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Enhancing Our Understanding of Brown Rot Mechanisms through Catalytic Pretreatment and Cellulase Cocktail
2018 - IRG/WP 18-10909
A catalytic mechanism, described as the “chelator-mediated Fenton” (CMF) mechanism, is proposed to mimic the non-enzymatic action of brown rot fungi. A CMF treatment was used together with an enzymatic cocktail to study how wood was deconstructed and solubilized. This was done in-part to determine if the treatment mimicked the action of brown rot fungi, but also to explore improved treatment p...
S Tabor, L Orjuela, D Contreras, G Alfredsen, J Jellison, S Renneckar, B Goodell


Preliminary Investigation into the Natural Decay Resistance of Nigerian Grown Hevea brasiliensis and Mitragyna ciliata wood to Phanerochaete chrysosporium White-Rot Fungus
2018 - IRG/WP 18-10919
Wood is an important and versatile structural building material that finds applications in numerous uses. However, wood is also a biological material vulnerable to degradation by microbial activities; this is especially true in the tropics. Following the scarcity of highly durable species from our natural forests and the introduction of so many lesser used/durable wood species into the booming tim...
J M Owoyemi, U O Emmanuel


Mechanism of nano metal fluorides against fungi
2018 - IRG/WP 18-30730
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredient in wood preservatives such as copper and boron. Wood specimens treated with fluoride sols of MgF2 and CaF2 have been tested against brown-rot fungi (Coniophora puteana and Rhodonia placenta) in accordance with EN 113 and against termites (...
S M Usmani, K Klutzny, I Stephan, T Hübert, E Kemnitz


FTIR-ATR monitoring of chemical changes of thermo-chemically modified beech wood degraded by brown-rot fungus
2018 - IRG/WP 18-40823
Wood chemical modification with lactic acid oligomers (OLA) has been reported to confer promising properties for wood outdoors applications. To better understand the lactic acid interaction with wood, chemical changes following biological degradation have been characterized with a battery of tests. Fourier transform infrared (FTIR) spectroscopy has been proved to be a valuable tool for studying fu...
C Grosse, M Noël, M-F Thevenon, P Gérardin


Recent findings clarifying the mechanism of brown-rot decay protection in acetylated wood
2018 - IRG/WP 18-40827
This paper summarizes the findings obtained during my PhD research over the past three years. The results provide insights which help to understand the enhanced brown-rot decay resistance of acetylated wood with particular emphasis on the role of water. The protection acetylation provides is derived from moisture exclusion within the acetylated cell wall, but the exact mechanism by which water is ...
G Beck


The biodegradation of creosote oil by various species of white rot fungi and bacteria isolated from the contaminated soil
2018 - IRG/WP 18-50334
The aim of the study was to determine biodegradation effectiveness of the selected polycyclic aromatic hydrocarbons (PAHs) contained in creosote oil, by two white rot fungal species (Bjerkandera adusta and Irpex lacteus) and three bacterial species (Pseudomonas putida, Pseudomonas sp. OS4 and Rahnella aquatilis). Bacteria were isolated from the soil contaminated with petroleum hydrocarbons from th...
M Sydow, J Zabielska-Matejuk, E Kaczorek, AStangierska, A Kropacz


Non-stochiometric oxidation and ROS generation promoted by guaiacol lignin structures and lignocelluose surfaces may be a component of brown rot fungal degradation mechanisms
2019 - IRG/WP 19-10937
Model guaiacol compounds representing lignin monomers, as well as DHP-lignin and wood flour of controlled particle size were used to assess iron reduction at the pH of the natural wood cell wall. All compounds functioned as electron donors for ferric iron, with the lignin monomers demonstrating capacity for non-stochiometric reduction of iron with multiple moles of ferric ion reduced per mole of l...
Y Tamarua, M Yoshidaa, L D Eltisb, B Goodell


Difference of wood decay manner between brown-rot species
2019 - IRG/WP 19-10938
Wood blocks (Cryptomeria japonica) which were decayed by a Polyporales white-rot fungus (Trametes versicolor) and brown-rot fungi (Fomitopsis palustris, F. pinicola, and Wolfiporia cocos), respectively, were analyzed by X-ray diffraction and infrared spectroscopic methods followed by multivariate analysis. In the analyses, the differences in the cellulose crystallinities and infrared spectral patt...
R Kondo, Yo Horikawa, S Nakaba, K Ando, M Yoshida


From fungal detoxification systems to wood durability in neotropical forests
2019 - IRG/WP 19-10946
White-rot fungi are able to mineralize all the wood components due in particular to an efficient detoxification system. We hypothesised that components of this detoxification systems, glutathione transferases, could be used as tools to explore the natural durability of neotropical wood species. Analysis of the interactions between six glutathione transferases of Trametes versicolor and extracts of...
T Perrot, G Salzet, N Amusant, J Duchene, E Gelhaye


Characterization of Pectinases from Brown-rot Fungus Fomitopsis palustris
2019 - IRG/WP 19-10951
Brown-rot fungi occur on softwood used as building materials and cause destructive breakdown of wood structure. Therefore, a more accurate understanding is important from the perspective of wood protection. Previous studies have reported that hyphae of brown-rot fungi go through bordered pits on tracheids when the fungi grow into softwood [Francis W.M.R.Schwarze (2007)], and torus existing in the ...
Y Tanaka, N Konno, T Suzuki, N Habu


Chemical composition, antitermite and antifungal activity of Dacryodes edulis oleoresin
2019 - IRG/WP 19-20653
Damages to wood structures and other cellulosic materials caused by wood destroying insects and fungi are estimated to several billions of dollars each year in the world. Among these, termites are considered as one of the most economically important pests for wooden structures. In the past, several wood protection chemicals like CCA, creosote, lindane or pentachlorophenol have been used. However, ...
W F Bedounguindzi, K Candelier, P E Engonga, Se Dumarcay, M-F Thevenon, P Gerardin


Hydroxyl accessibility - A brief review of deuterium exchange studies on modified wood
2019 - IRG/WP 19-20656
Wood modification imparts dimensional stability and decay resistance. The mechanism(s) by which this occurs are thought to be related to the bulking of the wood cell wall and subsequent moisture exclusion. Hence, quantification of accessible hydroxyl (OH) groups is of great interest when evaluating and increasing the understanding of the performance of modified wood. The aim of this study was to b...
G Beck, C Hill, G Alfredsen


Impact of water holding capacity and moisture content of soil substrates on the moisture content of wood in terrestrial microcosms
2019 - IRG/WP 19-20662
Terrestrial microcosms (TMC) are frequently used for testing the durability of wood and wood-based materials as well as the protective effectiveness of wood preservatives. In contrary to experiments in soil ecology sciences, the experimental set-up is usually rather simple. However, for service life prediction of wood exposed in ground, it is of immanent interest to better understand the different...
C Brischke, F L Wegener


Biocidic effect of nano metal fluorides against brown-rot fungi
2019 - IRG/WP 19-30732
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredients in wood preservatives such as copper and boron. The effectiveness of nano metal fluorides was compared to sodium fluoride treated wood samples. Wood specimens treated with homo-dispersed sols of MgF2 and CaF2 were tested against brown-rot...
S M Usmani, K Klutzny, I Stephan, E Kemnitz


Relative efficacy of various oxine copper formulations against brown-rot fungi
2019 - IRG/WP 19-30741
This paper investigates the relative resistance of three different oxine copper formulations (oil-borne, water-borne, water-borne with pH-adjustment) as a preservative treatment for wood against brown-rot fungi. Impregnated southern pine sapwood cubes were exposed to R. placenta and G. trabeum cultures in a soil-block test. After eight weeks, the weight losses of the cubes were examined in relatio...
M Petruch, J Lloyd, A Taylor


The impact of additional interior layers to the moisture dry-out and mould growth risk of CLT walls
2019 - IRG/WP 19-40876
The objective of this study was intended to determine the maximum allowable initial moisture content for CLT walls having both exterior and interior thermal insulation. A laboratory test was conducted, for which four test walls with two different insulation solutions and two different initial moisture contents were built. Based on the test results a simulation model was configured and simulations ...
V Kukk, J Kers, T Kalamees


Pigment production by the spalting fungus Scytalidium ganodermophthorum and its industry potential
2020 - IRG/WP 20-10957
Scytalidium ganodermophthorum is best known as a pathogen of cultivated mushrooms, with infected cultures turning yellow in color. The fungus is also used in the art form known as ‘spalting’ to produce yellow, green, and purple colors in wood for decorative purposes. Wood colored by fungus in this manner has been traditionally used in the creation of fine art and woodworking since the 16th cen...
R C Van Court, P Vega Gutierrez, S C Robinson


Different levels of acetylation lead to groupwise upregulation of non-enzymatic wood degradation genes of Rhodonia placenta during initial brown-rot decay
2020 - IRG/WP 20-10958
Rhodonia placenta, often used as a model fungus to represent brown rot fungi, uses a two-stepped degradation mechanism to degrade wood. Regarding the overcoming of wood protection systems the initial degradation phase seems to be the crucial point. A new laboratory test enables the separation of the non-enzymatic oxidative and the enzymatic degradation phases, which has previously been proven chal...
M Kölle, R Ringman, A Pilgård


FTIR analysis of wood blocks decayed by brown-rot fungi
2020 - IRG/WP 20-10959
Calibration curves of the relative lignin contents in Cryptomeria japonica decayed by brown-rot fungi were developed with Infrared Spectroscopy and Klason technique. First, wood decay test was conducted using brown-rot fungi (Fomitopsis palusris, F. pinicola, Wolfiporia cocos, Gloeophyllum trabeum, and Neolentinus suffrutescens) and white-rot fungus (Trametes versicolor) was used as a comparison. ...
R Kondo, Y Horikawa, R Kose, M Yoshida


Durability of acetylated Radiata pine: Laboratory tests and performance in practice
2020 - IRG/WP 20-40899
Wood acetylation is an established process to enhance biological durability and dimensional stability of lower valuable wood species without the use of biocides. Acetylated Radiata pine (ACCOYA® wood) has been on the market for more than 10 years now, starting in 2007. Numerous lab tests have confirmed the high durability of acetylated Radiata pine. Here, additional data with four Radiata pine ...
K Jacobs, W Scheiding, B Weiß


The iron reduction by chemical components of wood blocks decayed by wood rotting fungi
2021 - IRG/WP 21-10979
Brown-rot fungi, a group of wood rotting fungi, is well known to be one of major microorganisms that cause the deterioration of wooden buildings in Japan and have been considered to use chelator-mediated Fenton (CMF) reaction in concert with hydrolytic and redox enzymes for degradation of wood cell wall. CMF can be described as a non-enzymatic degradation system that utilizes hydroxyl radicals pro...
R R Kondo, Y Horikawa, K Ando, B Goodell, M Yoshida


Mechanical and biological durability properties against soft-rot and subterranean termite in the field (grave-yard test) of beech wood impregnated with different derivatives of glycerol or polyglycerol and maleic anhydride followed by thermal modification in an opened or in a closed system
2021 - IRG/WP 21-40917
This paper presents mechanical and biological durability properties in soil beg test (soft-rot test) and field test (grave-yard test) against subterranean termite of the wood modified with an aqueous vinylic derivative of glycerol/polyglycerol or maleic anhydride cured in an opened or in a closed system. Wood modification was performed through impregnation of European beech (Fagus sylvatica) with ...
M Mubarok, H Militz, S Dumarcay, I W Darmawan, Y S Hadi, P Gerardin


Decay capacity and degradation patterns of Xylaria hypoxylon on different wood species
2022 - IRG/WP 22-10985
A host of physical and environmental factors may influence fungal decay including the wood substrate, temperature, moisture, oxygen, light, pH, and nitrogen. Understanding the effects of these factors on fungal decay is important for the effective utilization of wood decay fungi in biotechnological processes and for understanding the role of these organisms in global carbon cycling. The ascomycete...
E Bari, G Daniel, A Singh, J J Morrell


Changes in topochemistry and mechanical properties of Beech (Fagus orientalis L.) by natural fungus infestation
2022 - IRG/WP 22-10986
Beech is one of the major hardwood species in Europe. It is, however, highly susceptible to fungal attack both in the fresh state and during the storage. Understanding the alteration in chemical and mechanical properties of beech wood during the initial degradation state can lead to improved raw material utilization. Therefore, UV-microspectrophotometer (UMSP) and High-Performance Liquid Chromatog...
H Sivrikaya, M Rehbein, F Divos, S Adamopoulos, R Hosseinpourpia


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to dete...
E E Soytürk, S N Kartal


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