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Wood and filter paper degradation, phenol oxidase and one-electron oxidation activities by the white rot fungus Ceriporiopsis subvermispora
2003 - IRG/WP 03-10486
The activities of one-electron oxidation and phenol oxidase during incubation of cultures of the white-rot basidiomycete Ceriporiopsis subvermispora containing either glucose or wood were periodically measured. Further, the degradation activities against wood and filter paper were examined during the course of cultivation. Weight losses of Japanese beech wood and Japanese cedar wood after 12 wee...
H Tanaka, S Itakura, A Enoki


Phenol oxidase activity and one-electron oxidation activity in wood degradation by soft-rot deuteromycetes
2007 - IRG/WP 07-10615
Wood degradation, one-electron oxidation activity as assayed by ethylene generation from 2-keto-4-thiomethylbutyric acid (KTBA), and phenol oxidase activity were measured in cultures of six deuteromyce fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing...
H Tanaka, M Yamakawa, S Itakura, A Enoki


Extracellular substance from the white rot basidiomycete Irpex lacteus involved in wood degradation
1992 - IRG/WP 92-1571
Cellulases, phenol oxidase, and a substance that both produced and reduced H2O2 were isolated from cultures containing wood or glucose on which the white-rot fungus Irpex lacteus was growing. The rate of wood degradation by the fungus with different amounts of glucose in the medium was measured. More of the substance that produced and reduced H2O2 was found extracellularly in cultures containing w...
H Tanaka, T Hirano, G Fuse, A Enoki


Relationship between degradation of wood, cellulose or lignin-related compounds and production of hydroxyl radical or accumulation of oxalic acid in cultures of brown-rot fungi
1994 - IRG/WP 94-10062
The degradation activities of brown rot fungi against wood, cellulose, and lignin-related compounds were measured in cultures containing glucose or wood as a carbon source. Also the activities of one-electron oxidation and hydroxyl radical production and the amount of oxalic acid present in the cultures were measured. The degradation activities of the fungi against wood, crystalline cellulose and ...
S Itakura, T Hirano, H Tanaka, A Enoki


Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse


The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Fungicidal activity of some organic solvents, copper carboxylates and their complexes with 2-aminoethanol
1997 - IRG/WP 97-30136
We evaluated the activity of eight organic solvents against wood - rotting fungus Trametes versicolor in order to choose the most appropriate one for rapid screening tests of some copper(II) carboxylates and their adducts with 2-aminoethanol. Their activity against the selected fungus was classified in the following order: chloroform > N,N-dimethylformamide > acetonitrile > methanol > ...
M Petric, F Pohleven


Resistance of Alstonia scholaris vestures to degradation by tunnelling bacteria
1992 - IRG/WP 92-1547
Electron microscopic examination of vessels and fibre-tracheids in the wood of Alstonia scholaris exposed to tunnelling bacteria (TB) in a liquid culture showed degradation of all areas of the secondary wall. The highly lignified middle lamella was also degraded in advanced stages of TB attack. However, vestured pit membranes and vestures appeared to be resistant to degradation by TB even when oth...
A P Singh, T Nilsson, G F Daniel


The dry rot fungus and other fungi in houses. Part 2
1993 - IRG/WP 93-10001
J Bech-Andersen


Electron microscopic detection and chemical analysis of three-lamellar structures in wood-destroying fungi
1984 - IRG/WP 1240
In the course of transmission electron microscopical investigations of pine wood decay by various brown- and white-rot fungi extracellular three-lamellar structures (TLS) formed by the fungi were found in specimens stained with ruthenium red. These structures occured in the lumen of the wood cell surrounding the hypha at the outermost layer of the fungal cell wall. In the course of the investigati...
R Foisner, K Messner, H Stachelberger, M Röhr


Temperature influence on the growing velocity and cellulolytic activities of Poria placenta strains from several locations
1986 - IRG/WP 2263
The differences observed on the FPRL 280 Poria Placenta strain at several Research European Laboratories for determining up the fungicide effectiveness of wood preservative has carry us to do a comparative study about the cellulolytic activity and growth velocity of each of this strains at different temperatures (22, 24 and 28°C). The results show significative differences when the temperature is...
A M Navarrete, M T De Troya


Questionnaire for Volume 2 of the basidiomycete monographs
1985 - IRG/WP 1254
12 monographs of wood destroying basidiomycetes were published in volume 1. Volume 2 includes the following 17 basidiomycetes: Antrodia serialis, Chondrostereum purpureum, Climacocystic borealis, Fomitopsis pinicola, Hyphoderma tenue, Lentinus degener, Lentinus squarrulosus, Paxillus panuoides, Phellinus contiguus, Poria xantha, Pycnoporus sanguineus, Rigidoporus vitreus, Serpula himantoides, Sistotrema brinkmannii, Stereum sanguinolentum, Trametes corrugata
T Nilsson


Ultrastructural observations on wood-degrading erosion bacteria
1986 - IRG/WP 1283
G F Daniel, T Nilsson


Some tests on ES - AS 11, a novel anti-sapstain formulation, and its properties
1987 - IRG/WP 3399
The results of some tests with the formulation ES - AS 11 are given. The formulation is an attempt to improve the performance of an anti-sapstain chemical by: 1) increasing its penetrability 2) uniquely combining its active ingredients. Very short times of treatment (dipping not longer than 5 seconds), low concentrations of active ingredients, and lower toxicological and environmental risks may be...
U Straetmans


The development of a screening method for the activity of pyrethroids against wood boring marine crustaceans, Limnoria spp
1978 - IRG/WP 443
The present work is concerned with the develepment of a suitable bio-assay technique to determine the biological activity (contact action) of pyrethroids against Limnoria spp. Estimates of the toxicity of three pyrethroids, permethrin, cypermethrin and decamethrin (the structures of which are shown in Fig. 1.) to the marine borer have been obtained....
D Rutherford, R C Reay, M G Ford


Comparative studies on the distribution of lignin and CCA elements in birch using electron microscopic X-ray microanalysis
1987 - IRG/WP 1328
The microdistribution of metal preservatives in treated wood has received considerable research over the last two decades. Despite this, little effort seems to have been made to try and correlate the distribution of CCA elements with respect to naturally occurring wood cell wall components in wood. In the present preliminary study an attempt is made to relate the distribution of lignin in-situ wit...
G F Daniel, T Nilsson


Localization of oxalate decarboxylase in the brown-rot fungus Postia placenta
1996 - IRG/WP 96-10161
Oxalate decarboxylase, the enzyme that breaks oxalic acid down into formic acid and carbon dioxide, was recently detected in mycelial extracts of the brown-rot fungus Postia placenta. Differential centrifugation was used to demonstrate that the enzyme is loosely associated with the hyphal surface. Enzyme activity can be removed by washing the hyphae with a low pH buffer. Only low levels of activit...
J A Micales


Studies on the biological improvement of permeability in New Zealand grown Douglas fir
1983 - IRG/WP 3231
This report outlines progress towards optimizing conditions for water storage of New Zealand grown Douglas fir with the aim of improving permeability to water-borne preservatives, in particular CCA. Small scale laboratory tests are in progress but the need to scale up to potential commercial applications is being considered. Mixed populations of bacteria isolated from 10 week water sprinkled Dougl...
K J Archer


Differences in feeding activity among colonies of Formosan subterranean termite Coptotermes formosanus Shiraki
1983 - IRG/WP 1202
Feeding activities of 7 colonies of the Formosan subterranean termite, Coptotermes formosanus Shiraki, were examined. Wood-consumption rates among colonies differed significantly, ranging from 23.80-78.48 mg/g/day. This large intraspecific variation raised a question of whether differences in feeding activity reported for other termite species were due to interspecific differences. When rates were...
N-Y Su, J P La Fage


Report of activity of CEN/TC 38: Test Methods for Wood Preservatives
1987 - IRG/WP 2287
G Castan


Antifungal activity of a stilbene glucoside from the bark of Picea glehnii
2001 - IRG/WP 01-10402
Stilbene glucosides are widely distributed as phenolic extractives in the bark of Picea glehnii, a commercially species planted in the northern area of Japan, and its content reaches to more than 10% by the dried weight of the bark. Although antifungal activities of these compounds have been reported, the mechanism of growth inhibition is still unclear. Isorhapontin (5,4'-dihydroxy-3&...
S Shibutani, M Samejima


Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
1988 - IRG/WP 1343
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to...
E Srebotnik, K Messner


Fungicidal properties of boron containing preservative Borosol 9
2004 - IRG/WP 04-30348
The fungicidal properties of new boron containing wood preservative Borosol 9 is described in this paper. These properties were of particular interest as this new boron containing preservative, exhibit very good performance against wood damaging insects. But because the tested boron formulation contain also nitrogen compounds, we wanted to verify if nitrogen as a nutrient could promote...
G Babuder, M Petric, F Cadež, M Humar, F Pohleven


SEM of wood dust particles
1997 - IRG/WP 97-50084
Dust particles from beech and oak trees have been classified in Germany as being "working materials which are definitely carcinogenic to humans". All other wood dusts, including those from softwoods, are classified as being materials "with reasonable suspicion of carcinogenic potential". The carcinogenic principle of action continues to remain unclear. The mechanical irritation is one of a number ...
U Schmitt, R-D Peek, A O Rapp


The effect of low molecular weight chelators on iron chelation and free radical generation as studied by ESR measurement
2000 - IRG/WP 00-10367
The focus of this work was to improve our current knowledge of the non-enzymatic mechanisms involved in brown-rot decay. Electron Spin Resonance (ESR), also known as Electron Paramagnetic Resonance (EPR), is an attractive technique for the identification and study of chemical species containing unpaired electrons (such as radicals and certain transition metal species). ESR spin-trapping techniques...
Yuhui Qian, B Goodell


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