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Molecular characterization of M’jej, decaying agent of cedar forests in Morocco
2006 - IRG/WP 06-10593
Cedar wood is well appreciated since thousand years even though its economical importance is limited because of its world distribution restricted to some Mediterranean countries and Himalaya. The most important species, Cedrus atlantica have its biggest population in Morocco (130 000 ha) where it is submitted to fungal diseases. Among them, the locally named “M’jej” reduced notably saw mile ...
A Zaremski, S Bakkali-Yakhlef, C Chaintreuil, Y Abbas, Y Prin, M Abourouh, M Ducousso


Detecting fungal DNA in treated and non-treated wood
2007 - IRG/WP 07-10621
Isolating fungi from wood has long involved culturing on selective media followed by identification using various keys. This process can be cumbersome, costly, and, most importantly, not always capable of detecting all of the fungi present. The recent development of molecular methods for isolation and identification of fungi has created tremendous opportunities for expanding our knowledge of the...
C Freitag, M Freitag, J Morrell


Molecular methods as a tool within the field of wood protection – available methods and new possibilities
2007 - IRG/WP 07-20361
The area of wood protection is in a period of change. New tools are needed to understand the mode of action, and to further improve the new wood protection systems. A set of useful tools are found among the molecular methods. This paper presents an overview of some of the tools available, and the methods are exemplified by papers within the frame of wood protection issues. However, there is stil...
G Alfredsen, A M Hietala, C G Fossdal, H Solheim


Antifungal activity of different molecular weights of a biopolymer chitosan against wood decay fungi
2008 - IRG/WP 08-30456
In recent years chitosan has been investigated as a natural polymer for wood preservation against fungal decay. From an environmental point of view, chitosan seems to possess a potential approaches as wood protecting agent. In this study, three different molecular weights of chitosan compounds (with approximately the same degree of deacetylation 83 ± 2 %) were evaluated as in vitro and in vivo as...
A S O Mohareb, M E I Badawy


Molecular Methods: a Reliable Tool for the Identification of Wood Decay Fungi in Construction Timber
2008 - IRG/WP 08-20386
In the present study, we tested the practical value of several DNA-based methods to identify at the species level the most common wood-decaying fungi infecting buildings. We successfully extracted and amplified fungal DNA from pure cultures of twelve species of wood-inhabiting fungi, from oak and pine wood infected in laboratory with known strains, and from unknown field samples of wood collected ...
M Maître, M Kutnik, I Le Bayon, L Harvengt


Wood Decay Research Using Molecular Procedures, What Can It Tell Us?
2008 - IRG/WP 08-10678
There are many unanswered questions in wood decay and protection research, and no single research technique will ever answer all of these questions. The process of wood decay is a very complex series of biochemical and chemical reactions that are heavily influenced by the hundreds of bacterial and fungal species found on environmental samples of wood. There are a variety of molecular techniques b...
S V Diehl, M. L Prewitt, Young-Min Kang, L Mangum, J D Tang


Degradation of wood veneers by Fenton’s reagents: effects of low molecular weight phenolic compounds on hydrogen peroxide decay and tensile strength loss
2009 - IRG/WP 09-20400
Pine wood (Pinus sylvestris) veneers strips were incubated in acetate buffer containing hydrogen peroxide and iron to mimic mechanisms of brown rot decay and assess the degradation of cellulose through analysis of the tensile properties of the decayed wood. The tensile properties of thin wood strips treated with Fenton system reagents or precursors were determined and correlated to weight loss as ...
Yanjun Xie, R Well, Zefang Xiao, B Goodell, J Jellison, H Militz, C Mai


Fungal Attack on Lignin and Cellulose: Elucidation of Brown- and White-Rot Mechanisms Comparing Biomimetic and In-Vivo Degradation Patterns
2010 - IRG/WP 10-10714
This paper examines research and hypotheses that have been developed over several years on wood degradation mechanisms. This information is combined with new data and analyses to explain why wood decay patterns caused by brown-rot fungi and specific types of white-rot fungi are different. New data, including work with both biomimetic studies on low molecular weight compounds, degradative enzymes, ...
V Arantes, B Goodell, A M F Milagres, Yuhui Qian, T Filley, J Jellison, S Kelley


Estimating the age of subterranean termite attack and damage in buildings
2010 - IRG/WP 10-10727
This paper describes physical and experimental methods and a mathematical model (Rotramel 2003) to assist in estimating the age of subterranean termite attack and damage to building structures. Timber, building and termite management industries have to develop new strategies to assess and estimate termite attack and damage in the current climate of global warming. A case study will be used as an e...
J R J French, B M Ahmed Shiday, J Thorpe


Profiling fungal community in wood decay ecosystem by Denaturing High-Performance Liquid Chromatography
2010 - IRG/WP 10-20443
A DNA-based fingerprinting technique, Denaturing High Performance Liquid Chromatography (DHPLC) was developed to profile fungal communities colonizing indoor timber. Molecular fungal diversity was assessed using amplification based on the Internal Transcribed Spacer (ITS1) rDNA of 74 wood samples collected from infected buildings in France. Due to its high sensitivity, the PCR-DHPLC technique was ...
S Maurice, G Le Floch, M Le Bras-Quéré, J P Rioult, G Barbier


Photostabilization of Wood with Higher Molecular Weight UV Absorbers
2010 - IRG/WP 10-30524
Higher molecular weight UV absorbers were created by reacting the epoxy-functionalized UV absorber 2-hydroxy-4(2,3-epoxypropoxy)-benzophenone (HEPBP) with maleic, phthalic or succinic anhydride. The ability of the UV absorbers to photostabilize wood was then examined. FTIR-spectroscopy confirmed that HEPBP reacted with phthalic anhydride to create a polyester that preserved the UV-absorbing benzop...
P D Evans, M J Chowdhury


TMT–Interlab–Test to establish suitable quality control techniques - Structure and first results
2010 - IRG/WP 10-40503
As a result of growing quality demands from manufacturers and end-consumers of thermally modified timber (TMT) recent scientific research activities are increasingly focussed on modification mechanisms and by this on the development of suitable quality control methods. To deepen the knowledge in modification mechanisms and to obtain a larger variety of scientific data for TMT depending on the trea...
C R Welzbacher


Molecular investigation of Postia placenta growing in modified wood
2011 - IRG/WP 11-10756
Brown rot is the most common and destructive type of fungal decay for wood in service. These fungi depolymerize preferentially the structural carbohydrates, cellulose and hemicellulose in the cell wall leaving oxidized lignin behind. Modified wood can provide protection against a variety of wood deteriorating organisms, including decay fungi. However, there is still little known about the mode of ...
B Schmöllerl, G Alfredsen, C G Fossdal, M Westin, A Steitz


Design, synthesis, characterisation and effectiveness of ‘Locked-in-Boron’ chemicals for H3.2 level of wood protection
2011 - IRG/WP 11-30577
Boratrane molecules with five-membered and six-membered molecular ring systems, and with various ring substituents were synthesised, characterised and formulated for wood treatment for accelerated laboratory bioassays using wood decay fungi as test organisms. Six-membered ring boratranes showed lower efficacy in laboratory assays than five-membered ring boratranes. One alkyl-substituted boratran...
R Franich, H Kroese, S Gallagher, S Hill, B Kelly, G Billett, R Meder, W Rae


Smart hydrogels from low molecular weight amphiphilic compounds: toward a solution to decrease leachability and increase efficacy of boron preservatives?
2012 - IRG/WP 12-30589
A new catanionic system associating amphiphilic carnosine (AlaHisC8) and lauric acid forms supramolecular hydrogel at very low concentration. This gel was investigated and we have checked the validity of the concept of hydrogels utilization to reduce boron leachability and to develop new wood protection treatments. Impregnation with 5% aqueous borax solution (w/w) and 0.3% gelator agent (...
F Obounou Akong, P Gérardin, C Gérardin-Charbonnier


Hydrogels: a solution to reduce boron leachability without reduction of its biodisponibility to wood decaying fungi?
2012 - IRG/WP 12-30602
Products used today for wood protection must fulfill to more and more environmental constraints, such as being of low toxicity in answer to the Biocidal Product Directory, but also to involve waterborne treatments to limit rejection of volatile organic compounds in the atmosphere. Boron preservatives have been described as valuable alternatives for wood protection for non-ground contact applicatio...
F Obounou Akong, P Gérardin, M-F Thévenon, S Parant, C Gérardin-Charbonnier


State of progress of utilisation of supramolecular gels for formulations of water-soluble wood preservation salts
2013 - IRG/WP 13-30630
This article is a compilation of the work done so far concerning the utilization of supramolecular hydrogels, built on low-molecular-weight amphiphilic molecules and containing boron salts conferring fungicidal properties. Mixing boron with thermoreversible hydrogels allows the formation of a supra molecular network incorporating boron and important amount of water upon gelification of the solutio...
F Obounou Akong, P Gérardin, M-F Thévenon, C Gérardin-Charbonnier


Molecular characterization and biodiversity of wood-decaying fungi in French Guiana
2014 - IRG/WP 14-10825
Fungi from tropical regions are currently under-represented in the classification system. Indeed, difficult access to tropical forests and irregular occurrence carpophores make it complicated to study fungus species in such environments, unlike in European zones where fungal diversity and taxonomy are better known. The purpose of this work was to enhance classification by integrating new data that...
A Zaremski, L Gastonguay, C Zaremski, J Beauchene


Wood biodeterioration monitoring using molecular fluorescence
2016 - IRG/WP 16-20589
Wood is an extremely complex and heterogeneous biological material, susceptible to biodeterioration by wood-destroying organisms. This process can be evaluated by natural resistance tests or non-destructive techniques, such as molecular fluorescence. The fluorescence of wood can be attributed to the presence of fluorophores groups or extractives, as a characteristic of some species. This study aim...
E Meneses Oliveira, A Florian da Costa, J W Batista Brag


NGS analysis of fungal OTUs in Aquilaria sp. from French Guyana, Cambodia, Laos and Thailand
2017 - IRG/WP 17-50328
Aquilaria is a tree species belonging to the Thymeleaceae family. When Aquilaria sp. is injured, it produce agarwood. Agarwood is characterized by a darker wood colour than the healthy one and by a strong perfume that is much esteemed by perfumers and some oriental religious communities. The production of agarwood is presumed to depend of environmental factors, among them fungi. The aim of this wo...
C Zaremski, C Malandain, O Sibourg, C Andary, G Michaloud, M Ducousso, N Amusant, A Zaremski


The Impact of Cryopreservation Techniques and of Water Quality on the Wood Degrading Ability of Fungal Strains: Recommendations for Maintenance and Culturing Procedures
2018 - IRG/WP 18-20636
In order to establish an optimal cryopreservation method, strains of Basidiomycete and Ascomycete fungi, which are among the most frequently used for wood durability testing, were evaluated using three cryopreservation procedures at -80°C. The main objective was to prevent decrease of the fungal virulence that may occur due to repeated routine sub-culturing procedures and to optimize the reactiva...
A Stum, M Montibus, I le Bayon, M Kutnik


Molecular identification of marine borers causing wood deterioration on marine facilities in Korea
2019 - IRG/WP 19-10955
The deterioration factors of wood which was used in the marine environment, Tongyeong (South Coast) and Incheon (West Coast) in Korea, were investigated in order to develop technologies for safe and long-term use of timber in ocean facilities. The marine microorganisms isolated by damaged wood were identified as morphological Chelura sp. and Teredo sp. As a result of DNA sequencing, it was confir...
Sae-Min Yoon, Min-Ji Kim, Won-Joung Hwang, Yong-Seok Choi, Dong-Won Son


Wood protection techniques and natural weathering: their effect on aesthetics and preference of people
2019 - IRG/WP 19-50351
Current research successfully contributes to improving wood protection techniques. However, the vast majority of research on preventing degradation of wood ignores a critical aspect of making successful products and processes – user selection of materials, which may largely depend on the aesthetical qualities. Wood treatments change the tactile and visual properties of wood substantially and the...
D Lipovac, M D Burnard, A Sandak, J Sandak


Limiting Polysaccharide Motion Protects Wood From Decay
2020 - IRG/WP 20-40890
It is well known that chemical modifications to improve decay resistance also reduce the equilibrium moisture content (EMC) of wood. The mechanism of this action, however, has been the subject of much debate. Several groups have suggested that decay resistance is a result of lower diffusion rates of fungal degradation agents through the wood cell wall. A recent paper explained the fundamental prin...
C G Hunt, S L Zelinka, J E Jakes


DuraSoft Project: a multidisciplinary approach for softwood protection
2021 - IRG/WP 21-50367
Over the course of millennia, fishing and agro-pastoral activities have produced unique housing types in the Italian lagoons and in the Slovenian highlands where the use of wood is predominant. They are associated with wooden service infrastructures such as piers, moorings, piling and fences. However, the existence of these structures and the associated cultural heritage are subject to constant ...
I Guarneri, V Moschino, N Nesto, T Marceta, M Sigovini, E Borella di Torre, L Dametto, S Pasqual, M Humar, B Lesar, T Cibic, V Natali, E di Poi, F Cerino, D Formasaro, M Kralj, F Relitti, C Balestra, M Celussi, A Franzo, A Volpi Ghirardini, M Picone, G G Distefano, M Russo, D Marchetto, R Rakar, I Trobec, D Marinic, G Rep, N Thaler, T Galvan, R Lazzarini, F Gombac, D Tagliapietra


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