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The true dry rot fungus (Serpula lacrymans) as a wound parasite of living Norway spruce (Picea abies) in the Czech Republic
2001 - IRG/WP 01-10389
Some late 19th century Central European mycologist reported finds of the true dry rot fungus (Serpula lacrymans) from nature. They even suggested that it could be a parasite of living trees. However examination of herbarium material has shown that the species they found was thin-fleshed dry rot (Serpula himantioides) rather than Serpula lacrymans. In 1992 the Czech mycologist Kotlaba reported find...
J Bech-Andersen, S A Elborne, J Andreasson, J Ch Sterler


A comparative analysis of Coniophora olivacea (Fr. ex Pers.) Karst. and Coniophora puteana (Schum. ex Fr.) Karst. test strains
1993 - IRG/WP 93-20004
Investigations were carried out to compare pure cultures of Coniophora olivacea (Fr. ex Pers.) Karst. used as a test fungus in Australia and other Pacific countries, and Coniophora puteana (Shum. ex Fr.) Karst. which is used in Europe. Comparisons included morphology, growth rate and dry mass of mycelium, decay capacity, influence of temperature, toxic value of CCA and quaternary ammonium compound...
J Wazny, L J Cookson


A review of the implementation of results-based standards for preservative treated timber
1999 - IRG/WP 99-20156
European standards for the specification of preservative treated timber are written in terms of the results achieved in the commodity by the treatment process. This is described in terms of penetration and retention of preservative. Results from a study on the commercial application of this approach in the UK show the difficulties associated with applying the new European standards; particularly w...
E D Suttie, A S Hughes, R J Orsler


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Changes in some important mechanical and physical properties of untreated and treated mining timber after exposure to the conditions of a colliery
1996 - IRG/WP 96-20082
Each of salt treated and untreated mining timber (wedge) pairs with 5x15 cm² cross-section and 155 cm length prepared from Scots pine, Bornmüller's fir and oriental beech was cut in the small standard test specimens to determine changes in some important mechanical and physical properties, 13 month after exposure to the conditions of Kozlu Hard Coal Mines in the depth of 52 m. Thus, the...
A Bobat


Chemical analysis of wood waste - The problem of sampling
1999 - IRG/WP 99-20168
A quantitative analysis of the content of active substances in wood is often demanded in quality control after processing and for the proper waste management. As a measure for quality control, analysis has the advantage that the type of preservative is known and assumptions can be made about its distribution in the treated wood.W aste wood may have been treated by a wide range of organic and inorg...
A Peylo, R-D Peek


Serpula lacrymans the dry rot fungus. Revue on previous papers
1989 - IRG/WP 1393
It is found that the Dry rot fungus Serpula lacrymans grows in houses only because of its need for basic materials to neutralize the oxalic acid production or heavy metals which celate the oxalic acid. The average distance from the mycelium to the basic materials is found in average to be 14.2 cm with a variation from 0-100 cm. In contrast to Serpula lacrymans the Coniophora puteana and the Rigido...
J Bech-Andersen


Production of monoclonal antibodies to Serpula lacrymans and their application in immunodetection systems
1993 - IRG/WP 93-10004
Monoclonal antibodies were produced against both whole cell mycelial extracts and exo-antigen extracts of the causative organism of dry rot, Serpula lacrymans. The antibodies were tested for cross-reactivity against fifteen strains of Serpula lacrymans and nine other fungal species. These species represented either other wood decay fungi or non-decay fungi commonly co-isolated with Serpula lacryma...
H Glancy, J W Palfreyman


Screening of fungal strains for wood extractive degradation
1998 - IRG/WP 98-10254
Fungal strains were screened for their ability to degrade apolar extractives in wood from scots pine. The degradation of total wood extractives by 91 different strains was monitored in stationary batch assays incubated for 6 weeks. The results obtained show that the ability of wood-inhabiting fungi to utilize wood extractives varied greatly, even for different isolates of the same species. Fungal ...
J Dorado, M J Martinez-Inigo, T A van Beek, F W Claassen, J B P A Wijnberg, R Sierra-Alvarez


Preliminary investigation on the natural durability of Guayule (Parthenium argentatum)-based wood products
2000 - IRG/WP 00-40154
Conventional preservatives used to protect wood from insect and microbial damages are presently of major concern to human health and the environment. Finding alternative and economical preservatives has not been successful. Previous studies have shown that the resinous material extracted from the guayule plant (Parthenium argentatum, Gray) has both insect- and microbial-resistant properties. Unfor...
F S Nakayama, P Chow, D S Bajwa, J A Youngquist, J H Muehl, A M Krzysik


Effect of remediation on the release of copper, chromium, and arsenic from particleboard made from CCA treated wood
2001 - IRG/WP 01-50170
This study sought to determine the effect of remediation with oxalic acid (OA) extraction and Bacillus licheniformis fermentation on the release of copper, chromium, and arsenic from particleboard made from remediated wood particles and also investigates durability of the particleboard against white and brown- rot fungi. Particleboard samples were manufactured using untreated, CCA-treated, OA-extr...
S N Kartal, C A Clausen


An analysis of the effects of some factors on the natural durability of pine (Pinus sylvestris L.) and spruce (Picea abies Karst.)
1986 - IRG/WP 1279
The effects of some factors on the natural resistance of pine and spruce sapwood against fungal decay and against attack of house-longhorn beetle larvae have been studied in laboratory tests and the results are evaluated by analysis of variance and regression analysis. Following conclusions were reached: Wood from summer-felled trees did not have a lower inherent natural durability against fungal ...
J B Boutelje, T Nilsson, S Rasmussen


Free radical process controlled by manganese peroxidase and lipid-related metabolites produced by Ceriporiopsis subvermispora
2001 - IRG/WP 01-10412
Ceriporiopsis subvermispora, a specific lignin-degrading fungus produced free unsaturated fatty acids (USFAs) including 9,12-octadecaienoic asid, together with saturated fatty acids (SFAs) at an incipient stage of cultivation on wood meal cultures. In prolonged cultivation period after two weeks, the amount of intact fatty acids decreased with increasing in organic hydroperoxide and TBARS producti...
T Watanabe, M Enoki, S Sato, Y Honda, M Kuwahara, N Shirai, K Messner


Le pin: Matériau à contact alimentaire [Pine wood as a material for food contact]
1995 - IRG/WP 95-50040-01
Monoterpenes emitted to air from woods of pine (Pinus nigra, Pinus laricio, Pinus pinaster and Pinus sylvestris) were determined on the one hand by static analysis and by other hand by sampling on the Tenax adsorbent, followed by analysis using thermal desorption, both being combined with gas chromatography coupled with mass spectrometric detection. The major monoterpenes were alpha-pinene and 3-c...
A Waymel, M Lamour, G Labat


Neem leaves, a potential source for protection of hardwood against wood decaying fungus
2005 - IRG/WP 05-30370
Neem leaves are widely recognized for their insecticidal, pesticidal, fungistatic and fungitoxic ability. The present study is to investigate the efficacy of neem leaves extractives against wood destroying microbes, a brown and a white rot. Neem leaves extractives prepared in various solvents such as petroleum ether, acetone, ethanol, methanol and hot water were tested at a concentration levels of...
S Dhyani, S Tripathi, V K Jain


Degradation of resin constituents in various wood species by the white rot fungus Bjerkandera sp. strain BOS55
1999 - IRG/WP 99-10301
In previous studies, the white-rot fungus Bjerkandera sp. strain BOS55 was shown to cause extensive degradation of lipophilic extractives (resin) in Scots pine wood. Further research was carried out in order to investigate the ability of Bjerkandera sp. for reducing resinous constituents in various softwood (Douglas fir, larch and spruce) and hardwood species (birch, beech and poplar). The greates...
J Dorado, T A Van Beek, F W Claassen, R Sierra-Alvarez


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


A model for attack at a distance from the hyphae based on studies with the brown rot Coniophora puteana
1995 - IRG/WP 95-10104
In timber infested by brown rot fungi, a rapid loss in strength is attributed to production of hydroxyl radicals (HO·) at a distance from the hyphae. The immediate precursor is Fenton's reagent (Fe(II)/H2O2), but the pathways leading to Fe(II) and H2O2 have remained unclear. Cellobiose dehydrogenase, purified from cultures of Coniophora puteana, will couple oxidation of cellodextrins to ...
S M Hyde, P M Wood


Isolation of a gene from the melanin pathway of the sapstaining fungi Ophiostoma piceae using PCR
1997 - IRG/WP 97-10219
To prevent sapstaining fungi from discoloring wood, it is necessary to determine what factors affect the biosynthesis and characteristics of the pigment(s) and to identify the genes involved in the pathway. Using inhibitors and heterologous DNA probes from Alternaria alternata, we suggest that melanin, the pigment of Ophiostoma piceae, is produced by the dihydroxynaphthalene (DHN) pathway. Recentl...
R Eagen, J Kronstad, C Breuil


An investigation of the nutritional physiology of the wood-boring weevil Euophryum confine Broun
1994 - IRG/WP 94-10082
The digestion of Picea abies Karst and Pinus sylvestris L. by Euophryum confine, at two sites is reported in this study. The composition of sound wood, wood from the heads of the weevil tunnels and the frass egested has been determined by gravimetric analysis. A comparison of sound wood and wood ingested showed that weevils tunnelled in microbially decayed wood, while a comparison of the wood inge...
A J Pitman, S M Cragg, G S Sawyer


The micro-distribution of copper/chrome/arsenate in Acer pseudoplatanus and Eucalyptus maculata
1973 - IRG/WP 319
The excellent field performance of copper-chrome-arsenate (CCA) treated timber has been accepted for many years. The preservative loadings used in practice have been based on field trial results, backed by service tests. The performance of treated hardwoods in trials and practice indicated that provided the required loading and penetration could be achieved the performance would be good. Recent un...
D J Dickinson


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


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