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Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp


Minimum inhibitory concentration of methylene bisthiocyanate towards selected wood inhabiting fungi in liquid culture and on radiata pine
2001 - IRG/WP 01-30261
The objective of the current study was to determine the minimum inhibitory concentration (MIC) of methylene bisthiocyanate (MBT) towards wood inhabiting fungi common on unseasoned radiata pine (Pinus radiata D. Don). The fungi used were Ophiostoma floccosum, Leptographium procerum, Sphaeropsis sapinea and Trichoderma viride. Fungi were individually inoculated into nutrient media spiked with differ...
T Singh, B Kreber, C Chittenden, R N Wakeling, A Stewart


The durability of wood polymer composites against fungi and insects
2000 - IRG/WP 00-40161
New materials such as wood polymer composites are used more in France. These materials are obtained by mixing wood and thermoplastics in different ratios. These kind of material must be considered as new materials and some characteristics such as durability must be evaluated for outdoor applications. On the other hand, some recycled fibers from wood waste could be used as raw materials. Some woodp...
G Labat, I Le Bayon, J Gerard, F Amin


Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt


The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Effects of the specimen position on fungal colonisation and wood decay by en 113 test fungi
1998 - IRG/WP 98-20136
For testing wood preservatives according to EN 113 it is common practice to plant the test blocks on neutral supports in order to prevent (1) a diffusion of chemicals into the agar medium and (2) an excessive moistening of the specimens. The procedure was employed in EN 350-1 for testing the natural durability of solid wood. It turned out to be of problematic nature because of the individual requ...
G Kleist, M-T Lenz, R-D Peek


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce


Chemostimulatory and chemotropic responses by fungi to preserved and unpreserved wood
1981 - IRG/WP 1134
During experiments to determine the presence of biotic connections between soil and wood at this laboratory, marked chemotropic responses to wood were demonstrated by some wood destroying micro-organism in agar culture. These were shown at some distance from bait, up to 35 mm away, and on some occasions were unaffected by the presence of toxic materials. This paper provides preliminary data on the...
G Mowe, B King


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


Northern oak wood and its damages in Iran
1988 - IRG/WP 1377
The Northern oak species of Iran designated Quercus castaneaefolia C.A.M. is distributed widely in caspian littoral. In natural conditions (healthy) has favourable quality and it is used mostly in rural construtions, sleepers, parquets, cross arms for electric and communication poles, it is used also indoor and window construction, benches and finally veneer. This wood in the case of sleepers and ...
D Parsapajouh, P Niloufari


Isolation and identification of the fungal flora in treated wood. Revised technique
1977 - IRG/WP 159
At the 8th Annual Meeting in Wildhaus a paper was presented for discussion on the isolation of fungi from treated wood.·Since then work of this nature has been undertaken at Imperial College and as a result a revision of that document has been made and is presented here. The main alterations are: 1) To streamline the isolation procedure 2) Modification of the benomyl agar 3) The inclusion of a st...
C P Clubbe, J F Levy


Influence of the decay of spruce chips by the selected fungi on their chemical structure and the pulp properties
2004 - IRG/WP 04-10510
The paper focuses on changes in the weight and in the molecular structure of spruce chips submitted to long-term storing as well as to medium- and long-term model rotting degradation caused by some chosen fungi identified at storing processes on the pile. For the model decay of chips under laboratory conditions during 3 or 6 weeks the white-rot fungi: Phanerochaete chrysosporium, Heterobasidion an...
R Solár, L Reinprecht, A Geffert, F Kacík


Evaluation of tropolone as a wood preservative : activity and mode of action
2002 - IRG/WP 02-30282
The fungicidal activity of 2-hydroxy cyclohepta-2,4,6-trienone (tropolone) analogue of b-thujaplicin a natural product responsible for the durability of heart wood of several cupressaceous trees was investigated in vitro on growth of white and brown rot fungi. Results obtained show that tropolone, easily prepared from commercially available products, possesses strong fungicidal activity similar ...
P Gérardin, M Baya, N Delbarre, P N Diouf, D Perrin, P Soulounganga, E Gelhaye, J P Jacquot, C Rapin


The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell


A field test on susceptibility of wood-based board materials to moulds
1989 - IRG/WP 3545
A field test on susceptibility of wood-based board materials to mould growth was carried out under practical, but severe, service-conditions. A total of 19 commercially available composite boards were investigated. It was found that all board materials tested were not completely immune to mould attack. Test results revealed that among the three main categories of wood-based boards involved, partic...
Qiao Wang, B Henningsson


Biological resistance of wood-based composites under protected, aboveground conditions
2005 - IRG/WP 05-20312
Five kinds of wood-based composites (density fiberboard=MDF, hardwood plywood=HP, softwood plywood =SP, particleboard=PB and aspen oriented strand board=OSB) were tested for their resistance against subterranean termites and decay under protected aboveground conditions. The exposure method was previously developed for evaluating performance of sill plates (dodai) in Japanese homes. Ten replicates ...
K Tsunoda


Uptake of copper by mycelium of wood decay fungi growing on copper S-substituted thioglycolate containing nutrient media
1998 - IRG/WP 98-10291
Mycelia of Trametes versicolor, Coniophora puteana and Poria monticola were grown on potato dextrose agar (PDA) media, containing various concentrations of copper N,N-dimethyldithiocarbamoylacetate (which may be regarded as a copper S-substituted thioglycolate). The tested copper compound revealed relatively low fungicidal activity. After 13-18 day growing period, we determined concentrations of a...
M Humar, M Petric, F Pohleven, P Kalan


A comparison of fungal strains used in the bioassay of wood preservatives
1984 - IRG/WP 2220
Previously published data are presented relating to a number of strains of wood-destroying basidiomycetes (Coniophora puteana, Coriolus versicolor, Gloeophyllum abietinum, Gloeophyllum sepiarium, Gloeophyllum trabeum, Lentinus lepideus, Poria placenta, Fibroporia vaillantii and Serpula lacrymans) commonly used as test fungi in the bioassay of wood preservatives. The data, which has not been statis...
J Wazny, H Greaves


Exposure of preservative treated wood to terrestrial microcosms, pure cultures of fungi and in the field
1997 - IRG/WP 97-20114
Small stakes (5x10x100 mm3) of treated and untreated Pinus sylvestris sapwood were exposed in terrestrial microcosms with different soils, two taken from test fields in Sweden, one soil from a conifer forest and one garden compost soil. Stakes of the same size were also exposed to pure cultures of the brown and white rot fungi, Postia placenta and Phanerochaete chrysosporium. After exposure, weigh...
M-L Edlund


Field test of wood preservatives with Nasutitermes rippertii RAMBUR in Cuba
1996 - IRG/WP 96-10181
A field test of wood preservatives was carried out with Nasutitermes rippertii RAMBUR in the vicinity of the Material Testing Centre „Alexander von Humboldt" in Santiago de las Vegas/ Havana (Cuba) for 12 and 24 months. Test specimens of Pinus sylvestris L., Picea abies (L.) Karst. and Fagus sylvatica L. with dimensions of 25 x 25 x 490 mm³ were arranged vertically or horizontally in a circle o...
W Unger


Wood preservation research in Tanzania: Priorities and challenges
1988 - IRG/WP 3462
Tanzania provides some of the highest rates of wood deterioration in service leading to alarming economic losses and yet no serious concern is shown in search for solutions to the problem. The paper attempts to point out some of the priority areas in the field of wood preservation research that demand urgent attention if timber is to be used judiciously in this country. An account is given of the ...
K K Murira, R Cockcroft


Screening of fungal strains for wood extractive degradation
1998 - IRG/WP 98-10254
Fungal strains were screened for their ability to degrade apolar extractives in wood from scots pine. The degradation of total wood extractives by 91 different strains was monitored in stationary batch assays incubated for 6 weeks. The results obtained show that the ability of wood-inhabiting fungi to utilize wood extractives varied greatly, even for different isolates of the same species. Fungal ...
J Dorado, M J Martinez-Inigo, T A van Beek, F W Claassen, J B P A Wijnberg, R Sierra-Alvarez


Wood injurers found at wood processing plants in SR Sloveni
1981 - IRG/WP 1140
This report describes the stating of damages on wood and taking stock of wood injurers found in the warehouses of wood processing plants in SR Slovenia. The results of this research show that in the process of manufacture vast quantities of spruce-wood, pine-wood, beech wood, oak-wood, and poplar-wood are exposed to numerous injurers, both fungi and insects. The reasons for such a state lie in con...
R Benko


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