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Black Stain of Western red-cedar by Aureobasidium pullulans and its Relationship with Tropolone Depletion
2005 - IRG/WP 05-10564
Western red-cedar is valued for its natural durability conferred by fungicidal tropolone chemicals. However, weathered surfaces of WRC products are still susceptible to ‘black stain’ caused by fungi such as Aureobasidium pullulans. The effect of weathering on the tropolone content of heartwood was characterized and correlated with the ability of this fungal species to colonize the same weather...
R Chedgy, R Daniels, P I Morris, C Breuil


Mold and stain fungi associated with Radiata pine logs imported from New Zealand
2000 - IRG/WP 00-10346
The Korean sawmills have recently realized the importance of prevention of fungal discoloration due to increased losses in revenue. Before establishing integrated control strategies of fungal discoloration, more complete knowledge about causal organisms is needed. As a first step, we initiated a through survey of mold and stain fungi colonized commercially important softwood species (Pinus densifl...
Jae-Jin Kim, Gyu-Hyeok Kim


Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison


Blue stain in timber in service. Results of further IRG collaborative tests to compare different artificial weathering systems
1981 - IRG/WP 2146
The previous work described in Document No IRG/WP/286 examined 3 preservatives (1% phenyl mercury succinate - PMS, 5% pentachlorophenol - PCP and 1.5% Preventol A4 - PA4) subjected to 5 different artificial weathering systems (BAM Xenotest, Cuprinol Marr, EMPA Xenotest, Gori Atlas and PRL Marr) and after natural weathering at 5 sites (BAM, Cuprinol, EMPA, Gori and PRL). Results showed good correla...
A F Bravery, D J Dickinson


Susceptibility of painted wood to discolouring fungi - influence of binder, solvent and surfactant
1992 - IRG/WP 92-3714
Previous studies have shown that the basic composition of paints, without fungicide addition, can significantly contribute to the varying microbial susceptibility of painted wood. Previously, non-weathered water-borne acrylic paints applied to wood without a primer were shown to be more susceptible to mould attack than solvent-borne alkyd paints. The present study was initiated to reveal if this d...
J Bjurman, C Herder


Factors affecting decay rates in a fungus cellar II
1986 - IRG/WP 2259
Tests were initiated to investigate the influence of various factors on the decay rate in a "Fungus Cellar". Birch and pine stakes treated with chromated copper arsenate and didecyldimethyl ammronium chloride, as well as untreated control stakes, were incubated in two soils in a Fungus Cellar test and installed at two field sites for comparative purposes. The visual rating vs actual stake weight l...
P A McKaig


Comparison between two laboratory test methods for determining the effectiveness on wood preservatives against blue stain in fresh wood
1987 - IRG/WP 2289
Most of the work done on determination of the effectiveness of new formulae for treating fresh wood against blue stain have been focussed on their use in the manufacture of saw timber. This work explains two laboratory methods, one which simulates the working and climatological conditions of factories making packages for fruit and vegetables in the Spanish Levante, showing that contamination of wo...
A M Navarrete, M T De Troya


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


Evaluation of chlorothalonil for stain and mould control on lumber
1992 - IRG/WP 92-3713
Chlorothalonil is a commercially important fungicide with many industrial and agricultural applications. It has a very low mammalian toxicity and is considered to be an environmentally benign material. This paper describes the laboratory evaluation of chlorothalonil as a wood antisapstain treatment. Chlorothalonil was tested as three formulation types - an emulsifiable concentrate (EC), a water-ba...
P E Laks, T L Woods, D L Richter


Blue stain in service on wood surface coatings. Part 1: The nutritional requirements of Aureobasidium pullulans
1992 - IRG/WP 92-1556
The nutritional requirements of Aureobasidium pullulans was examined with regard to nutrient sources that are potentially available in fresh and weathered wood. The study was designed to determine how far wood cell wall components need to be broken down during weathering before they provide a useful nutrient source for Aureobasidium pullulans. Various carbon sources were tested, with eight differe...
P R Sharpe, D J Dickinson


Laboratory studies on control of sapstain and mold on unseasoned wood by bacteria
1991 - IRG/WP 1493
A malt-agar and nonagar laboratory test was used to evaluate the efficacy of bacterial preparations as biological control agents against several sapwood-inhabiting fungi. Both nonsterilized and filter sterilized bacteria preparations prevented attack by the sapwood-inhabiting fungi. Streptomyces rimosus was the most effective bacteria, giving good protection, with a 1-hr soak in a filter-sterilize...
T L Highley, R Benko, S C Croan


Patterns of decay in CCA-treated horticultural post populations - A fungus cellar simulation
1986 - IRG/WP 1286
The distribution of decayed posts in 10-, 14-, and 18-year-old vineyards supported a hypothesis to explain variability in intensity of decay found among posts in New Zealand horticultural properties. The development of decay patterns between samples in a simulated vineyard plot established in the Forest Research Institute fungus cellar gave additional support to the hypothesis that decay within la...
M E Hedley


Effectiveness of some microbiocides against the development of molds and sapstain in Pinus elliottii
1981 - IRG/WP 3169
Eleven commercial biocidal formulations were tested in the field to determine their effectiveness in protecting logs and lumber of Pinus elliottii sapwood from sap stain and molds. Among these formulations 2-(thiocyanomethylthio) benzothiazole showed the best performance in controlling both types of fungi. Potassium N-hydroxymethyl-N-methyl dithiocarbamate and the mixture potassium N-methyl dithio...
S Milano


Old and new facts on the dry rot fungus Serpula lacrymans
1991 - IRG/WP 1470
The article collates some of the recent literature on the biology of the dry rot fungus Serpula lacrymans. The fungus can grow at 28°C, and maximum wood moisture is above 55%. Serpula Iacrymans degrades crystalline cellulose. The intensive production of extracellular oxalic acid is neutralized by calcium and iron. There is considerable variation among the strains with regard to factors such as gr...
O Schmidt, U Moreth-Kebernik


An attempt to grow dry rot fungus Serpula lacrymans on mortar substrate in non-sterile conditions
1989 - IRG/WP 1385
According to many experts, Serpula lacrymans is probably the most dangerous wood destroying fungus in houses. Investigations of KOCH, A.P. (2) showed that incidence of this fungus in percent was around 20% when the frequency of some other standard fungi was far below (Coriolus versicolor: 0.2% for example). The work presented here is a part of a program carried out at CTFT for assessing the resist...
L N Trong


Toxic values derived from EN 113 tests - are they determined by the virulence of a test fungus? - Results from a round robin test -
1999 - IRG/WP 99-20176
The virulence of Coniophora puteana BAM Ebw. 15, the obligatory test fungus on softwood in tests according to EN 113, is known to be rather inconstant at least in some laboratories. The mass losses of untreated Scots pine sapwood blocks in an EN 113 test may range from below 20% to up to more than 50%. Possible reasons for these differences as well as the impact of a low virulence on the toxic val...
H Leithoff, R-D Peek, H V Borck, R Goettsche, H Kirk, M Grinda


Preservation of wood-based panels against fungi and insects and and testing its efficiency
1976 - IRG/WP 270
Wood-based panel products which are made of susceptible wood species may be destroyed by fungi under wet conditions and by termites. The glues do not provide sufficient protection unless very high concentrations are applied. Particle boards and fibre boards are not susceptible to beetle infestation, although some species may attack plywood. Various types of preservatives provide sufficient protect...
G Becker, M Gersonde


Sapstain development on Jack pine logs in Eastern Canada
2000 - IRG/WP 00-10358
During 1998-99, a study was initiated to investigate the influence of seasons, log types and storage time on the sapstain development on jack pine logs at two sites in Eastern Canada. Jack pine trees (Pinus banksiana) were harvested into whole-tree and cut-to-length logs in spring and in autumn. Sapstain development was examined in these logs at 2 to 4 week intervals after felling. The mean stain ...
Dian-Qing Yang, R Beauregard


The Effect on Biological and Moisture Resistance of Epichlorohydrin Chemically Modified Wood
2002 - IRG/WP 02-40224
Southern pine solid wood and fiber were chemically modified with epichlorohydrin to help in understanding the role of moisture in the mechanism of biological effectiveness of chemically modified wood. The solid wood had weight gains from 11% to 34%, while the fiber had weight gains from 9% to 75%. After modification, part of the specimens were water leached for 2 weeks or extracted for 2 hours ...
R E Ibach, B-G Lee


Performance of Tuff Brite C™ and other formulations against blue-stain, mold and brown-stain in freshly-sawn rubberwood (Hevea brasiliensis) in the humid tropics of Peninsular Malaysia
1997 - IRG/WP 97-30163
The relative anti-sapstain and anti-mold efficacies, including brown-stain development in freshly-sawn rubberwood (Hevea brasiliensis) in the Malaysian tropics, between selected water-based product concentrations of formulations Tuff Brite CTM without/with added Borax (at 1.5/0, 1/1.5, 1.5/1.5 and 2%/1.5%), PQ8TM without/with added Borax (at 2.5/0, 1.5/1.5, 2/1.5 and 2.5%/1.5%) and NeXgenTM/NeX-Br...
A H H Wong, T L Woods


Development of a disinfection treatment for oak logs to be imported from the USA
1984 - IRG/WP 3283
The European veneer industry depends greatly on oak supplies from the USA. To prevent the accidental introduction of the American Oak Wilt Disease (Ceratocystis fagacearum) into the member states of the EC, a disinfection treatment was developed for oak logs under consideration of the technical requirements of veneer production. Laboratory experiments and field trials in Germany and in the USA sho...
W Liese, M M Ruetze


A field test with anti-stain chemicals on sawn pine timber in Brazil
1989 - IRG/WP 3513
Field tests were carried out in São Paulo State - Brazil, to evaluate pre-treatment formulations of O-Phenyl-Phenol (OPP) based products. The Sodium Pentachlorophenate solution was used as a reference formulation. The treated test blocks were stacked in a covered shed, and in an uncovered place for air drying. The evaluation was done by measuring the percentage of stained surface on each test blo...
M B B Monteiro, S Brazolin


Properties of cellulose degraded by the brown rot fungus, Postia placenta
1988 - IRG/WP 1350
To gain further understanding of the nature of the cellulose depolymerizing agent or agents of brown-rot fungi, brown-rotted cellulose was chemically and physically characterized. Various culture conditions, such as low nitrogen and elevated oxygen levels, did not induce degradation of cellulose by Postia placenta in liquid cultures. Therefore, brown-rotted cellulose for analysis was prepared by a...
T L Highley, R E Ibach, T K Kirk


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


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