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The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10209
Field and fungal cellar trials have been set up to assess the biocontrol potential of a selected Trichoderma viride isolate in a situation representative of the end use of treated timber in ground contact situations. These trials are designed to give information about the efficacy of biological control as well as the suitability of existing chemical treatment methods for use with biocontrol fungi....
H L Brown, A Bruce


A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


The role of oxalic acid in short fiber formation by the brown-rot fungus Postia placenta
1993 - IRG/WP 93-10028
The mechanism by which brown-rot fungi depolymerize wood and cellulose remains a perplexing problem. Current evidence favors oxidation by low-molecular weight, non-enzymatic fungal metabolites. During attack of cotton cellulose by Postia placenta MAD-698, the degree of polymerization (DP) decreases to limit of degree of polymerization (LODP) over a period of 5-6 weeks with relatively low weight lo...
F Green III, J M Hackney, C A Clausen, M J Larsen, T L Highley


Micromorphological Characteristics of Degradation in Bamboo Attacked by White Rot Fungus Lentinus edodes
2006 - IRG/WP 06-10575
In comparison with micromorphological works for wood decay, little known is about the decay pattern of bamboos by wood decay fungi. The present work was undertaken to understand the general degradation pattern of bamboo and the influence of polylaminate layers in bamboo fiber walls on the restriction of fungal decay. The weight loss of bamboo species Phyllostachys puberscens against the white rot ...
Chang Hyun Cho, Kwang Ho Lee, Yoon Soo Kim


Micromorphology of Bamboo Fibers Degraded by Brown-Rot Fungus Gloeophyllum trabeum
2006 - IRG/WP 06-10576
The decay pattern of bamboo by brown-rot fungus was examined. In addition, the influence of polylaminate structures in bamboo fibers on the restriction of fungal decay was also investigated. The weight loss of bamboo Phyllostachys puberscens by the brown-rot fungus Gloeophyllum trabeum after 16 week incubation was about 25%. Parenchyma cells were severely degraded. Microscopical studies showed tha...
Kwang Ho Lee, Chang Hyun Cho, Yoon Soo Kim


Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki


Molecular characterization of M’jej, decaying agent of cedar forests in Morocco
2006 - IRG/WP 06-10593
Cedar wood is well appreciated since thousand years even though its economical importance is limited because of its world distribution restricted to some Mediterranean countries and Himalaya. The most important species, Cedrus atlantica have its biggest population in Morocco (130 000 ha) where it is submitted to fungal diseases. Among them, the locally named “M’jej” reduced notably saw mile ...
A Zaremski, S Bakkali-Yakhlef, C Chaintreuil, Y Abbas, Y Prin, M Abourouh, M Ducousso


Direct analysis from wood of the blue stain fungi Aureobasidium pullulans and Hormonema dematioides by denaturing gradient gel electrophoresis
2006 - IRG/WP 06-10595
Aureobasidium pullulans and Hormonema dematioides are the two organisms used in the EN 152 laboratory method for determining the effectiveness of preservatives against blue stain in service. The literature concerning the disfigurement of surface coatings and exposed timber in-service states that A. pullulans is the dominant blue stain fungus, due to its frequent isolation from stained material. In...
M J Ray, D J Dickinson


Resistance of pine and spruce heartwood against decay - The effect of wood chemical composition and coating with water-borne wood oil product
2006 - IRG/WP 06-10597
Natural durability of wood has been widely studied, but the combination of the natural durability and different treatments has not been the focus of many studies. The durability of wooden products is mainly based on the water permeability and the resistance against organisms. In this study, the water absorption and decay resistance of sapwood and heartwood of Scots pine and Norway spruce were exam...
H Viitanen, S Metsä-Kortelainen, T Laakso


Time-of-Flight Secondary Ion Mass Spectrometry Analysis of Polyethylene Glycol Penetration into Wood Cell Walls
2006 - IRG/WP 06-20328
Penetration of high molecular weight polyethylene glycol (PEG) into wood cell walls was investigated in red pine. PEG of different molecular weights (1000, 8000 and 20000) was labelled by bromination and treated samples were analyzed on Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) in dynamic mode. Both negative and positive spectra of the samples were collected in high spatial (bunche...
D Jeremic, P Cooper, P Brodersen


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl


High-energy multiple impact (HEMI)-test – Part 2: A mechanical test for the detection of fungal decay
2006 - IRG/WP 06-20339
The suitability of the high-energy multiple impact (HEMI)-test for detection of early fungal decay was examined. The HEMI – test characterizes the treatment quality of thermally modified wood by stressing the treated material by thousands of impacts of pounding steel balls. This method differentiates between heat treatment intensities, which are expressed by structural changes of the wood. Simil...
C R Welzbacher, C Brischke, A O Rapp


Water analysis in chemicals, wood or chemically treated wood using a new analytical method for selective water detection
2006 - IRG/WP 03-30391
Water or moisture content analysis can be a challenging task in many applications. However, knowing accurately the type of water which can be found in a given sample or product can provide important information about a chemical compound used for wood treatment and especially about the treated wood sample. Moisture content in wood plays an important role in fungal growth and it needs to be extensi...
R Craciun, D Taylor


Protection of hard and softwood through Neem leaves extracts and oil - A direction towards development of eco-friendly wood preservatives
2006 - IRG/WP 06-30394
Conventional wood preservatives like CCA and CCB are facing lot of criticism all over the world. It is essential to address the problem in view of environment protection. Eco-friendly wood preservatives may be considered as one option. The present study is an expolartion of neem leaves and seed oil against wood decaying fungi and termites. Azadirachta indica A. Juss, commonly known as neem is one ...
S Dhyani, S Tripathi


Thermal decomposition behavior of CCA-treated wood for safe disposal and the safe recovery of heavy metals through pyrolysis
2006 - IRG/WP 06-50238
If we could estimate the chemical changes in heavy metals by temperature in chromated copper arsenate (CCA)-treated wood during pyrolysis, it is expected that we could solve the environmental problems of heavy metals, which may occur during pyrolysis, and therefore, the thermal decomposition behavior of effective elements of CCA-treated wood was examined to find a safe disposal method. First, CCA-...
Dong Won Son, Dong-heub Lee, Sun-hae Cheon, Myung Jae Lee


Gypsum effects on ‘dry rot’ wood degradation as a function of environment
2007 - IRG/WP 07-10624
‘Dry rot’ fungi are a unique group of brown rot fungi that can degrade wood away from ground contact where other fungi fail to colonize. Successfully occupying this niche is partially due to efficient water and nutrient transport, but mobilizing elements, notably calcium (Ca) and iron (Fe), from adjacent building materials has also been implicated in their success. Here we report a series of t...
J Schilling, J Jellison


Bifenthrin recovery from glue-line-treated plywood
2007 - IRG/WP 07-20355
Plywood and laminated veneer lumber (LVL) can be protected from termite damage by addition of bifenthrin to the adhesive used in product manufacture. However, conditions associated with this manufacturing process may lead to deterioration or immobilisation of the active ingredient. In order to determine compliance with treatment specifications, it is important that the concentration of a preservat...
J Norton, L Stephens


Use of the Digital Refractometer for the On-site Analysis of Copper-based Preservative Systems
2007 - IRG/WP 07-20371
In some counties, there are no on-site chemical analysis methods available for treatment plants to use to determine the key active ingredients in the preservative treatment solutions. The complexity of the sophisticated methodology; cost of analytical instruments and training required for operating these instruments in treating plant level are the reasons for lacking such practice. In this paper...
L Jin, T Cashman, A Preston, J Trompetter, H Trompetter


Chelator-Mediated Fenton Chemistry in Wood Degraded by Fungi
2007 - IRG/WP 07-10618
Wood specimens were colonized by individual isolates of brown rot, white rot, soft rot and blue (sap) stain fungi. Ethyl acetate extracts of the ground wood were analyzed for their iron-reducing capabilities using a ferrozine-based assay. Extracts from wood colonized by brown rot fungi showed a significantly greater iron-reducing capability than extracts from wood colonized by white rot fungi or n...
B Goodell, G Daniel, J Jellison, Yuhui Qian


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP II: Results from field studies
2007 - IRG/WP 07-30429
The effects of Chlorothalonil (CTN) and Butylated Hydroxytoluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and the non-biocidal BHT was evaluated for its synergistic effects with CTN. ACQ-C was a positive control and untreated SYP sta...
G T Kirker, M L Prewitt, S V Diehl


Multivariate analysis of infrared spectra for the prediction of the mechanical performance and dimensional stability of thermally modified wood
2007 - IRG/WP 07-40367
The use of calibrated mid-infrared Fourier transform (FTIR) spectroscopy for predicting the main bending strength parameters of thermally modified wood is described for the first time. The analysis is based on the handling of OSC-filtered FTIR data from control and treated wood samples, using partial least squares (PLS) regression. Sets of Norway spruce, Scots pine and Beech wood samples were th...
M M González-Peña, M D C Hale


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


Genome-wide survey of cellulase related genes of white rot fungus,Pleurotus ostreatus
2007 - IRG/WP 07-10627
A white rot fungus, Pleurotus ostreatus, which is a important edible mushroom, has received much attention to apply to bioremediation and bioconversion because it has both cellulase and ligninase. To confirm copy number of cellulase-related genes encoded in P. ostreatus genome, we attempted to genomic Southern hybridization of P. ostreatus. The draft genome sequence and a large quantity of EST and...
T Tamenori, S Horisawa


White Rot in Eucalyptus Wood: Anatomical Changes and Density Variation by X-Ray Densitometry
2007 - IRG/WP 07-10631
Wood samples of the hybrid Eucalyptus grandis x urophylla attacked by white rot fungi were characterized. Microscopically, the colonization of the fungi was observed in the vessels and radial parenchyma, however the cell walls of the some fibers were attacked, indicating susceptibility to this fungi. Zone lines were observed as a result of interaction and compartmentalization of the fungi. Microsc...
M Tomazello Fo, S Brazolin, M P Chagas


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