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Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki


Blue Staining of Coated Modified and Unmodified Wood
2006 - IRG/WP 06-10589
We investigated resistance of unmodified, DMDHEU modified and oil heat treated uncoated and coated Scots pine wood against blue stain fungi. Both EN 152-1 and the reverse exposure tests showed excellent resistance of DMDHEU and especially of oil heat treated wood against blue staining. It is not needed to add any additional protective coating layers to prevent modified wood from staining. However,...
M Petric, M Pavlic, M Humar, M Tomažic


The effect of chitosan on the growth and physiology of two wood inhabiting fungi
2006 - IRG/WP 06-10590
The effect of chitosan on the wood inhabiting fungi Sphaeropsis sapinea and Trichoderma harzianum was evaluated at cellular level. Increasing concentrations of chitosan caused an increase in the amount of peroxide in cultures of S. sapinea, which was accompanied by a simultaneous decrease in superoxide. The same effect was not observed in T. harzianum. The growth of both fungi was inhibited when h...
D Vesentini, T Singh


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Direct analysis from wood of the blue stain fungi Aureobasidium pullulans and Hormonema dematioides by denaturing gradient gel electrophoresis
2006 - IRG/WP 06-10595
Aureobasidium pullulans and Hormonema dematioides are the two organisms used in the EN 152 laboratory method for determining the effectiveness of preservatives against blue stain in service. The literature concerning the disfigurement of surface coatings and exposed timber in-service states that A. pullulans is the dominant blue stain fungus, due to its frequent isolation from stained material. In...
M J Ray, D J Dickinson


Decay resistance of coconut and rubber woods. Alternative wood species from Ghana
2006 - IRG/WP 06-10596
With the expanding local and world wide demand for tropical timbers, there is increased interest in the suitability of lesser–used timber species as alternatives for the forest based industries in order to ensure sustainable forest management. In the forests of Ghana, there are quite a number of timber species that are lesser known and have not been adequately used due to the absence of informat...
S Amartey, M Humar, B Donkor, F Pohleven


Resistance of pine and spruce heartwood against decay - The effect of wood chemical composition and coating with water-borne wood oil product
2006 - IRG/WP 06-10597
Natural durability of wood has been widely studied, but the combination of the natural durability and different treatments has not been the focus of many studies. The durability of wooden products is mainly based on the water permeability and the resistance against organisms. In this study, the water absorption and decay resistance of sapwood and heartwood of Scots pine and Norway spruce were exam...
H Viitanen, S Metsä-Kortelainen, T Laakso


The natural durability of wood in different use classes - PART II
2006 - IRG/WP 06-10598
The natural durability of important European wood species has been tested on 3970 speci¬mens in field trials. The wood was exposed at five test sites in Germany with different climates, at each site in European use class 4 and 3 (with and without soil contact). Within European use class 3 three different expositions were tested: vertical with sheltered end grain, vertical unsheltered and horizont...
A O Rapp, U Augusta, K Brandt


Comparing microbial colonisation and Decay Rates of Wood from Sound and Aphid-Killed Kenyan-Grown Mexican Cypress (Cupressus lusitanica)
2006 - IRG/WP 06-10599
Samples of wood from 10, 15, and 30-year old trees attacked and killed by the cypress aphid (Cinara cupressi) and sound Kenyan-grown Cupressus lusitanica trees were investigated for variations in moisture content, density and susceptibility to microbial decay. MC varied with tree age, a normal trend, and between samples from aphid-killed and sound trees. In samples from 10, 15 and 20-year old so...
R Venkatasamy


Study of new wood preservatives from biological origin and low toxicity against wood decay organisms (fungi and termites)
2006 - IRG/WP 06-10600
Wood as an organic material is likely to be attacked by living organisms if no protecting treatment is applied to improve its durability. The biological agents, which in Spain, due to its specific climatologic, cause major damage to wood, are the stain fungi, the rot fungi and the subterranean termites. The commercialisation of the products actually used to treat wood against fungi and to treat t...
M T Troya, M J Prieto, F Rubio, D Lorenzo, J J Teixidó


Termite Response to Oil-Heat-Treated Norway spruce, Scots Pine and Eucalyptus Wood
2006 - IRG/WP 06-20325
The work here presented focused on the behaviour of subterranean termites (Reticulitermes grassei Clément) towards Oil-Heat-Treated (OHT) Norway spruce (Picea abies L.), Scots pine (Pinus sylvestris L.) and eucalyptus (Eucalyptus globulus Labill) wood, with a particular interest in the development of simple methods adequate to study the resistence of these materials to termite attack. Small test ...
L Nunes, T Nobre, C Welzbacher, A O Rapp


The role of chromium in wood preservatives - The situation and new results concerning biological efficacy
2006 - IRG/WP 06-30397
A heavy discussion about the status of chromium-containing compounds in wood preservatives has ensued in the European Union since the Biocidal Product Directive 98/8/EG has come into force.The origin of this discussion is the fact that according to the Biocidal Product Directive chromium trioxide and sodium dichromate have been identified as active ingredients and therefore the placement of wood p...
P Jüngel, H Härtner, E Melcher


Efficacy of some fungicides against mold on freshly cut beech sawn wood
2006 - IRG/WP 06-30403
The quality class of molded wood is lowered and this can result in financial losses due a decrease in its trade value. Currently beech wood is often used without steaming it first and it moulds in the course of drying. The aim of this study was to identify kinetics of beech wood molding and effectiveness of action of chosen modern pro-ecological wood preservatives applied in the protection of fres...
A Fojutowski, A Kropacz


Identification of fungi colonising coated and modified wood exposed outdoors using sequencing and T-RFLP profiling
2006 - IRG/WP 06-20326
Wood decay and staining fungi are known to colonize coated and modified wood. Rapid and reliable ways of identifying fungi on coated and modified wood may improve diagnostics and product development. In this work nine fungi growing in painted wood panels exposed outdoors at a test field in Uppsala, Sweden, were identified using sequencing and T-RFLP (Terminal Restriction Fragment Length Polymorphi...
U Råberg, J Bijelovic, C J Land, S Bardage, N Terziev


Time-of-Flight Secondary Ion Mass Spectrometry Analysis of Polyethylene Glycol Penetration into Wood Cell Walls
2006 - IRG/WP 06-20328
Penetration of high molecular weight polyethylene glycol (PEG) into wood cell walls was investigated in red pine. PEG of different molecular weights (1000, 8000 and 20000) was labelled by bromination and treated samples were analyzed on Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) in dynamic mode. Both negative and positive spectra of the samples were collected in high spatial (bunche...
D Jeremic, P Cooper, P Brodersen


EN 152: Is this Standard Relevant for today’s Wood Preservatives? A Critical Review.
2006 - IRG/WP 06-20331
The paper critically reviews the Blue stain test method described in EN 152 including possible test variables. Whereas EN 152 has been successfully used for solvent borne wood preservatives, it appears that the methodology is not suited for modern water borne products, where the majority fail. The paper will show that there exist different quality claims not concordant with EN 599-1 within diff...
B Jensen, F Imsgard, J Larsen


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl


Moisture correction for ultrasonic MOE measurements above fibre saturation point in Scots pine sapwood
2006 - IRG/WP 06-20333
There is a high correlation between methods for dynamic modulus of elasticity (MOEdyn) and static modulus of elasticity (MOEstat). MOEdyn methods have been found sensitive to detect early stages of decay and may be seen as an option for non-destructive wood durability testing. As the MOEstat measurements do not change after reaching the fibre saturation point, the uncorrected MOEdyn data from ultr...
E Larnøy, G Alfredsen, H Militz


Effect of wood decay on the proportional limit of thin wood samples stressed in the bending mode
2006 - IRG/WP 06-20334
When using bending stiffness as a measure of wood decay it is advantageous to repeatedly test individual samples after they are exposed to decay fungi for varying periods of time. In order to obtain reliable results, variation in modulus of elasticity (MOE) values of the test samples that are repeatable bent to a given deflection must be considered. In this study it was shown that sound 3mm thick ...
G Li, D Nicholas, T Schultz


Investigation on a Wood Decay Biomarker
2006 - IRG/WP 06-20336
HPLC evolution of beech wood extractives was investigated with or without exposure to Coriolus versicolor during one month. The nature and the quantity of extracts depends on the extraction conditions, but were also strongly influenced by the wood drying temperature and time leading to an important modification for higher drying temperature. After two weeks of exposure to Coriolus versicolor, resu...
S Mounguengui, S Dumarçay, P Gérardin


The Use of Modulus of Elasticity and Modulus of Rupture to Assess Wood Decay in Laboratory Soil-Bed Test
2006 - IRG/WP 06-20338
The efficacy of wood preservatives were determined in a soil-bed test. Samples of alder wood sapwood (Alnus glutinosa subsp. barbata) (5x10x100 mm) were treated with Solutions of CCA (1 % and 2 %), ACQ-1900 (2 % and 3 %), ACQ-2200 (1 % and 2 %), Tanalith E 3491 (2 % and 2.8 %), Wolmanit CX-8 (% 1 and 2 %). Modulus of Elasticity, modulus of rupture, mass loss and decay rate according to AWPA E7 wer...
A Temiz, Ü C Yildiz


Laboratory methods for assessing the resistance of wood plastic composites to fungal attack.
2006 - IRG/WP 06-20340
Wood plastic composites (WPC’s) have many attractive material features including dimensional stability and resistance to moisture, but the wood in these materials remains susceptible to fungal attack. Assessing WPC decay resistance in laboratory trials has proven difficult because the slow moisture sorption characteristics of this material do not allow for sufficient fungal attack over the trad...
J J Morrell


Testing durability of treated wood according to EN 252. Interpretation of data from Nordic test fields
2006 - IRG/WP 06-20341
To evaluate the effectiveness of new wood preservatives intended for treated wood in ground use there is a European standard, EN 252. The test shall run for five years before any formal interpretation of the results can be made. Due to environmental concerns, the use of wood preservatives based on copper, chromium and arsenic (CCA) has been restricted in recent years. As a consequence of restrict...
M L Edlund, F G Evans, K Henriksen, T Nilsson


Measuring Soil Respiration as a New Approach to Describe the Interaction Between Wood (Treated and Untreated) and Microbiologically Active Soil
2006 - IRG/WP 06-20344
Soil respirometry was employed to examine the relationship between both the performance of copper organic preservatives in contact with and the amount of carbon dioxide evolved from soil and the effect of wood treated with preservatives on carbon turnover in soil. Wood was treated with a range of copper organic preservatives intended for use in ground contact as well as an organic preservative in...
I Stephan, W Mierke


Overview of European discussions on Standardisation and list of proposed standards for WPC performance qualification
2006 - IRG/WP 06-20345
The standardisation is a driving force to promote the development of Wood Polymer Composites (WPC) by giving confidence to users and consumers. In Europe, the high interest of WPC require at this time a diffusion of information concerning the performances of WPC products. The WPC performances must be assessed according relevant standards in order to qualify the intrinsic properties of WPC (mechani...
G Labat, M Vernois, T Gay


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