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A new type of biocide suitable for use in different fields of wood preservation
1993 - IRG/WP 93-30014
A new type of polymeric biocides was developed containing quaternary ammonium and boron ester groups as active structure elements, to overcome the disadvantages of AAC's in wood preservation use and to utilise their good performance due to a broad efficacy against fungi on the other hand. The new biocides called polymeric betains have been tested in laboratory and field tests and especial...
V Barth, H Härtner


The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


Studies and experiences of occurrence and development of soft rot in salt-treated poles of pine (Pinus sylvestris) installed in Swedish transmission lines in the years 1940-1945
1977 - IRG/WP 277
The present work describes the result of microscopic examinations of some 2,000 borings extracted from 20 to 23-year-old salt-treated poles (Scots pine) from the southern and central parts of Sweden. The purpose was to map out the occurrence of soft rot in Sweden and its influence on the mechanical strength of the poles as well as to contribute to the development of new and more satisfactory metho...
H Friis-Hansen


Fungal decay resistance of Rubber wood treated with heartwood extract of Rosewood
2005 - IRG/WP 05-30367
Alcoholic extract of Dalbergia latifolia heartwood was studied for its toxicity towards wood decaying fungi. Rubber wood blocks were treated with this extract to three different retention levels (0.1%, 0.2% & 0.5%) and the treated wood blocks were assessed for their resistance towards two white rot and two brown rot fungi. Treated blocks showed improved resistance over the control blocks. At 0...
A K Sethy, H C Nagaveni, S Mohan, K T Chandrashekar


Investigations on testing with soft rot fungi by the vermiculite-burial method
1972 - IRG/WP 215
With the object of developing a test method to determine the effectiveness of wood preservatives against soft rot attack, tests have been carried out with pure fungal cultures using vermiculite as the substrate for untreated and preservative impregnated wood blocks. In these tests the suitability of common commercial, simple disposable beakers was proved successful as a substitute for the glass bo...
W Kerner-Gang, M Gersonde


A microscopic study on the effect of IPBC/DDAC on growth morphology of the sapstaining fungus Ophiostoma piceae
1997 - IRG/WP 97-10191
An exploratory study was performed to probe into fungal/wood/fungicide interactions using Ophiostoma piceae, a common sapstaining fungus on unseasoned radiate pine. Treated (1% solution containing IPBC (0.07% w/w) and DDAC (0.60% w/w)) and untreated radiate pine wafers were inoculated with O. piceae and examined microscopically over a ten days incubation time. The results of the study showed that ...
Ying Xiao, B Kreber


Soft rot tests with soils of different origins
1984 - IRG/WP 2226
In the BAM a comparative soft rot test with 9 different types of soil (one soil each from Canada, New Zealand and Switzerland and 6 soils from laboratories in Germany) were carried out. The pH values of the different soils ranged from 3.2 to 7.1, and the amounts of organic matter were between 4 and 44% (w/w). The test specimens - Pine sapwood blocks treated with different concentrations of a CCA p...
M Gersonde, W Kerner


Accidental mold/termite testing of high density fiberboard (HDF) treated with borates and N’N-naphthaloylhydroxylamine (NHA)
2003 - IRG/WP 03-10462
High density fibreboard (HDF) was made from beech and pine furnish (50:50) and treated with boric acid (0.1-3%), borax (0.1-3%) or N'-N-(1,8-naphthalyl) hydroxylamine (NHA) (0.1-1%) prior to gluing with urea formaldehyde (UF) resin in order to determine resistance to Eastern subterranean termites (Reticulitermes flavipes Kollar), the most economically important termite species in North Am...
S N Kartal, H H Burdsall Jr, F Green III


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


An unusual soft-rot decay pattern caused by the Ascomycete Hypoxylon mediterraneum (de Not.) J Miller
1984 - IRG/WP 1222
A distinct pattern of soft-rot decay has been observed for the fungus Hypoxylan mediterraneum (de Not.) J. Miller. This fungus also produced decay patterns typical of brown and white rot decay. The production of characteristic cavities by Hypoxylan mediterraneum was prolific in the hardwoods Eucalyptus maculata and Eucalyptus regnans, but infrequent in the softwoods, Pinus elliottii and Pinus radi...
D M Francis, L E Leightley


Fungus cellar and antisapstain field trial studies of six triazole fungicides
1995 - IRG/WP 95-30077
The efficacy of six triazole fungicides was compared using a fungus cellar soil bed test, a rapid antisapstain laboratory trial and a 36 week antisapstain field trial. After 21 months in the fungus cellar the mean soundness of radiata pine treated with 1.5 kg/m³ of cyproconazole, azaconazole, hexaconazole, tebuconazole, propiconazole and flusilazole in combination with 1.0 kg/m³ didecydimethyl a...
R N Wakeling, J G Van der Waals, R D Narayan, J B Foster, B E Patterson, P N Maynard


Soft rot test of copper/chrome/arsenic treated heartwood of three Malaysian timbers by the vermiculite-burial method. (+ correction document of 25 July 1990)
1990 - IRG/WP 2354
Heartwood of copper-chrome-arsenic (CCA) treated kempas (Koompassia malaccensis), tualang (Koompassia excelsa) and keruing (Dipterocarpus spec.) was found to be susceptible to soft rot in recent pole surveys. Standardized heartwood blocks were impregnated with 0 to 6.3% (w/v) CCA and challenged to decay for twelve weeks by a mixed inocula of Chaetomium globosum, Glenospora graphii, Humicula grisea...
R-D Peek, A H H Wong


The effect of copper/chrome/arsenic (CCA) treated timber on soil fungi
1981 - IRG/WP 1131
The aim of this investigation was to study the effect of CCA treated wood on the microflora of soil with particular reference to copper tolerant soft rot fungi. The IRG collaborative field experiment was designed to monitor the performance of a range of preservative treated hardwoods with particular reference to soft rot in CCA treated timber (Dickinson 1976) and site 33 of this experiment was use...
R J Murphy, D J Dickinson


Objectives of future collaborative soft rot tests
1972 - IRG/WP 209
Progress has been made towards acceptance of a defined laboratory test method for determining the toxicity of preservatives against soft rot fungi (Documents IRG/WP/201 and IRG/WP/208) and, at the 1971 meeting in Brussels, the framework of collaboration for the next phase of testing was discussed. Time available at Brussels did not permit restatement of objectives, consideration of the stages by w...
J G Savory


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Evaluation of a laboratory soft rot test on basis of weight loss and residual strength
1989 - IRG/WP 2336
Based on the principles of different previously reported experimental procedures dealing with laboratory soft rot testing, a study was conducted on treated poplar and beech. Preservatives used were CCA, CCF, AAC, Azaconazole and TCMTB. Treated samples were exposed for three months in a vermiculite substrate to Chaetomium globosum. Evaluation was based on absolute mass loss, percentage weight loss ...
W Verbeke, J Van Acker, M Stevens


The development of soft rot decay in bamboo fibres
1992 - IRG/WP 92-1572
The development of decay by soft rot fungus Chaetomium globosum was investigated in young and mature culms of bamboo Phyllostachys virideglaucescens. Soft rot decay was influenced both quantitatively and qualitatively by the differing levels of lignification in the material. Weight loss was significantly greater in the younger culm compared with the older, more heavily lignified culm. The distribu...
O Sulaiman, R J Murphy


Potential toxicants for controlling soft rot in preservative treated hardwoods. Part 2: Laboratory screening tests using sawdust
1977 - IRG/WP 2101
A technique is described for the rapid screening of toxicants against microorganisms, represented by the brown rot, Gloeophyllum trabeum, and a natural inoculum from soft rotted wood. Sawdust, in this case from sapwood of Eucalyptus maculata, is pretreated with the candidate formulation and moistened before inoculation. Visual scoring of growth can be used to discriminate suitable candidates as ea...
J D Thornton


Production of monoclonal antibodies to Serpula lacrymans and their application in immunodetection systems
1993 - IRG/WP 93-10004
Monoclonal antibodies were produced against both whole cell mycelial extracts and exo-antigen extracts of the causative organism of dry rot, Serpula lacrymans. The antibodies were tested for cross-reactivity against fifteen strains of Serpula lacrymans and nine other fungal species. These species represented either other wood decay fungi or non-decay fungi commonly co-isolated with Serpula lacryma...
H Glancy, J W Palfreyman


The effect of stilbenes on decay fungi of birch and aspen
2000 - IRG/WP 00-10364
Fourteen species of decay fungi, mainly species growing on birch and aspen, one leaf spot fungus of birch and the pine canker fungus Gremmeniella abietina were used to investigate how the stilbenes resveratrol and pinosylvin influence their relative growth on malt agar. With some species the ability to decay with stilbenes impregnated wood was also studied. The concentration of pinosylvin needed t...
L Syrjälä, K Von Weissenberg, A Pappinen, L Paajanen


Preliminary investigation on the natural durability of Guayule (Parthenium argentatum)-based wood products
2000 - IRG/WP 00-40154
Conventional preservatives used to protect wood from insect and microbial damages are presently of major concern to human health and the environment. Finding alternative and economical preservatives has not been successful. Previous studies have shown that the resinous material extracted from the guayule plant (Parthenium argentatum, Gray) has both insect- and microbial-resistant properties. Unfor...
F S Nakayama, P Chow, D S Bajwa, J A Youngquist, J H Muehl, A M Krzysik


Studies in an accelerated soil bed facility on the decay susceptibility of U.K. grown spruce and pine poles treated with copper/chrome/arsenic (CCA) by pressurised sap-displacement. Part 1: Setting up of soil beds and initial soft rot results
1990 - IRG/WP 2344
The paper describes the methodology used in the construction and early operation of an accelerated soil bed facility used to examine the decay susceptibility of U.K. grown Scots and Corsican pine and Sitka and Norway spruce treated with C.C.A. by high pressure sap-displacememt. The design and control of the facility as well as the preparation, soil exposure and soft rot decay analysis of quarter p...
A Bruce, S D Hainey, G M Smith, B King, P D Evans


Soft-rot testing. Memo to CEN
1983 - IRG/WP 2206 A
1. Development of a single test procedure to assess performance of preservatives against soft rot fungi is an ideal that cannot be realised at present, if results are to be both reproducible between laboratories and pertinent to the practical requirements of individual countries. 2. Consensus opinion amongst members of Sub Group I - Soft rot tests, of Working Group II - Fundamentals of Testing, of the IRG, is that the immediate needs of CEN would be best answered by: (a) a code of recommended practice, (b) a multiplicity of test procedures, and (c) a degree of flexibility in test procedures. 3. The code of recommended practice should recognise the need for: (a) a pure culture, synthetic medium (sand or vermiculite + nutrients), single wood species test to be adopted by all laboratories as a common reference point of testing, (b) mixed inoculum and/or unsterile soil laboratory or fungus cellar tests which account for local variations in the fungal flora, (c) incorporation of a range of wood species where applicable (i.e. where mixed hardwoods comprise the main resource), (d) provision of local internal standards of known performance under field conditions, (e) incorporation of leaching procedures other than standard methods with deionized water (e.g. mild acid, milk alkali, ionic solutions of various salts) where applicable to local exposure conditions, (f) precise definition of the criteria used for establishing toxic thresholds (mass loss, strength loss, microscopic examination). 4. Initial screening tests should remain the province of the individual research worker. 5. The code of recommended practice should have the sole purpose of establishing provisional retention levels for subsequent confirmation by field trial. 6. IRG should list and approve test procedures considered adequate to establish toxic thresholds for preservatives against soft rot fungi. 7. It should be clearly understood that when using a series of test techniques, it is inevitable that a range of toxic thresholds will be obtained for the same preservative. 8. Guidelines should be drawn up for interpretation of test data for subsequent transformation to provisional retention levels. 9. Adoption of the broad principles outlines above is recognition of the dynamic nature of toxic thresholds. The additional information obtained by a more flexible, and complex, approach to testing may make interpretation more difficult but should provide data from which response of preservatives to varying biological and environmental hazards can be more realistically assessed. 10. It is recommended that CEN adopt, in principle, the basic philosophy of testing outlined above and move towards formulation of a Working Document in collaboration with IRG
J A Butcher, D J Dickinson


List of fungi in soft-rot tests
1975 - IRG/WP 105
This list gives information on the terrestrial and marine species and strains of fungi tested for soft-rot ability in pure culture with data on their decay activity in untreated wood. An index of the timber species tested is added. The compilation is based on 1) the list of soft-rot fungi of the former OECD Group (Doc.No. 27/DAS/CSI/M/559), Oct. 1968, prepared by R. Rösch and W. Liese, 2) informa...
G Seehann, W Liese, B Kess


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