IRG Documents Database and Compendium


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EELS (Electron Energy Loss Spectroscopy) - a technique for quantification of nitrogen and other light elements in the cell wall
1999 - IRG/WP 99-20163
A literature survey was performed to find progress in techniques for monitoring penetration of synthetic resins in wood cell walls. Electron energy loss spectroscopy (EELS) in combination with transmission electron microscopy (TEM) was successfully applied for the high resolution examination of the distribution of a partly methylated hydroxymethyl melamine resin in Norway spruce (Picea abies Karst...
A O Rapp, H Bestgen, W Adam, R-D Peek


Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch


Biosynthesis of ß-Glucan microfibrils by cellular fractions from brown-rot fungus Postia placenta (MAD-698 and ME-20) and white-rot fungus Schizophyllum commune (MAD-619)
1993 - IRG/WP 93-10025
In this study, we compared the brown rot fungus Postia placenta (MAD-698 and ME-20) with the white rot fungus Schizophyllum commune (MAD-619) to determine the location and distribution of glucan synthetase. We also measured the soluble protein content in subcellular fractions obtained by differential centrifugation MAD-698 is a degradative isolate, but ME-20 and MAD-619 do not produce significant ...
S C Croan, T L Highley


Durability of plywood made from soft- and hardwoods assessed according to ENV 12038 after artificial and natural ageing
2000 - IRG/WP 00-20191
Plywood was prepared from Norway Spruce and pure heartwood of Douglas Fir, Scots Pine, European Oak, False Acacia and Macoré using a phenol-formaldehyde glue. The panels of 38 mm in thickness were pre-conditioned according to the following procedures: a) 12 weeks storage at 20°C/65% rh, b) 2 weeks artificial weathering (with rain and UV-radiation at changing temperatures), and c) a natural weath...
H Leithoff, R-D Peek


A study of decay type variability in variously treated Fagus sylvatica and Pinus radiata field test stakes exposed at a vineyard for 30 - 45 months
1998 - IRG/WP 98-10271
Pinus radiata test stakes were treated with 10 kg/m3 of CCA plus 4 lower retentions in a geometric series of 1.5. Fagus sylvatica was treated with 15 kg/m3 and 2 lower retentions. Both timber species were also treated with equivalent retentions of various new generation preservatives (P. radiata was also treated with creosote). Whilst these stakes were exposed at 11 sites in New Zealand (NZ) and 2...
R N Wakeling, A P Singh


Natural durability transfer from sawmill residues of white cypress (Callitris glaucophylla). - Part 2: Laboratory fungal bioassays
2000 - IRG/WP 00-20204
Extracts from sawmill residues of the naturally durable white cypress, Callitris glaucophylla were tested for fungicidal activity in a series of laboratory bioassays. The effects of different extraction solvents, techniques and sources of material on the biocidal efficacy of the resultant extracts were evaluated. Soil jar decay tests were used initially however, contractual time constraints necess...
M A Powell, L M Stephens, L Francis, M J Kennedy


Enzyme systems of bacterial isolates from ponded logs - Potentials of pectin and/or starch degradation
2000 - IRG/WP 00-10378
This paper deals with the degradation potentials of wood constituents by the bacterial isolates from ponded logs. The potentials to degrade pectin as a constituent of pit-tori as well as starch existing in ray parenchyma cells in the areas of sap- and transition wood with the isolates were examined. The pectinase activity was investigated by means of the degradation degree of a carrot strip used a...
S Doi, S Ohta


Occurrence, prevention and repair of Dry Rot
1990 - IRG/WP 1439
Information about the frequency, detection, identification and repair of Dry Rot attacks (Serpula lacrymans) is summarized from 13 European countries. Based on 28 completed and returned questionnaires it appears that Dry Rot attacks are recognized in all countries participating, and that there is an appreciable similarity in the frequency of attacks and methods of detection and identification. How...
A P Koch


The effect of hemicellulose degradation on the mechanical properties of wood during brown rot decay
2001 - IRG/WP 01-20219
Incipient decay of wood by brown rot fungi causes measurable strength losses in wood before measurable weight loss occurs. Previous studies have shown that the high levels of strength loss that occur during incipient brown rot decay may be related to loss in hemicellulose. This paper investigates the effect of decay on hemicellulose composition and the relationship to the mechanical properties of ...
S F Curling, C A Clausen, J E Winandy


Variation in infection rates of blue-stain, mould and white rot tropical fungi on mixed light Malaysian woods
2000 - IRG/WP 00-10334
The modified 3-week FRIM laboratory method for screening of anti-sapstain formulations against three representative tropical fungi causing blue-stain (Botryodiplodia theobromae), mould (Paecilomyces variotii) and white rot (Schizophyllum commune) infection of sapwood species was used to examine the relative resistance of the sapwood of eight mixed light Malaysian woods, Scots pine (Pinus sylvestri...
A H H Wong, S Ahmad


Evaluation of fungal remediation of creosote treated wood
1998 - IRG/WP 98-50101-25
Biotechnological remediation of creosote treated wood may be of interest in connection with novel recycling processes. White rot fungi and/or their ligninolytic enzyme systems are supposed to be valuable tools for such processes. This paper reports about results achieved when creosoted wood was treated in solid substrate fermentation with selected white rot fungi after different extraction procedu...
K Messner, S Böhmer


Decay and mold resistance of borate modified oriented strandboard
2003 - IRG/WP 03-40260
Decay and mold resistance of zinc borate (ZB) and calcium borate (CB) modified oriented strandboard (OSB) from southern mixed hardwoods and yellow pine was investigated in this study. Tests were done with brown rot, Gloeophyllum trabeum, and white-rot, Trametes versicolar, fungi for 8 and 12 weeks, respectively. Wood species and fungus type had significant influence on the decay resistance. Decay...
Q Wu, S Lee, J P Jones


A new method for the study of microbiological decay of wood in a strictly anaerobic environment
1988 - IRG/WP 2319
Recent work has suggested that strictly anaerobic bacteria may play a role in the biodeterioration of wood, particularly in aqueous environments. However, no suitable test methods exist for the investigation of this hypothesis. An aqueous minimal medium in specifically adapted conical flasks was sterilised, gassed using a 5.5% H2; 31.8% C02; 62.7% N2 gas mixture to remove oxygen and transferred to...
G M Rogers, A A W Baecker


The effect of tunicamycin on production and secretion of extracellular carbohydrate-degrading enzymes by Postia placenta
1988 - IRG/WP 1342
The extracellular carbohydrate-degrading enzymes of wood-decay fungi are usually heavily glycosylated and therefore stable under most denaturing conditions. It is unlikely that wood decay can be prevented by simply inactivating these enzymes. Tunicamycin, an antibiotic produced by Streptomyces lysosuperificus, prevents the glycosylation of glycoproteins and can interfere with the secretion of thes...
J A Micales, T L Highley


Moisture requirements and wood degradation of pine and spruce wood by some Basidiomycetes-fungi
1989 - IRG/WP 1406
The minimum moisture requirements for the growth of the brown rot fungi, Coniophora puteana, Gloeophyllum trabeum and Serpula lacrymans were near or above the fibre saturation point of pine and spruce, sap and heartwood, during 160 days incubation according to the standard method EN 113. The moisture of wood for the optimum growth of Coniophora puteana and Serpula lacrymans was about 35-80% and fo...
H Viitanen, A-C Ritschkoff


Experiences from a Danish large scale test by means of a new method of treatment by attack of true dry rot fungus (Serpula lacrymans) in buildings
1994 - IRG/WP 94-10064
Experiences from a new and epoch-making method of treatment in connection with the repair of attack of the true dry rot fungus, Serpula lacrymans (Fr.) S.F. Gray, are described. The paper presents the background of a large scale test comprising repair of more than 150 Danish buildings over a period of approximate 5 years. The method being both gentle to the building and presenting savings of at le...
O Munck, H Sundberg


Changes in pore structure and cell wall volume in wood decayed by brown- and white-rot fungi
1991 - IRG/WP 1501
Sweetgum (Liquidambar styraciflua L.) wood blocks were decayed by Postia (=Poria) placenta or Phanerochaete chrysosporium in soil-block cultures. Decay was terminated at various weight losses, and the pore volumes available to probes of various molecular weight and diameter were determined by the solute exclusion technique (Stone, J.E. and A.M. Scallan. 1968. Cellulose Chem. Technol. 2, 343-358.)....
D S Flournoy


Preliminary studies of the decay mechanism of some brown-rot fungi
1989 - IRG/WP 1402
The importance of the enzymatic degradation of hemicellulose and cellulose by brown-rot fungi is still under discussion. Endo-ß-1,4-xylanase and endoglucanase activities of Coniophora puteana and Poria placenta cultures were measured by the increase in reducing groups. Enzymes were produced in liquid and solid sawdust based culture media. Enzyme activities were measured in two, four and eight wee...
A-C Ritschkoff, H Viitanen


The growth and behaviour of Australian isolates of dry rot fungi, mainly Serpula lacrymans (Schum. ex Fr.) S.F. Gray. A comparision with the world literature
1983 - IRG/WP 1190
In Australia Serpula lacrymans occupies a rather unique environment, being restricted to growth within basal regions of buildings containing masonry. Its rate of timber attack in these situations appears to be far faster than that experienced in other parts of the world. Therefore the question arises as to how Australian isolates (particularly 16508 which has become a "standard" test fungus for so...
J D Thornton, O Collett


Decay resistance of Siberian larch wood against brown rot fungi - Part 2. The effect of genetic variation
1998 - IRG/WP 98-10287
Wood natural decay resistance is mainly restricted in the heartwood part of tree and based on wood microstructure and chemical composition. However, the variation of decay resistant in different wood species is vide and the effect of genotype on decay resistance of Siberian larch wood grown in Finland is not known. The aim of this work was to find out the variation on decay resistance of Siberian ...
H Viitanen, L Paajanen, T Nikkanen, P Velling


Biochemical aspects of white-rot and brown-rot decay
1987 - IRG/WP 1319
This paper presents an overview of the decomposition of wood by white- and brown-rot fungi - the most important and potent of known wood-decay fungi. These organisms are unique among cellulose destroyers because of their strong capability to enzymatically degrade lignified material. Special emphasis is given to the following aspects of wood decomposition by white- and brown-rot fungi: (1) effects...
T L Highley


The critical moisture and temperature conditions for the growth of some mould fungi and the brown rot fungus Coniophora puteana on wood
1988 - IRG/WP 1369
The growth of some mould fungi on pine and spruce sapwood was studied in 40 different constant moisture and temperature conditions. The temperature range was between +10 and +40°C and the relative humidities varied from 76 to 100% relative humidity (RH). The incubation time was 12 weeks. The mixed mould inoculation used included typical fungi growing on wood: Aspergillus versicolor, Cladosporium ...
H Viitanen, L Paajanen


Preliminary studies to assess the effects of aeration and lowered humidity on the decay capacity, growth and survival of the dry rot fungus Serpula lacrymans (Wulf ex. Fr.)
1997 - IRG/WP 97-10208
Novel microcosms were used to test separately the effects of aeration and humidity on the decay capacity, linear spread and survival of Serpula lacrymans. The application of a pumped air supply resulted in an effective cessation of fungal activity when all but the lowest of the air flow rates was used. Furthermore, the lowest air flow rate caused marked growth tropisms away from the stress. In sep...
G A Low, J W Palfreyman, N A White, H J Staines, A Bruce


Serpula lacrymans, The Dry Rot Fungus and its Tolerance towards Copper-based Wood Preservatives
2005 - IRG/WP 05-10555
Serpula lacrymans (Wulfen : Fries) Schröter, the dry rot fungus, is considered the most economically important wood decay fungus in temperate regions of the world i.e. northern Europe, Japan and Australia. Previously copper based wood preservatives were the most commonly used preservatives for pressure treatment of wood for building constructions. Because of a suspicion about tolerance toward cop...
A C Steenkjær Hastrup, F Green III, C A Clausen, B Jensen


Ability of chitosans to limit wood decay under laboratory conditions.
2004 - IRG/WP 04-30339
Chitosans have recently been explored for controlling a number of plant pathogenic fungi and have shown some promise for controlling stain fungi on wood. The potential for using chitosans either alone or in combination with copper sulfate to protect wood from fungal decay was explored using maple veneers. Chitosan treatment reduce weight losses caused by two brown rot and one white rot fungi, howe...
M Maoz, J J Morrell


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