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The preliminary characterization of ß-1,4-xylanase of the brown-rot fungus Gloeophyllum trabeum
1990 - IRG/WP 1447
The extracellular ß-1,4-xylanase of the brown-rot fungus, Gloeophyllum trabeum, was isolated from crude extract by chromatofocusing method (PBE 94 column chromatography). The isoelectric point was estimated to 4.2-4.8 by cromatofocusing and 4.5 by isoelectric focusing (IEF). The molecular weight of the enzyme was estimated to 37,000 dalton by SDS-PAGE. The optimal temperature for the crude extrac...
A-C Ritschkoff, M Rättö, L Viikari


Comparison of the effect of different soil sources on the type and rate of decay of CCA-treated pine exposed in a soil-bed
1984 - IRG/WP 2213
The types of decay observed in CCA-treated pine posts in horticultural situations in New Zealand can be reproduced using a soil-bed exposure. Radiata pine stakelets, untreated or treated to 1.4, 2.7, or 5.4 kg/m³ with Tanalith NCA, were exposed to six different soil sources. The local nursery soil used for all standard laboratory tests was found to represent the greatest decay hazard to untreated...
J A Drysdale


Wood degradation mechanisms by the brown rot fungus Gloeophyllum trabeum
1997 - IRG/WP 97-10229
A mechanism for the degradation of wood by the brown rot fungus Gloeophyllum trabeum is outlined. The mechanism includes the function of redox-cycling, low molecular weight phenolic derivatives which sequester and reduce iron in acidic environments. The role of oxalate for the sequestration of iron (hydr)oxides and the pH dependent transfer of iron to the G. trabeum phenolic chelators, as well as ...
B Goodell, J Jellison


Natural durability studies in an accelerated field simulator - A novel approach
1983 - IRG/WP 2197
A study of the natural durability of untreated timbers to both decay and termite attack is described. The work illustrates the versatility of the Accelerated Field Simulator as a novel approach to biodeterioration research....
G C Johnson, J D Thornton, J W Creffield, C D Howick


Biological control of blue stain on wood with Pseudomonas cepacia 6253. Laboratory and field test
1989 - IRG/WP 1380
Pseudomonas cepacia strain 6253 was tested as a biological control agent on wood samples under laboratory conditions, as well as in the field. In the laboratory this bacterium controlled blue stain on Pinus radiata, but field test were not totally satisfactory. However, after 2 months of field test, the test samples were 1/3 to 1/2 less stained then the control samples....
R Benko


Theoretical and practical experiments with eradication of the dry rot fungus by means of microwaves
1992 - IRG/WP 92-1577
Engineer Claus Andersen constructed a device in 1986 for microwave treatment of fungal infested timber. The device was tested on ampullae with live fungal mycelium of the dry rot fungus. A 10 minutes treatment at 37°C gave satisfactory eradicating effect. The method has since been used in practice in approximately 100 instances. A spot-test control has shown satisfactory results....
J Bech-Andersen, C Andersen


A summary of work carried out to compare natural and artificial weathering for preconditioning test specimens in testing anti-stain chemicals for wood in service
1976 - IRG/WP 266
Since 1972 work has been in progress within CEN to establish a European standard for the testing of wood preservatives designed to prevent blue-staining of wood in service. The method is based on the Butin test and involves pre-weathering of the samples prior to biological testing. The weathering regime employed is 6 months outside exposure from March to October. This imposes severe limitations on...
D J Dickinson, A F Bravery


Comparison of decay rates of preservative-treated stakes in field and fungus cellar tests. Results after 40 months fungal cellar exposure
1983 - IRG/WP 2200
Decay rates of preservative-treated Pinus radiata stakes during 40 months exposure in the FRI fungus cellar were compared with those of similarly treated material in a field test. Decay rates in the fungus cellar were from 4 to 100 times higher than in the field, although for the majority of preservatives the rate was between 7 and 12 times higher. The lag phase before onset of decay, noticeable w...
M E Hedley


Blue stain resistance of exterior wood coatings as a function of their typology
1998 - IRG/WP 98-20145
Paints and wood stains were evaluated on their blue stain resistance using both EN 152 method and the reverse method. The typology of these exterior coatings was varied including standard and high solid solvent-borne coatings as well as different types of waterborne coatings. For the water-borne acrylic, alkyd based and hybrid coatings both applied as opaque primer paints and as decorative wood st...
J Van Acker, M Stevens, C Brauwers, V Rijckaert, E Mol


Methyl bromide eradication of the oak wilt fungus in logs. Laboratory and field fumigation
1981 - IRG/WP 3168
Concern over accidental introduction of the oak wilt fungus (Ceratocystis fagacearum) into oak-importing nations has prompted a study supported by the U.S. National Lumber Exporter's Assn. to assess the efficacy of methyl bromide fumigation to eradicate the fungus from logs and lumber. Laboratory and field fumigation trials to develop a reliable and realistic treatment were performed on r...
E L Schmidt, M M Ruetze, D W French


Preliminary note on the fungal problem of rubber wood
1983 - IRG/WP 3246
Susceptibility of rubber wood to fungal attack limits its wider utilisation. Fungal problems encountered in treating rubber wood with boron compounds by diffusion process have been discussed. Sodium pentachlorophenoxide and 2-thiocyanomethylthio benzothioazole (TCMTB) were investigated for possible control of fungal growth during diffusion storage and their performance has been reported....
R Gnanaharan


Bacteria as possible organisms for biological control of blue stain
1988 - IRG/WP 1339
The article discusses the possibilities of biological control of blue stain. Besides using some antagonistic fungi, the possibility of using antagonistic bacteria, which offer still greater possibilities, should not be overlooked. Tests performed have shown that some bacteria from the genera Streptomyces and Pseudomonas have a strong antagonistic effect of blue stain....
R Benko


Report of activity of CEN/TC 38: Test Methods for Wood Preservatives
1987 - IRG/WP 2287
G Castan


Rapport sur l'activité du CEN/TC 38 "Méthodes d'essais des produits de préservation du bois"
1982 - IRG/WP 2188
M Pottevin


Evaluation of an alkyl ammonium compound as a fungicide to control sapstain and mould during diffusion storage
1984 - IRG/WP 3282
An alkyl ammonium compound ('Akzo' ES 255) was evaluated for its effectiveness against mould and sapstain during diffusion storage of boron-treated rubber wood. Though ES 255 at 1.0% concentration was effective against mould (71%) and sapstain (89%) it is less satisfactory compared to 0.5% sodium pentachlorophenoxide against mould (92%) and sapstain (98%)....
R Gnanaharan


Long-term protection of stored lumber against mould, stain, and specifically decay: A comparative field test of fungicidal formulations
1984 - IRG/WP 3281
The problem of decay in packaged, unseasoned lumber stored for many months has become of major importance in recent years. Large financial claims have resulted from decay in Canadian lumber stored at length in seaports and storage yards of distributors. For decades acceptable protection from moulds and sapstain was readily achieved with chlorinated phenols applied at appropriate treating levels. H...
A J Cserjesi, A Byrne, E L Johnson


The dry rot fungus Serpula lacrymans, its growth and damaging of wood
2004 - IRG/WP 04-10511
The dry rot fungus (Serpula lacrymans) is one of the most dangerous wood rotting fungi, especially in the built environment. In our mycological laboratory more experiments with this fungus have been carried out aimed at:- its growth under stable and variable climatic conditions, - its possibility to attack either natural wood of different species and also modified wood primary pre-treated with ...
L Reinprecht


Laboratory evaluation of chlorothalonil formulation for stain and mold control on rubberwood and maple
1998 - IRG/WP 98-30175
We evaluated the efficacy of several chlorothalonil and carbendazim fungicides (F1 and F2), etc. in the control of mold and stain fungi on rubberwood and maple. The results showed that these formulations effectively inhibited the selected fungal species such as Aspergillus niger, Penicillium sp., Trichoderma sp. (P71H), Aureobasidium pullulans, Ceratocystis minor (C-188), Ceratocystis pilifera (RW...
Mingliang Jiang, T L Highley, L Ferge, T L Woods


Soil-bed studies. Part 2: The efficacy of wood preservative
1983 - IRG/WP 2205
Various methods of decay assessment were investigated. Three stages or phases of decay were identified which could be used to describe the efficacy of a preservative system or virulence of a soil-bed testing medium. These included the lag, decay, and senescent phase. Premature senescence could arise if wood samples became waterlogged. It was concluded that time to failure was unsuitable as a metho...
P Vinden, J F Levy, D J Dickinson


Tolylfluanid - fungicide against blue stain in service
1992 - IRG/WP 92-3736
Physical and chemical properties and efficacy of Tolylfluanid are compared with the properties of Dichlofluanid. Whereas the difference in the molecular structure influences the physical properties significantly, the efficacy of both fungicides is comparable. The toxicological and ecotoxicological profile of Tolylfluanid is summarised....
H-U Buschhaus


Preventing fungal attack of freshly sawn lumber using cinnamon extracts
2007 - IRG/WP 07-30432
The potential for using cinnamon oil as an anti-mold and stain compound was investigated on ponderosa pine sapwood. Cinnamon oil was highly effective when used in ethanol, but its activity declined when it was mixed with only water. Attempts to enhance water solubility with surfactants improved solution stability, but had no apparent effect on biological activity. Further studies with other co-so...
Shujun Li, C Freitag, J J Morrell


Chapter 6 - Preservatives of bamboo
2007 - IRG/WP 07-10635-06
Almost all currently available oil-borne, water-borne and compound types of preservatives suitable for the preservation of bamboo or wood have been described along with their classifications, applications, formulations, merits and demerits, history of invention or discovery and development. The preservatives suitable for wood are also considered suitable for bamboo....
A K Lahiry


Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch


Biosynthesis of ß-Glucan microfibrils by cellular fractions from brown-rot fungus Postia placenta (MAD-698 and ME-20) and white-rot fungus Schizophyllum commune (MAD-619)
1993 - IRG/WP 93-10025
In this study, we compared the brown rot fungus Postia placenta (MAD-698 and ME-20) with the white rot fungus Schizophyllum commune (MAD-619) to determine the location and distribution of glucan synthetase. We also measured the soluble protein content in subcellular fractions obtained by differential centrifugation MAD-698 is a degradative isolate, but ME-20 and MAD-619 do not produce significant ...
S C Croan, T L Highley


Optimum growth conditions for the metal-tolerant wood decay fungus, Meruliporia incrassata TFFH 294
1999 - IRG/WP 99-50142
There is a worldwide need for alternative methods for the treatment and disposal of CCA-treated waste wood. Illman and Highley (IRG/WP 96-10163) reported the isolation of a unique strain of Meruliporia incrassata (TFFH 294) with tolerance to CCA. The strain is capable of degrading CCA treated waste wood, giving a 40% weight loss in the ASTM soil block test. The strain is an ideal candidate for deg...
V W Yang, B Illman


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