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Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson


PXTS; A Metal Free Oligomer Wood Preserving System - A Summary of Data To Date
2004 - IRG/WP 04-30350
The world is continually looking for new wood preservative technology, especially to address environmental concerns. Recent changes in the USA have limited the use of arsenical containing formulations to industrial use through a negotiated voluntary cancellation of most residential label uses. PXTS (Polymeric Alkylphenol Polysulfide) offers a brand new technology that is an all organic system, lik...
M H Freeman, D D Nicholas, D Renz, R Buff


Evaluation of decay fungi in Norwegian buildings
2005 - IRG/WP 05-10562
During a period of 2 years and 3 months (1 January 2001 - 20 March 2003) Mycoteam had 3161 consultations in buildings in southern Norway, 1428 revealing damage from decay fungi. One consultation often revealed several occurrences of fungi, and the total number of occurrences of decay fungi was 3434. Thirty-five different species/genera/groups of decay fungi were recorded. During this period brown ...
G Alfredsen, H Solheim, K Mohn Jenssen


Effects of heart wood extractives in Dahoma (Piptadeniastrum africanum) on decay resistance to white- and brown-rot fungi
2004 - IRG/WP 04-10536
Dahoma (Piptadeniastrum africanum) is a durable but a lesser utilised tropical hardwood species from Ghana that has the potential of being used as an alternative to Iroko (Milicia excelsa) for many end uses. However, the timber gives off a strong odour especially when wet and during processing. The high durability and the strong odour of the timber could be due to the presence of extractives in th...
Zeen Huang, K Maher, S A Amartey


The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson


Some Experiences with Stake Tests at BAM Test Fields and in the BAM Fungus Cellar Part 1: Comparison of Results of Visual Assessments and Determinations of Static Moduli of Elasticity (MOE)
2005 - IRG/WP 05-20319
With examples of routine in-ground stake tests differences are shown in the performance of wood preservatives at the BAM test fields Lehre and Horstwalde and in the BAM fungus cellar. Signs of attack of micro-organisms were assessed visually according to EN 252. Periodical determinations of static moduli of elasticity (MOE) revealed the influence of the attack on the elastic properties of the wood...
M Grinda, S Göller


A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


The role of oxalic acid in short fiber formation by the brown-rot fungus Postia placenta
1993 - IRG/WP 93-10028
The mechanism by which brown-rot fungi depolymerize wood and cellulose remains a perplexing problem. Current evidence favors oxidation by low-molecular weight, non-enzymatic fungal metabolites. During attack of cotton cellulose by Postia placenta MAD-698, the degree of polymerization (DP) decreases to limit of degree of polymerization (LODP) over a period of 5-6 weeks with relatively low weight lo...
F Green III, J M Hackney, C A Clausen, M J Larsen, T L Highley


Biological variability in the oxalate/oxalate decarboxylase system among five isolates of the wood-degrading fungus Meruliporia incrassate
2006 - IRG/WP 06-10573
The “dry-rot” wood decay fungus Meruliporia incrassata has recently attracted attention, primarily in the western coastal United States, as a particularly destructive pest of building material. Recently, the US Environmental Protection Agency (EPA) has accepted a voluntary withdrawal of the historically effective chromated-copper arsenate (CCA) as a preservative for wood used in residential se...
C Howell, J Jellison


Micromorphological Characteristics of Degradation in Bamboo Attacked by White Rot Fungus Lentinus edodes
2006 - IRG/WP 06-10575
In comparison with micromorphological works for wood decay, little known is about the decay pattern of bamboos by wood decay fungi. The present work was undertaken to understand the general degradation pattern of bamboo and the influence of polylaminate layers in bamboo fiber walls on the restriction of fungal decay. The weight loss of bamboo species Phyllostachys puberscens against the white rot ...
Chang Hyun Cho, Kwang Ho Lee, Yoon Soo Kim


Micromorphology of Bamboo Fibers Degraded by Brown-Rot Fungus Gloeophyllum trabeum
2006 - IRG/WP 06-10576
The decay pattern of bamboo by brown-rot fungus was examined. In addition, the influence of polylaminate structures in bamboo fibers on the restriction of fungal decay was also investigated. The weight loss of bamboo Phyllostachys puberscens by the brown-rot fungus Gloeophyllum trabeum after 16 week incubation was about 25%. Parenchyma cells were severely degraded. Microscopical studies showed tha...
Kwang Ho Lee, Chang Hyun Cho, Yoon Soo Kim


Laboratory Evaluation of Soft Rot Resistance of Non-Durable Lesser-Known Malaysian Hardwoods
2006 - IRG/WP 06-10582
Resistance of six non-durable lesser-known Malaysian hardwoods, Pulai (Alstonia spp.), Rubberwood (Hevea brasiliensis), Ludai (Sapium spp.), Jelutong (Dyera costulata), Gaham Badak (Blumeodendron tokbrai) and Kayu arang (Diospyros spp.) compared with temperate Scots pine sapwood (Pinus sylvestris), were evaluated using the unsterile soil burial (mixed with Chaetomium globosum) versus vermiculite-b...
A H H Wong


Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Resistance of pine and spruce heartwood against decay - The effect of wood chemical composition and coating with water-borne wood oil product
2006 - IRG/WP 06-10597
Natural durability of wood has been widely studied, but the combination of the natural durability and different treatments has not been the focus of many studies. The durability of wooden products is mainly based on the water permeability and the resistance against organisms. In this study, the water absorption and decay resistance of sapwood and heartwood of Scots pine and Norway spruce were exam...
H Viitanen, S Metsä-Kortelainen, T Laakso


Biological resistance of didecyl dimethyl ammonium tetrafluoroborate (DBF)-treated wood in soil-bed and Basidiomycetes tests
2006 - IRG/WP 06-30393
This study evaluated the decay resistance of treated wood with a new quaternary ammonia compound, didecyl dimethyl ammonium tetrafluoroborate (DBF) in laboratory soil bed and Basidiomycetes tests. Treated sugi sapwood specimens with DBF at various concentration levels were first subjected to soil bed tests using two types of soil. The specimens subjected to soil bed tests were then used for Basidi...
S N Kartal, C Brischke, A O Rapp, Y Imamura


Protection of hard and softwood through Neem leaves extracts and oil - A direction towards development of eco-friendly wood preservatives
2006 - IRG/WP 06-30394
Conventional wood preservatives like CCA and CCB are facing lot of criticism all over the world. It is essential to address the problem in view of environment protection. Eco-friendly wood preservatives may be considered as one option. The present study is an expolartion of neem leaves and seed oil against wood decaying fungi and termites. Azadirachta indica A. Juss, commonly known as neem is one ...
S Dhyani, S Tripathi


Beetle-Fungus Associations in Woody Substrates in the Context of International Trade
2007 - IRG/WP 07-10610
Interest in the role of insects in transmitting various fungi and in the potential for outbreaks of insects vectoring plant disease organisms has resulted in a large volume of literature on insect-fungi relationships. Mites are mentioned as an important third partner in these associations. This paper summarizes information and provides an update on major aspects of these intricate associations in ...
A Uzunovic


Phenol oxidase activity and one-electron oxidation activity in wood degradation by soft-rot deuteromycetes
2007 - IRG/WP 07-10615
Wood degradation, one-electron oxidation activity as assayed by ethylene generation from 2-keto-4-thiomethylbutyric acid (KTBA), and phenol oxidase activity were measured in cultures of six deuteromyce fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing...
H Tanaka, M Yamakawa, S Itakura, A Enoki


Gypsum effects on ‘dry rot’ wood degradation as a function of environment
2007 - IRG/WP 07-10624
‘Dry rot’ fungi are a unique group of brown rot fungi that can degrade wood away from ground contact where other fungi fail to colonize. Successfully occupying this niche is partially due to efficient water and nutrient transport, but mobilizing elements, notably calcium (Ca) and iron (Fe), from adjacent building materials has also been implicated in their success. Here we report a series of t...
J Schilling, J Jellison


Chelator-Mediated Fenton Chemistry in Wood Degraded by Fungi
2007 - IRG/WP 07-10618
Wood specimens were colonized by individual isolates of brown rot, white rot, soft rot and blue (sap) stain fungi. Ethyl acetate extracts of the ground wood were analyzed for their iron-reducing capabilities using a ferrozine-based assay. Extracts from wood colonized by brown rot fungi showed a significantly greater iron-reducing capability than extracts from wood colonized by white rot fungi or n...
B Goodell, G Daniel, J Jellison, Yuhui Qian


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


Genome-wide survey of cellulase related genes of white rot fungus,Pleurotus ostreatus
2007 - IRG/WP 07-10627
A white rot fungus, Pleurotus ostreatus, which is a important edible mushroom, has received much attention to apply to bioremediation and bioconversion because it has both cellulase and ligninase. To confirm copy number of cellulase-related genes encoded in P. ostreatus genome, we attempted to genomic Southern hybridization of P. ostreatus. The draft genome sequence and a large quantity of EST and...
T Tamenori, S Horisawa


White Rot in Eucalyptus Wood: Anatomical Changes and Density Variation by X-Ray Densitometry
2007 - IRG/WP 07-10631
Wood samples of the hybrid Eucalyptus grandis x urophylla attacked by white rot fungi were characterized. Microscopically, the colonization of the fungi was observed in the vessels and radial parenchyma, however the cell walls of the some fibers were attacked, indicating susceptibility to this fungi. Zone lines were observed as a result of interaction and compartmentalization of the fungi. Microsc...
M Tomazello Fo, S Brazolin, M P Chagas


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