IRG Documents Database and Compendium


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Elimination of alternative explanations for the effect of iron on treated wood
1993 - IRG/WP 93-30006
Amounts of iron which had previously been found in stakes removed from ground contact reduced decay of untreated wood by four brown-rot fungi. This suggested that the effect of iron may be on the preservative. Analysis of the leachates from CCA- and ACA-treated wood blocks first exposed to rusting iron, then to a brown-rot fungus, showed that the increased decay found in the laboratory for wood ex...
P I Morris, J K Ingram, D L Gent


Proposed test procedure to determine the effect of timber substrate on the effectiveness of a copper/chrome/arsenic preservative in seawater
1975 - IRG/WP 411
R A Eaton


Localization of oxalate decarboxylase in the brown-rot fungus Postia placenta
1996 - IRG/WP 96-10161
Oxalate decarboxylase, the enzyme that breaks oxalic acid down into formic acid and carbon dioxide, was recently detected in mycelial extracts of the brown-rot fungus Postia placenta. Differential centrifugation was used to demonstrate that the enzyme is loosely associated with the hyphal surface. Enzyme activity can be removed by washing the hyphae with a low pH buffer. Only low levels of activit...
J A Micales


Non-enzymatic Gloeophyllum trabeum decay mechanisms: Further study
2001 - IRG/WP 01-10395
Information will be presented on the mechanisms involved in, and potential application of, non-enzymatic wood decay by brown rot decay fungi. Specifically, the hypothesized role of low molecular weight phenolate derivatives will be discussed in relation to non-enzymatic degradation of wood. The mechanism of binding of iron by cellulose, and binding and reduction of iron by fungal derivatives and m...
B Goodell, J Jellison


Susceptibility of angiosperm sapwood to white-rot fungal colonization and subsequent degradation: a hypothesis
1997 - IRG/WP 97-10211
It has long been recognized that angiosperm sapwood in nature is relatively easily and preferentially degraded by white-rot fungi. This susceptibility to white-rot fungi is generally believed to be mainly caused by the structure and concentration of angiosperm lignin. However, an explicit explanation as to why lignin structure makes a particular wood vulnerable to white-rot colonisation and subseq...
T Schultz, D D Nicholas


The potential of 2-deoxy-D-glucose as an active ingredient in wood preservation
1999 - IRG/WP 99-30205
2-deoxy-D-glucose (2-DOG) is a potential active ingredient against wood decaying fungi. When dissolved in water, it can be used in pressure treatment of wood. Thereby the wood is protected from attack by wood decaying fungi. A concentration of 1.5% (mean retention 9.7 kg/cubic meter sap-wood) is adequate for brown rot fungi, and 3% (mean retention 19.5 kg/cubic meter sapwood) will also provide protection against white rot fungi. 2-DOG is easily soluble in water, and is therefore easily leached from the wood upon completion of the preservation process. Different types of fixa-tion methods have been tried and evaluated. It is possible to produce 2-DOG by the hydrolysis of chitin, a constituent of the exterior skeleton of shellfish and insects. There is a potential for exploita-tion of this waste product provided by the crab and shrimp industry. The yield of 1 kg of fresh shrimp is 75 g of 2-DOG.
O V Frederiksen, A P Koch


An insight into brown rot decay of timber as revealed by 13C CP/MAS NMR
1985 - IRG/WP 1259
L E Leightley


Monographic card: Coniophora puteana (Schum. ex Fr.) Karst. (Second draft)
1978 - IRG/WP 171
A Käärik


Studies on the biological improvement of permeability in New Zealand grown Douglas fir
1983 - IRG/WP 3231
This report outlines progress towards optimizing conditions for water storage of New Zealand grown Douglas fir with the aim of improving permeability to water-borne preservatives, in particular CCA. Small scale laboratory tests are in progress but the need to scale up to potential commercial applications is being considered. Mixed populations of bacteria isolated from 10 week water sprinkled Dougl...
K J Archer


Conditions for basidiospore production in the brown rot fungus Gloeophyllum separium in axenic culture
1984 - IRG/WP 1232
Attempts to control and optimize the production of hymenial structures and basidiospore production in Gloeophyllum sepiarium in axenic culture resulted in the proposal of the following conditions as being suitable. The dikaryotic mycelia originally isolated from basidiocarps could consistently be induced to produce hymenial structures and pure basidiospore collects if illuminated by near ultraviol...
J Bjurman


Effect of methylene bisthiocyanate on morphology and ultra-structure of a sapstain fungus, Ophiostoma floccosum
2003 - IRG/WP 03-10471
In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultrastructure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). Differential interference contrast microscopy suggested that MBT caused rapid changes to the morphology of O. floccosum resulting in excessive formation of vacuoles and...
T Singh, B Kreber, R N Wakeling, A Stewart


The true dry rot fungus (Serpula lacrymans) found in the wild in the forests of the Himalayas
1993 - IRG/WP 93-10002
J Bech-Andersen, S A Elborne, F Goldie, J Singh, B Walker


The isolation of actinomycetes from wood in ground contact and the sea
1980 - IRG/WP 1110
M S Cavalcante, R A Eaton


Detection of incipient brown rot decay in wood by fourier transform infrared spectrometry
1987 - IRG/WP 2275
Fourier transform infrared spectrometry was evaluated as a possible method for detecting different levels of brown-rot decay in pine sapwood. By using first derivative spectra and regression analysis, an equation based on 4 absorption peaks, normalized with an internal standard peak, was developed. The correlation between weight loss and absorption was highly significant indicating that this is a ...
D D Nicholas, T Schultz


Effects of the anaerobic wood decay bacterium Clostridium xylanolyticum on unbleached Pinus and Eucalyptus pulp
1991 - IRG/WP 1506
Clostridium xylanolyticum has been shown to produce extracellular enzymes capable of degrading wood. The present work was conducted to quantify growth on various lignocellulosic substrates and degradation of pulped wood fibre. In the latter tests Clostridium xylanolyticum was incubated at 35°C under anaerobic conditions in a medium containing 0.2% (w/v) peptone and 4% (w/v) unbleached Pinus and E...
G D Shelver, U Matai, W Van Wyk, A A W Baecker


Examination of timbers from the Mary Rose in storage
1988 - IRG/WP 4149
Timbers from the Tudor ship, Mary Rose, have been examined for the presence of fungal fruiting structures. Wood specimens were also examined for the presence of microbial decay patterns using light and scanning electron microscopy. Structural timbers were surveyed using the Pilodyn for an overall picture of the extent of decay. Mary Rose timbers were predominantly inhabited by marine fungi. Fourte...
R Mouzouras


Final draft of a monographic card for Pole Fungus A, L Harmsen, Rigidoporus vitreus (Fr.) Donk according to the 'Model Questionnaire for preparation of monographic cards for wood-destroying fungi'
1978 - IRG/WP 178
L Harmsen


Effects of some essential oils on wood destroying organisms
1993 - IRG/WP 93-10047
Three wood destroying fungi: Botryodiplodia theobromae Pat. (stain), Gloeophyllum sepiarium (brown rot), and Pycnoporus sanguineus (white rot) were exposed to six plant essential oils: the peppermint, kaffir lime or leech lime, lavender, tarragon, holy basil, and the eucalyptus. The peppermint oil showed most effective to inhibit fungal growth, while eucalypus oil was the least effective. The othe...
K Atisongkroh, C Anantachoke, P Lekuthai, S Pensook, T Kittirattrakarn


Methane emission by termites, Coptotermes formosanus Shiraki
1995 - IRG/WP 95-10099
Association of methanogenic bacteria only with the smallest-sized symbiotic protozoa Spirotrichonympha leidyi Koidzumi was evidenced by epifluorescence microscopic observations. Workers, which were collected from a laboratory colony and placed in a test container with water supply emitted methane at a relatively constant rate with a peak of 0.76 nmol/termite/hr within the first 72 hrs after the in...
K Tsunoda, W Ohmura, M Tokoro, T Yoshimura


Basidiosporogenesis by brown-rot basidiomycetes in vitro
1995 - IRG/WP 95-10126
Basidiospores of wood-rotting basidiomycetes are a primary sourse of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia car...
S C Croan


Use of Transverse Compression Properties as a Measurement of Wood Biodeterioration, Part 1 of 2 - Effect of White-Rot on Yellow-Poplar
2002 - IRG/WP 02-40239
The soil block and agar block test methods are used extensively as a screening process for potential and modified wood preservatives. The extent of decay in standard screening tests, indicative of preservative efficacy, is currently based on mass loss. Mass loss techniques, due to their limit of sensitivity in detecting significant mass loss vis-à-vis decay, require an extended fungus exposure ...
S Janzen, D D Nicholas


Bacteria and wood. A review of the literature relating to the presence, action and interaction of bacteria in wood
1971 - IRG/WP 101
S E Rossell, E G M Abbot, J F Levy


The dry rot fungus and other fungi in houses. Part 3
1994 - IRG/WP 94-10083
J Bech-Andersen


Monographic information on Serpula (Merulius) lacrymans (Schum. ex Fr.) S. F. Gray, according to the 'Model Questionnaire for preparation of monographic cards for wood-destroying fungi'
1978 - IRG/WP 170
J Segmüller, O Wälchli


Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner


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