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The role of oxidation in wood degradation by brown-rot fung
1992 - IRG/WP 92-1562
Brown-rot fungi are suggested to degrade cellulose by oxidation with hydrogen radicalsformed eg. in the conversion of hydrogen peroxide in the Fenton type reactions. The stuctural changes in the holocellulose in wood induced by Fenton's reaction on wood components are very similar to those caused by brown-rot fungi. In this work the effect of the Fenton reaction on wood components was stu...
A-C Ritschkoff, J Pere, J Buchert, L Viikari


Alkaline building materials and controlled moisture conditions as causes for dry rot Serpula lacrymans growing only in houses
1985 - IRG/WP 1272
Dry rot Serpula lacrymans ( Fr.) S.F. Gray is commonly found in houses, though never with certainly in nature, like other wood destroying fungi which grow both indoors and outdoors. In investigating series of dry rot instances it was shown that this fungus is always found in covered places, close to a moisture source, the distance being from 0 a maximum of 600 cm. Owing to the dry rot has been abl...
J Bech-Andersen


Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing
2004 - IRG/WP 04-10512
Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample....
U Råberg, N Högberg, C J Land


Old and new facts on the dry rot fungus Serpula lacrymans
1991 - IRG/WP 1470
The article collates some of the recent literature on the biology of the dry rot fungus Serpula lacrymans. The fungus can grow at 28°C, and maximum wood moisture is above 55%. Serpula Iacrymans degrades crystalline cellulose. The intensive production of extracellular oxalic acid is neutralized by calcium and iron. There is considerable variation among the strains with regard to factors such as gr...
O Schmidt, U Moreth-Kebernik


Preliminary studies of the performance of iron chelators as inhibitors of brown rot (Coniophora puteana) attack
1996 - IRG/WP 96-10185
This paper describes experiments to examine the proposal that the presence of iron is essential for brown rot fungi to utilize hydroxyl radicals remote from the hyphae as a means of converting the wood into a food source. reliminary test results are presented from trials using three different iron chelators impregnated into Scots pine (Pinus sylvestris) sapwood blocks. Their relative effects on th...
E D Suttie, R J Orsler, P M Wood


Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


The high decay resistance in the sapwood of the naturally durable Malaysian hardwood Belian (Eusideroxylon zwageri)
2001 - IRG/WP 01-10410
It has long been assumed that the observed natural durability of the heartwood in certain timbers is perhaps associated with a relatively lower decay susceptibility also of the sapwood of these species. While the heartwood of Belian is reputedly highly decay resistant among the tropical hardwoods of Southeast Asia, laboratory decay tests reported in this paper have also confirmed the high decay re...
A A H Wong, A P Singh


Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison


The Effect on Biological and Moisture Resistance of Epichlorohydrin Chemically Modified Wood
2002 - IRG/WP 02-40224
Southern pine solid wood and fiber were chemically modified with epichlorohydrin to help in understanding the role of moisture in the mechanism of biological effectiveness of chemically modified wood. The solid wood had weight gains from 11% to 34%, while the fiber had weight gains from 9% to 75%. After modification, part of the specimens were water leached for 2 weeks or extracted for 2 hours ...
R E Ibach, B-G Lee


Microscopic analysis of southern pine utility pole core segments
1992 - IRG/WP 92-1542
Fifteen macroscopic core descriptive categories were evaluated by microscopic analysis for accuracy in detecting decay and judging decay severity. Five categories were good indicators of decay as decay was detected in 28 of 48 samples. Five categories were possible indicators of early decay as decay was detected in 6 of 40 core segments and an additional 18 contained hyphae. The remaining five cat...
S E Anagnost, C J K Wang, M Wright


The Copper Tolerance of Mycelium vs. Spores for Two Brown Rot Fungi
2002 - IRG/WP 02-10422
The copper tolerance of two brown rot fungi, Gloeophyllum sepiarium and Oligoporus placentus, has been tested using both mycelia and basidiospores as inoculum sources. Mycelial growth of O. placentus on agar medium was shown to be much more tolerant to copper (as copper sulfate) than that of G. sepiarium. However, there was no difference in copper tolerance of basidiospores between the two specie...
S Choi, J N R Ruddick, P I Morris


Dimensional stabilization and decay resistance of wood treated with brown-rotted lignin and copper sulfate
1990 - IRG/WP 3608
The objective of this study was to evaluate the potential usefulness of brown-rotted lignin (BRL) as a dimensional stabilization and copper complexing agent for wood treatment. For dimensional stabilization, aqueous solutions of the lignin extract were combined with either copper sulfate, glyoxal or other additives. Anti-shrink efficiency (ASE) values as high as 42% were obtained with wood treated...
L Jin, D D Nicholas, T Schultz


Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse


Growth of the copper tolerant brown rot fungus Antrodia vaillantii on different substrates
1995 - IRG/WP 95-10121
In recent years the copper tolerant brown rot fungus Antrodia vaillantii caused severe damages on impregnated wood in ground contact. The pattern of decay gave the impression that impregnated wood was even more severely attacked than unimpregnated. To investigate this question more closely laboratory tests were carried out. In a "choice test" Antrodia vaillantii grew preferably towards CC-impregna...
H Leithoff, I Stephan, M-T Lenz, R-D Peek


Influence of variable lignin content amongst hardwoods on soft-rot susceptibility and performance of CCA preservative
1982 - IRG/WP 1151
This paper presents the outline of an argument, based on both new and established data, which relates varying lignin content to variation of soft-rot susceptibility between hardwood species and of performance of CCA preservatives in controlling soft-rot. Although more data need to be generated to prove this relationship, sufficient evidence exists for the hypothesis to be given close attention, pa...
J A Butcher, T Nilsson


Effect of moisture and cellulosic substrate on decay of hardwoods by soft-rot fungi
1982 - IRG/WP 2173
The development by Forintek Canada Corp. of standard test procedures for laboratory preservative evaluations using soft-rot fungi was requested by the American Wood Preservers' Association. In Europe, through cooperative studies in the International Research Group on Wood Preservation, several attempts have been made to produce a standard procedure, but variability in results obtained bet...
A Byrne, R S Smith


Practical experiments with Boracol 10 Rh used as a fungicide in the repair process after attack by the dry rot fungus (Serpula lacrymans)
1987 - IRG/WP 3458
The test is carried out in an old house in Nyhavn, Copenhagen. The roof and the walls close to were heavily attacked by dry rot fungus (Serpula lacrymans) because damage to the zinc covering the frontspice were letting in water to the wood and wall construction. An attack by the dry rot fungus is more complicated to repair compared with other wood destroying fungi because beside the damage on wood...
J Bech-Andersen


The effect of of sorbitol on the decay of boric acid treated Scots pine
1991 - IRG/WP 1509
The tetra-hydroxy borate ion is known to undergo complexation with polyols of biological importance and probably protects treated timber by this action. The inhibitory effect of boric acid upon fungal growth and dehydrogenases has been shown to be prevented by the addition of certain polyols. This work was extended, with a study of the effects of a polyol on the performance of boric acid in treate...
J D Lloyd, D J Dickinson, R J Murphy


Soft rot decay in CCA treated eucalypts in Queensland - A comment
1986 - IRG/WP 1301
A survey has been completed concerned with the distribution and severity of groundline soft rot decay in the CCA treated sapwood of eucalypt poles in Queensland. The survey encountered some 1000 poles of which 55% were slightly, 28% moderately and 17% severely decayed. Soft rot decay was more severe in urban than rural locations. Embedment of poles in concrete resulted in severe soft rot. No signi...
L E Leightley


The effect of a chelator mediated fenton system on activation of TMP fibres and decolorization of synthesized dyes
2004 - IRG/WP 04-50223
The purpose of this work is to improve our current knowledge of the non-enzymatic mechanisms involved in the brown rot degradation of wood, but also to study the potential applications of a chelator-mediated Fenton system in activation of wood fibers and decolorization of synthesized dyes. In this work, Electron Spin Resonance (ESR) spin-trapping techniques were used to study the generation of hyd...
Yuhui Qian, B Goodell, J Jellison


Solid state NMR study on pine wood degraded by brown rot fungus Gloeophyllum trabeum
1994 - IRG/WP 94-10067
Blocks of Pinus koraiensis wood were degraded by Gloeophyllum trabeum and examined by solid-state 13C NMR spectroscopy. Chemical compositions were estimated from relative signal aras. There was no evidence for preferential degradation of the noncrystalline cellulose which accounted for about 4% by weight of the wood. Loss of partly ordered cellulose on crystal surfaces exceeded the loss of crystal...
Yoon Soo Kim, R Newman


Redox regulation of enzyme activity during wood decay
1996 - IRG/WP 96-10172
A potential strategy in the search for alternative wood preservatives against fungal decay is to target the extracellular wood-decay process itself, rather than the decay organisms. This presents novel targets for selective disruption and possibly without the broad-spectrum toxicity associated with conventional wood preservatives. The enzymes of white rot decay are mechanistically diverse (e.g. hy...
P J Kersten, B Kurek, J W Whittaker


Application of non-destructive techniques (durometric and ultrasonic) to evaluate the degradation of woods in service by Gloeophyllum trabeum
2001 - IRG/WP 01-20220
In order to establish the degree of degradation of wood used in construction, it is necessary to employ non-destructive methods (ultrasonic, durometric, densitometric). In this study, ultrasonic and Pilodyn durometric techniques have been applied to try to establish parameters of relationship between the values obtained by both, for their immediate application to wood in service. As a method of re...
M T De Troya, L Palaia, A Navarrete, V Galvañ, R Molina, A Guijarro, J Camacho


The effect of initial moisture content on wood decay at different levels of gaseous oxygen concentration
1999 - IRG/WP 99-10316
The influence of initial moisture content on wood decay at different levels of atmospheric oxygen concentration was studied. Three fungi, Coriolus versicolor a white rot, Coniophora puteana a brown rot, and Chaetomium globosum a soft rot, were chosen. The mycelia of the fungi were inoculated on mini blocks of Fagus sylvatica (beech) and Pinus sylvestris (Scots pine). Incubations were under four le...
S M Kazemi, D J Dickinson, R J Murphy


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