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Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


In situ testing the influence of melamine resins on the enzymatic activity of basidiomycetes
1999 - IRG/WP 99-30194
Waterbased methanol etherificated melamine formaldehyde resins have the potential to increase the resistance of impregnated wood against wood destroying fungi. The mechanism of the increased wood durability is not clear yet. In the present paper the possible interference of melamine resins with wood degrading enzymes of Coniophora puteana and Trametes versicolor was investigated as a possible cont...
D Lukowsky, F Büschelberger, O Schmidt


Soft rot tests with soils of different origins
1984 - IRG/WP 2226
In the BAM a comparative soft rot test with 9 different types of soil (one soil each from Canada, New Zealand and Switzerland and 6 soils from laboratories in Germany) were carried out. The pH values of the different soils ranged from 3.2 to 7.1, and the amounts of organic matter were between 4 and 44% (w/w). The test specimens - Pine sapwood blocks treated with different concentrations of a CCA p...
M Gersonde, W Kerner


The true dry rot fungus (Serpula lacrymans) as a wound parasite of living Norway spruce (Picea abies) in the Czech Republic
2001 - IRG/WP 01-10389
Some late 19th century Central European mycologist reported finds of the true dry rot fungus (Serpula lacrymans) from nature. They even suggested that it could be a parasite of living trees. However examination of herbarium material has shown that the species they found was thin-fleshed dry rot (Serpula himantioides) rather than Serpula lacrymans. In 1992 the Czech mycologist Kotlaba reported find...
J Bech-Andersen, S A Elborne, J Andreasson, J Ch Sterler


Biological degradation resistance of wood acetylated with thioacetic acid
1983 - IRG/WP 3223
Chemically, modification of wood is being considered as an alternative to conventional preservation by toxic chemicals. Acetylated wood has been reported to be quite resistant to most biodegrading organisms at weight percent gains (WPG) around 15-19. The conventional acetylation techniques with acethic anhyrdride result in generation of acetic acid. However, acetylation with thioacetic acid overco...
S Kumar, S C Agarwal


An unusual soft-rot decay pattern caused by the Ascomycete Hypoxylon mediterraneum (de Not.) J Miller
1984 - IRG/WP 1222
A distinct pattern of soft-rot decay has been observed for the fungus Hypoxylan mediterraneum (de Not.) J. Miller. This fungus also produced decay patterns typical of brown and white rot decay. The production of characteristic cavities by Hypoxylan mediterraneum was prolific in the hardwoods Eucalyptus maculata and Eucalyptus regnans, but infrequent in the softwoods, Pinus elliottii and Pinus radi...
D M Francis, L E Leightley


Soft rot test of copper/chrome/arsenic treated heartwood of three Malaysian timbers by the vermiculite-burial method. (+ correction document of 25 July 1990)
1990 - IRG/WP 2354
Heartwood of copper-chrome-arsenic (CCA) treated kempas (Koompassia malaccensis), tualang (Koompassia excelsa) and keruing (Dipterocarpus spec.) was found to be susceptible to soft rot in recent pole surveys. Standardized heartwood blocks were impregnated with 0 to 6.3% (w/v) CCA and challenged to decay for twelve weeks by a mixed inocula of Chaetomium globosum, Glenospora graphii, Humicula grisea...
R-D Peek, A H H Wong


Chemical and biological investigations of double-vacuum treated windows after 7½ years in service
1985 - IRG/WP 3339
Earlier investigations of double-vacuum treated windows after five years in service have shown that tributyltin oxide (TBTO) degrades to di- and monobutyltin compounds and that the resistance of the wood against decay decreases. An investigation 2.5 years later of the same windows shows that the degradation of TBTO proceeds fast. After 7.5 years in service only 15-35% of the remaining organotin co...
M-L Edlund, J Jermer, B Henningsson, W Hintze


The effect of copper/chrome/arsenic (CCA) treated timber on soil fungi
1981 - IRG/WP 1131
The aim of this investigation was to study the effect of CCA treated wood on the microflora of soil with particular reference to copper tolerant soft rot fungi. The IRG collaborative field experiment was designed to monitor the performance of a range of preservative treated hardwoods with particular reference to soft rot in CCA treated timber (Dickinson 1976) and site 33 of this experiment was use...
R J Murphy, D J Dickinson


Objectives of future collaborative soft rot tests
1972 - IRG/WP 209
Progress has been made towards acceptance of a defined laboratory test method for determining the toxicity of preservatives against soft rot fungi (Documents IRG/WP/201 and IRG/WP/208) and, at the 1971 meeting in Brussels, the framework of collaboration for the next phase of testing was discussed. Time available at Brussels did not permit restatement of objectives, consideration of the stages by w...
J G Savory


Evaluation of a laboratory soft rot test on basis of weight loss and residual strength
1989 - IRG/WP 2336
Based on the principles of different previously reported experimental procedures dealing with laboratory soft rot testing, a study was conducted on treated poplar and beech. Preservatives used were CCA, CCF, AAC, Azaconazole and TCMTB. Treated samples were exposed for three months in a vermiculite substrate to Chaetomium globosum. Evaluation was based on absolute mass loss, percentage weight loss ...
W Verbeke, J Van Acker, M Stevens


The development of soft rot decay in bamboo fibres
1992 - IRG/WP 92-1572
The development of decay by soft rot fungus Chaetomium globosum was investigated in young and mature culms of bamboo Phyllostachys virideglaucescens. Soft rot decay was influenced both quantitatively and qualitatively by the differing levels of lignification in the material. Weight loss was significantly greater in the younger culm compared with the older, more heavily lignified culm. The distribu...
O Sulaiman, R J Murphy


Potential toxicants for controlling soft rot in preservative treated hardwoods. Part 2: Laboratory screening tests using sawdust
1977 - IRG/WP 2101
A technique is described for the rapid screening of toxicants against microorganisms, represented by the brown rot, Gloeophyllum trabeum, and a natural inoculum from soft rotted wood. Sawdust, in this case from sapwood of Eucalyptus maculata, is pretreated with the candidate formulation and moistened before inoculation. Visual scoring of growth can be used to discriminate suitable candidates as ea...
J D Thornton


Serpula lacrymans the dry rot fungus. Revue on previous papers
1989 - IRG/WP 1393
It is found that the Dry rot fungus Serpula lacrymans grows in houses only because of its need for basic materials to neutralize the oxalic acid production or heavy metals which celate the oxalic acid. The average distance from the mycelium to the basic materials is found in average to be 14.2 cm with a variation from 0-100 cm. In contrast to Serpula lacrymans the Coniophora puteana and the Rigido...
J Bech-Andersen


Production of monoclonal antibodies to Serpula lacrymans and their application in immunodetection systems
1993 - IRG/WP 93-10004
Monoclonal antibodies were produced against both whole cell mycelial extracts and exo-antigen extracts of the causative organism of dry rot, Serpula lacrymans. The antibodies were tested for cross-reactivity against fifteen strains of Serpula lacrymans and nine other fungal species. These species represented either other wood decay fungi or non-decay fungi commonly co-isolated with Serpula lacryma...
H Glancy, J W Palfreyman


Microscopic FT-IR depth profiling study of photo-induced degradation in wood
2001 - IRG/WP 01-20229
FT-IR microspectroscopy was used to monitor the changes in the chemical composition at various depths from the photo-irradiated surface of sugi (Cryptomeria japonica D. Don) sapwood. The radially cut wood face was exposed to artificial solar radiation from a xenon light source at 375 Wm-2 (in a 300 - 700 nm spectral range) for up to 600 h. The IR depth profiling spectra showed that the artificial ...
Y Kataoka, M Kiguchi.


Bacterial degradation of wood cell wall: A review of degradation patterns
1990 - IRG/WP 1460
Information from bacterial degradation studies of 60's and 70's was reviewed by Nilsson in 1982. The application of electron microscopy to this area in recent years has provided much useful information and has eliminated earlier scepticism among workers about the ability of bacteria to degrade lignified wood cell walls. Studies using transmission electron microscopy together with...
A P Singh, J A Butcher


Studies in an accelerated soil bed facility on the decay susceptibility of U.K. grown spruce and pine poles treated with copper/chrome/arsenic (CCA) by pressurised sap-displacement. Part 1: Setting up of soil beds and initial soft rot results
1990 - IRG/WP 2344
The paper describes the methodology used in the construction and early operation of an accelerated soil bed facility used to examine the decay susceptibility of U.K. grown Scots and Corsican pine and Sitka and Norway spruce treated with C.C.A. by high pressure sap-displacememt. The design and control of the facility as well as the preparation, soil exposure and soft rot decay analysis of quarter p...
A Bruce, S D Hainey, G M Smith, B King, P D Evans


Soft-rot testing. Memo to CEN
1983 - IRG/WP 2206 A
1. Development of a single test procedure to assess performance of preservatives against soft rot fungi is an ideal that cannot be realised at present, if results are to be both reproducible between laboratories and pertinent to the practical requirements of individual countries. 2. Consensus opinion amongst members of Sub Group I - Soft rot tests, of Working Group II - Fundamentals of Testing, of the IRG, is that the immediate needs of CEN would be best answered by: (a) a code of recommended practice, (b) a multiplicity of test procedures, and (c) a degree of flexibility in test procedures. 3. The code of recommended practice should recognise the need for: (a) a pure culture, synthetic medium (sand or vermiculite + nutrients), single wood species test to be adopted by all laboratories as a common reference point of testing, (b) mixed inoculum and/or unsterile soil laboratory or fungus cellar tests which account for local variations in the fungal flora, (c) incorporation of a range of wood species where applicable (i.e. where mixed hardwoods comprise the main resource), (d) provision of local internal standards of known performance under field conditions, (e) incorporation of leaching procedures other than standard methods with deionized water (e.g. mild acid, milk alkali, ionic solutions of various salts) where applicable to local exposure conditions, (f) precise definition of the criteria used for establishing toxic thresholds (mass loss, strength loss, microscopic examination). 4. Initial screening tests should remain the province of the individual research worker. 5. The code of recommended practice should have the sole purpose of establishing provisional retention levels for subsequent confirmation by field trial. 6. IRG should list and approve test procedures considered adequate to establish toxic thresholds for preservatives against soft rot fungi. 7. It should be clearly understood that when using a series of test techniques, it is inevitable that a range of toxic thresholds will be obtained for the same preservative. 8. Guidelines should be drawn up for interpretation of test data for subsequent transformation to provisional retention levels. 9. Adoption of the broad principles outlines above is recognition of the dynamic nature of toxic thresholds. The additional information obtained by a more flexible, and complex, approach to testing may make interpretation more difficult but should provide data from which response of preservatives to varying biological and environmental hazards can be more realistically assessed. 10. It is recommended that CEN adopt, in principle, the basic philosophy of testing outlined above and move towards formulation of a Working Document in collaboration with IRG
J A Butcher, D J Dickinson


List of fungi in soft-rot tests
1975 - IRG/WP 105
This list gives information on the terrestrial and marine species and strains of fungi tested for soft-rot ability in pure culture with data on their decay activity in untreated wood. An index of the timber species tested is added. The compilation is based on 1) the list of soft-rot fungi of the former OECD Group (Doc.No. 27/DAS/CSI/M/559), Oct. 1968, prepared by R. Rösch and W. Liese, 2) informa...
G Seehann, W Liese, B Kess


The degradation of wood surfaces by dilute acids
1985 - IRG/WP 3326
Thin radial/longitudinal sections(~100 µ) of Corsican Pine (Pinus nigra) and Lime (Tilia vulgaris), were exposed to Sulphuric, Sulphurous, nitric, acetic, and formic acid at 40°C in the pH range 2-6. After about 3 months exposure to Sulphuric, nitric, acetic and formic acid at pH 2.0 Pine lost some 20-25% of its tensile strength. Losses in tensile strength due to sulphurous acid were greater bei...
P D Evans, W B Banks


Free radical process controlled by manganese peroxidase and lipid-related metabolites produced by Ceriporiopsis subvermispora
2001 - IRG/WP 01-10412
Ceriporiopsis subvermispora, a specific lignin-degrading fungus produced free unsaturated fatty acids (USFAs) including 9,12-octadecaienoic asid, together with saturated fatty acids (SFAs) at an incipient stage of cultivation on wood meal cultures. In prolonged cultivation period after two weeks, the amount of intact fatty acids decreased with increasing in organic hydroperoxide and TBARS producti...
T Watanabe, M Enoki, S Sato, Y Honda, M Kuwahara, N Shirai, K Messner


The microbial ecology of treated birch stakes in a soil-bed
1983 - IRG/WP 1209
The microbial ecology of small birch stakes exposed in soil beds in two Fungus Cellars was investigated. Patterns of fungal colonisation and decay initiation are described in untreated birch and copper fluosilicate-treated stakes. Results are based on fungal isolation onto selective media and direct light microscopical observations of decay features. In untreated stakes there was a rapid build-up ...
C P Clubbe


Factors affecting decay rates in a fungus cellar
1985 - IRG/WP 2242
Birch and pine stakes treated with chromated copper arsenate and didecyldimethylammonium chloride were incubated in two soils in a "Fungus Cellar" test. At three month intervals, sets of stakes were inspected and assigned visual ratings or removed from the soil beds for weight loss determination. Preliminary results after nine months exposure have shown differences in the decay rates between birch...
P A McKaig


Degradation of woods by obligately anaerobic fungi
1990 - IRG/WP 1452
Little is known about the processes occurring during the degradation of wood by obligately anaerobic fungi. When woods from a range of hardwood species were incubated for 11 days with a strain of the Chytrid Neocallimastix frontalis many of the woods were measurably fermented; Fagus sylvatica (23%) was the most highly degraded. Microscopic examination of Populus tremuloides wood after incubation w...
K N Joblin, G E Naylor


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