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rDNA-ITS sequence of Serpula lacrymans and other important indoor rot fungi and taxon-specific priming PCR for their detection
1999 - IRG/WP 99-10298
Taxon-specific priming polymerase chain reaction (TSPP) is a powerful molecular tool for fungal diagnosis. For its application to indoor rot fungi, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) of the main fungal species causing wood rot in European buildings was amplified with the polymerase chain reaction (PCR). The ITS region was sequenced. The complete sequences are present...
O Schmidt, U Moreth


Ultrastructure of Prumnopitys ferruginea wood from a buried forest in New Zealand
1991 - IRG/WP 1489
The buried wood of Prumnopitys ferruginea, a New Zealand native tree, was examined by scanning and transmission electron microscopy to investigate the extent to which the wood had deteriorated and the cause of its deterioration. Examinations showed that some deterioration of wood had occurred. Microscopy revealed a decay pattern similar to that produced by cavitation bacteria. Lenticular cavities ...
A P Singh, T Nilsson, G F Daniel


16S rRNA Analysis of the Bacteria Associated with Biocide Degradation
2004 - IRG/WP 04-10543
The bacterially mediated degradation of the new anti-fungal biocide, bethoxazin, was studied in vitro, by means of 16S rRNA PCR-amplification and cloning techniques. Woodblocks impregnated with a subtoxic concentration of bethoxazin were incubated in compost, and the micro-organisms associated with the wood after 6 and 12 weeks was studied. It was found that after 6 weeks the wood contained a la...
D F Wallace, D J Dickinson


Colonization of treated and untreated ponderosa pine exposed in Hilo, Hawaii
1995 - IRG/WP 95-20068
The rate of decay in above ground exposures is largely controlled by rainfall and temperature, factors which can be used to construct a climate index of decay hazard in above ground exposures. Developers of new biocide formulations have utilized this knowledge by establishing test sites in sub-tropical regions such as the Gulf Coast of the United States. More recently, field sites have been locate...
C M Freitag, J J Morrell, K J Archer


Arbeitsverfahren zum Herstellen und mikroskopischen Auswerten von Dünnschnitten aus Holz-Bohrkernen. [Working method for producing and evaluating microscopically thin cuts of bore samples]
1980 - IRG/WP 2133
Bore samples are important specimens; e.g. they are in use to investigate poles in service. On the basis of known methods of microtomy a technique is described which permits the production of longitudinal cuts from bore samples. For this purpose, the bore sample is glued onto a mounting; the surface of cut is coated with a polystyrene solution. The cut thus consolidated can be stained, drained, an...
S Cymorek


Investigations into the biology of Meruliporia incrassata
2004 - IRG/WP 04-10508
The dry rot fungus Meruliporia incrassata (Berk. and Curt.) Murr. is a highly destructive brown rot wood decay fungus and is a significant pest of wooden structures. The fungus, know commonly as ‘Poria’, is characterized in culture by strand mycelium and skin-like surface mycelium. In structural environments it is found to produce prominent water conducting rhizomorphs, is a copious spore prod...
J Jellison, C Howell, B Goodell, S L Quarles


Silicic acid-Boric acid complexes as wood preservatives
2001 - IRG/WP 01-30273
A silicic acid monomer aqueous solution (SAMS) or colloidal silicic acid solution (CSAS) was combined with various metal compounds or boric acid. Agents where SAMS or CSAS was combined with boric acid gave good protection against decay caused by the brown-rot fungus Fomitopsis palustris, the treated wood (Cryptomera japonica D. Don) specimens after the leaching test maintained a high resistance to...
H Yamaguchi


An alternative method for the determination of lignocellulolytic enzymatic activities of white rot fungi
1998 - IRG/WP 98-10261
Some lignocellulolytic activities of wood decay fungi can be evaluated in an indirect way through the study of the decolorization that white rot fungi produces in culture media in the presence of dyes. In this assay, ten dyes were selected, which were added in concentrations of 0.00 1% - 0.003% (P/V) to four-day cultures of Trametes versicolor containing 1% of beech sawdust in a basal salt medium...
D Muñoz-Mingarro, F Llinares, F Rubio, M Yuste, C Rodríguez-Borrajo, J Jiménez, M T De Troya


Bending properties of wood after its decay with Coniophora puteana and subsequent modification with selected chemicals
1999 - IRG/WP 99-40146
Mechanical properties of wood are often decreased due to decay processes caused by biotic and/or abiotic factors. Damaged wooden elements (e.g. historical structures) can be reinforced by more methods, including their modification with convenient chemicals. This paper presents influences of selected chemicals on basic bending properties (modulus of elasticity - MOE, modulus of rupture - MOR) of so...
L Reinprecht, S Varinska


Evaluation of bending strength by non-destructive methods of Ezomatzu with white pocket rot
1991 - IRG/WP 2371
The possibility of application of three non-destructive methods was examined for evaluation of bending strength of ezomatsu (Picea jezoensis CARR.) with white pocket rot. As non-destructive methods, the measurements of ultrasonic wave propagation time, impact-induced stress wave propagation time and penetration depth of pin of "PILODYN Wood Tester" were applied to compare with visual evaluation of...
T Tanaka


Wood structure in relation to excessive absorption - a literature survey
1971 - IRG/WP 300
This literature study, composed in connection with 'the structure of wood in relation to the excessive absorption', started from the idea that the subject only could be dealt with successfully if the structure was taken into account in relation to a normal absorption pattern in so far as this knowledge can enlighten the understanding of the abnormal situation. The normal penetrat...
S M Jutte


The production of extracellular hydrogen peroxide by some brown-rot fungi
1990 - IRG/WP 1446
The role of hydrogen peroxide (H2O2) has been discussed in the degradation of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by detecting the oxidation of the chromogen 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horse radish peroxidase (HPR). ABTS and HPR were added to a solid wood based culture media. In this study ...
A-C Ritschkoff, L Paajanen, L Viikari


Assessment of the effects of rentex remedial treatment on some wood pole inhabitant micro-organisms
1994 - IRG/WP 94-30053
The effects of a remedial ground-line treatment using Rentex, a stabilised paste containing a mixture of fluoride and dichromate salts, on the micro-organisms inhabiting a sample of some 160 creosoted, on-line, electricity distribution poles, have been investigated as part of an appraisal of the efficacy of this remedial treatment. Half of the poles were treated and the other half used as controls...
D C R Sinclair, G M Smith, A Bruce, H J Staines


Fungal detoxification of organotin biocides
1985 - IRG/WP 1258
The ability of a range of wood decaying fungi to inactivate bis(tri-n-butyltin) oxide (TnBTO) in the extracellular growth medium, in stationary liquid culture was determined. A distinction between the ability to tolerate the fungicide and to inactivate it was made: the white-rot organism Coriolus versicolor being the most efficient inactivator. In an attempt to determine the extracellular agents r...
P S Belford, D J Dickinson


A method test of chemicals for the treatment of walls infected with Serpula lacrymans
1992 - IRG/WP 92-2408
A method, which has been used in the UK for over 25 years, is described for assessing the effectiveness of products for application to walls during remedial treatment to eradicate the dry rot fungus Serpula lacrymans. Approvals for products previously used for this purpose in the UK are being changed. Approval for tri n-butyltin oxide has been withdrawn, and PCP derivatives can continue to be used...
J K Carey


Cation analysis of wood degraded by white and brown rot fungi
1992 - IRG/WP 92-1552
Changes in the concentrations and composition of cations within wood colonized by the brown-rot fungi Postia placenta and Gloeophyllum trabeum and the white-rot fungi Trametes versicolor and Phanaerochaete chrysosporium were analyzed using plasma emission and atomic absorption spectrophotometry. In degraded poplar wood significant decreases in pH and electrical resistance readings were observed as...
J Jellison, K C Smith, W T Shortle


Resistance of acrylic paints on wood against growth of the rot fungus Dacrymyces stillatus
1990 - IRG/WP 2345
In the last few years the presence of the rot fungus Dacrymyces stillatus has been repeatedly confirmed in external wood panels, particularly from wood painted with water based paints. A laboratory method for testing of the fungal resistance of paint films on wood has been developed.This method has been used to test the efficacy of the fungicides Parmetol DF 18 and Parmetol HF 25 against attack by...
J Bjurman


Extracellular osmiophilic particles in connection with brown rot and white rot
1982 - IRG/WP 1157
The way of attack on Pine woodblocks of two brown-rot fungi (Fomitopsis pinicola, Coniophora puteana) differing in their cellulase activities, and one white-rot fungus (Trametes hirsuta) has been investigated by transmission electron microscopy. Even at early stages of decay, osmiophilic particles were found with all the fungi investigated. In the brown-rotted wood, the osmiophilic particles occur...
K Messner, H Stachelberger


Minimum inhibitory concentration of methylene bisthiocyanate towards selected wood inhabiting fungi in liquid culture and on radiata pine
2001 - IRG/WP 01-30261
The objective of the current study was to determine the minimum inhibitory concentration (MIC) of methylene bisthiocyanate (MBT) towards wood inhabiting fungi common on unseasoned radiata pine (Pinus radiata D. Don). The fungi used were Ophiostoma floccosum, Leptographium procerum, Sphaeropsis sapinea and Trichoderma viride. Fungi were individually inoculated into nutrient media spiked with differ...
T Singh, B Kreber, C Chittenden, R N Wakeling, A Stewart


Production, function and neutralization of oxalic acid produced by the dry rot fungus and other brown rot fungi
1987 - IRG/WP 1330
The formation of oxalic acid by the wood-destroying fungi causing brown rot, is found to be the key which by hydrolysing the hemicellulose brings the cellulose in the tracheid wall in contact with the cellulase enzymes and yeld watersoluble sugars leaving only a lignin skeleton. To control the pH in the substrate the excess oxalic acid is precipitated to water insoluble calcium oxalate by the dry ...
J Bech-Andersen


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


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