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Effectiveness of Copper Indicators in Treated Wood Exposed to Copper Tolerant Fungi
2014 - IRG/WP 14-20554
Wood treated with a copper based wood preservative will typically turn a green color. While the depth of copper penetration can be readily discerned from the green color of the copper it is standard practice in research and commercial treating plants to make use of a color reagent such as Chrome Azurol S, Rubeanic acid or PAN indicator to reveal the penetration more clearly. When copper treat...
L Jin, K Brown, A Zahora, K Archer


Decay resistance of wood-plastic composites reinforced with extracted or delignified wood flour
2014 - IRG/WP 14-40655
The moisture and decay resistance of wood-plastic composites (WPCs) reinforced with extracted or delignified wood flour (WF) was investigated. Three different extractions were preformed: toluene/ethanol (TE), acetone/water (AW), and hot water (HW). Delignification (DL) was performed using a sodium chlorite/acetic acid solution. All WPCs specimens were made with 50% by weight HDPE and WF, first com...
R E Ibach, Yao Chen, N M Stark, M A Tshabalala, Yongming Fan, Jianmin Gao


Development of new wood treatments combining boron impregnation and thermo modification - Effect on decay and termites durability
2014 - IRG/WP 14-40666
Environmental pressures appeared in most European countries during the last decade has lead to the development of more environmentally acceptable preservation methods. In this context, wood heat treatment has been one of the most investigated alternative methods during the last years. Treated at temperatures ranging between 180 and 220°C, wood components undergo more or less important chemical mo...
S Salman, A Pétrissans, M-F Thévenon, S Dumarçay, B Pollier, P Gérardin


Possible targets of wood modification in brown rot degradation
2014 - IRG/WP 14-40676
Wood modification protects wood from fungal degradation in a non-toxic manner. However, the mechanisms behind the decay resistance in modified wood are currently unknown. The aim of this study was to discuss the i) colonisation, ii) nutrient recognition, iii) transcription, iv) depolymerisation and v) hydrolysis steps in the brown rot degradation progress and explore whether they are inhibited by ...
R Ringman, A Pilgård, G Alfredsen, B Goodell, K Richter


Interpretation of moisture monitoring data - Relevance of moisture content thresholds for fungal decay
2015 - IRG/WP 15-10839
It is widely accepted that besides temperature wood moisture content (MC) is the key factor when it comes to durability of wooden structures exposed to weathering. Many approaches to predict the service life of wooden structures have therefore been based on using wood MC as one major input-variable to develop dose-response relationships between material climate and decay response. However, to impr...
L Meyer, C Brischke


Glutathione transferases and wood extracts
2015 - IRG/WP 15-10845
Wood rotting fungi have developed an efficient detoxification system allowing them to be well adapted to their way of life, these fungi generating and encountering potential toxic compounds during wood degradation. Belonging to this detoxification network, glutathione transferases are particularly extended in these fungi and their presence could reflect the chemical environments encountered by the...
A Deroy, F Saiag, Z Kebbi-Benkeder, N Touahri, M Morel-Rouhier, F Colin, S Dumarcay, P Gérardin,


The copper-transporting ATPase pump and its potential role in copper-tolerance
2016 - IRG/WP 16-10859
Copper-tolerant brown-rot decay fungi exploit intricate mechanisms to neutralize the efficacy of copper-containing preservative formulations. The production and accumulation of oxalate is the most widely recognized theory regarding the mechanism of copper-tolerance in these fungi. The role of oxalate, however, may be only one part of a series of necessary components required for this complex mecha...
K M Ohno, C A Clausen, F Green III, G Stanosz


Production of reactive oxygen species in the presence of oxidized iron and oxalate under conditions mimicking brown-rot fungal degradation of wood
2016 - IRG/WP 16-10861
The biochemical role of oxalate in the fungal solubilization of iron is well known, but additional information is needed on the role that oxalate plays in the brown-rot decay of lignocellulose. In this study iron sequestration from iron and iron oxide-hydroxides was assessed with regard to the function of a chelator-mediated Fenton (CMF) reaction and the generation of reactive oxygen species (ROS)...
Yuan Zhu, Liangpeng Zhuang, B Goodell, Jinzhen Cao, J Mahaney


High-frequency monitoring of mass loss due to brown rot degradation of modified wood
2016 - IRG/WP 16-10862
Fungi growing in liquid culture undergoes three separate phases in which they i) adapt to the new environment, ii) grow unrestrictedly and exponentially, and iii) are inhibited to increase in number/mass due to lack of nutrients etc. Filamentous fungi have been shown to exhibit similar growth phases in a solid food substrate and have been modelled to grow in this way also in solid wood. In modifie...
R Ringman, A Pilgård, K Richter


Fungicidal Effect of Tobacco Stalks (Nicotiana tabacum L.) Against Brown Rot Fungi “Coniophora puteana”
2016 - IRG/WP 16-10865
In this study it was investigated that the fungicidal effect of tobacco plant (Nicotiana tabacum L.), which is grown in many countries of the world, against brown rot fungi “Coniophora puteana”. The protective effect of this plant on Scotch pine (Pinus sylvestris L.) wood was considered by means of fungi decay test (EN 113). The wood samples have been treated by five different extracts; at 3%,...
Ü C Yıldız, S Yıldız, A Yılmaz, S Durmaz


Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
Prevention of wood decay by fungi of the phylum Basidiomycota is an important issue of wood protection. The most destructive fungus in buildings in Central Europe is the dry rot fungus Serpula lacrymans, which needs more extensive restauration measures than all other wood decay fungi. Its close relative S. himantioides occurs more frequently in outdoor environment. The assessment and remediation o...
K Jacobs, N Rangno


Resistance of heat treated Ash wood under steam pressure: rot fungi, soil micro-organisms and termites
2016 - IRG/WP 16-40724
Thermal modification processes have been developed to increase the biological durability and dimensional stability of wood. The aim of this paper was to study the influence of ThermoWood® treatment intensity on wood decay resistance improvement against soil-inhabiting micro-organisms, brown/white rots and termite’s exposures. All of the tests were carried out in the laboratory with two differen...
K Candelier, S Hannouz, M-F Thévenon, D Guibal, P Gérardin, M Pétrissans, R Collet


Superhydrophobic treatment of Norway spruce wood for improvement of its resistance against brown rot and moulds
2016 - IRG/WP 16-40734
Water repellence of wood can be increased and its some other properties influenced by introduction of hydrophobic chemicals into and on cell walls without affecting the wood’s bulk density due to lumen filling. Herein, a simple dipping method to insert octadecyltrichlorosilane (OTS) into cell walls and to deposit the self-assembled hydrophobic monolayers (SAMs) of OTS is reported. It was found o...
A Kumar, P Ryparová, P Hajek, B Kričej, M Pavlič, A S Škapin, M Šernek, J Tywoniak, J Žigon, M Petrič


Role of cell wall specific moisture content on the brown-rot fungal attack on wood
2016 - IRG/WP 16-40736
Wood is a hydroscopic resource because the cell wall polymers contain hydroxyl and other oxygen-containing groups that attract moisture through hydrogen bonding. Moisture content varies with changing moisture content in its environment. The moisture content in wood is responsible for many of the performance properties we observe. The strength properties of wood are dependent on the moisture con...
R M Rowell


Prevention of fungal damage of oil and date palm wood by organic acids
2017 - IRG/WP 17-10877
Felled palm trunks are susceptible to fungi as long as their moisture content is above fibre saturation. During this period, palm wood has to be protected against mould and rot fungi. Environmental-friendly organic acids are suitable. Small samples of oil palm (Elaeis guineensis) and date palm (Phoenix dactylifera) wood were treated with 1 to 10% solutions of acetic acid and propionic acid, respec...
M Bahmani, O Schmidt


The First Description of Soft-rot Cavity in Waterlogged Archaeological Woods by a Japanese Wood Anatomist Prof. F. Onaka in 1935
2017 - IRG/WP 17-10885
The term soft rot, which was caused by Ascomycetes and Deuteromycetes, was first used by J G Savory in 1954. Soft rot decay can be characterized by cavities within secondary cell walls align along the cellulose microfibrils. Our recent literature search revealed, however, that Prof. F. Onaka in Kyoto University described the soft rot cavities in the waterlogged archaeological woods in detail from ...
Yoon Soo Kim, K Yamamoto


Untreated and copper-treated wood soaked in sodium oxalate: Effects of decay by copper-tolerant and copper-sensitive fungi
2017 - IRG/WP 17-10888
Copper is widely used as the primary component in wood protectants because it demonstrates a broad range of biocidal properties. However, a key concern with using copper in wood preservative formulations is the possibility for brown-rot basidiomycetes to resist the toxic effect. Many brown-rot basidiomycetes have evolved mechanisms, like the production and accumulation of oxalate, which helps thes...
K M Ohno, G T Kirker, A B Bishell, C A Clausen


Thermal stability of a wood protective biofinish and the influence of flame retardants on Aureobasidium cells
2017 - IRG/WP 17-30716
In general there is an increasing need for sustainable resources, including sustainable building materials. Wood is one of these sustainable resources and is more used as an outdoor building material. Since unprotected wood will usually be degraded during outdoor exposure, many different protection systems are available on the market. Protection systems containing biocides however might create obs...
S Rensink, E A M Klein Rot, M F Sailer


Agar block durability test of plywood made of neem (Azadiracta indica) leaves treated veneer using two white rot fungi
2017 - IRG/WP 17-40782
This study has been undertaken to investigate the durability of plywood made from non-durable wood (simul, Bombax ceiba) veneer treated by neem (Azadiracta indica, A. Juss.) leaves. The veneers were treated with cold and hot water solution of neem leaves of different concentrations (10%, 5%, 2.5%) and treatment durations (1, 2, 3 days) for cold water and 20, 40 and 60 minutes for hot water. The pl...
K Akhter, M A Hashem, S Akhter


Biodegradation of creosote by white rot fungus Phanerochaete chrysosporium and a strains of Pseudomonas
2017 - IRG/WP 17-50329
The aim of this study was to determine degradation effectiveness of polycyclic aromatic hydrocarbons (PAH) contained in creosote by white rot fungus and bacteria. The following two species of bacteria Pseudomonas putida and Pseudomonas sp. as well as Phanerochaete chrysosporium fungus characterised by the ability to decompose aromatic compounds were selected for experiments. Bacterial strains were...
J Zabielska-Matejuk, A Stangierska, A Kropacz, E Kaczorek


Effects of exposure time on detection of the behavior of white-rot fungi on Oriental beech wood properties
2017 - IRG/WP 17-10902
The comparison of fungal degradation of Orientalis beechwood by two white-rot fungi was evaluated in this study. Biological, mechanical, and chemical assessments of beech wood were carried out at 30 day interval for 120 days. The results demonstrated that both fungi have greater effects on beech wood during each exposure time. However, Trametes versicolor degraded beech wood more effectively than ...
E Bari, D D Nicholas, K M Ohno


Interactions between wood polyphenols and detoxification enzymes of the white rot Trametes versicolor
2018 - IRG/WP 18-10906
Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases (GSTs) is especially expanded in lignolytic fungi, little is known about their physiological target molecules. In this study, by combining thermal shift assay and affinity crystallography we highlighted inter...
M Schwartz, T Perrot, E Aubert, S Dumarçay, F Favier, P Gérardin, M Morel-Rouhier, G Mulliert, F Saiag, C Didierjean, E Gelhaye


Enhancing Our Understanding of Brown Rot Mechanisms through Catalytic Pretreatment and Cellulase Cocktail
2018 - IRG/WP 18-10909
A catalytic mechanism, described as the “chelator-mediated Fenton” (CMF) mechanism, is proposed to mimic the non-enzymatic action of brown rot fungi. A CMF treatment was used together with an enzymatic cocktail to study how wood was deconstructed and solubilized. This was done in-part to determine if the treatment mimicked the action of brown rot fungi, but also to explore improved treatment p...
S Tabor, L Orjuela, D Contreras, G Alfredsen, J Jellison, S Renneckar, B Goodell


Preliminary Investigation into the Natural Decay Resistance of Nigerian Grown Hevea brasiliensis and Mitragyna ciliata wood to Phanerochaete chrysosporium White-Rot Fungus
2018 - IRG/WP 18-10919
Wood is an important and versatile structural building material that finds applications in numerous uses. However, wood is also a biological material vulnerable to degradation by microbial activities; this is especially true in the tropics. Following the scarcity of highly durable species from our natural forests and the introduction of so many lesser used/durable wood species into the booming tim...
J M Owoyemi, U O Emmanuel


Mechanism of nano metal fluorides against fungi
2018 - IRG/WP 18-30730
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredient in wood preservatives such as copper and boron. Wood specimens treated with fluoride sols of MgF2 and CaF2 have been tested against brown-rot fungi (Coniophora puteana and Rhodonia placenta) in accordance with EN 113 and against termites (...
S M Usmani, K Klutzny, I Stephan, T Hübert, E Kemnitz


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