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The full guideline for the “double layer test method” - A field test method for determining the durability of wood out of ground
2004 - IRG/WP 04-20290
This guideline describes a test method for wood to be exposed to the weather out of ground contact. The main objective of the method is to evaluate the durability of wood in above ground exposure as shifted overlaid stakes, which form the so called double layer. The method is applicable to the testing of untreated, treated and modified wood. Initially it was developed and found to be most useful ...
A O Rapp, U Augusta


Immunolocalization of xylanase produced by Clostridium xylanolyticum Rogers and Baecker during anaerobic decay of Pinus patula
1991 - IRG/WP 1505
The xylanolytic fraction from a 72h culture of Clostridium xylanolyticum ROGERS and BAECKER was separated using non-denaturing PAGE and then used to raise polyvalent antisera in rabbits. Antibodies were purified using an affinity gel column and coniugated with colloidal gold. Pinus patula was exposed to axenic cultures of Clostridium xylanolyticum for 3 weeks, fixed, embedded and converted to ultr...
A A W Baecker, G M Rogers


The effect of extent and location of decay on strength loss in constructional timber
1994 - IRG/WP 94-30039
Radiata pine heartwood billets, 50 x 100 x 1000 mm³ were end-sealed and treated with copper-chrome-arsenate preservative (NZ Type I) to a range of retentions and penetration patterns. Cross-sections, 10 mm thick, were cut from the centre of each billet and used for determination of preservative distribution, for preservative analysis and for decay tests. The decay test consisted of laying samples...
M E Hedley, H Bier


Preservative effect of cellulose insulation material against some mould fungi and brown rot fungus Coniophora puteana in pine sapwood
1991 - IRG/WP 1484
The influence of mineral wool and loose-fill cellulose wool on biodeterioration of pine sapwood was studied. The test fungi were mould fungi Aspergillus versicolor, Cladosporium sphaerospermum, Penicillium sp. and Aureobasidium pullullans and brown rot fungus Coniophora puteana. Cellulose wool inhibited the growth of mould fungi on the face of wood contacted with insulation material at RH of 97 an...
H Viitanen


Biochemical relationships between biodegradation of cellulose and formation of oxalic acid in brown-rot wood decay
1991 - IRG/WP 1472
Non-enzymic hydrolysis of cellulose with low concentrations of oxalic acid was examined. The incubation of pine wood pulp with 1% oxalic acid (pH 1.3) at 35°C for 4 weeks reduced the original viscosity to 60%. Reducing sugars were liberated from various cellulosic samples by the oxalic acid treatment. However, crystallinities of cellulose in those samples did not change before and after the treat...
M Shimada, Y Akamatsu, A Ohta, M Takahashi


Accelerated decay tests on DNBP-treated Pinus patula and Eucalyptus grandis
1987 - IRG/WP 3441
Blocks (10 x 10 x 5 mm³) of Pinus patula and Eucalyptus grandis were impregnated to 0, 1.5, 3.0 and 4.5 kg/m³ 2-sec-butyl, 4, 6 dinitrophenol (DNBP). They were monitored microscopically and for weight losses over 16 weeks against monocultures of Coriolus versicolor and Coniophora puteana. Treated ministakes (10 x 10 x 100 mm³) and blocks in soil contact were similarly monitored to determine wei...
L D Abraham, A A W Baecker


Biocidal screening method of wood extractives by a direct use of cellulose TLC plate
2001 - IRG/WP 01-20226
Most of the methods to evaluate the biological durability of woods have focused on decay fungi and termite as deteriorating organisms and it is well known that one of the most important factors affecting the biological characteristics of wood is extractives. Bioassays for evaluating the biological activities of wood extractives have been mainly conducted with treated filter papers for termites or ...
R Yusiasih, T Yoshimura, T Umezawa, Y Imamura


Decay and termite durabilities of heat-treated wood
2004 - IRG/WP 04-40272
Decay and termite resistances of Plato-treated timbers were evaluated using a modified JIS decay test method, feeding test in a laboratory scale and a field exposure against Reticulitermes speratus (Kolbe) or Coptotermes formosanus Shiraki. The results showed that no significant decay durability of the treated timbers although mass loss from decay slightly decreased in case of treated timbers. ...
S Doi, K Hanataa, E Kamonji, Yuuji Miyazaki


Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates
2003 - IRG/WP 03-10475
D-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase (glucose 2-oxidase) type wi...
T H de Koker, M D Mozuch, P J Kersten


Assessment of wood decay in small-scale unsterile soil-bed tests
1997 - IRG/WP 97-20111
This study is concerned with the investigation of wood decay in small-scale unsterile soil-bed tests. Tests were performed with untreated heartwood samples from five hardwood species of different natural durability classes ranging from highly perishable to very resistant. Our results confirmed the critical role of soil moisture content on wood decay. The highest decay rates were observed at moistu...
L Machek, A M Derksen, R Sierra-Alvarez


Preservation of robinia wood (Robinia pseudoacacia L.) stakes by vintners
1982 - IRG/WP 3194
This work discusses decay resistance of robinia wood to fungi Trametes versicolor (L.ex Fr.) Pil. and Coniophora puteana (Schum. ex Fr.) Karst., and also the possibility of influencing its resistance by means of chemical protection. The results showed that natural resistance of robinia wood to these two different agents of wood decay is not the same. It is much less resistant to the fungus Conioph...
R Benko


Effect of mini-block test conditions on activity of Coniophora puteana
2000 - IRG/WP 00-20184
The influence of various factors of the mini-block test method on growth and activity of different strains of Coniophora puteana was studied. Decay rates of spruce samples in Petri dishes of different depths, at different wood fibre orientations and at various distances between growth medium and wood were compared. In deep Petri dishes, there were no significant differences between activity of the...
F Pohleven, M Petric, J Zupin


Effect of treating process on efficacy of CCA in a laboratory decay test
1990 - IRG/WP 3628
Test samples of Pinus radiata sapwood measuring 40x40x500 mm³ were treated with a range of concentrations of the copper-chrome-arsenate (CCA) formulation "Tanalith C" using Rueping (empty cell), Lowry (empty cell) and Bethell (full cell) treatment processes. Samples were then reduced to 40x40x7 mm³ test blocks and exposed to the decay fungus Coniophora puteana using an agar/block technique. Repl...
M E Hedley, K Nasheri, J G Van der Waals


A technique for assessing the preventive efficacy against decay fungi of preservative treatments applied to wood
1988 - IRG/WP 2309
A method is described in which test blocks with envelope preservative treatments can be challenged by selected test fungi previously established on an untreated feeder block. The progress of the test fungus through the treated zone is monitored using novel baits or sensors inserted in holes drilled into blocks to within predetermined distances of the face being challenged. Preliminary results show...
J K Carey, A F Bravery


Potential toxicants for controlling soft rot in preservative treated hardwoods. Part 1: Laboratory screening tests using a filter paper technique
1977 - IRG/WP 290
Thirty-one miscellaneous formulations have been screened using a filter paper assay technique, for their efficacy in controlling the growth of Chaetomium globosum, Cephalosporium acremonium, and a mixed inoculum of finely ground soft-rotted wood obtained from preservative treated transmission poles. The formulations consisted of various organic compounds and inorganic preparations made from differ...
H Greaves


Remedial ground-line treatment of CCA poles in service. Results of chemical and microbiological analyses 6 months after treatment
1986 - IRG/WP 3388
CCA-treated poles in service with incipient internal soft rot were remedially treated by inserting borate rods, brushing with a boron/glycol solution and injecting boric acid paste, copper/creosote paste or a commercial product (DFCK paste). The spread of active chemicals in the treated zone as well as the change in microflora have been studied with time. After six months chemicals had spread to m...
B Henningsson, H Friis-Hansen, A Käärik, M-L Edlund


Quality of timber impregnated with preservatives of class AB after three years in service
2002 - IRG/WP 02-20241
Quality of impregnated timber is most often expressed by penetration and retention of the preservative used. Relatively less information is available about the performance of the final product, e.g. decks, panels etc. regarding product’s surface checks, deformations and overall appearance. Together with the timber durability, the above mention features are of esthetical importance in the above g...
N Terziev


Statistical evaluation of 'micro-bending' samples for classification of wood attacking fungi
1992 - IRG/WP 92-1544
Micro-specimens from both hardwood and softwood were exposed to selected species of field isolated fungi. The strength reduction and weight loss caused by these fungi were determined. A statistical procedure was then developed to classify the fungi into brown-, white-rot, or non-decay fungal categories based on these data. The procedure reveals the extent of hazard a fungus poses to wood members. ...
B Goodell, Jing Liu, A Homola, J Jellison, J Shottafer


Durability of five wood species against Coriolus versicolor
1992 - IRG/WP 92-1578
The durability of following five wood species Zelkova carpinifolia, Ulmus glabra, Carpinus betulus, Acer trauvetteri and Pterocarya fraxinifolia were studied against Coriolus versicolor under laboratory conditions according to DIN 52176 standards. Zelkova caprinifolia shows a reduction in weight of 2.19%, Ulmus glabra 41.69%, Carpinus betulus 45.64%, Acer trauvetteri 53.05%, and Pterocarya fraxini...
S M Kazemi


Alternatives to CCA for ground contact protection of timber: a perspective from UK on performance and service life expectations
2002 - IRG/WP 02-30289
The proposed amendment to the European Union's Marketing and Use Directive (1976/769/EEC) in respect of arsenic in CCA wood preservatives seeks to restrict the use of CCA across the European Union. CCA is an extremely important wood preservative in the UK from the manufacturing of the product to the extent of use of CCA-treated timber. Based on our experience and judgement on the use of ...
E D Suttie, A F Bravery, T B Dearling


SEM investigation of the production of extracellular mucilaginous material (ECM) by some wood inhabiting and wood decay fungi when grown on inert surfaces
1997 - IRG/WP 97-10194
A range of wood inhabiting and wood decay fungi have been studied for their production of extracellular mucilage when grown on inert surfaces, for comparison with their growth on wood. The species tested were: Coniophora puteana, Coriolus versicolor, Chaetomium globosum, Trichoderma harzianum, Ceratocystis coerulescens, Ophiostoma piceae, Mucor sp. and Penicillium sp. They were grown on plastic me...
A R Abu, R J Murphy, D J Dickinson


The practice of using concrete on wood piling for marine use in Thailand
1982 - IRG/WP 492
The practice of using concrete on wooden poles has been carried on in Thailand for a long time in pile-houses and pier constructions which have been situated in, or partly in the sea. In such instances, the hewed round and/or square-sawn heartwood poles of naturally durable timber species have been coated with concrete of about 5 to 10 cm or more in thickness, and to about 100 cm above the highest...
B Anuwongse


Oxalic acid quantification, oxaloacetase assay and ESI localization of P, C, and Fe from the brown rot fungus Postia placenta
1994 - IRG/WP 94-10063
The mechanism by which brown-rot fungi initiate depolymerization of holocellulose in wood remains unknown. Recently, oxalic acid (OA) has received considerable attention in cellulose breakdown by brown-rot fungi. The OA could serve as a proton donor for hydrolytic or an electron donor for oxidative (Fenton's reaction-H2O2/Fe2+) cleavages of cellulose. The acid may originate via oxaloaceta...
C R Jordan, W V Dashek, T L Highley


Chlorothalonil: A new ground contact wood preservative
1992 - IRG/WP 92-3712
Extensive investigations have been conducted in recent years on the performance of chlorothalonil (tetrachloroisophthalonitrile) as a ground contact wood preservative. Efficacy information is presented on a soil block test with 11 Basidiomycetes, a fungus cellar evaluation, above-ground field testing against termites in Australia and Hawaii, and ground-contact field stakes installed in Florida (9 ...
P E Laks, T L Woods


Some Experiences with Stake Tests at BAM Test Fields and in the BAM Fungus Cellar Part 2: Comparison of Static and Dynamic Moduli of Elasticity (MOE)
2005 - IRG/WP 05-20320
In routine in-ground stake tests of wood preservatives at the BAM test field Horstwalde and in the BAM fungus cellar periodical determinations of both static moduli of elasticity (MOE) on the basis of bending tests and dynamic MOE on the basis of the vibration method were performed as a possible method for the assessment of fungal attack. As expected from other publications, the absolute values of...
M Grinda, S Göller


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