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The invasion channels of damage fungi in bamboo lumber
2010 - IRG/WP 10-10712
The micro-agents on bamboo lumber damage are mould, stain, and decay fungi. This research is about invasion channels of bamboo lumber damage fungi by scanning electron microscope (SEM). The results showed that the conidia of mould were only accumulated on the surface of bamboo and no hyphae detected in bamboo vascular bundle, and hyphae of stain penetrated into bamboo and grew in metaxylem vessel...
Xingxia Ma, Mingliang Jiang, Daochun Qin


A genomic sequencing approach to study wood decay and copper tolerance in the brown rot fungus, Antrodia radiculosa
2010 - IRG/WP 10-10720
We used Illumina paired-end short read sequencing (76 nt, 300 bp insert size) to produce a de novo assembly of the genome of Antrodia radiculosa, a copper-tolerant brown rot fungus that is capable of aggressive wood decay. Quality analysis of the base calls in the dataset (8.95 Gb) showed that the majority of the nucleotide sequence was of the highest quality with 5% in the lowest quality group. ...
J D Tang, T Sonstegard, S Burgess, S V Diehl


Early detection of colonisation by blue stain and mould on modified wood using PCR technique
2010 - IRG/WP 10-10730
The aim of this research was the early detection of fungal attack on the surfaces of modified and unmodified wood after natural weathering. The investigation was carried out by molecular techniques and the development and establishment of genus/species specific PCR for important moulds and sapstain fungi was part of this work. After adaption of the method, a monitoring of the succession of Ascomyc...
A Steitz, B Schmöllerl, N Pfabigan, S Bollmus, G Grüll


Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze


Degradation of Carbon-Based Preservatives by Black-stain Fungi
2010 - IRG/WP 10-30533
Some black-stain fungi are known to exhibit resistance to carbon-based preservatives. However, the cause of this resistance is unclear. The fungi may have mechanisms to tolerate these chemicals, or they may be able to degrade and detoxify them. Fungi capable of degrading carbon-based preservatives are of interest for possible bioremediation of carbon-based preservative-treated wood at the end of i...
R Stirling, P I Morris


Gene expression analysis of a copper-tolerant brown rot fungus on MCQ-treated wood
2011 - IRG/WP 11-10748
Most brown rot fungi are copper-tolerant, which makes them difficult to control with copper-based wood preservatives like MCQ. To better understand what biological processes are regulated, we used our model species, Antrodia radiculosa, to examine expression of genes on MCQ-treated wood. Our hypothesis was genes that decreased copper bioavailability would be up-regulated early, when wood showed ...
J D Tang, A Perkins, S V Diehl


The durability of acetylated radiata pine sapwood (Accoya). Results from five years testing
2011 - IRG/WP 11-30560
New Zealand grown radiata pine sapwood was modified using the Accsys PLC acetylation process (Accoya). Following treatment, various test samples were exposed in the Scion Accelerated Field Test facility (Fungus Cellar) and in exterior ground contact field tests. In the Fungus Cellar, performance was compared with that of radiata pine treated with CCA to Hazard Classes H3.2 (0.37% m/m Cu+Cr+As) and...
M Hedley, D Page, J van der Waals, K Nasheri, G Durbin


Effects of microclimate, wood temperature and surface colour on fungal disfigurement on wooden claddings
2012 - IRG/WP 12-20490
Wooden claddings are common in façades in Norway, and Norway spruce (Picea abies) is the most frequently used species. The cladding is a major part of the facade, and it has visual requirements that may define the aesthetic service life. The visual changes that occur during weathering can be colour changes, abrasion or wear, blistering, flaking, and even cracks in the wood or coating, but more of...
L Ross Gobakken, G I Vestøl


Tannin resin-boron associations: Leaching and biological resistance
2012 - IRG/WP 12-30587
The easy leaching of boron in wood preservation formulations has allowed to use this fungicide only for short term applications. The recently discovered adduct with flavonoids allows boron to resist longer periods of time within wood and consequently extend its life. Two different leaching treatments were compared and the fungal and termite decay was examined. The biological tests have shown extre...
G Tondi, S Wieland, N Lemenager, A Petutschnigg, A Pizzi, M-F Thevenon


Field-testing of Norway Spruce Claddings with Monitoring of Moisture Content, Material Temperature and Microclimate
2012 - IRG/WP 12-20502
The physical function of a cladding is to protect the interior construction. Under normal circumstances the performance requirements can be met for a very long time, meaning that the technical service life of a wooden cladding can be very long. Since the cladding is a major part of the facade, it also has visual requirements that may define the aesthetic service life, and often it is much shorter ...
G I Vestøl, L Ross Gobakken


Influence of a pulsed electric field on the growth of surface fungi
2013 - IRG/WP 13-10795
The colonization of mold and staining fungi are not only an aesthetical problem. Blue stain fungi are able to penetrate paint films. Maintenance of coatings on wood are time consuming and costly. A new approach uses a pulsed electric field (PLEOT) in order to protect wood against surface fungi. This technology is evaluated in non-sterile laboratory conditions on different coated and uncoated Scots...
M Starck, A Treu


Detection of Aureobasidium as the dominant fungus on coated wood
2013 - IRG/WP 13-10796
Wood is often covered with a coating in order to protect the surface. Unwanted dark mold growth on paint covered surfaces in external conditions is a familiar phenomenon. Simple microbial techniques have commonly been used to determine the dominant fungal species present in the discoloured area, for example morphological analysis of microscopic preparations of fungal pieces sticking to an adhesive...
E J Van Nieuwenhuijzen, M F Sailer, R A Samson, O C G Adan


Image analysis for mould and sapstain detection on wood
2013 - IRG/WP 13-20519
Laboratory tests for mould growth on wood products are time consuming and rely on visual assessments of specimens utilizing subjective rating systems. Accelerated laboratory methods are needed that can provide rapid, quantitative assessment of mould and sapstain growth on solid and composite wood products. Image analysis of scanned spore imprints from southern pine or oriented strandboard (OSB) sp...
C A Clausen, V W Yang


The activity of a wood-decaying fungus during drying and rewetting cycles measured by isothermal calorimetry
2013 - IRG/WP 13-20526
Wood decaying fungi are an essential part of any ecosystems as they are the main decomposers of cellulose and lignin-containing materials. But as wood is a common building material the risk for decay fungi growth and subsequent degradation of our construction material is a concern. There are important physiological aspects of the growth and activity of decay fungi that are unknown today, and witho...
S Johansson, L Wadsö, A Pilgård, G Alfredsen


Effect of Nano-silver and Nano-copper and Nano-zinc oxide on Paulownia wood exposed to white-rot fungus
2013 - IRG/WP 13-30635
Paulownia is known as a fast growing tree and it can be used in several applications. The present study investigated the effect of Nano-silver and Nano-copper and Nano- zinc oxide on resistance of Paulowina (Paulownia fortunei) wood against the white rot fungus (Coriolus versicolor). Wood specimens with a dry density of 0.37g/cm3 were impregnated with a 400 ppm aqueous suspension of micronized na...
M Akhtari, M Ganjipour


Using hyperspectral imaging to detect and quantify mould and blue stain on coated and uncoated wood
2014 - IRG/WP 14-10822
Hyperspectral imaging has been applied on the surface of samples of coated and uncoated Norway spruce (Picea abies) as a new technique to detect and quantify growth of mould and blue stain fungi. Principal Component Analysis of the hyperspectral images resulted in a clear visualization of the fungi and the amount of mould coverage could be estimated. This indicates that NIR hyperspectral imaging c...
L Ross Gobakken, I Burud, A Flø, K Kvaal, T K Thiis


Molecular characterization and biodiversity of wood-decaying fungi in French Guiana
2014 - IRG/WP 14-10825
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests and irregular occurrence carpophores make it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are better known. The purpose of this work was to enhance classification by integrating new data that...
A Zaremski, L Gastonguay, C Zaremski, J Beauchene


Protein extraction from wood decay fungus Postia placenta
2014 - IRG/WP 14-10827
Wood decay fungi (often distinguished as white rot and brown rot) belonging to the basidiomycetes, are common inhabitants of forest litter, where they play an important role in carbon cycling. Brown rot fungi are perhaps the most important organism involved in the degradation of wood products, and of considerable economic importance. Brown rotted wood loses strength very early because of the rapid...
A Pilgård, P Arnold, K Richter


Reducing Extractives Stain in Western Red Cedar Sidewall Shingles
2014 - IRG/WP 14-30654
One of the aesthetic challenges that western red cedar shingles face is extractives redistribution stain, particularly in unpainted sidewall applications. Dip and pressure treatments with a mixture of quaternary ammonium compounds and alkyl amine oxides were investigated for their ability to prevent this stain. After nine months of exposure in Vancouver, extractives stain was present on nearly all...
R Stirling


The role of hydrophobins in surface growth by the Blue Stain fungi Aureobasidium pullulans
2015 - IRG/WP 15-10837
Hydrophobins are small proteins found exclusively in filamentous fungi. These proteins are able to assemble spontaneously into amphiphilic monolayers at hydrophobic–hydrophilic interfaces. Hydrophobins have a diverse role, e.g. allowing the fungi to break through interfaces during aerial hyphae formation, sporulation, fruit body production and cell penetration. In this ongoing study, we are look...
J Stenbæk, L Riber, J Blæsbjerg Nielsen, C Møller Hansen, B Jensen


The whitening cause of Korean wooden heritage by flame retardant treatment
2015 - IRG/WP 15-20560
Korean wooden heritage used to treat by flame retardants in order to protect fire. There are 2 types flame retardant using for wooden heritage. These flame retardants treatment are basic measure that apply to wooden heritage along with other fire protection. The flame retardants cause white stains by reacting with Korean traditional wood painting (Dancheong) and increase wood humidity. The Korean ...
Jin Qyu Kim, Gyu-Seong Han, Yong Jae Chung, Hwa Soo Lee, Dong Won Son


Interaction between Aspergillus niger fungus and other filamentous fungi on salt-agar medium and their growth on natural wood and wood treated with fungicide
2015 - IRG/WP 15-20569
Aspergillus niger (An) is very often used alone or in mixture with other filamentous fungi of the genus in testing biocides in disinfection procedures, safety, hygiene, medical fields as well as for testing resistance of wood and wood-based materials to moulds. Softwood species as well as hardwood are overgrown with An fungus very easily, causing drop of mainly the aesthetic value of wood product....
A Fojutowski, A Kropacz, A Koziróg


Mould growth on spruce claddings and the effect of selected influencing factors after 4 years of outdoor testing
2015 - IRG/WP 15-30671
Norway spruce (Picea abies) is the most frequently chosen wood species as claddings in façades in Norway, and is used both coated and uncoated. The visual changes that occur during weathering can be colour changes, abrasion or wear, blistering, flaking, and even cracks in the wood or coating, but more often growth of mould and blue stain fungi is the main challenge. A field test with synchronous monitoring of relative humidity, air temperature, material temperature and wood moisture content in Norway spruce claddings has been performed in southern Norway as part of the ClimateLife project. Visual evaluation of blue stain and mould growth according to EN 927-3 was performed, and evaluation data after 46 months exposure is presented. The objectives were to study the effect of 1) environment, 2) cardinal direction and 3) colour of the coated surface on growth of blue stain and mould fungi and to study the variation in relative humidity, air temperature, wood moisture content and material temperature. After 46 months exposure, the red coating system had generally the lowest mould ratings and the uncoated claddings had the highest. Of the white coloured claddings the ones facing south had lower mould ratings than those facing north, but the opposite were found for red coloured claddings. The relative humidity was higher in front of claddings exposed northwards and in a shaded environment compared to an open environment against south. In an open environment red coloured claddings had the lowest wood moisture content and highest material temperature, and red coloured claddings against south had the highest maximum material temperature.
L Ross Gobakken, G I Vestøl


Susceptibility of wood substrates to Aurebasidium pullulans at different temperatures
2016 - IRG/WP 16-10863
There is an increasing awareness of how the aesthetical performance of wood exposed outdoors changes over time and especially in the first few years after installation. Mould and blue stain fungi are biological agents that contribute to the weather grey colour on a wooden façade, and the blue stain fungi Aureobasidium pullulans is commonly identified as colonizer on coated and uncoated wood expos...
L Ross Gobakken, G Alfredsen


Identification of the key factors influencing development of the blue stain fungi
2016 - IRG/WP 16-10872
Service life of wood is more and more frequently affected by aesthetic requirements than functional ones. In order to elucidate various aspects of service life, model house with façade elements made of 22 materials was built on the field test site at Department of Wood Science and Technology in Ljubljana. Colour and fungal disfigurement is among other parameters monitored on this house. In parall...
D Kržišnik, B Lesar, N Thaler, M Humar


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