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Lethal temperature for some wood-destroying fungi with respect to eradication by heat treatment
1984 - IRG/WP 1229
The lethal heat dosis as a function of temperature and time of exposure has been tested for mycelium and for wood infested by three strains of Serpula lacrymans and by Coniophora puteana, Poria monticola, Paxillus panuoides, Gloeophyllum trabeum and Gloeophyllum sepiarium. Even for Serpula lacrymans, which is well known for its sensivity to heat treatment, 30 min at 55°C were necessary to kill th...
M Miric, H Willeitner


Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates
2003 - IRG/WP 03-10475
D-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase (glucose 2-oxidase) type wi...
T H de Koker, M D Mozuch, P J Kersten


A technique for assessing the preventive efficacy against decay fungi of preservative treatments applied to wood
1988 - IRG/WP 2309
A method is described in which test blocks with envelope preservative treatments can be challenged by selected test fungi previously established on an untreated feeder block. The progress of the test fungus through the treated zone is monitored using novel baits or sensors inserted in holes drilled into blocks to within predetermined distances of the face being challenged. Preliminary results show...
J K Carey, A F Bravery


The antifungal efficacy of Guayule resin
1987 - IRG/WP 3429
The Naval Research Laboratory is evaluating the non-rubber-producing portion of guayule (Parthenium argentatum) resin as a protectant for wood in terrestrial and marine service. This study phase, in collaboration with the universities of Arizona and Mississippi State, evaluates the resin's fungicidal worth. Resin-impregnated pine sapwood was exposed to brown rot fungi (Gloeophyllum trabeu...
J D Bultman, R L Gilbertson, T L Amburgey, J E Adaskaveg, S V Parikh, C A Bailey


SEM investigation of the production of extracellular mucilaginous material (ECM) by some wood inhabiting and wood decay fungi when grown on inert surfaces
1997 - IRG/WP 97-10194
A range of wood inhabiting and wood decay fungi have been studied for their production of extracellular mucilage when grown on inert surfaces, for comparison with their growth on wood. The species tested were: Coniophora puteana, Coriolus versicolor, Chaetomium globosum, Trichoderma harzianum, Ceratocystis coerulescens, Ophiostoma piceae, Mucor sp. and Penicillium sp. They were grown on plastic me...
A R Abu, R J Murphy, D J Dickinson


Wood colonizing fungi as a human pathogen
1992 - IRG/WP 92-1523
Today we recognize some 174 "pathogens" among the approximately 100000 species of fungi, which cause different diseases on man. 24 fungi which are well known as wood colonizing fungi have been identified as opportunistic human pathogen. Some of them have been found on the surface of the human body, causing superficial mycoses, or cutaneous infection, like dermatophytosis or dermatomycosis. Other c...
R Benko


Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10210
In this work, the in vitro species of Pleurotus (P.eringii, P. cornucopiae and P. ostreatus), from structures of degraded wood have been studied. This degradative activity was compared to those presented by Trametes versicolor (fungus characteristic of white rot) and Gloeophyllum trabeum (fungus representative of brown rot). The activities studied were the following: cellulase, xylanase, pectinase...
D Muñoz-Mingarro, F Llinares, M T De Troya, F Rubio, M Yuste, C Rodríguez-Borrajo, J E Garcia de los Rios, F Alvarez, P Jiménez, A Rojas, A Navarrete, P Reche


Isolation of soil borne bacteria and fungi from treated timber
2001 - IRG/WP 01-50174
Most research in the last few decades has focused on the development of new strategies to control biological attack and the means to quantify this. Comparatively little work has been done to examine the effect that treated timber might have on its surrounding environment. This presentation will describe a methodology that attempts to detect any changes which might occur in the soil microflora foll...
I Stephan, A Stegemann, G Heidrich


Chlorothalonil: A new ground contact wood preservative
1992 - IRG/WP 92-3712
Extensive investigations have been conducted in recent years on the performance of chlorothalonil (tetrachloroisophthalonitrile) as a ground contact wood preservative. Efficacy information is presented on a soil block test with 11 Basidiomycetes, a fungus cellar evaluation, above-ground field testing against termites in Australia and Hawaii, and ground-contact field stakes installed in Florida (9 ...
P E Laks, T L Woods


Evidence for the participation of the osmiophilic particles in the enzymatic demethylation of lignin
1984 - IRG/WP 1239
Transmission electron microscopic observations of the white-rot fungi Trametes hirsuta and Phanerochaete chrysosporium grown on a series of wood pulp with a graded lignin content and mycelium from the clarified centre of an agar-medium containing lignin (E1-effluent from the bleaching process) showed that osmiophilic particles are only induced by native lignin. Taking into account that the osmioph...
K Messner, H Stachelberger


Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley


Degradation of lignin model compounds with coordinated copper in the presence of peroxide
1998 - IRG/WP 98-10282
A novel diffusible system capable of decomposing lignin model compounds with copper, coordinators and peroxides has been proposed. When pyridine was used as a copper coordinator, two synthetic dyes, Poly-R and RBBR were intensively decolorized with Cu(II) and H2O2 in aqueous solutions at room temperature. Although reactions with Cu(II) and H2O2 produce a strong oxidant, hydroxyl radical, via a Fen...
K Koller, K Messner, T Watanabe


Soft-rot control in hardwoods treated with chromated copper arsenate preservatives. Part 3: Influence of wood substrate and copper loadings
1977 - IRG/WP 2100
The hypothesis is proposed that hardwoods need more chromated copper arsenate (CCA) than softwoods to protect them from soft-rot attack mainly because hardwoods are more readily consumed by soft-rot fungi. Simple model systems, using copper-supplemented agar or groundwood pulp treated with CCA showed that fungi tolerated more toxicant (copper) as more available substrate (malt) was provided. Soft-...
M A Hulme, J A Butcher


Contribution to the knowledge of wood-rotting fungi in Brazil. Part 1: Occurrence and distribution of fungi on different substrates from the Manaus region, Amazonas State
1995 - IRG/WP 95-10096
A total of 945 spezimens of fungi were studied with regard to their distribution on ditterent substrates, abundances and economic importance. The samples came from the collection ot the Wood Pathology Laboratory of CPPF/INPA in Manaus, Brazil. Altogether, 163 spezies and 87 genera were identified. The following genera Trametes, Auricularia, Panus, Rigidoporus and Hymenochaete were represently by m...
M Aparecida de Jesus


Preservative-efficacy of boric acid-triethanol amine solution against wood-decay fungi
1994 - IRG/WP 94-30050
Laboratory preservative-efficacy tests were conducted using boric acid-triethanol amine (BTEA) solution in accordance with the JIS A 9201 (1991) test method excluding the standard weathering process. Sapwood specimens of Picea jezoensis or Fagus crenata to achieve nominal retentions of 0.40-41.2 kg/m³ of boric acid were exposed to Tyromyces palustris, Coriolus versicolor, Serpula lacrymans or Cha...
S Doi, M Mori, Y Mineki


Antifungal properties of metabolites produced by Trichoderma isolates from sawdust media of edible fungi against wood decay fungi
1994 - IRG/WP 94-10051
Trichoderma isolated from a sawdust medium of Pholiota nameko produced filtrates which had antifungal activities to four wood decay fungi tested on the agar plates. Filtrates produced from another isolate, from a sawdust medium of Lentinus edodes, had antifungal activities only to the white rot fungi, Coriolus versicolor and Pycnoporus coccineus. These results did not agree with those from earlier...
S Doi, M Mori


Preservative ability of wood to be fixed hydroxyl apatite substituted for antimicrobial metals
2001 - IRG/WP 01-30272
We succeeded in forming hydroxy apatite (HAp) in wood. HAp is non-toxicity and safe. Preservative ability of the wood, which substituted one part of Ca of constituent element of this HA p for antibacterial metals was measured. When Ca was substituted for Ag or Zn, mass loss in decay by brown-rot fungus F. palustris was restrained in about 50% (Ag) ~30% (Zn) of value of control specimen. However, w...
Y Haruhiko, I Sumaru


Destaining wood sapstains caused by Ceratocystis coerulescens
1996 - IRG/WP 96-10159
Fungal sapstain does not reduce the strength of wood, but it does discolor the wood, detracting from its appearance and decreasing the value of wood and wood products. The purpose of this investigation was to assess whether wood sapstain caused by Ceratocystis coerulescens could be destained and existing growth eradicated. The hydroxyl radicals generation under optimized conditions destained the s...
S C Croan


Application of a novel strength evaluation technique during screening of wood preservatives
1986 - IRG/WP 2262
The effectiveness of CCA and ACA in treated aspen mini stakes tested using a novel bag procedure, with unsterile soil fortified with Chaetomium globosum and Ceratocystis albida, is reported. Good agreement between toxic limits determined using the standard weight loss procedure, and those determined by the strength technique were found, with some indication that the strength loss method is more se...
J N R Ruddick


Enhanced biodegradation of cocopeat by soft rot fungi
1998 - IRG/WP 98-10276
Biodegradation of cocopeat (coir dust) was enhanced by the addition of nitrogen (N) fertilizer and inoculation with the soft rot fungus Chaetomium globosum. The N and fungally treated cocopeats had a greater percentage weight loss (27%) after 3 months compared with 7% weight loss without the added fungi and N fertilizer. In addition, their hemicellulose, cellulose and lignin contents were greatly ...
P Y Yau, R J Murphy


Evaluation of chlorpyrifos and fungicides alone and in combination for control of insects and fungi in wood and wood composites
1998 - IRG/WP 98-30187
Wood composites are rapidly being adapted for use in exterior applications. The incorporation of a preservative system to prevent fungus and insect attack is necessary with most exterior composites. Research studies were reviewed pertaining to organic preservative systems based on Lentrek* insecticide wood treatment which contains the active ingredient chlorpyrifos alone and in combination with te...
M P Tolley, P E Laks, R Fears


Soft rot
1978 - IRG/WP 179
Soft rot decay of treated wood is examined with special reference to hardwoods treated with CCA. Factors which adversely affect the chances of protection of hardwoods against soft rot are discussed. The ratio of the volume of the fibre cell wall to the volume of the fibre lumen is presented as a major factor influencing final preservative concentration in the fibre cell wall, the major strength co...
C R Levy


Fungi causing sap stain in wood
1980 - IRG/WP 199
The present paper is a revised edition of former Document No: IRG/WP/125. It contains some additions and an explanation of terminology, as was suggested at a previous meeting of the Group. The paper is a compilation based mainly on available literature. Some of my own unpublished results have been added together with other unpublished data which have been received from the Bundesanstalt für Mater...
A Käärik


Influence of CCA and TnBTO on wood decay, under different oxygen levels and various initial moisture content
2001 - IRG/WP 01-10415
Fagus sylvatica (beech) and Pinus sylvestris (Scots pine) were impregnated by TnBTO (tributyl-tin oxide) and CCA (chromated copper arsenate). In wood blocks two levels of moisture content (50% and 100% of saturation point) were used. Three levels of oxygen concentrations (10, 21, and 50%) were chosen. Treated and untreated wood were exposed to three fungi (C. versicolor a white rot, C. puteana, a ...
S M Kazemi, R J Murphy, D J Dickinson


Molecular studies on house rot fungi by RAPD-PCR
1997 - IRG/WP 97-10195
For genetic information and in view as a possible diagnosis method various isolates of the dry rot fungus, Serpula lacrymans, the wild merulius, S. himantioides, and the cellar fungus, Coniophora puteana, from Asia, Europe and USA were investigated by the polymerase chain reaction (PCR) based technique of randomly amplified polymorphic DNA (RAPD). The banding patterns obtained revealed for S. lacr...
O Schmidt, U Moreth


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