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Preliminary PIXE micro-analysis of copper-chrome-arsenic preservative treated Malaysian hardwoods
1999 - IRG/WP 99-20169
The novel nuclear microscopy, essentially the Particle-induced X-ray Emission (PIXE) system, was applied for examining the macro-distribution and micro-analysis of copper-chrome-arsenic (CCA) preservative in Malaysian hardwoods Koompassia malaccensis, K. excelsa, Alstonia spp. and Dipterocarpus spp., and one temperate pinewood Pinus sylvestris, where the preservative retention differed between the...
A H H Wong, R B Pearce, G W Grime, F Watt


Extracellular substance from the white rot basidiomycete Irpex lacteus involved in wood degradation
1992 - IRG/WP 92-1571
Cellulases, phenol oxidase, and a substance that both produced and reduced H2O2 were isolated from cultures containing wood or glucose on which the white-rot fungus Irpex lacteus was growing. The rate of wood degradation by the fungus with different amounts of glucose in the medium was measured. More of the substance that produced and reduced H2O2 was found extracellularly in cultures containing w...
H Tanaka, T Hirano, G Fuse, A Enoki


Relationship between degradation of wood, cellulose or lignin-related compounds and production of hydroxyl radical or accumulation of oxalic acid in cultures of brown-rot fungi
1994 - IRG/WP 94-10062
The degradation activities of brown rot fungi against wood, cellulose, and lignin-related compounds were measured in cultures containing glucose or wood as a carbon source. Also the activities of one-electron oxidation and hydroxyl radical production and the amount of oxalic acid present in the cultures were measured. The degradation activities of the fungi against wood, crystalline cellulose and ...
S Itakura, T Hirano, H Tanaka, A Enoki


Visualising Bacteria in Wood Using Confocal Laser Scanning Microscopy (CLSM)
1998 - IRG/WP 98-10272
A fluorescent phospholipid probe was used in conjunction with confocal laser scanning microscopy (CLSM), to visualise bacteria which inhabit in radiata pine wood and degrade pit membranes. CLSM has the ability to collect fluorescent images through different emission filters at the same time, so it is possible to distinguish gram-positive and gram-negative bacteria in infected wood by counterstaini...
Ying Xiao, A P Singh, R N Wakeling


Failure of overhead power transmission and telecommunication poles in Tanzania: Causes, preventive and remedial measures
1988 - IRG/WP 3465
Unless measures are considered urgently to combat massive pole failures occuring in service, it is likely that wooden poles may soon become unpopular. Consequently, pole users may be forced to use costlier alternatives such as underground cables and concrete or steel poles. In trying to countercheck this trend, this paper names the main agents of pole failure, discusses different models of pole fa...
K K Murira


Wood decay of Pinus sylvestris L. and Fagus sylvatica L. by marine fungi. Part 2
1991 - IRG/WP 1471
The water-logged wood conservation requires a good knowledge of the wood decay organims which produce the degradation. Previous conducted studies (ESCORIAL, TROYA, GARCIA DE LOS RIOS, 1990) have shown that the incubation times normally used for in vitro laboratory tests are not sufficients for a significant wood degradation. This has been the reason why the aim of this work was the study of such d...
M T De Troya, A M Navarrete, M C Escorial


Investigation of extracellular mucilaginous material in some wood decay fungi
1996 - IRG/WP 96-10188
The external morphology of the extracellular mucilagenous material (ECM) produced by Coriolus versicolor and Coniophora puteana during colonization of Scots pine and beech was studies using SEM. Specimens were examined in the frozen hydrated, freeze-dried and critical point dried state. All technics produced artefacts but the ECM was best preserved when examined the frozen hydated state. Critical ...
A R Abu, D J Dickinson, R J Murphy


Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse


Soft rot fungi found in copper/chrome/arsenic treated hardwood power transmission poles in Queensland
1978 - IRG/WP 185
In Queensland the effective service life of copper/chrome/arsenic (CCA) treated hardwood telegraph and power transmission poles is being dramatically shortened due to attack by soft rot decay fungi. The Australian Wood Preservation and Pole Industries are funding research into this serious soft rot problem involving C.S.I.R.O. and the University of Queensland. The C.S.I.R.O. is actively engaged wi...
L E Leightley


The effect of a chelator mediated fenton system on activation of TMP fibres and decolorization of synthesized dyes
2004 - IRG/WP 04-50223
The purpose of this work is to improve our current knowledge of the non-enzymatic mechanisms involved in the brown rot degradation of wood, but also to study the potential applications of a chelator-mediated Fenton system in activation of wood fibers and decolorization of synthesized dyes. In this work, Electron Spin Resonance (ESR) spin-trapping techniques were used to study the generation of hyd...
Yuhui Qian, B Goodell, J Jellison


Hyphal tunnelling of belian (Eusideroxylon zwageri) wood cell walls
1996 - IRG/WP 96-10152
Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods. Visual inspections at the groundline of belian transmission poles from Sarawak, Malaysia, showed only surface decay of wood after 20 years in service. The cause of decay in belian was investigated by light and transmission electron microscopy. Light microscopy of transverse sections of surface tissue...
A P Singh, A H H Wong


Studies of the mechanism of chromated-copper preservative. Fixation using electron spin resonance
1992 - IRG/WP 92-3701
Two softwoods and one hardwood species were treated with chromium trioxide, copper sulphate, chromated-copper wood preservative (CCA). The treated wood samples were analyzed during fixation by electron spin resonance (ESR). ESR spectra indicated that more than one Cr(V) species was generated from Cr(VI) soon after CCA treatment. The Cr(V) signal became strong within increased several hours followe...
K Yamamoto, J N R Ruddick


Arbeitsverfahren zum Herstellen und mikroskopischen Auswerten von Dünnschnitten aus Holz-Bohrkernen. [Working method for producing and evaluating microscopically thin cuts of bore samples]
1980 - IRG/WP 2133
Bore samples are important specimens; e.g. they are in use to investigate poles in service. On the basis of known methods of microtomy a technique is described which permits the production of longitudinal cuts from bore samples. For this purpose, the bore sample is glued onto a mounting; the surface of cut is coated with a polystyrene solution. The cut thus consolidated can be stained, drained, an...
S Cymorek


Further studies on soft rot decay in CCA treated Eucalyptus power transmission poles in Queensland, Australia
1980 - IRG/WP 1115
Preliminary results on the examination of fungi isolated from CCA treated Eucalyptus power transmission poles were presented at the 10th Annual IRG Meeting. Since then further examination and identification of fungal isolates has been completed together with screening tests for wood decay ability and tolerance to creosote and CCA and its components. Chemical analyses for CCA retention have also be...
L E Leightley


Wood and filter paper degradation, phenol oxidase and one-electron oxidation activities by the white rot fungus Ceriporiopsis subvermispora
2003 - IRG/WP 03-10486
The activities of one-electron oxidation and phenol oxidase during incubation of cultures of the white-rot basidiomycete Ceriporiopsis subvermispora containing either glucose or wood were periodically measured. Further, the degradation activities against wood and filter paper were examined during the course of cultivation. Weight losses of Japanese beech wood and Japanese cedar wood after 12 wee...
H Tanaka, S Itakura, A Enoki


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


Performance of groundline bandage remedial treatments in western US species transmission poles
1993 - IRG/WP 93-30019
Groundline bandages are an important element in remedial treatment strategies for utility poles in the United States. Several bandages containing new or reformulated components have recently been developed but have not been adequately tested. This paper reports on the degree of diffusion of copper naphthenate, boron, and sodium fluoride from groundline bandages into pressuretreated Douglas-fir, po...
P G Forsyth, J J Morrell


Comparison of the anti-fungal efficacy of Cobra with drill perforation on oscillating pressure treated spruce transmission poles -Laboratory test
1988 - IRG/WP 3469
A laboratory method for measuring the depth of penetration of the fungicidal concentrations effective against basidiomycetes (brown rot) was developed. The tests on CCB oscillating pressure treated spruce transmission poles have shown that the depth of salt penetration can be increased by about 10 mm by a drill perforation before the impregnation. An even better penetration is reached, however, if...
E Graf, B Zgraggen


Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp


Minimum inhibitory concentration of methylene bisthiocyanate towards selected wood inhabiting fungi in liquid culture and on radiata pine
2001 - IRG/WP 01-30261
The objective of the current study was to determine the minimum inhibitory concentration (MIC) of methylene bisthiocyanate (MBT) towards wood inhabiting fungi common on unseasoned radiata pine (Pinus radiata D. Don). The fungi used were Ophiostoma floccosum, Leptographium procerum, Sphaeropsis sapinea and Trichoderma viride. Fungi were individually inoculated into nutrient media spiked with differ...
T Singh, B Kreber, C Chittenden, R N Wakeling, A Stewart


The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Studies and experiences of occurrence and development of soft rot in salt-treated poles of pine (Pinus sylvestris) installed in Swedish transmission lines in the years 1940-1945
1977 - IRG/WP 277
The present work describes the result of microscopic examinations of some 2,000 borings extracted from 20 to 23-year-old salt-treated poles (Scots pine) from the southern and central parts of Sweden. The purpose was to map out the occurrence of soft rot in Sweden and its influence on the mechanical strength of the poles as well as to contribute to the development of new and more satisfactory metho...
H Friis-Hansen


Biological test, AAS and EPR study of copper monoethanolamine complex with quaternary ammonium compounds as a wood preservative
2003 - IRG/WP 03-30321
Experiments were carried out on the wood preservative with a strong fungicidal activity based on Cu(II) carbonate, 2-aminoethanol (monoethanolamine) and quaternary ammonium compound (QAC). The object of the performed investigations was sapwood of Scots pine (Pinus sylvestris L.) treated with Cu-EA-QAC formulation. Mycological investigations were carried out according to EN 113 and EN 84 standards....
B Mazela, I Polus, S K Hoffmann, J Goslar


Distribution of boron from fused borate rods in Douglas-fir transmission poles
1996 - IRG/WP 96-30112
The diffusion of boron from fused borate rods (disodium octaborate tetrachydrate) was monitored over 42 months in CCA-treated Douglas-fir transmission poles. The boric acid equivalent was estimated by the curcumin/salicylic acid color test on increment cores removed from the poles. Moisture content of the poles was quite variable but was always above 20 percent. The percent of increment core lengt...
T L Highley, F Green III, W F Finney


The examination of preservative-treated radiata pine using electron spin resonance spectroscopy
1987 - IRG/WP 3423
Electron spin resonance spectroscopy (ESR) may be used to characterise the molecular environment of chemical species containing unpaired electrons. These species include free radicals, bi-radicals, point defects in solids, localised crystal imperfections and many transition metal ions. ESR has previously been applied to the examination of wood weathering or wood pyrolysis and now FRI and Massey Un...
D V Plackett, E W Ainscough, A M Brodie


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