in buildings we were interested in studying the natural biotope, i.e. the moisture, temperature and soil relationships. All together 15 fru...
J Bech-Andersen, S A Elborne, K Bech-Andersen


Assessment of treated wood leachates genotoxicity with a bacterial test
1997 - IRG/WP 97-50089
Genotoxicity is known as the damage caused by environmental stressors (biological, chemical and physical) on the genetic material of an organism. This toxicological effect can be assessed by a lot of biological assays and especially by bacterial tests. These tests are frequently performed on environmental samples or on pures substances and are in that case, strongly correlated to the carcinogenic ...
P Marchal, C Martin


Comparison between two laboratory test methods for determining the effectiveness on wood preservatives against blue stain in fresh wood
1987 - IRG/WP 2289
Most of the work done on determination of the effectiveness of new formulae for treating fresh wood against blue stain have been focussed on their use in the manufacture of saw timber. This work explains two laboratory methods, one which simulates the working and climatological conditions of factories making packages for fruit and vegetables in the Spanish Levante, showing that contamination of wo...
A M Navarrete, M T De Troya


The quantitative determination of quaternary ammonium compounds in treated timber – results of an extended ring test
2002 - IRG/WP 02-20240
The determination of quaternary ammonium compounds (QAC) by means of a 2-phase-titration or photometry is a well established procedure for the quantification of cationic detergents in aqueous solutions. It is known that this analytical principle can also be used for the measurement of QAC extracted from treated timber. However only few information are available concerning the reproducibility or th...
E Melcher, C Bornkessel, J Gunschera, R Hamberg, H Härtner, H-N Marx, U Schoknecht, J Wittenzellner


Natural durability transfer from sawmill residues of white cypress (Callitris glaucophylla). - Part 4: Analysis of extracts and treated wood for active components
2000 - IRG/WP 00-20215
In order to facilitate the commercial implementation of a large project aimed at recovering 'waste' durability components from sawmill residues of Callitris glaucophylla, it has been essential to develop analytical methodology for the important bioactive components of the heartwood extract. This methodology will be used 1) to standardise the activity of successive production batc...
Hui Jiang, M J Kennedy, L M Stephens


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


Relative tolerance of CCA by larvae and adults of the common shipworm, Bankia gouldi
1992 - IRG/WP 92-4174
Southern pine sapwood "2 x 4´s" were created by bolting together four panels 13 mm thick by 89 mm wide by 457 mm long. The two outer laminates were untreated and the two inner laminates were each treated to different CCA retentions, ranging from 8.0 to 43.2 kg/m³. Test specimens were exposed from May to October in a tributary of the Chesapeake Bay. Larvae were unable to settle on and burrow into...
B R Johnson


Subterranean termite attack potential in field test sites: assessment methods and field characterization
2003 - IRG/WP 03-10472
To assess subterranean termite attack potential in the field and seasonal variations, trials were set up in each of two managed silvicultural plantations, of Eucalyptus globulus and Pinus pinaster, respectively. Termite occupancy in lying dead wood was evaluated by an adaptation of the line-intersection method, and attack potential in soil was assessed by a system of stakes inserted into the groun...
T Nobre, L Nunes, L Brinca, D E Bignell


Sapstain fungi associated with soft wood species in Kerala, India
1998 - IRG/WP 98-10260
Most of the packing case, plywood and match industries distributed throughout Kerala State, India utilise different timber species. The major problem in the utilisation of these species is their susceptibility to fungal sapstain and mould growth due to the conducive climatic conditions in the State. Several wood-based industries were surveyed and based on the economic importance and extent of saps...
E J M Florence, J K Sharma, R Gnanaharan


Collaborative field trial out-of-ground contact
1982 - IRG/WP 2179
At the 12th meeting of the IRG in Sarajevo, Yugoslavia it was decided after considerable discussion that the best way to proceed with the work of the subgroup was to centre it around a co-operative field trial based on the L-joint test detailed in IRG/WP/2157. At the same time it was agreed that interested laboratories would also conduct their own methods, particularly the German planner test and ...
D J Dickinson, A F Bravery, J K Carey


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


Test methods for wood preservatives against Lyctus: (1) Testing of treated veneer. (2) Testing of glue-line treated plywood. (Laboratory methods)
1977 - IRG/WP 293
Powder-post beetles destroy large quantities of veneer and plywood. Two test procedures are described which can be used to support application-oriented research into veneer and plywood preservatives. These procedures simulate practical conditions on a laboratory scale. Their characteristic feature is that wood species of special susceptibility are used for the tests; the susceptibility of the spec...
S Cymorek


The biological effectiveness of wood modified with heptadecenylsuccinic anhydride against two brown rot fungi: Coniophora puteana and Gloeophyllum trabeum
1992 - IRG/WP 92-3705
A modified soil block test was carried out using wood samples reacted with heptadecenylsuccinic anhydride (ASA). This modification gave good resistance to decay brought about by the brown rot fungi Coniophora puteana and Gloeophyllum trabeum during the twelve week exposure period. Results indicated that there was a good correlation between increased loading of modifying reagent and an increase in ...
C Codd, W B Banks, J A Cornfield, G R Williams


Water-borne DNBP wood preservatives - Preparation and performance
1987 - IRG/WP 3407
Three waterborne wide-spectrum ground-contact DNBP (2-sec-butyl-4,6-dinitrophenol) wood preservative formulations and their performance are presented. Two are double-treatment formulations in which insolubilization and precipitation of the preservative is attained by salt formation or by formation of a water insoluble Cu/NH3/DNBP complex of formula [DNBP]2 [Cu(NH3)2]2 the clarified structure of wh...
W E Conradie, A Pizzi


Utilization of coconut timber from north Sulawesi, Indonesia. Part 1: Durability
1994 - IRG/WP 94-30044
Coconut timber from non-productive plantations is a byproduct of an agricultural crop and, by the same token, constitutes a renewable resource which may serve as a complement to or, at least in part, as a substitute for traditional timbers in local markets. Its more extensive utilization is expected to contribute to the conservation of tropical rain forests. Export of coconut timber and/or wood pr...
R-D Peek


Aboveground Microbial Decay Test of Biocide Treated and Untreated Wood Exposed to Danish and Humid Tropical Climates
2004 - IRG/WP 04-20306
A co-operative study was initiated to compare the aboveground field decay test of untreated and preservative-treated timbers under Nordic (Danish) and a representative humid tropical (Malaysian) environment according to CEN standard method. Matched specimens of untreated and both preservative-impregnated and surface-treated wood were simultaneously exposed at The Danish Technological Institute (DT...
A H H Wong, N Morsing, K H Henriksen, S Ujang


IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water. Progress Report 3: Report of first inspection of samples at Lae, Papua New Guinea
1978 - IRG/WP 446
Samples for the international marine test were placed on frames numbered I to VII. Frames numbered VII of both CCA and CCB trials are being stored dry in the laboratory at F.P.R. & D.C. Frames I to VI of both trials were suspended in the sea at Lae Harbour (Morobe Province, Papua New Guinea) on 6th December 1977. The first inspection of the CCA trial was carried out on 8th June 1978, at which ...
S M Cragg, C R Levy


Interim balance after 20 months of lap-joint exposure
1999 - IRG/WP 99-20164
The application of natural resins and integrated resin systems to improve the performance of Pinus sylvestris was assessed in a lap-joint test according to DIN V ENV 12037 (1996). Lap-joints of Pinus sapwood were exposed at the test field in Hamburg (Germany) in May 1997. The treated lap-joints were assessed with regard to the performance of the resin treatments out of ground. The exposure of lap-...
M Sailer, A O Rapp, R-D Peek, A J Nurmi, E P J Beckers


Extracellular substance from the white rot basidiomycete Irpex lacteus involved in wood degradation
1992 - IRG/WP 92-1571
Cellulases, phenol oxidase, and a substance that both produced and reduced H2O2 were isolated from cultures containing wood or glucose on which the white-rot fungus Irpex lacteus was growing. The rate of wood degradation by the fungus with different amounts of glucose in the medium was measured. More of the substance that produced and reduced H2O2 was found extracellularly in cultures containing w...
H Tanaka, T Hirano, G Fuse, A Enoki


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