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Protection of wood for above ground application through modification with a fatty acid modified N-methylol/paraffin formulation
2007 - IRG/WP 07-40378
Hydrophobic chemical modification can be regarded as an alternative approach to conventional wood preservation using biocides in order to protect wood in hazard class 3 according the European Standard EN 335-1. A formulation containing fatty acid modified N-methylol/paraffin and an aluminium salt catalyst was used to change the material properties of wood in order to protect wood from fungal dec...
Nguyen Hong Minh, H Militz, C Mai


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


Detection of Anti-Fungal Sapwood Extractives in Non-Durable Scots Pine (Pinus sylvestris), Rubberwood (Hevea brasiliensis) and Jelutong (Dyera costulata)
2007 - IRG/WP 07-10634
A general laboratory bioassay method of Woodward and Pearce (1985) was adopted to detect anti-fungal activity of sapwood or heartwood extractives of 5 Malaysian hardwoods [dark red meranti heartwood (Shorea spp.), red balau heartwood (Shorea spp.), kulim heartwood (Scorodocarpus borneensis), jelutong sapwood (Dyera costulata) and rubberwood sapwood (Hevea brasiliensis), including the temperate Pin...
A H H Wong, R B Pearce


Characterization of protein patterns from decayed wood of loblolly pine (Pinus taeda L.) by proteomic analysis
2008 - IRG/WP 08-10654
The primary biotic decomposers of wood belong to the basidiomycetes. The members of this group can attack and biodegrade both wood in the forest and in service. By the time wood decay is visible, there has already been a significant loss of strength. The identification of basidiomycetes and other organisms on wood only tell us what is present, not what is actively decaying the wood. When organisms...
Young-Min Kang, L Prewitt, S Diehl


Soft Rot Determines Service Life of L-joints with Low Borate Loading
2008 - IRG/WP 08-30470
As part of a research program on the potential use of borates by the Canadian wood products industry, Forintek set up an L-joint test of untreated and borate-diffusion treated hem-fir (western hemlock and amabilis fir) in 1990. The treated material had a low initial retention of only 0.2% boric acid equivalent and analysis after 5 years revealed no detectable borate close to the tenon. Nev...
P I Morris, J Wang, J K Ingram


Resistance against basidiomycetes of 1,3-dimethylol-4,5-dihydroxy ethylene urea (DMDHEU)-modified plywood of Pinus sylvestris
2008 - IRG/WP 08-40398
Plywood produced with rotary-cut veneers of Pinus sylvestris impregnated with a solution of 1.3 M 1.3-dimethylol-4.5-dihydroxyethyleneurea (DMDHEU) and glued with a phenolic resin (Prefere 4976 DYNEA) were inoculated with Coniophora puteana, Coriolus versicolor and Pleurotus ostreatus, following the European standard ENV 12038. The specimens were leached following European standard EN 84. The re...
A Dieste, A Pfeffer, S Bollmus, H Militz


Effect of a biological treatment on below ground decay of Douglas-fir pole sections
2008 - IRG/WP 08-40433
The use of exogenous sugars to accelerate microbial growth and eventually limit available oxygen in soil surrounding wood in soil contact was investigated on untreated Douglas-fir poles exposed over a 3 year period in Western Oregon. Isolation frequencies differed markedly between treated and untreated poles and the treatment did appear to shift the frequency of some basidiomycetes. The treatment ...
C Freitag and J J Morrell


Effect of different ASAs (alkenyl succinic anhydrides) on the treatment of biological protection of wood in use class 4
2008 - IRG/WP 08-40440
The alkenyl suscinic anhydrides (ASAs) used in this work are adducts resulting from the maleinization reaction of fatty acid alkyl esters. Various products with different alkyl groups were synthesized from rapeseed (Brassica napus) oil esters, rich in oleic acid. The liquids obtained showed a viscosity similar to that of vegetable oils and a brown color. When applied into wood, a thermal treatment...
C Vaca-Garcia, O Pignolet


Serpula lacrymans – calcium, iron, and foundering wooden boats
2009 - IRG/WP 09-10691
Serpula lacrymans is one of the most destructive wood-degrading brown rot fungi in temperate environments. Its virulence has often been linked to its ability to grow over non-woody materials and extract calcium (Ca) or iron (Fe) to promote wood degradation in buildings. This fungus has also been a severe problem in historic wooden warships and in modern wooden vessels, sometimes leading to founder...
J S Schilling, S M Duncan


Gene expression of selected decay enzymes produced during biodeterioration of three wood types
2009 - IRG/WP 09-10702
Comparative studies were conducted on the expression of several decay enzyme genes during the decay of pine, cedar, and ACQ treated pine over 10 months. Measurements of MOE, decay rating, and moisture control were monitored for physical properties. Identification of decay fungi and the detection of decay enzymes were carried out before gene expression levels were measured. The MOE of wood stakes d...
Young-Min Kang, L Prewitt, S Diehl


Effects of cold treatment on wood destroying fungi important in cultural heritage
2009 - IRG/WP 09-10706
The dry rot fungus Serpula lacrymans (Wulfen:Fr.) Schroeter is notorious in building environments for its vitality, destructive potential, and the huge costs associated with remediation of damage caused to wood of cultural heritage. Due to the potentially adverse of effects of chemical treatment methods such as have been attempted in the past, more benign physical methods of treatment, e.g. heat t...
M Lüdicke, W Unger, G Binker


Degradation of wood veneers by Fenton’s reagents: effects of low molecular weight phenolic compounds on hydrogen peroxide decay and tensile strength loss
2009 - IRG/WP 09-20400
Pine wood (Pinus sylvestris) veneers strips were incubated in acetate buffer containing hydrogen peroxide and iron to mimic mechanisms of brown rot decay and assess the degradation of cellulose through analysis of the tensile properties of the decayed wood. The tensile properties of thin wood strips treated with Fenton system reagents or precursors were determined and correlated to weight loss as ...
Yanjun Xie, R Well, Zefang Xiao, B Goodell, J Jellison, H Militz, C Mai


Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal


Wart Morphology can Distinguish White Cypress Pine from the Less Durable Species, Black Cypress Pine
2009 - IRG/WP 09-20406
White cypress pine (Callitris glaucophylla) wood is durable enough to be used outdoors, but occasionally there are reports of its premature failure in ground contact, which may be due to its substitution by the less durable species, black cypress pine (C. endlicheri). It has been difficult to prove this, however, because the woods of both species are very similar in structure and cannot be separat...
R Heady, R Cunningham, P Evans


Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima


The resistance of thermo-oil modified wood against decay and mould fungi
2009 - IRG/WP 09-40448
Thermally modified wood in comparison to natural wood demonstrates some advantageous functional properties like decreased hygroscopicity, higher dimensional stability. It also has increased durability and improved resistance to fungal decay. However thermal modification of wood may lead to reduced strength properties what may limit the use of wood in mechanically beared constructions. The changes ...
A Fojutowski, A Kropacz, A Noskowiak


A genomic sequencing approach to study wood decay and copper tolerance in the brown rot fungus, Antrodia radiculosa
2010 - IRG/WP 10-10720
We used Illumina paired-end short read sequencing (76 nt, 300 bp insert size) to produce a de novo assembly of the genome of Antrodia radiculosa, a copper-tolerant brown rot fungus that is capable of aggressive wood decay. Quality analysis of the base calls in the dataset (8.95 Gb) showed that the majority of the nucleotide sequence was of the highest quality with 5% in the lowest quality group. ...
J D Tang, T Sonstegard, S Burgess, S V Diehl


Structural changes, basidiomycete richness, enzyme activity and proteomic profiling of decay resistant and non-resistant woods over 18 months in soil contact
2010 - IRG/WP 10-10733
Wood decay fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to better understand which suite of decay genes and proteins are expressed during biodeterioration of three different wood types in forest soil over time. Variation in decay genes and proteins were determined for pine (non-resistant), cedar (naturally durable), and ACQ-treat...
Youngmin Kang, S Diehl, L Prewitt, D Nicholas


The brown rot of oak wood submerged for 6 months in the Baltic sea
2010 - IRG/WP 10-10736
The natural wood submerged or partially immersed in sea water is commonly affected by certain microorganisms. The bacteria and marine borers that decay the wood totally submerged in sea water and above sea level other microorganisms as fungi, algae, insects may attacked wood. The sea water may affect and change the properties of wood. We have tried to determine the susceptibility to decay caused b...
A Fojutowski, A Kropacz, I Pomian


Natural durability of African Padauk and Niove. Comparison of new trials and former data from the CIRAD database
2010 - IRG/WP 10-10743
African Padauk (Pterocarpus soyauxii) and Niove (Staudia kamerunensis) were tested in laboratory against Basidiomycetes. The results were calculated according to the requirements of EN 350-1 (1994) and XP CEN/TS 15083-1 (2006). Padauk samples showed a high durability and very small variations between trees and within tree. Niove presented a high durability, but with notable differences according ...
Y N N’Kolo Meze’e, N Leménager, C Baudassé, M-F Thévenon


Measurements of rot fungal activity as a function of moisture content by isothermal calorimetry
2010 - IRG/WP 10-20428
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. I...
L Wadsö, A Pilgård, G Alfredsen


A Comparison of the Performance of Related Copper Based Preservatives against Soft Rot
2010 - IRG/WP 10-30540
The performance of pine and beech wood treated with either a soluble copper + quat (ACQ type D) preservative system or a particulate copper + quat system was evaluated in unsterile soil using the European standard ENV 807 soft rot decay test procedure. In addition, to compare soft rot performance of soluble and particulate copper directly without the influence of co-biocides, beech and pine test...
M Ray, D Dickinson, K Archer


Testing of decay resistance of sapwood and heartwood of thermally modified Scots pine and Norway spruce
2010 - IRG/WP 10-40523
Thermal modification at elevated temperatures changes the chemical, biological and physical properties of wood. One of the main targets of thermal modification is to increase the biological durability and decay resistance of wood. Although the effects of thermal modification on wood have been widely studied, the significance of sapwood and heartwood on the resistance of thermally modified wood has...
H Viitanen, S Metsä-Kortelainen


Bioresistance of Poplar Wood Compressed by Combined-Hydro-Thermo-Mechanical Wood Modification (CHTM) Process
2010 - IRG/WP 10-40532
Bioresistance of treated Poplar wood by CHTM process (Combined-Hydro-Thermo-Mechanical Wood Modification) was studied in the current research work. Resistance against brown rot fungus Gloeophyllum trabeum as well as the soft rot decay was the main concern of this work. Poplar wood blocks were hydrothermally treated at temperatures of 120,150 and 180°C for holding time of 0, 30 and 90 min. afterwa...
L Khademi-Bami, B Mohebby


Gene expression analysis of a copper-tolerant brown rot fungus on MCQ-treated wood
2011 - IRG/WP 11-10748
Most brown rot fungi are copper-tolerant, which makes them difficult to control with copper-based wood preservatives like MCQ. To better understand what biological processes are regulated, we used our model species, Antrodia radiculosa, to examine expression of genes on MCQ-treated wood. Our hypothesis was genes that decreased copper bioavailability would be up-regulated early, when wood showed ...
J D Tang, A Perkins, S V Diehl


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