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Neem leaves, a potential source for protection of hardwood against wood decaying fungus
2005 - IRG/WP 05-30370
Neem leaves are widely recognized for their insecticidal, pesticidal, fungistatic and fungitoxic ability. The present study is to investigate the efficacy of neem leaves extractives against wood destroying microbes, a brown and a white rot. Neem leaves extractives prepared in various solvents such as petroleum ether, acetone, ethanol, methanol and hot water were tested at a concentration levels of...
S Dhyani, S Tripathi, V K Jain


Degradation of resin constituents in various wood species by the white rot fungus Bjerkandera sp. strain BOS55
1999 - IRG/WP 99-10301
In previous studies, the white-rot fungus Bjerkandera sp. strain BOS55 was shown to cause extensive degradation of lipophilic extractives (resin) in Scots pine wood. Further research was carried out in order to investigate the ability of Bjerkandera sp. for reducing resinous constituents in various softwood (Douglas fir, larch and spruce) and hardwood species (birch, beech and poplar). The greates...
J Dorado, T A Van Beek, F W Claassen, R Sierra-Alvarez


Why don't more people work with Serpula lacrymans nowadays? A discussion of some of the different approaches to experimenting with this unique fungus
1989 - IRG/WP 1383
Remarkably few laboratories are currently investigating this well-known fungus. Not only has it failed to gain acceptance as a standard test organism (for wood preservative evaluation) in many countries, but is also sometimes excluded from pure research studies when it ought to be an obvious first choice as a representative basidiomycete. This paper discusses some different approaches that have be...
J D Thornton


Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth


Extracellular laccase production in brown-rot fungus Coniophora puteana
2001 - IRG/WP 01-10391
Coniophora puteana, one of representing brown-rot fungus, was investigated in terms of extracellular ligninolytic enzyme production. Three fungal strains of C. puteana were inoculated in liquid culture medium with sawdust of pine and oak wood. Extracellular laccase production was observed in some strains of C. puteana in the culture medium with sawdust of oak wood. The highest activity occurred at...
Kwang-Ho Lee, Seung-Gon Wi, Yoon Soo Kim


Influence of the nutritional elements on pigmentation and production of biomass of bluestain fungus Aureobasidium pullulans
1997 - IRG/WP 97-10198
The effect of the carbon source and the amount of the nitrogen on the melanization and the production of mycelial mass of bluestain fungus Aureobasidium pullulans was studied by using solid state cultivations. The carbon sources used varied from easily soluble sugars to structural polysaccharides existing in lignocellulosic material. The amount of melanin was evaluated by using partial purificatio...
A-C Ritschkoff, M Rättö, F Thomassin


Evaluation of decay fungi in Norwegian buildings
2005 - IRG/WP 05-10562
During a period of 2 years and 3 months (1 January 2001 - 20 March 2003) Mycoteam had 3161 consultations in buildings in southern Norway, 1428 revealing damage from decay fungi. One consultation often revealed several occurrences of fungi, and the total number of occurrences of decay fungi was 3434. Thirty-five different species/genera/groups of decay fungi were recorded. During this period brown ...
G Alfredsen, H Solheim, K Mohn Jenssen


The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


The role of oxalic acid in short fiber formation by the brown-rot fungus Postia placenta
1993 - IRG/WP 93-10028
The mechanism by which brown-rot fungi depolymerize wood and cellulose remains a perplexing problem. Current evidence favors oxidation by low-molecular weight, non-enzymatic fungal metabolites. During attack of cotton cellulose by Postia placenta MAD-698, the degree of polymerization (DP) decreases to limit of degree of polymerization (LODP) over a period of 5-6 weeks with relatively low weight lo...
F Green III, J M Hackney, C A Clausen, M J Larsen, T L Highley


Biological variability in the oxalate/oxalate decarboxylase system among five isolates of the wood-degrading fungus Meruliporia incrassate
2006 - IRG/WP 06-10573
The “dry-rot” wood decay fungus Meruliporia incrassata has recently attracted attention, primarily in the western coastal United States, as a particularly destructive pest of building material. Recently, the US Environmental Protection Agency (EPA) has accepted a voluntary withdrawal of the historically effective chromated-copper arsenate (CCA) as a preservative for wood used in residential se...
C Howell, J Jellison


Micromorphological Characteristics of Degradation in Bamboo Attacked by White Rot Fungus Lentinus edodes
2006 - IRG/WP 06-10575
In comparison with micromorphological works for wood decay, little known is about the decay pattern of bamboos by wood decay fungi. The present work was undertaken to understand the general degradation pattern of bamboo and the influence of polylaminate layers in bamboo fiber walls on the restriction of fungal decay. The weight loss of bamboo species Phyllostachys puberscens against the white rot ...
Chang Hyun Cho, Kwang Ho Lee, Yoon Soo Kim


Micromorphology of Bamboo Fibers Degraded by Brown-Rot Fungus Gloeophyllum trabeum
2006 - IRG/WP 06-10576
The decay pattern of bamboo by brown-rot fungus was examined. In addition, the influence of polylaminate structures in bamboo fibers on the restriction of fungal decay was also investigated. The weight loss of bamboo Phyllostachys puberscens by the brown-rot fungus Gloeophyllum trabeum after 16 week incubation was about 25%. Parenchyma cells were severely degraded. Microscopical studies showed tha...
Kwang Ho Lee, Chang Hyun Cho, Yoon Soo Kim


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Beetle-Fungus Associations in Woody Substrates in the Context of International Trade
2007 - IRG/WP 07-10610
Interest in the role of insects in transmitting various fungi and in the potential for outbreaks of insects vectoring plant disease organisms has resulted in a large volume of literature on insect-fungi relationships. Mites are mentioned as an important third partner in these associations. This paper summarizes information and provides an update on major aspects of these intricate associations in ...
A Uzunovic


Genome-wide survey of cellulase related genes of white rot fungus,Pleurotus ostreatus
2007 - IRG/WP 07-10627
A white rot fungus, Pleurotus ostreatus, which is a important edible mushroom, has received much attention to apply to bioremediation and bioconversion because it has both cellulase and ligninase. To confirm copy number of cellulase-related genes encoded in P. ostreatus genome, we attempted to genomic Southern hybridization of P. ostreatus. The draft genome sequence and a large quantity of EST and...
T Tamenori, S Horisawa


Laser stain removal of fungus-induced stains on paper-based cultural relics
2007 - IRG/WP 07-40389
Laser techniques has been developed and applied in commercial use for 40 years, but it has not been used successfully to remove stains from paper-based cultural relics . The purpose of this study was to investigate the paper cleaning by using a technique of Nd-YAG laser. Artificial stained papers were inoculated with fungi and then were treated at different laser cleaning parameters, in order to f...
Tsang-Chyi Shiah, Han Chien Lin


Effect of fiber type and content on the natural durability of wood flour/high density polyethylene composites against rainbow fungus (Coriolus versicolor)
2007 - IRG/WP 07-40387
In order to evaluate the effect of fiber type and content on the natural durability of wood flour/high density polyethylene (HDPE) composites against Coriolus versicolor, samples containing 25% and 50% by weight of various natural fibers and HDPE were selected. Natural fibers included in the study were wood flour, rice hulls, hemp fibers and newsprint. Samples containing 25% and 50% natural fiber ...
A Karimi, M Tajvidi, S Pourabbasi


Effect of Compatibilizer on the Natural Durability of Wood Flour/High Density Polyethylene Composites Against Rainbow Fungus (Coriolus versicolor)
2007 - IRG/WP 07-40388
To evaluate the effect of compatibilizer on the natural durability of wood flour/high density polyethylene composites against Coriolus versicolor, composites containing 25% and 50% by weight maple wood flour and 1% and 2% compatibilizer (Maleic anhydride polyethylene (MAPE)), respectively, were sampled. Identical specimens of the same composites without the compatibilizer were also prepared. ...
Sara Pourabbasi, A Karimi, D Parsapajouh, M Tajvidi, M Soleymani


Decay resistance of propionylated Iranian beech against the white rot fungus Tramets versicolor
2008 - IRG/WP 08-30449
In this study, the decay resistance of propionylated beech was investigated against the white rot fungus Trametes versicolor. Iranian beech (Fagus orientalis) was modified with propionic anhydride at different temperatures, concentrations and times without using any catalyst. Different weight percentage gains (WPGs) were obtained under different conditions. The decay resistance of the esterifed wo...
M R M Farahani, S M Hosseini


Biological control of the sapstain fungus Diplodia pinea (syn. Sphaeropsis sapinea) in the field
2008 - IRG/WP 08-10663
Diplodia pinea, syn. Sphaeropsis sapinea, is well known as the worldwide ocurring causal agent of the Diplodia tip-blight disease on various pine species, leading to great losses of valuable wood in the forests. Moreover, this fungus is also responsible for the fornation of sapstain of felled logs, leading to undesired disation of surface layers in the OSB manufacturing industry. In Central Europe...
C Gradinger, T Boisselet, K Messner, K Fackler


Isolation and identification of stain fungi and mould fungi on bamboo wood in China
2009 - IRG/WP 09-10703
One hundred and seventeen isolations were isolated from 150 specimens of mildewed or stained bamboo wood and bamboo products from 9 locations in north, south and central China. The molecular (ribosomal DNA Internal Transcribed Spacer, rDNA-ITS) and morphological identification results showed these fungi could be categorized 2 phyla, 2 classes, 4 subclasses, 10 orders, 15 families, 22 genera, 56 sp...
Xingxia Ma, Mingliang Jiang, Daochun Qin


Modelling hyphal growth of the bio-incising fungus Physisporinus vitreus
2010 - IRG/WP 10-10710
The white-rot fungus Physisporinus vitreus preferentially degrades the pit membranes of bordered pits in tracheids and subsequently enhances wood permeability. Thus, P. vitreus can be used to improve the uptake of wood preservatives and environmentally-benign wood modification substances. This process can be used to enhance the use and sustainability of native conifer wood species by the wood indu...
M Fuhr, C Stührk, F W M R Schwarze, M Schubert, H J Herrmann


A genomic sequencing approach to study wood decay and copper tolerance in the brown rot fungus, Antrodia radiculosa
2010 - IRG/WP 10-10720
We used Illumina paired-end short read sequencing (76 nt, 300 bp insert size) to produce a de novo assembly of the genome of Antrodia radiculosa, a copper-tolerant brown rot fungus that is capable of aggressive wood decay. Quality analysis of the base calls in the dataset (8.95 Gb) showed that the majority of the nucleotide sequence was of the highest quality with 5% in the lowest quality group. ...
J D Tang, T Sonstegard, S Burgess, S V Diehl


Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze


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