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Extracellular substance from the white rot basidiomycete Irpex lacteus involved in wood degradation
1992 - IRG/WP 92-1571
Cellulases, phenol oxidase, and a substance that both produced and reduced H2O2 were isolated from cultures containing wood or glucose on which the white-rot fungus Irpex lacteus was growing. The rate of wood degradation by the fungus with different amounts of glucose in the medium was measured. More of the substance that produced and reduced H2O2 was found extracellularly in cultures containing w...
H Tanaka, T Hirano, G Fuse, A Enoki


Premature failure of CCA treated vineyard posts from brown rot
1993 - IRG/WP 93-10016
A survey of CCA (AWPA Type C) treated posts used as grape vine trellis supports, to determine the extent and type of decay was carried out at a terraced vineyard in the Hawkes Bay region of the North Island of New Zealand. Of 357 posts examined out of a total of 970 in the vineyard, 47% had moderate to severe decay or had failed, 20% had established decay, 10% had a trace of decay and 23% had no s...
R N Wakeling, A P Singh


The role of oxidation in wood degradation by brown-rot fung
1992 - IRG/WP 92-1562
Brown-rot fungi are suggested to degrade cellulose by oxidation with hydrogen radicalsformed eg. in the conversion of hydrogen peroxide in the Fenton type reactions. The stuctural changes in the holocellulose in wood induced by Fenton's reaction on wood components are very similar to those caused by brown-rot fungi. In this work the effect of the Fenton reaction on wood components was stu...
A-C Ritschkoff, J Pere, J Buchert, L Viikari


Study of the degradation caused by micro-organisms in Pinus sp. waterlogged wood
1989 - IRG/WP 1411
So far, the different Centers are trying the restoration and the conservation of wood structures, coming from subaquatic archeological deposits, with interest from the historic - artistic point of view. The main objective of this paper has been the determination of the decay level of Pinus sp. wood coming from a roman ship (approximately 2000 years old), where we have analyzed their physical prope...
M T De Troya, M C Escorial, J Garcia, A Cabanas


Effect of some resin treatments on fungal degradation reactions
1999 - IRG/WP 99-10318
The effect of impregnation of wood with vegetable oil, tall oil and two water soluble on fungal degradation reactions was assessed by hydrolysis and enzyme activity measurements. The experiments were carried out by using isolated enzyme preparations and a living brown-rot fungus Poria placenta. The results indicate that the inhibitory effect of treatments was clearly directec towards the enzymatic...
A-C Ritschkoff, M Rättö, A J Nurmi, H Kokko, A O Rapp, H Militz


Resistance of acetylated wood to biological degradation. Evaluation of field test
1997 - IRG/WP 97-30139
Acetylated wood samples were tested in ground contact (stake test) at two test fields, one in Simlångsdalen, Sweden, and one in Viikki, Finland, according to European standard EN 252. The test samples were inspected annually and their condition was compared with that of untreated controls and of samples treated with two reference CCA preservatives. The use of untreated controls and preservative t...
P Larsson Brelid, R Simonson, Ö Bergman


Tunnelling bacteria
1983 - IRG/WP 1186
The micromorphology of decay in wood caused by a certain group of single-cell bacteria, called tunnelling bacteria (TB), is described. TB are characterised by their tunnelling ability within the secondary cell walls of wood fibres. Pure cultures of TB have not yet been obtained although the typical decay patterns can be reproduced using mixed bacterial cultures. Some indications of lignin degradin...
T Nilsson, G F Daniel


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc


Microbial decay in an extremely durable Malaysian hardwood Belian (Eusideroxylon zwageri) - an overview
1997 - IRG/WP 97-10216
The heartwood of Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods, and is therefore a choice timber for soilcontact use in the humid tropics such as untreated telecommunication poles, fence posts and railway sleepers. The high microbial resistance of the wood is evidenced by the mere superficial degradation observed in some pole samples after 20 yea...
A H H Wong, A P Singh


Assessing the importance of degradation mechanisms on the loss of effectiveness of wood preservatives
2000 - IRG/WP 00-20193
Accelerated ageing systems developed for application to samples in the laboratory prior to biological tests, should reflect those natural deterioration processes that are likely to occur in the hazard classes defined in EN 335-1. Losses through evaporation or the effects of leaching have been recognised, however their importance, relative to other mechanisms has not been quantified. Degradation me...
E D Suttie, R J Orsler, T Dearling


Bacterial degradation of Pinus radiata compression wood
1996 - IRG/WP 96-10153
An inspection of twelve year old, CCA-treated Pinus radiata wood from an industrial cooling tower showed extensive surface decay of wood. Electron microscopic examination of decaying compression wood indicated that compression wood cells were attacked exclusively by bacteria, which were of erosion and tunnelling types. As compared to the normal wood, compression wood appeared to be more resistance...
A P Singh, R N Wakeling


Properties of cellulose degraded by the brown rot fungus, Postia placenta
1988 - IRG/WP 1350
To gain further understanding of the nature of the cellulose depolymerizing agent or agents of brown-rot fungi, brown-rotted cellulose was chemically and physically characterized. Various culture conditions, such as low nitrogen and elevated oxygen levels, did not induce degradation of cellulose by Postia placenta in liquid cultures. Therefore, brown-rotted cellulose for analysis was prepared by a...
T L Highley, R E Ibach, T K Kirk


Dimensional stabilization and decay resistance of wood treated with brown-rotted lignin and copper sulfate
1990 - IRG/WP 3608
The objective of this study was to evaluate the potential usefulness of brown-rotted lignin (BRL) as a dimensional stabilization and copper complexing agent for wood treatment. For dimensional stabilization, aqueous solutions of the lignin extract were combined with either copper sulfate, glyoxal or other additives. Anti-shrink efficiency (ASE) values as high as 42% were obtained with wood treated...
L Jin, D D Nicholas, T Schultz


Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner


Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster


Influence of variable lignin content amongst hardwoods on soft-rot susceptibility and performance of CCA preservative
1982 - IRG/WP 1151
This paper presents the outline of an argument, based on both new and established data, which relates varying lignin content to variation of soft-rot susceptibility between hardwood species and of performance of CCA preservatives in controlling soft-rot. Although more data need to be generated to prove this relationship, sufficient evidence exists for the hypothesis to be given close attention, pa...
J A Butcher, T Nilsson


Experiments on the degradation of tributyltin oxide: A progress report
1984 - IRG/WP 3287
A variety of experiments designed to assess the chemical and physical factors affecting the degradation of tributyltin oxide in treated timber are described. Simple procedures in which temperature and oxygen availability were increased in the presence of wood and water suggest that the wood itself was of prime importance. Attempts to decrease degradation with antioxidants were unsuccessful but led...
R J Orsler, G E Holland


Deterioration of wood viewed from iron nail
1991 - IRG/WP 2368
Deterioration of nail was graded by a five - rank numerical - rating. The rating of nail moisture content and decay of wood were inspected in a mortal-wall of 34-year-old house. The wood was mostly in decay, when the rating of the nail was above about 4, and the moisture content was above about 20%. Nailed wood specimens kept in several humidity room of 20°C for 4 years showed that the rating of ...
H Imamura


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


Weathering of copper-amine treated wood
2000 - IRG/WP 00-40155
Copper ethanolamine (Cu-EA) treated southern pine (SP) were artificially weathered with a QUV Weathering Tester. The weathering regime was composed of a continuous UV-light irradiation for 2 hours followed by a water spray for 18 minutes for a total time of 1200 hours. The changes on the surface of the weathered samples were characterized by Fourier transform infrared spectroscopy (FTIR) analysis,...
J Zhang, D P Kamdem


Termiticide degradation in Mississippi field tests
1994 - IRG/WP 94-30052
Degradation rates of some currently marketed United States termiticides are currently being studied. Separate quantities of soil were each treated at lowest label rates with termiticide, and placed in trenches along the inside and outside of concrete walls. Composite soil samples, each consisting of four soil cores, were collected after 1, 30, 60, 120, 180, 240, and 365 days, and again at 2 and 3 ...
B M Kard


Application of non-destructive techniques (durometric and ultrasonic) to evaluate the degradation of woods in service by Gloeophyllum trabeum
2001 - IRG/WP 01-20220
In order to establish the degree of degradation of wood used in construction, it is necessary to employ non-destructive methods (ultrasonic, durometric, densitometric). In this study, ultrasonic and Pilodyn durometric techniques have been applied to try to establish parameters of relationship between the values obtained by both, for their immediate application to wood in service. As a method of re...
M T De Troya, L Palaia, A Navarrete, V Galvañ, R Molina, A Guijarro, J Camacho


Ultrastructure of Prumnopitys ferruginea wood from a buried forest in New Zealand
1991 - IRG/WP 1489
The buried wood of Prumnopitys ferruginea, a New Zealand native tree, was examined by scanning and transmission electron microscopy to investigate the extent to which the wood had deteriorated and the cause of its deterioration. Examinations showed that some deterioration of wood had occurred. Microscopy revealed a decay pattern similar to that produced by cavitation bacteria. Lenticular cavities ...
A P Singh, T Nilsson, G F Daniel


16S rRNA Analysis of the Bacteria Associated with Biocide Degradation
2004 - IRG/WP 04-10543
The bacterially mediated degradation of the new anti-fungal biocide, bethoxazin, was studied in vitro, by means of 16S rRNA PCR-amplification and cloning techniques. Woodblocks impregnated with a subtoxic concentration of bethoxazin were incubated in compost, and the micro-organisms associated with the wood after 6 and 12 weeks was studied. It was found that after 6 weeks the wood contained a la...
D F Wallace, D J Dickinson


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