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Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl


Validity of above ground testing according to ENV 12037
2008 - IRG/WP 08-20379
The standard ENV 12037 for testing of treated and untreated wood (lap-joint) exposed above ground was issued in 1996 by The European Committee for Standardization. A draft for the development of the standard was presented by CEN-TC 38/WG 25/DOC N 076 in 2002. For a test to become valid the median rating of decay for untreated control replicates (Scots pine sapwood) must be equal or greater than ...
Ö Bergman, U Råberg, N Terziev


Molecular Methods: a Reliable Tool for the Identification of Wood Decay Fungi in Construction Timber
2008 - IRG/WP 08-20386
In the present study, we tested the practical value of several DNA-based methods to identify at the species level the most common wood-decaying fungi infecting buildings. We successfully extracted and amplified fungal DNA from pure cultures of twelve species of wood-inhabiting fungi, from oak and pine wood infected in laboratory with known strains, and from unknown field samples of wood collected ...
M Maître, M Kutnik, I Le Bayon, L Harvengt


Preliminary evaluation of efficacy of copper in combination with boron and NHA (N'-N-(1, 8-naphthalyl) hydroxylamine) against wood decay
2008 - IRG/WP 08-30452
In this study, efficacy of various combinations of copper sulfate with either boric acid or calcium precipitating agent, NHA-Na (N'-N-(1, 8-naphthalyl) hydroxylamine) was evaluated. Wood specimens were treated with either 1%, 0.5%, or 0.1% concentrations of copper sulfate, boric acid, NHA-Na, copper sulfate + boric acid, or copper sulfate + NHA-Na mixtures. Treated specimens were subjecte...
E Terzi, C Köse, S N Kartal


Investigation into the antifungal properties of herbal remedies for potential use in the wood preservation industry
2008 - IRG/WP 08-30462
The efficacy of herbal remedies as potential antifungal agents was evaluated against two sapstain and three decay fungi. Sapstain fungi included Sphaeropsis sapinea, and Leptographium procerum, and decay fungi were Oligoporus placenta, Coniophora puteana, and Schizophyllum commune. Out of six herbal remedies tested, three showed moderate to high antifungal activity. Caprylic acid had high antif...
D O’Callahan


Effect of amphiphilic antioxidant alkyl ammonium ascorbate on inhibition of fungal growth: Application to wood preservatives formulation
2008 - IRG/WP 08-30466
Amphiphilic surfactants were synthesized from ascorbic acid and different fatty amines and evaluated for formulation of wood preservation products. Characterization of physicochemical properties of the new compounds indicated classical behavior of cationic surfactants. Antioxidant properties, estimated using methyl linoleate oxidation inhibition method, were similar to that of ascorbic acid teste...
C Gérardin , T Koumbi Mounanga, P Gérardin


Performance of wood-based composites in a protected aboveground test in southern Japan
2008 - IRG/WP 08-40391
Five kinds of untreated wood-based composites (medium density fiberboard, hardwood plywood=HP, softwood plywood, particleboard and aspen oriented strand board, hereinafter abbreviated as MDF, HP, SP, PB and OSB, respectively) were exposed to subterranean termites and microbial (decay) attack under protected aboveground conditions in a southern part of Japan (Kagoshima Prefecture) for six years. An...
K Tsunoda


Feasibility study on three furfurylated non-durable tropical wood species evaluated for resistance to brown, white and soft rot fungi
2008 - IRG/WP 08-40395
Furfurylation can protect non-durable wood species against biological degradation, but the method used today cannot fully protect the heartwood of Scots pine due to insufficient penetration. In order to test alternative wood substrates for furfurylation, three Malaysian grown wood species (Kelempayan, Rubberwood and Sena) were furfurylated and subjected to soil block decay testing. Their performan...
T Mark Venås, A H H Wong


Preliminary investigation of biological resistance, water absorption and swelling of thermally compressed pine wood panels
2008 - IRG/WP 08-40396
Wood can be modified by compressive, thermal and chemical treatments. Compression of wood under thermal conditions is resulted in densification of wood. This study evaluated decay and termite resistance of thermally compressed pine wood panels at either 5 or 7 MPa and at either 120 or 150?C for one hour. The process caused increases in density and decreases in thickness of the panels; however, lab...
Ö Ünsal, S N Kartal, Z Candan, R Arango, C A Clausen, F Green III


The effects of zinc borate treatment of wood strands on some technological properties of oriented strandboard (OSB)
2008 - IRG/WP 08-40419
In this study, influences of zinc borate treatment on some technological properties of oriented strandboard (OSB), from aspen wood (Populus euroamericana cv.) were evaluated. Therefore, a total of nine panels with dimensions of 40 cm by 40 cm by 1.8 cm and with an average target density of 0.65 g/cm3 were manufactured under the laboratory conditions. Experimental panels were manufactured at two di...
A D Çavdar, H Kalaycioglu, G Nemli


Improvement of the Fungal Resistance of Japanese Cedar by the ThermoWood Process
2008 - IRG/WP 08-40422
The effect of the ThermoWood Process on sapwood of the Japanese cedar, Cryptomeria japonica, was investigated. Seven matched specimens were cut from a sapwood board of Japanese cedar and subjected to thermal treatments according to the ThermoWood Process. The decay resistance and chemical and physical properties of the treated specimens and untreated specimen were investigated. The decay test wa...
I Momohara, T Morita, S Shouho, A Yamaguchi


Field Tests of naturally Durable North American Wood Species
2008 - IRG/WP 08-10675
There has been little field test performance data published on North American naturally durable species in general, and no published data on second growth material in particular. Yellow cedar (Callitropsis nootkatensis), western red cedar (Thuja plicata), eastern white cedar (Thuja occidentalis), and three wood species reputed to be moderately durable were installed in ground-contact (stakes) an...
P E Laks, P I Morris, G M Larkin, J K Ingram


The Effects of Solar Radiation on the Fungal Colonization and Color of Weathered Wood
2008 - IRG/WP 08-10676
Solar radiation rapidly depolymerizes lignin at wood surfaces exposed outdoors producing a suite of low molecular weight aromatic compounds within weathered wood. Fungi colonizing weathered wood are able to metabolize these aromatic compounds, and many of them also contain high levels of melanin to protect themselves from the harmful effects of UV radiation. Both of these adaptations suggest that ...
V Hernandez, C Breuil, P D Evans


Wood Decay Research Using Molecular Procedures, What Can It Tell Us?
2008 - IRG/WP 08-10678
There are many unanswered questions in wood decay and protection research, and no single research technique will ever answer all of these questions. The process of wood decay is a very complex series of biochemical and chemical reactions that are heavily influenced by the hundreds of bacterial and fungal species found on environmental samples of wood. There are a variety of molecular techniques b...
S V Diehl, M. L Prewitt, Young-Min Kang, L Mangum, J D Tang


Aislamiento, identificación y evaluación enzimática de hongos de pudrición de madera de la Región de los Lagos
2008 - IRG/WP 08-10680
So far, systematic investigations have not been developed to determine the fungal diversity associated with wood in Chile. In addition, little is known about the mycoflora capabilities or their enzymatic processes in biotechnology although different research has hinted at the great potential of these microorganisms in various industrial processes. The aim of this work is to isolate, identify and e...
R Ortiz, J Navarrete, C Oviedo, R Blanchette


Measurement of wood decay by dynamic MOE in an accelerated soil contact test
2008 - IRG/WP 08-20390
Current laboratory and field testing of preservatives involves various techniques to determine the extent of fungal attack, including visual inspection, mass loss, and static bending and compression strength measurements. The objective of this study was to compare decay measurement by conventional compression strength versus dynamic MOE, employing small wood stakes in an accelerated laboratory so...
Gan Li, D D Nicholas, T P Schultz


Effect of different ASAs (alkenyl succinic anhydrides) on the treatment of biological protection of wood in use class 4
2008 - IRG/WP 08-40440
The alkenyl suscinic anhydrides (ASAs) used in this work are adducts resulting from the maleinization reaction of fatty acid alkyl esters. Various products with different alkyl groups were synthesized from rapeseed (Brassica napus) oil esters, rich in oleic acid. The liquids obtained showed a viscosity similar to that of vegetable oils and a brown color. When applied into wood, a thermal treatment...
C Vaca-Garcia, O Pignolet


Evaluation of the natural durability of reed (Phragmites australis) originating in the Fertö region (Hungary) against wood decay organisms as material for use in a sustainable motorway noise barrier
2009 - IRG/WP 09-10685
This work presents the research carried out to determine the natural durability of reed from the Fertö region of Hungary against wood decay organisms. The final objective of this study is to obtain sufficient relevant data in order that the reed may be used as a constituent element in a viable and sustainable motorway noise barrier, which has been developed within the European research project, k...
M T Troya, F Rubio, M J Prieto, D Lorenzo, J L Fernández-Cabo, R Schöftner


The involvement of hydroxyl-radical-producing glycoprotein from the white-rot basidiomycete Ceriporiopsis subvermispora in wood decay
2009 - IRG/WP 09-10688
White-rot decay can be divided into two subtypes. One type involves the simultaneous degradation of all wood components through the formation of erosion troughs with a progressive thinning of wood cell walls. This type of decay is consistent with a model in which several polymer-degrading enzymes act on the exposed surfaces of the wood cell walls, producing progressive erosion from the lumen to th...
H Tanaka, Y Inoue, T Morikawa, S Itakura, A Enoki


Discovering anti-fungal agents in New Zealand native plants for use in wood protection
2009 - IRG/WP 09-10692
Extracts from Hinau (Elaeocarpus dentatus) leaves were tested in the laboratory for antifungal activity. In addition, the anatomical structure of the leaf was also examined by a combination of light and scanning electron microscopy. Chemical characterisation of the extracts and investigation into potential use of its components for wood protection is underway. A combination of light microscopy (...
C Rickard, T Singh, A Singh, R Newman


Impact of decay and blue stain causing fungi on the structural integrity of wood
2009 - IRG/WP 09-10699
The potential influence of diverse decay patterns caused by different brown rot provoking basidiomycetes on the structural integrity of wood was investigated. Additionally the potential impact of blue stain on the structural integrity was examined. Therefore decayed Norway spruce (Picea abies Karst.) specimens representing a wide range of mass loss, caused by four different brown-rot fungi and one...
C Brischke, C Welzbacher, T Huckfeldt, F Schuh


The effect of different foundation systems against fungal flora in the crawl space of a new wooden Japanese house
2009 - IRG/WP 09-10700
In order to establish novel preventive measures against damage of wooden houses by decay fungi with less-use or no-use of chemicals, we periodically monitored the fungal flora in the crawl space of a new wooden Japanese house build with recyclable and low-environmental load materials at the Research Institute for Sustainable Humanosphere, Kyoto University. We employed either a layer of concrete or...
A Toyoumi, S Horisawa, T Yoshimura, S Doi,Y Imamura


Postia placenta gene expression of oxidative and carbohydrate metabolism related genes during growth in furfurylated wood
2009 - IRG/WP 09-10701
A range of studies the last decade have shown that modified wood can provide excellent protection against a range of wood deteriorating organisms, including decay fungi. However, we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward e.g. inhibition of action of specific enzymes, but they still need testing. An understandi...
G Alfredsen, C G Fossdal


Gene expression of selected decay enzymes produced during biodeterioration of three wood types
2009 - IRG/WP 09-10702
Comparative studies were conducted on the expression of several decay enzyme genes during the decay of pine, cedar, and ACQ treated pine over 10 months. Measurements of MOE, decay rating, and moisture control were monitored for physical properties. Identification of decay fungi and the detection of decay enzymes were carried out before gene expression levels were measured. The MOE of wood stakes d...
Young-Min Kang, L Prewitt, S Diehl


Degradation of wood veneers by Fenton’s reagents: effects of low molecular weight phenolic compounds on hydrogen peroxide decay and tensile strength loss
2009 - IRG/WP 09-20400
Pine wood (Pinus sylvestris) veneers strips were incubated in acetate buffer containing hydrogen peroxide and iron to mimic mechanisms of brown rot decay and assess the degradation of cellulose through analysis of the tensile properties of the decayed wood. The tensile properties of thin wood strips treated with Fenton system reagents or precursors were determined and correlated to weight loss as ...
Yanjun Xie, R Well, Zefang Xiao, B Goodell, J Jellison, H Militz, C Mai


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