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Glutathione transferases and wood extracts
2015 - IRG/WP 15-10845
Wood rotting fungi have developed an efficient detoxification system allowing them to be well adapted to their way of life, these fungi generating and encountering potential toxic compounds during wood degradation. Belonging to this detoxification network, glutathione transferases are particularly extended in these fungi and their presence could reflect the chemical environments encountered by the...
A Deroy, F Saiag, Z Kebbi-Benkeder, N Touahri, M Morel-Rouhier, F Colin, S Dumarcay, P Gérardin,


Mould growth on spruce claddings and the effect of selected influencing factors after 4 years of outdoor testing
2015 - IRG/WP 15-30671
Norway spruce (Picea abies) is the most frequently chosen wood species as claddings in façades in Norway, and is used both coated and uncoated. The visual changes that occur during weathering can be colour changes, abrasion or wear, blistering, flaking, and even cracks in the wood or coating, but more often growth of mould and blue stain fungi is the main challenge. A field test with synchronous monitoring of relative humidity, air temperature, material temperature and wood moisture content in Norway spruce claddings has been performed in southern Norway as part of the ClimateLife project. Visual evaluation of blue stain and mould growth according to EN 927-3 was performed, and evaluation data after 46 months exposure is presented. The objectives were to study the effect of 1) environment, 2) cardinal direction and 3) colour of the coated surface on growth of blue stain and mould fungi and to study the variation in relative humidity, air temperature, wood moisture content and material temperature. After 46 months exposure, the red coating system had generally the lowest mould ratings and the uncoated claddings had the highest. Of the white coloured claddings the ones facing south had lower mould ratings than those facing north, but the opposite were found for red coloured claddings. The relative humidity was higher in front of claddings exposed northwards and in a shaded environment compared to an open environment against south. In an open environment red coloured claddings had the lowest wood moisture content and highest material temperature, and red coloured claddings against south had the highest maximum material temperature.
L Ross Gobakken, G I Vestøl


Susceptibility of wood substrates to Aurebasidium pullulans at different temperatures
2016 - IRG/WP 16-10863
There is an increasing awareness of how the aesthetical performance of wood exposed outdoors changes over time and especially in the first few years after installation. Mould and blue stain fungi are biological agents that contribute to the weather grey colour on a wooden façade, and the blue stain fungi Aureobasidium pullulans is commonly identified as colonizer on coated and uncoated wood expos...
L Ross Gobakken, G Alfredsen


Identification of the key factors influencing development of the blue stain fungi
2016 - IRG/WP 16-10872
Service life of wood is more and more frequently affected by aesthetic requirements than functional ones. In order to elucidate various aspects of service life, model house with façade elements made of 22 materials was built on the field test site at Department of Wood Science and Technology in Ljubljana. Colour and fungal disfigurement is among other parameters monitored on this house. In parall...
D Kržišnik, B Lesar, N Thaler, M Humar


Effect of the Storage Time of Logs in Anti-Stain Treatment Effectiveness
2016 - IRG/WP 16-30683
Storage of green lumber, for 7 months, operationally treated with anti-stain products, depending on the storage time of the logs (10, 20 and 30 days) post harvesting allows to affirm that with increasing storage time of the logs, occurs a progressive increase in intensity, severity and types of fungi present in the logs. It is further noted, a significant increase in the percentage of sawn lumber ...
M A Peredo, C Barria


Assessment of the biological durability of wood treated with organosilicon compounds
2016 - IRG/WP 16-30685
The European wood construction market is in need of environmentally friendly wood-based products whose service life meets the expectations of end users. Non-biocidal silicon-based water repellents, which can be applied to different materials, help minimize their vulnerability/susceptibility to liquid water. Consequently, they have the potential of increasing wood’s resistance against decay fungi...
M Kutnik, M Montibus, S Derocker, S Salivati, J-P Lecomte


Resistance of heat treated Ash wood under steam pressure: rot fungi, soil micro-organisms and termites
2016 - IRG/WP 16-40724
Thermal modification processes have been developed to increase the biological durability and dimensional stability of wood. The aim of this paper was to study the influence of ThermoWood® treatment intensity on wood decay resistance improvement against soil-inhabiting micro-organisms, brown/white rots and termite’s exposures. All of the tests were carried out in the laboratory with two differen...
K Candelier, S Hannouz, M-F Thévenon, D Guibal, P Gérardin, M Pétrissans, R Collet


Colorant Treatments to Enhance the Weathering Resistance of Western Red cedar
2016 - IRG/WP 16-40737
Western redcedar is renowned for its decay resistance, but, like most wood products, it requires frequent maintenance in many exterior applications to maintain its appearance. To provide adequate long-term performance in exterior applications, it must be protected against light, water, and disfiguring fungal growth. Colorant treatments, with and without preservatives, were investigated for their a...
R Stirling, V Landry


Development of methodologies to evaluate tanning blocking coatings
2016 - IRG/WP 16-40760
In Europe an increased interest in using home-grown hardwoods as sustainable and renewable construction materials started a few years ago. Amongst these species oak and chestnut are two interesting candidates as their heartwood contain a significant amount of tannins which contribute to their natural durability. To avoid wood greying and dimensional variations, oak and chestnut must be protected b...
C Reynaud, L Podgorski


Effects of climatic factors and material properties on mould growth on untreated wooden claddings
2017 - IRG/WP 17-10884
Mould growth is an important contributor to colour change of untreated wood exposed outdoors. Predicting the development of mould growth is therefore important to ensure successful use of untreated wood as a façade material. More knowledge about the factors affecting mould growth on outdoor exposed wood is required to give better predictions. In this study, climatic factors and material propertie...
S Karlsen Lie, G I Vestøl, O Høibø, L Ross Gobakken


Fungal growth on coated wood exposed outdoors: influence of coating pigmentation, cardinal direction and inclination of wood surfaces
2017 - IRG/WP 17-10896
The objective of the SERVOWOOD project was to develop and establish European Standards that will facilitate the prediction of service life for exterior wood coatings. One of the objective of this project was to study fungal growth of the field exposed panels. Two coatings applied in 2 and 3 coats were exposed for one year outdoors at 45° south: one solventborne (alkyd based) and one waterborne (a...
L Podgorski, C Reynaud, M Montibus


Bio-friendly wood protection systems - resistance to mould and blue-stain fungi
2017 - IRG/WP 17-30713
With stricter restrictions on the use of biocides and increasing environmental awareness there is a pronounced need for effective bio-friendly protection systems in the wood preservative and wood coating industry. One of the possible pathways is to exploit and utilize natural substances with active biological effects. Silanes, caffeine, natural oils and potassium carbonate were chosen as compounds...
L Ross Gobakken, W Perdoch, B Mazela, P Kwaśniewska-Sip, G Cofta


Agar block durability test of plywood made of neem (Azadiracta indica) leaves treated veneer using two white rot fungi
2017 - IRG/WP 17-40782
This study has been undertaken to investigate the durability of plywood made from non-durable wood (simul, Bombax ceiba) veneer treated by neem (Azadiracta indica, A. Juss.) leaves. The veneers were treated with cold and hot water solution of neem leaves of different concentrations (10%, 5%, 2.5%) and treatment durations (1, 2, 3 days) for cold water and 20, 40 and 60 minutes for hot water. The pl...
K Akhter, M A Hashem, S Akhter


Black-stain Resistant Acrylic Latexes for Wood Coatings Applications
2017 - IRG/WP 17-40786
Translucent coatings on wood in exterior applications often fail due to photodegradation and colonization by black-stain fungi and require frequent refinishing. This limits competitiveness with alternative materials. In this project, it was hypothesized that an effective dispersion of inorganic nanoparticles will provide a translucent coating with long-term resistance to black-stain fungi. Functio...
G Boivin, A M Ritcey, P I Morris, V Landry


Bacterial adhesion to selected wood finishes
2017 - IRG/WP 17-40793
A new generation of furniture, including antibacterial surfaces, should adjust to the changing needs of an ageing population. Respectively, determination of bacterial adhesion on wooden surfaces, finished with various wood finishes or coating is an important topic. The main aims of our work were of a preliminary character. We wanted to state if the existing methods for testing of bacterial adhesio...
M Petrič, D Kekec, J Žigon, K Godič Torkar, K Bohinc


Plasma-assisted bleaching of blue-stain from lodgepole pine wood
2017 - IRG/WP 17-40799
We hypothesize that plasma will etch wood and fungal hyphae in blue-stained lodgepole pine, and increase the ability of hypochlorite bleach to remove discolouration from blue-stained wood. Blue-stained lodgepole pine wood was exposed to a glow-discharge plasma derived from water, and the surface wettability, permeability and colour of the treated wood was measured. Plasma-treated wood was dipped i...
A Jamali, P D Evans


Biodegradation of creosote by white rot fungus Phanerochaete chrysosporium and a strains of Pseudomonas
2017 - IRG/WP 17-50329
The aim of this study was to determine degradation effectiveness of polycyclic aromatic hydrocarbons (PAH) contained in creosote by white rot fungus and bacteria. The following two species of bacteria Pseudomonas putida and Pseudomonas sp. as well as Phanerochaete chrysosporium fungus characterised by the ability to decompose aromatic compounds were selected for experiments. Bacterial strains were...
J Zabielska-Matejuk, A Stangierska, A Kropacz, E Kaczorek


Effects of exposure time on detection of the behavior of white-rot fungi on Oriental beech wood properties
2017 - IRG/WP 17-10902
The comparison of fungal degradation of Orientalis beechwood by two white-rot fungi was evaluated in this study. Biological, mechanical, and chemical assessments of beech wood were carried out at 30 day interval for 120 days. The results demonstrated that both fungi have greater effects on beech wood during each exposure time. However, Trametes versicolor degraded beech wood more effectively than ...
E Bari, D D Nicholas, K M Ohno


Interactions between wood polyphenols and detoxification enzymes of the white rot Trametes versicolor
2018 - IRG/WP 18-10906
Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases (GSTs) is especially expanded in lignolytic fungi, little is known about their physiological target molecules. In this study, by combining thermal shift assay and affinity crystallography we highlighted inter...
M Schwartz, T Perrot, E Aubert, S Dumarçay, F Favier, P Gérardin, M Morel-Rouhier, G Mulliert, F Saiag, C Didierjean, E Gelhaye


Preliminary Investigation into the Natural Decay Resistance of Nigerian Grown Hevea brasiliensis and Mitragyna ciliata wood to Phanerochaete chrysosporium White-Rot Fungus
2018 - IRG/WP 18-10919
Wood is an important and versatile structural building material that finds applications in numerous uses. However, wood is also a biological material vulnerable to degradation by microbial activities; this is especially true in the tropics. Following the scarcity of highly durable species from our natural forests and the introduction of so many lesser used/durable wood species into the booming tim...
J M Owoyemi, U O Emmanuel


Mechanism of nano metal fluorides against fungi
2018 - IRG/WP 18-30730
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredient in wood preservatives such as copper and boron. Wood specimens treated with fluoride sols of MgF2 and CaF2 have been tested against brown-rot fungi (Coniophora puteana and Rhodonia placenta) in accordance with EN 113 and against termites (...
S M Usmani, K Klutzny, I Stephan, T Hübert, E Kemnitz


Visual appearance of unpainted wooden claddings during the first year of outdoor exposure: Evaluation of surface mould growth, lightness (L*) and wasp attack
2018 - IRG/WP 18-40841
This study addresses changes in visual appearance of unpainted wood materials exposed outdoors. Specimens of Norway spruce (Picea abies) Scots pine (Pinus sylvestris), aspen (Populus tremula), acetylated Radiata pine (Pinus radiata) and DMDHEU-modified Scots pine sapwood were exposed facing south in Ås, Norway for 60 weeks. During this period, surface mould growth development and wasp attack were...
S Karlsen Lie, L Ross Gobakken, G I Vestøl, O Høibø


The biodegradation of creosote oil by various species of white rot fungi and bacteria isolated from the contaminated soil
2018 - IRG/WP 18-50334
The aim of the study was to determine biodegradation effectiveness of the selected polycyclic aromatic hydrocarbons (PAHs) contained in creosote oil, by two white rot fungal species (Bjerkandera adusta and Irpex lacteus) and three bacterial species (Pseudomonas putida, Pseudomonas sp. OS4 and Rahnella aquatilis). Bacteria were isolated from the soil contaminated with petroleum hydrocarbons from th...
M Sydow, J Zabielska-Matejuk, E Kaczorek, AStangierska, A Kropacz


Difference of wood decay manner between brown-rot species
2019 - IRG/WP 19-10938
Wood blocks (Cryptomeria japonica) which were decayed by a Polyporales white-rot fungus (Trametes versicolor) and brown-rot fungi (Fomitopsis palustris, F. pinicola, and Wolfiporia cocos), respectively, were analyzed by X-ray diffraction and infrared spectroscopic methods followed by multivariate analysis. In the analyses, the differences in the cellulose crystallinities and infrared spectral patt...
R Kondo, Yo Horikawa, S Nakaba, K Ando, M Yoshida


From fungal detoxification systems to wood durability in neotropical forests
2019 - IRG/WP 19-10946
White-rot fungi are able to mineralize all the wood components due in particular to an efficient detoxification system. We hypothesised that components of this detoxification systems, glutathione transferases, could be used as tools to explore the natural durability of neotropical wood species. Analysis of the interactions between six glutathione transferases of Trametes versicolor and extracts of...
T Perrot, G Salzet, N Amusant, J Duchene, E Gelhaye


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