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Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Breakdown of cellulose derivatives by cellulolytic enzymes. I. Action of fungal ß-glucosidases toward substituted PNP ß-D-glucopyranosides
1998 - IRG/WP 98-10281
The action of fungal ß-glucosidases on the methyl derivatives of p-nitrophenyl (PNP) ß-D-glucopyranoside, which were regioselectively substituted at 0-2, 0-3, 0-4 and 0-6 positions was studied. Several ß-glucosidases from brown-rot, white-rot, and soft-rot fungi and almond were used for the study. These ß-glucosidases did not act on the 2, 3, and 4-0-methyl derivatives, while the 6-0-methyl on...
T Nishimura, I Momohara, M Ishihara


Improved preservative penetration of spruce after pre-treatment with selected fungi. I. Fungal pre-treatment of pole sections
1998 - IRG/WP 98-40117
European spruce logs were pre-treated with either Phanaerochete chrysosporium, Dichomitus squalens, Trichoderma viride and Trichoderma aureoviride for incubation periods of between 1-4 weeks prior to pressure treatment with creosote in a commercial treatment plant and analysed for strength and creosote uptake (see part II Tucker et al.). A method to pre-treat logs was optimised. The pole sections ...
B Rosner, K Messner, E J B Tucker, A Bruce


The suitability of isothiazolone microemulsions as long term wood preservatives
1997 - IRG/WP 97-30150
Microemulsion formulations of 4,5 dichloro-2-n-octyl-4-isothiazolin-3-one were investigated to determine the leaching potential of these formulation types as well as their efficacy against decay fungi. These patented low leaching formulations exhibited good anti fungal efficacy in standard tests combined with minimal leaching of the active ingredient from the wood. The data suggests that the excel...
B M Hegarty, Bing Yu, L E Leightley


Artificial weathering exposure as an alternative for standard ageing according to EN 84 (leaching) and EN 73 (evaporation)
2002 - IRG/WP 02-20254
In order to verify potential weaknesses of wood preservatives fungal tests are carried out after ageing of wood preservative treated test blocks. The European standard EN 599 as a framework for efficacy assessment of wood preservatives includes the use of two ageing methods prior to fungal testing, namely a leaching method (EN 84) and an evaporation method (EN 73). The European research project "F...
J Van Acker, M Grinda, D J Dickinson


Characteristics and quantity of impregnated wood waste in Germany
1995 - IRG/WP 95-50041
The disposal of wood waste in Germany is one of the main problems not only for the wood preservation industry but also for all concerned with wood waste. Data on characteristics and quantity of wood waste are still needed. Based on criterions given in a previous paper (IRG/WP 93-50006), several assortments have been characterized with regard to their hazardous potential and their logistical aspect...
A Voss, H Willeitner


Growth of the copper tolerant brown rot fungus Antrodia vaillantii on different substrates
1995 - IRG/WP 95-10121
In recent years the copper tolerant brown rot fungus Antrodia vaillantii caused severe damages on impregnated wood in ground contact. The pattern of decay gave the impression that impregnated wood was even more severely attacked than unimpregnated. To investigate this question more closely laboratory tests were carried out. In a "choice test" Antrodia vaillantii grew preferably towards CC-impregna...
H Leithoff, I Stephan, M-T Lenz, R-D Peek


Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti


Site characteristics impacting historic waterlogged wood: A review
2000 - IRG/WP 00-10344
Survival of waterlogged wood from hundreds and in rare cases millions of years presents scientists with a unique opportunity to examine wood specimens which, due to select properties of the wood itself and/or the depostional environment, have not been completely degraded. Although degradation patterns of various types of microbial wood decay have been studied in detail, the site parameters of the ...
B A Jordan, E L Schmidt


Effect of origin and orientation of Radiata pine substrates on the development of fungal degrade
2000 - IRG/WP 00-30221
The objective of this study was to determine the influence of different radiata pine substrates on colonisation by sapstain fungi. Freshly-cut branch wood, stem wood and sawn lumber were used to prepare test samples with the largest wood surface area showing a transverse, radial or tangential face. Test samples were evaluated against sapstain fungi using a rapid laboratory antisapstain screening m...
B Kreber, D R Eden, C M Chittenden, B Carpenter, J G Van der Waals


Mould growth at lumber surfaces of pine after kiln and air drying
1994 - IRG/WP 94-40033
Distribution of water soluble substances in green wood and later redistribution during drying is of importance for the colonisation of wood by microorganisms. According to literature the availability of nitrogenous materials is probably a major limiting factor to the microbial colonisation of wood. King et al. (1974) and Oxley et al. (1976) have shown correlation between surface nutrient concentra...
N Terziev, J Bjurman, J B Boutelje


Hyphal tunnelling of belian (Eusideroxylon zwageri) wood cell walls
1996 - IRG/WP 96-10152
Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods. Visual inspections at the groundline of belian transmission poles from Sarawak, Malaysia, showed only surface decay of wood after 20 years in service. The cause of decay in belian was investigated by light and transmission electron microscopy. Light microscopy of transverse sections of surface tissue...
A P Singh, A H H Wong


Variations in the virulence of test strains of Coniophora puteana (Schum ex Fr) Karst
1982 - IRG/WP 2185
In laboratory experiments using petri-dishes and small wood blocks (30 x 10 x 5 mm³) 6 different isolates of Coniophora puteana strain BAM 15 were compared with strain FPRL 11E for growth rate and decay capability. Only the recently received strain from EMPA was suitably active giving 24% weight loss after 6 weeks compared with 38.1% for 11E. Collation of data from standard test records revealed ...
A F Bravery, J K Carey, W Worley


Characteristics of the pigments produced by sap-staining fungi
1997 - IRG/WP 97-10223
The present study was conducted to characterize the pigments produced by sap-staining fungi, Aureobasidium pullulans (de Bary) Arnaud and Ceratocystis piceae (Münch) H. and P. Sydow. The pigments isolated from tested fungi were identified as melanins by spectroscopic methods (UV and infrared spectra). The UV and the infrared spectra of the fungal pigments were similar to those of the synthetic me...
M Mori, M Takahashi


Characterization of Poria indoor brown-rot fungi
1995 - IRG/WP 95-10094
The heterogeneous group of "Poria" fungi causing brown rot in buildings and also of wood in ground contact comprises Antrodia vaillantii, Antrodia serialis, Antrodia sinuosa, Antrodia xantha and Tyromyces placenta. These fungi have similar morphological appearance and biology. Their nomenclature has a confusing history and is still not uniform. As a consequence, misinterpretations may occur. SDS p...
O Schmidt


Effect of crude tall oil, linseed oil and rapeseed oil on the growth of the decay fungi
2002 - IRG/WP 02-30299
The influence of crude tall oil, linseed oil and rapeseed oil on the growth of Coniophora puteana, Poria placenta and Coriolus versicolor was studied. The selected test oils were observed to have different effects on the the fungal growth. Crude tall oil inhibited the radial growth of all fungi. Rapeseed oil either accelerated or inhibited the growth of fungi depending on the type of fungus involv...
L Paajanen, A-C Ritschkoff


Gaseous treatment of timber with allyl isothiocyanate. Fungicidal and insecticidal effects
1996 - IRG/WP 96-30108
Gaseous treatment with allyl isothiocyanate (AIT) was tested for its effects on the growth of microorganisms on the wood substrate and the mortality of subterranean termites and powder-post beetles. Toxic limits of AIT were determined as concentrations in the air when an AIT-treated filter paper was placed in a sealed container with fungus-inoculated wood specimens. Those were <3.8 ppm for Aure...
K Tsunoda, T Yoshimura


Effects of air-seasoning on fungal colonization and wood strength of Douglas fir poles
1987 - IRG/WP 1315
Air seasoning economically reduces the moisture content of Douglas fir poles before pressure treatment with preservatives. Advanced decay in poles in service has resulted when decay fungi (Basidiomycetes) colonized poles during air-seasoning and survived the treatment process. These problems have led to recommendations to severely limit this practice. To determine the role of these fungi in peeled...
J J Morrell, M E Corden, R D Graham, B L Kropp, P Przybylowicz, S M Smith, C A Sexton


Colonisation of painted wood by Aureobasidium pullulans - Analysis of features and consequences for failure in service
1996 - IRG/WP 96-10144
Wooden blocks of spruce were painted with different paint formulations. Water- and solvent-borne model and commercial paints were used. The painted wooden blocks were inoculated with a spore suspension of Aureobasidium pullulans (de Bary) Berkg. and placed in an environment of high humidity for 14 months. Different colonisation and growth patterns were observed on the different paint surfaces. The...
S L Bardage


The influence of staining fungi on the decay resistance of wood treated with alkylammonium compounds
1984 - IRG/WP 3308
Although found to be very effective in laboratory tests, alkylammonium compounds (AAC&apos;s) have failed to perform as well in field stake tests. Examination of leachability showed that this was not the cause. The present study investigated the possibility that staining fungi, (which have been observed to rapidly infect the field stakes), degrade the AAC wood preservative. Soil-blocks were tr...
J N R Ruddick


Comparison of the in-ground performance of pigment emulsified creosote (PEC) and high temperature creosote (HTC)
2000 - IRG/WP 00-30217
A long-term field trial was conducted in Australia to compare the in-ground performance of two oil-borne preservative formulations, conventional high temperature creosote (HTC) and a modified creosote formulation, pigment emulsified creosote (PEC). Three retentions (50, 100 and 200 kg/m³) were targeted for each formulation. An additional retention of PEC formulation (308 kg/m³), which contained ...
J W Creffield, H Greaves, N Chew, N K Nguyen


The effect of added nutrients on growth rate and decay capacity of Serpula lacrymans
1990 - IRG/WP 1427
At the previous meeting a new technique was presented that enables both fungal growth rate and wood decay rate to be measured using the same timber specimen. The technique (IRG/WP/1384) has previously been carried out with 1% malt as the sole nutrient within the small jar that provides the inoculum for this method. Results presented here relate to an additional level of 5% malt, with or without a ...
J D Thornton, A McConalogue


Fungal detoxification of organotin biocides
1985 - IRG/WP 1258
The ability of a range of wood decaying fungi to inactivate bis(tri-n-butyltin) oxide (TnBTO) in the extracellular growth medium, in stationary liquid culture was determined. A distinction between the ability to tolerate the fungicide and to inactivate it was made: the white-rot organism Coriolus versicolor being the most efficient inactivator. In an attempt to determine the extracellular agents r...
P S Belford, D J Dickinson


Possible durability transfer from durable to non durable wood species. The study case of teak wood
2001 - IRG/WP 01-10392
Teakwood is well known for its excellent natural durability, mostly due to its high proportion of extracts. Amongst these extracts, quinones, and more precisely some naphtoquinones (such as lapachol) and anthraquinones (such as tectoquinone) appear to play a crucial role in the resistance to wood decay organisms. At a laboratory scale, sawdust from malaysian teak heartwood has been extracted under...
M-F Thévenon, C Roussel, J-P Haluk


Comparison of the anti-fungal efficacy of Cobra with drill perforation on oscillating pressure treated spruce transmission poles -Laboratory test
1988 - IRG/WP 3469
A laboratory method for measuring the depth of penetration of the fungicidal concentrations effective against basidiomycetes (brown rot) was developed. The tests on CCB oscillating pressure treated spruce transmission poles have shown that the depth of salt penetration can be increased by about 10 mm by a drill perforation before the impregnation. An even better penetration is reached, however, if...
E Graf, B Zgraggen


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