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In-ground durability of wood-based products – Comparative assessment of graveyard field tests and terrestrial microcosms
2014 - IRG/WP 14-20538
Traditionally wood durability and the effectiveness of wood preservatives are determined in so-called graveyard tests (according to EN 252 or AWPA E7). For laboratory testing, terrestrial microcosms (TMC) can serve as an alternative. Both tests have been applied for different types of wood-based materials, preservative treated, modified and unmodified wood. However, the usability of laboratory res...
A Soetbeer, L Meyer, C Brischke, P Larsson-Brelid, J Jermer


Effectiveness of Copper Indicators in Treated Wood Exposed to Copper Tolerant Fungi
2014 - IRG/WP 14-20554
Wood treated with a copper based wood preservative will typically turn a green color. While the depth of copper penetration can be readily discerned from the green color of the copper it is standard practice in research and commercial treating plants to make use of a color reagent such as Chrome Azurol S, Rubeanic acid or PAN indicator to reveal the penetration more clearly. When copper treat...
L Jin, K Brown, A Zahora, K Archer


Glutathione transferases and wood extracts
2015 - IRG/WP 15-10845
Wood rotting fungi have developed an efficient detoxification system allowing them to be well adapted to their way of life, these fungi generating and encountering potential toxic compounds during wood degradation. Belonging to this detoxification network, glutathione transferases are particularly extended in these fungi and their presence could reflect the chemical environments encountered by the...
A Deroy, F Saiag, Z Kebbi-Benkeder, N Touahri, M Morel-Rouhier, F Colin, S Dumarcay, P Gérardin,


Production of reactive oxygen species in the presence of oxidized iron and oxalate under conditions mimicking brown-rot fungal degradation of wood
2016 - IRG/WP 16-10861
The biochemical role of oxalate in the fungal solubilization of iron is well known, but additional information is needed on the role that oxalate plays in the brown-rot decay of lignocellulose. In this study iron sequestration from iron and iron oxide-hydroxides was assessed with regard to the function of a chelator-mediated Fenton (CMF) reaction and the generation of reactive oxygen species (ROS)...
Yuan Zhu, Liangpeng Zhuang, B Goodell, Jinzhen Cao, J Mahaney


Fungicidal Effect of Tobacco Stalks (Nicotiana tabacum L.) Against Brown Rot Fungi “Coniophora puteana”
2016 - IRG/WP 16-10865
In this study it was investigated that the fungicidal effect of tobacco plant (Nicotiana tabacum L.), which is grown in many countries of the world, against brown rot fungi “Coniophora puteana”. The protective effect of this plant on Scotch pine (Pinus sylvestris L.) wood was considered by means of fungi decay test (EN 113). The wood samples have been treated by five different extracts; at 3%,...
Ü C Yıldız, S Yıldız, A Yılmaz, S Durmaz


Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
Prevention of wood decay by fungi of the phylum Basidiomycota is an important issue of wood protection. The most destructive fungus in buildings in Central Europe is the dry rot fungus Serpula lacrymans, which needs more extensive restauration measures than all other wood decay fungi. Its close relative S. himantioides occurs more frequently in outdoor environment. The assessment and remediation o...
K Jacobs, N Rangno


Resistance of heat treated Ash wood under steam pressure: rot fungi, soil micro-organisms and termites
2016 - IRG/WP 16-40724
Thermal modification processes have been developed to increase the biological durability and dimensional stability of wood. The aim of this paper was to study the influence of ThermoWood® treatment intensity on wood decay resistance improvement against soil-inhabiting micro-organisms, brown/white rots and termite’s exposures. All of the tests were carried out in the laboratory with two differen...
K Candelier, S Hannouz, M-F Thévenon, D Guibal, P Gérardin, M Pétrissans, R Collet


The First Description of Soft-rot Cavity in Waterlogged Archaeological Woods by a Japanese Wood Anatomist Prof. F. Onaka in 1935
2017 - IRG/WP 17-10885
The term soft rot, which was caused by Ascomycetes and Deuteromycetes, was first used by J G Savory in 1954. Soft rot decay can be characterized by cavities within secondary cell walls align along the cellulose microfibrils. Our recent literature search revealed, however, that Prof. F. Onaka in Kyoto University described the soft rot cavities in the waterlogged archaeological woods in detail from ...
Yoon Soo Kim, K Yamamoto


Untreated and copper-treated wood soaked in sodium oxalate: Effects of decay by copper-tolerant and copper-sensitive fungi
2017 - IRG/WP 17-10888
Copper is widely used as the primary component in wood protectants because it demonstrates a broad range of biocidal properties. However, a key concern with using copper in wood preservative formulations is the possibility for brown-rot basidiomycetes to resist the toxic effect. Many brown-rot basidiomycetes have evolved mechanisms, like the production and accumulation of oxalate, which helps thes...
K M Ohno, G T Kirker, A B Bishell, C A Clausen


Agar block durability test of plywood made of neem (Azadiracta indica) leaves treated veneer using two white rot fungi
2017 - IRG/WP 17-40782
This study has been undertaken to investigate the durability of plywood made from non-durable wood (simul, Bombax ceiba) veneer treated by neem (Azadiracta indica, A. Juss.) leaves. The veneers were treated with cold and hot water solution of neem leaves of different concentrations (10%, 5%, 2.5%) and treatment durations (1, 2, 3 days) for cold water and 20, 40 and 60 minutes for hot water. The pl...
K Akhter, M A Hashem, S Akhter


Effects of exposure time on detection of the behavior of white-rot fungi on Oriental beech wood properties
2017 - IRG/WP 17-10902
The comparison of fungal degradation of Orientalis beechwood by two white-rot fungi was evaluated in this study. Biological, mechanical, and chemical assessments of beech wood were carried out at 30 day interval for 120 days. The results demonstrated that both fungi have greater effects on beech wood during each exposure time. However, Trametes versicolor degraded beech wood more effectively than ...
E Bari, D D Nicholas, K M Ohno


Mechanism of nano metal fluorides against fungi
2018 - IRG/WP 18-30730
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredient in wood preservatives such as copper and boron. Wood specimens treated with fluoride sols of MgF2 and CaF2 have been tested against brown-rot fungi (Coniophora puteana and Rhodonia placenta) in accordance with EN 113 and against termites (...
S M Usmani, K Klutzny, I Stephan, T Hübert, E Kemnitz


The biodegradation of creosote oil by various species of white rot fungi and bacteria isolated from the contaminated soil
2018 - IRG/WP 18-50334
The aim of the study was to determine biodegradation effectiveness of the selected polycyclic aromatic hydrocarbons (PAHs) contained in creosote oil, by two white rot fungal species (Bjerkandera adusta and Irpex lacteus) and three bacterial species (Pseudomonas putida, Pseudomonas sp. OS4 and Rahnella aquatilis). Bacteria were isolated from the soil contaminated with petroleum hydrocarbons from th...
M Sydow, J Zabielska-Matejuk, E Kaczorek, AStangierska, A Kropacz


From fungal detoxification systems to wood durability in neotropical forests
2019 - IRG/WP 19-10946
White-rot fungi are able to mineralize all the wood components due in particular to an efficient detoxification system. We hypothesised that components of this detoxification systems, glutathione transferases, could be used as tools to explore the natural durability of neotropical wood species. Analysis of the interactions between six glutathione transferases of Trametes versicolor and extracts of...
T Perrot, G Salzet, N Amusant, J Duchene, E Gelhaye


Characterization of Pectinases from Brown-rot Fungus Fomitopsis palustris
2019 - IRG/WP 19-10951
Brown-rot fungi occur on softwood used as building materials and cause destructive breakdown of wood structure. Therefore, a more accurate understanding is important from the perspective of wood protection. Previous studies have reported that hyphae of brown-rot fungi go through bordered pits on tracheids when the fungi grow into softwood [Francis W.M.R.Schwarze (2007)], and torus existing in the ...
Y Tanaka, N Konno, T Suzuki, N Habu


Impact of water holding capacity and moisture content of soil substrates on the moisture content of wood in terrestrial microcosms
2019 - IRG/WP 19-20662
Terrestrial microcosms (TMC) are frequently used for testing the durability of wood and wood-based materials as well as the protective effectiveness of wood preservatives. In contrary to experiments in soil ecology sciences, the experimental set-up is usually rather simple. However, for service life prediction of wood exposed in ground, it is of immanent interest to better understand the different...
C Brischke, F L Wegener


Biocidic effect of nano metal fluorides against brown-rot fungi
2019 - IRG/WP 19-30732
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredients in wood preservatives such as copper and boron. The effectiveness of nano metal fluorides was compared to sodium fluoride treated wood samples. Wood specimens treated with homo-dispersed sols of MgF2 and CaF2 were tested against brown-rot...
S M Usmani, K Klutzny, I Stephan, E Kemnitz


Relative efficacy of various oxine copper formulations against brown-rot fungi
2019 - IRG/WP 19-30741
This paper investigates the relative resistance of three different oxine copper formulations (oil-borne, water-borne, water-borne with pH-adjustment) as a preservative treatment for wood against brown-rot fungi. Impregnated southern pine sapwood cubes were exposed to R. placenta and G. trabeum cultures in a soil-block test. After eight weeks, the weight losses of the cubes were examined in relatio...
M Petruch, J Lloyd, A Taylor


FTIR analysis of wood blocks decayed by brown-rot fungi
2020 - IRG/WP 20-10959
Calibration curves of the relative lignin contents in Cryptomeria japonica decayed by brown-rot fungi were developed with Infrared Spectroscopy and Klason technique. First, wood decay test was conducted using brown-rot fungi (Fomitopsis palusris, F. pinicola, Wolfiporia cocos, Gloeophyllum trabeum, and Neolentinus suffrutescens) and white-rot fungus (Trametes versicolor) was used as a comparison. ...
R Kondo, Y Horikawa, R Kose, M Yoshida


The iron reduction by chemical components of wood blocks decayed by wood rotting fungi
2021 - IRG/WP 21-10979
Brown-rot fungi, a group of wood rotting fungi, is well known to be one of major microorganisms that cause the deterioration of wooden buildings in Japan and have been considered to use chelator-mediated Fenton (CMF) reaction in concert with hydrolytic and redox enzymes for degradation of wood cell wall. CMF can be described as a non-enzymatic degradation system that utilizes hydroxyl radicals pro...
R R Kondo, Y Horikawa, K Ando, B Goodell, M Yoshida


Mechanical and biological durability properties against soft-rot and subterranean termite in the field (grave-yard test) of beech wood impregnated with different derivatives of glycerol or polyglycerol and maleic anhydride followed by thermal modification in an opened or in a closed system
2021 - IRG/WP 21-40917
This paper presents mechanical and biological durability properties in soil beg test (soft-rot test) and field test (grave-yard test) against subterranean termite of the wood modified with an aqueous vinylic derivative of glycerol/polyglycerol or maleic anhydride cured in an opened or in a closed system. Wood modification was performed through impregnation of European beech (Fagus sylvatica) with ...
M Mubarok, H Militz, S Dumarcay, I W Darmawan, Y S Hadi, P Gerardin


Decay capacity and degradation patterns of Xylaria hypoxylon on different wood species
2022 - IRG/WP 22-10985
A host of physical and environmental factors may influence fungal decay including the wood substrate, temperature, moisture, oxygen, light, pH, and nitrogen. Understanding the effects of these factors on fungal decay is important for the effective utilization of wood decay fungi in biotechnological processes and for understanding the role of these organisms in global carbon cycling. The ascomycete...
E Bari, G Daniel, A Singh, J J Morrell


Changes in topochemistry and mechanical properties of Beech (Fagus orientalis L.) by natural fungus infestation
2022 - IRG/WP 22-10986
Beech is one of the major hardwood species in Europe. It is, however, highly susceptible to fungal attack both in the fresh state and during the storage. Understanding the alteration in chemical and mechanical properties of beech wood during the initial degradation state can lead to improved raw material utilization. Therefore, UV-microspectrophotometer (UMSP) and High-Performance Liquid Chromatog...
H Sivrikaya, M Rehbein, F Divos, S Adamopoulos, R Hosseinpourpia


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to dete...
E E Soytürk, S N Kartal


Studies into the effect of soil type and soil layer on the in-ground decay of European beech
2022 - IRG/WP 22-20681
In this study, European beech (Fagus sylvatica L.) specimens were exposed to three different soil types; Podsol (Podzol), Braunerde (Cambisol), and Pararendzina (Regosol), in adapted terrestrial microcosm (TMC) tests according to CEN/TS 15083-2 (2005). Soils were sampled (250 mm deep) from field sites and separated into their constituent layers to deliver three TMC setups; mineral soil layer only ...
B N Marais, S Kovacs, M Jansen, C Brischke


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