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Confocal Laser Scanning Microscopy (CLSM) of decayed wood
1998 - IRG/WP 98-10273
Confocal laser scanning microscopy (CLSM) is currently being used to examine biodegraded wood and is proving to be a useful new technique in this area of work. Non-invasive optical sectioning within a thick specimen (20 - 50 µm), coupled with post image processing techniques allows manipulation of images and 3-D reconstruction from serial sections. Glutaraldehyde can cause cell autofluorescence,...
Ying Xiao, R N Wakeling, A P Singh


Control of sapwood-inhabiting fungi by fractionated extracellular metabolites from Coniophora puteana
1991 - IRG/WP 1494
The objective of this study was to test the fractionated metabolites released by Coniophora puteana for their antagonistic activity against the sapstain fungi Ceratocystis coerulescens and Aureobasidum pullulans, and the molds Asperigillus niger and Penicillium spp. The acetone-soluble fraction obtained from the culture filtrate prepared from Coniophora puteana grown on 6% malt extract agar inhibi...
S C Croan, T L Highley


A novel solvent penetration assessment technique for wood preservativation treatments using waterborne systems
1990 - IRG/WP 2346
Solvent and hence solute (a.i.) penetration during any wood preservation treatment cycle and the flow pathways taken by the solvent in the wood are crucial elements in determining the adequacy of any treatment. Inadequate solvent penetration into specimens or an inappropriate tissue throughflow pattern during impregnation will markedly affect the distribution pattern achieved by many non-diffusibl...
A J Pendlebury, J Coetzee, E Sorfa, A Botha


Microbial fouling of 10-40% naphthalene in creosote treated and untreated wooden pilings in the marine environment - A progress report
1980 - IRG/WP 455
In June 1978, Document No: IRG/WP/442 entitled 'Microbial Biofouling of 10-40% Naphthalene in Creosote Treated and Untreated Wooden Pilings in the Marine Environment" was released to the IRG members as a report on the early results of a long term (about 5 years) study of whether the naphthalene content of creosote was importart in protecting wood pilings in the marine environment from bor...
R R Colwell, A J Emery


Decay resistance of densified ammonia-plasticized stems of oil palm (Elaeis guineensis)
1991 - IRG/WP 3673
When wafers of oil palm stems (Elaeis guineensis) were plasticized with 28% aqueous ammonia and immediately compressed mechanically, the treated material, gained 73% in basic density (average basic density, 0.695 g/cm³), and was highly resistant to decay by wood rot basidiomycetes. Compared with the control specimens (density, 0.403 g/cm³), resistance to decay of the densified specimens by Corio...
A H H Wong, M P Koh


The dry rot fungus (Serpula lacrymans) in nature and its history of introduction into buildings
1999 - IRG/WP 99-10300
For many years the True dry rot fungus (Serpula lacrymans (Wulf.: Fr.)Schroet.) has exclusively been found in buildings. That is why it is called the True dry rot fungus. The origin of the fungus has always been a mystery, but a wild ancestor must have occured. In the literature there is some information about finds of Serpula lacrymans in nature, however it is difficult to distinguish it from the...
J Bech-Andersen, S A Elborne


Dimensional lumber model demonstrates the sensitivity of the particle capture immunoassay in early detection of brown-rot fungi
1995 - IRG/WP 95-20058
A dimensional lumber model was designed to test the sensitivity of the particle capture immunoassay (PCI) compared to traditional methods of culturing and microscopic examination for detection of brown-rot fungi. Southern yellow pine 2 x 4's (5.1x10.2 cm²), and 86.4 cm in length, were inoculated on one end with the brown-rot fungus, Postia placenta, and tested over the full length of the...
C A Clausen, L Ferge


Detection of brown-rot antigens in southern pine
1996 - IRG/WP 96-20090
Brown rot fungal antigens were detected by particle capture immunoassay (PCI) in southern pine 2 x 4's beyond visible or culturable hyphal growth. Further analysis of test samples revealed changes along the 2 x 4's that could be grouped into zones. Zone 1, the point of inoculation through 6 cm, showed low pH, measurable oxalic acid, high moisture, and high protein. Zone 2, throug...
C A Clausen


Lignin degradation by a non-enzymatic system supposed to be active in white rot fungi
2000 - IRG/WP 00-10340
Electron microscopic investigations have shown that ligninolytic enzymes of white-rot fungi are only able to penetrate the wood cell wall in late stages of degradation. Thus, the selective degradation of lignin of certain white-rot fungi can only be explained on the basis of a low molecular weight, highly diffusible system. A system, consisting of copper, a coordination compound and either H2O2 or...
P Lamaipis, W Gindl, T Watanabe, K Messner


Hemicellulosic induction of oxalic acid in Postia placenta
1994 - IRG/WP 94-10060
Most studies suggest that enzymes produced by brown-rot fungi are too large to penetrate sound wood structures, even after decay begins. Thus, nonenzymatic agents have been proposed to initiate brown-rot decay. We have reported that the brown-rot fungus Postia placenta MAD-698 initiates a 2-fold decrease in wood pH within 7 days of colonization which is mediated by production of oxalic acid. Strai...
F Green III, M Larsen, T L Highley


Aspects of leaching
1976 - IRG/WP 267
With respect to Document No: IRG/WP/246, and the recommendation that an economic leaching test can be based on a non-changing volume of water (500 ml/block), I would like to relate our experiences with this suggestion. Following 14 days of continuous immersion in de-ionized water it was found that blocks treated with moderate and above concentrations of a preservative gave no problems, but those b...
R S Smith


Advances in understanding the mode of action of methylene bisthiocyanate against Sphaeropsis sapinea
2002 - IRG/WP 02-30284
The importance of arresting sapstain pre-infection in radiata pine in New Zealand, to prevent downstream sapstain in both logs and sawn timbers has been clearly demonstrated. Efforts are being made to improve the efficacy of diffusible antisapstain agents through improved understanding wood/fungicide/fungus interactions. The mechanism of action of methylene bisthiocyanate (MBT) was investigated u...
T Singh, R N Wakeling, B Kreber, A Stewart


A bibliography of the dry rot fungus, Serpula lacrymans
1988 - IRG/WP 1337
For many decades the occurrence of dry rot in buildings has been the cause of serious concern in temperate regions of the world. Consequently, much effort has concentrated on determining the morphology of dry rot and on finding means of prevention and control. During early studies, observers tried to understand the phenomenon of dry rot as a whole, and in the course of the 19th century aspects cha...
G Seehann, B M Hegarty


Decay resistance of Platanus acerifolia wood against White Rot Fungi (Coriolus versicolor)
2002 - IRG/WP 02-10431
Degradation of the lignocellulose complex in wood varies on the microorganism causing decay and chemical composition of wood. The weight loss of Platanus acerifolia wood after decay by white rot fungus (Coriolus versicolor) for period 16 weeks were evaluated according to European standard EN 113 and beech wood as a control. The samples were collected in October 2000 from Nantes, France. The aim of...
M Irmouli, O Duchelier, N Ayadi, B Charrier


The long road to understanding brown-rot decay. A view from the ditch
1995 - IRG/WP 95-10101
Interest in understanding how brown-rot fungi decay wood has received increasing interest in recent years because of a need to identify novel targets that can be inhibited for the next generation of antifungal wood preservatives. Brown-rot fungi are unique in that they can degrade holocellulose (cellulose and hemicellulose) in wood without first removing the lignin. Furthermore, they degrade holoc...
F Green III, T L Highley


Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Micromorphology of Schizophyllum commune attack in pine (Pinus sylvestris) wood
1983 - IRG/WP 1184
The decay pattern caused by Schizophyllum commune in pine latewood tracheids was studied using both light and transmission electron microscopy. The attack began as isolated concentric slits within the S2 layer with extensive lamellation and separation of individual wall layers observed in subsequent stages of decay. The slits resulting from attack appeared to be formed in thin, regular concentric ...
T Nilsson, G F Daniel


Respiration measurement of dry-rot
1995 - IRG/WP 95-10095
Methods for measuring the production of carbondioxide from wood infected with the dry-rot fungus were evaluated. By enclosing whole wood blocks and measuring the concentration of gas in the headspace by gas chromatography, an exponential increase in the concentration of CO2 was observed for at least 2 months. This technique could therefore present a method for evaluating various treatments of dry-...
L Toft


Borate-treated food affects survival, vitamin B12 content, and digestive processes of subterranean termites
1990 - IRG/WP 1448
Toxicity of boron compounds was studied by analyzing survival rates and vitamin B12 contents in Formosan subterranean termites, Coptotermes formosanus Shiraki, that were exposed to dietary treatments for 10 days. The dietary treatments applied in moistened cellulose were (a) 0.05% boric acid equivalent (BAE) of disodium octaborate tetrahydrate, (b) 0.05% BAE of anmonium pentaborate plus sodium sul...
L H Williams, S I Sallay, J A Breznak


On the biotope of dry rot (Serpula lacrymans) in the wild
1995 - IRG/WP 95-10123
The True Dry rot fungus was found in the Himalayas several times in this century. The finds were able to cross breed with Serpula lacrymans from Denmark and therefore all belong to this species (Harmsen 1960). In order to understand its successful colonisation in buildings we were interested in studying the natural biotope, i.e. the moisture, temperature and soil relationships. All together 15 fru...
J Bech-Andersen, S A Elborne, K Bech-Andersen


The effect of water storage on the cell-structure of Sitka spruce (Picea sitchensis) with reference to its permeability and preservation
1970 - IRG/WP III 3A
Commercial treatment trials with full sized transmission poles have shown that water storage considerably improves the permeability of Sitka and Norway spruce. These results were confirmed by laboratory measurements which showed that in the sapwood of ponded Sitka spruce there is a vast improvement in permeability in all three grain directions. Heartwood permeability does not seem to be affected t...
J A Dunleavy, A J McQuire


Preliminary PIXE micro-analysis of copper-chrome-arsenic preservative treated Malaysian hardwoods
1999 - IRG/WP 99-20169
The novel nuclear microscopy, essentially the Particle-induced X-ray Emission (PIXE) system, was applied for examining the macro-distribution and micro-analysis of copper-chrome-arsenic (CCA) preservative in Malaysian hardwoods Koompassia malaccensis, K. excelsa, Alstonia spp. and Dipterocarpus spp., and one temperate pinewood Pinus sylvestris, where the preservative retention differed between the...
A H H Wong, R B Pearce, G W Grime, F Watt


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


Sapstain fungi associated with soft wood species in Kerala, India
1998 - IRG/WP 98-10260
Most of the packing case, plywood and match industries distributed throughout Kerala State, India utilise different timber species. The major problem in the utilisation of these species is their susceptibility to fungal sapstain and mould growth due to the conducive climatic conditions in the State. Several wood-based industries were surveyed and based on the economic importance and extent of saps...
E J M Florence, J K Sharma, R Gnanaharan


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


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