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Enhancing Our Understanding of Brown Rot Mechanisms through Catalytic Pretreatment and Cellulase Cocktail
2018 - IRG/WP 18-10909
A catalytic mechanism, described as the “chelator-mediated Fenton” (CMF) mechanism, is proposed to mimic the non-enzymatic action of brown rot fungi. A CMF treatment was used together with an enzymatic cocktail to study how wood was deconstructed and solubilized. This was done in-part to determine if the treatment mimicked the action of brown rot fungi, but also to explore improved treatment p...
S Tabor, L Orjuela, D Contreras, G Alfredsen, J Jellison, S Renneckar, B Goodell


Preliminary Investigation into the Natural Decay Resistance of Nigerian Grown Hevea brasiliensis and Mitragyna ciliata wood to Phanerochaete chrysosporium White-Rot Fungus
2018 - IRG/WP 18-10919
Wood is an important and versatile structural building material that finds applications in numerous uses. However, wood is also a biological material vulnerable to degradation by microbial activities; this is especially true in the tropics. Following the scarcity of highly durable species from our natural forests and the introduction of so many lesser used/durable wood species into the booming tim...
J M Owoyemi, U O Emmanuel


FTIR-ATR monitoring of chemical changes of thermo-chemically modified beech wood degraded by brown-rot fungus
2018 - IRG/WP 18-40823
Wood chemical modification with lactic acid oligomers (OLA) has been reported to confer promising properties for wood outdoors applications. To better understand the lactic acid interaction with wood, chemical changes following biological degradation have been characterized with a battery of tests. Fourier transform infrared (FTIR) spectroscopy has been proved to be a valuable tool for studying fu...
C Grosse, M Noël, M-F Thevenon, P Gérardin


Recent findings clarifying the mechanism of brown-rot decay protection in acetylated wood
2018 - IRG/WP 18-40827
This paper summarizes the findings obtained during my PhD research over the past three years. The results provide insights which help to understand the enhanced brown-rot decay resistance of acetylated wood with particular emphasis on the role of water. The protection acetylation provides is derived from moisture exclusion within the acetylated cell wall, but the exact mechanism by which water is ...
G Beck


The biodegradation of creosote oil by various species of white rot fungi and bacteria isolated from the contaminated soil
2018 - IRG/WP 18-50334
The aim of the study was to determine biodegradation effectiveness of the selected polycyclic aromatic hydrocarbons (PAHs) contained in creosote oil, by two white rot fungal species (Bjerkandera adusta and Irpex lacteus) and three bacterial species (Pseudomonas putida, Pseudomonas sp. OS4 and Rahnella aquatilis). Bacteria were isolated from the soil contaminated with petroleum hydrocarbons from th...
M Sydow, J Zabielska-Matejuk, E Kaczorek, AStangierska, A Kropacz


Non-stochiometric oxidation and ROS generation promoted by guaiacol lignin structures and lignocelluose surfaces may be a component of brown rot fungal degradation mechanisms
2019 - IRG/WP 19-10937
Model guaiacol compounds representing lignin monomers, as well as DHP-lignin and wood flour of controlled particle size were used to assess iron reduction at the pH of the natural wood cell wall. All compounds functioned as electron donors for ferric iron, with the lignin monomers demonstrating capacity for non-stochiometric reduction of iron with multiple moles of ferric ion reduced per mole of l...
Y Tamarua, M Yoshidaa, L D Eltisb, B Goodell


From fungal detoxification systems to wood durability in neotropical forests
2019 - IRG/WP 19-10946
White-rot fungi are able to mineralize all the wood components due in particular to an efficient detoxification system. We hypothesised that components of this detoxification systems, glutathione transferases, could be used as tools to explore the natural durability of neotropical wood species. Analysis of the interactions between six glutathione transferases of Trametes versicolor and extracts of...
T Perrot, G Salzet, N Amusant, J Duchene, E Gelhaye


Characterization of Pectinases from Brown-rot Fungus Fomitopsis palustris
2019 - IRG/WP 19-10951
Brown-rot fungi occur on softwood used as building materials and cause destructive breakdown of wood structure. Therefore, a more accurate understanding is important from the perspective of wood protection. Previous studies have reported that hyphae of brown-rot fungi go through bordered pits on tracheids when the fungi grow into softwood [Francis W.M.R.Schwarze (2007)], and torus existing in the ...
Y Tanaka, N Konno, T Suzuki, N Habu


Hydroxyl accessibility - A brief review of deuterium exchange studies on modified wood
2019 - IRG/WP 19-20656
Wood modification imparts dimensional stability and decay resistance. The mechanism(s) by which this occurs are thought to be related to the bulking of the wood cell wall and subsequent moisture exclusion. Hence, quantification of accessible hydroxyl (OH) groups is of great interest when evaluating and increasing the understanding of the performance of modified wood. The aim of this study was to b...
G Beck, C Hill, G Alfredsen


Biocidic effect of nano metal fluorides against brown-rot fungi
2019 - IRG/WP 19-30732
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredients in wood preservatives such as copper and boron. The effectiveness of nano metal fluorides was compared to sodium fluoride treated wood samples. Wood specimens treated with homo-dispersed sols of MgF2 and CaF2 were tested against brown-rot...
S M Usmani, K Klutzny, I Stephan, E Kemnitz


Relative efficacy of various oxine copper formulations against brown-rot fungi
2019 - IRG/WP 19-30741
This paper investigates the relative resistance of three different oxine copper formulations (oil-borne, water-borne, water-borne with pH-adjustment) as a preservative treatment for wood against brown-rot fungi. Impregnated southern pine sapwood cubes were exposed to R. placenta and G. trabeum cultures in a soil-block test. After eight weeks, the weight losses of the cubes were examined in relatio...
M Petruch, J Lloyd, A Taylor


Different levels of acetylation lead to groupwise upregulation of non-enzymatic wood degradation genes of Rhodonia placenta during initial brown-rot decay
2020 - IRG/WP 20-10958
Rhodonia placenta, often used as a model fungus to represent brown rot fungi, uses a two-stepped degradation mechanism to degrade wood. Regarding the overcoming of wood protection systems the initial degradation phase seems to be the crucial point. A new laboratory test enables the separation of the non-enzymatic oxidative and the enzymatic degradation phases, which has previously been proven chal...
M Kölle, R Ringman, A Pilgård


FTIR analysis of wood blocks decayed by brown-rot fungi
2020 - IRG/WP 20-10959
Calibration curves of the relative lignin contents in Cryptomeria japonica decayed by brown-rot fungi were developed with Infrared Spectroscopy and Klason technique. First, wood decay test was conducted using brown-rot fungi (Fomitopsis palusris, F. pinicola, Wolfiporia cocos, Gloeophyllum trabeum, and Neolentinus suffrutescens) and white-rot fungus (Trametes versicolor) was used as a comparison. ...
R Kondo, Y Horikawa, R Kose, M Yoshida


The iron reduction by chemical components of wood blocks decayed by wood rotting fungi
2021 - IRG/WP 21-10979
Brown-rot fungi, a group of wood rotting fungi, is well known to be one of major microorganisms that cause the deterioration of wooden buildings in Japan and have been considered to use chelator-mediated Fenton (CMF) reaction in concert with hydrolytic and redox enzymes for degradation of wood cell wall. CMF can be described as a non-enzymatic degradation system that utilizes hydroxyl radicals pro...
R R Kondo, Y Horikawa, K Ando, B Goodell, M Yoshida


Decay capacity and degradation patterns of Xylaria hypoxylon on different wood species
2022 - IRG/WP 22-10985
A host of physical and environmental factors may influence fungal decay including the wood substrate, temperature, moisture, oxygen, light, pH, and nitrogen. Understanding the effects of these factors on fungal decay is important for the effective utilization of wood decay fungi in biotechnological processes and for understanding the role of these organisms in global carbon cycling. The ascomycete...
E Bari, G Daniel, A Singh, J J Morrell


Changes in topochemistry and mechanical properties of Beech (Fagus orientalis L.) by natural fungus infestation
2022 - IRG/WP 22-10986
Beech is one of the major hardwood species in Europe. It is, however, highly susceptible to fungal attack both in the fresh state and during the storage. Understanding the alteration in chemical and mechanical properties of beech wood during the initial degradation state can lead to improved raw material utilization. Therefore, UV-microspectrophotometer (UMSP) and High-Performance Liquid Chromatog...
H Sivrikaya, M Rehbein, F Divos, S Adamopoulos, R Hosseinpourpia


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to dete...
E E Soytürk, S N Kartal


Steam Pre-conditioning Treatment Prior to Acetylation: Impact on Dimensional Stability, Moisture Response Behaviour, and White-Rot Fungal Resistance of Hevea brasiliensis and Mitragyna ciliata Wood
2022 - IRG/WP 22-40930
The effect of steam pre-conditioning treatment on the dimensional stability, moisture response behaviour, and durability against white-rot fungus P. chrysosporium of acetylated Hevea brasiliensis and Mitragyna ciliata wood species were assessed. Defect-free specimens of both species from the top, middle, and base positions were selected, prepared according to ASTM D143-09 & ASTM D-2017 standards a...
E Uchechukwu Opara, J Mayowa Owoyemi, J Adeola Fuwape


A novel cellulose-binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11019
Wood-rotting basidiomycetes are the major organisms decomposing wood in nature. They are classified into two groups based on their decay modes; white-rot fungi and brown-rot fungi. White-rot fungi secrete various cellulolytic enzymes during the wood degradation process. The enzymes are known to be often appended with a cellulose binding domain (CBD) which assists the activity of catalytic domain. ...
Y Kojima, N Sunagawa, M Aoki, M Wada, K Igarashi, M Yoshida


The cellulose binding mechanism of a novel cellulose binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11021
In nature, wood decay is caused by various wood-rotting basidiomycetes. Wood-rotting basidiomycete are mainly divided into white-rot fungi and brown-rot fungi. Their main carbon source is cellulose of the wood cell wall during wood decay, and they produce a variety of enzymes to decompose cellulose. The cellulolytic enzymes often possess a cellulose binding domain (CBD) as an additional domain con...
M Aoki, Y Kojima, M Wada, M Yoshida


Research on gaseous COS degradation by brown-rot fungus Gloeophyllum trabeum
2024 - IRG/WP 24-11033
Physiological studies of wood rotting fungi have mostly focused on the metabolism of carbon and nitrogen sources, which constitute the dominants components of wood. On the other hand, despite the physiological importance of trace elements such as sulfur, studies on their acquisition sources and metabolic pathways are limited. Until now, wood rotting fungi have been thought to utilize slight amount...
R Iizuka, R Tsukida, Y Katayama, M Yoshida


Characterization of cell wall morphology and water-soluble extractives in Japanese larch decayed by white-pocket-rot fungi
2024 - IRG/WP 24-11034
Porodaedalea chrysoloma (Fr.) Fiasson & Niemelä is a basidiomycete that can cause white-rot decay in Japanese larch (Larix kaempferi) heartwood. P. chrysoloma is a white-rot fungus with a unique pattern that causes white-pocket-rot in the xylem of Japanese larch. However, the mechanism by which P. chrysoloma yields the pattern of white-pocket-rot in Japanese larch heartwood remains unclear. Thus,...
Y Mori, H Arai, T Ito, H Hashitani, K Yamashita, Y Ota, M Kiguchi


A novel cellulose-binding domain from the brown-rot fungus that can be used to evaluate cellulose in wood
2024 - IRG/WP 24-11046
Wood-rotting basidiomycetes are the primary microorganisms that decay wood in nature. They are classified as white-rot fungi and brown-rot fungi by the difference in decaying types. White-rot fungi secrete a variety of cellulolytic enzymes during wood degradation. These enzymes often have an additional cellulose-binding domain (CBD) that adsorbs to the cellulose surface and localizes the catalytic...
Y Kojima, N Sunagawa, S Tagawa, T Hatano, S Nakaba, M Aoki, M Wada, K Igarashi, M Yoshida


The potential of Wacapou (Vouacapoua americana) extracts to develop new biobased protective solutions for white woods
2024 - IRG/WP 24-20724
The valorisation of Amazonian wood residues into active chemical compounds could be an eco-friendly, cost effective and valuable way to develop wood preservatives formulations in order to enhance the decay and termite resistances of low durable wood species, by impregnation processes. Wacapou (Vouacapoua spp., Fabaceae) is a well-known Guianese wood species for its use in local wood construction ...
E Kieny, K Candelier, L Milhe, Y Estevez, C Sophie, R Lehnebach, J Damay, D Florez, E Hoël, M-F Thevenon, J Bossu


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