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Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison


Preservative ability of wood to be fixed hydroxyl apatite substituted for antimicrobial metals
2001 - IRG/WP 01-30272
We succeeded in forming hydroxy apatite (HAp) in wood. HAp is non-toxicity and safe. Preservative ability of the wood, which substituted one part of Ca of constituent element of this HA p for antibacterial metals was measured. When Ca was substituted for Ag or Zn, mass loss in decay by brown-rot fungus F. palustris was restrained in about 50% (Ag) ~30% (Zn) of value of control specimen. However, w...
Y Haruhiko, I Sumaru


Enhanced biodegradation of cocopeat by soft rot fungi
1998 - IRG/WP 98-10276
Biodegradation of cocopeat (coir dust) was enhanced by the addition of nitrogen (N) fertilizer and inoculation with the soft rot fungus Chaetomium globosum. The N and fungally treated cocopeats had a greater percentage weight loss (27%) after 3 months compared with 7% weight loss without the added fungi and N fertilizer. In addition, their hemicellulose, cellulose and lignin contents were greatly ...
P Y Yau, R J Murphy


Protection of southern pine using N,N-Napthaloylhydroxylamine: Field tests, soft-rot cellars and aquatic bioassay leach testing
1999 - IRG/WP 99-30204
Recent environmental restrictions are limiting the use of broad-spectrum biocides for wood preservation. There is an urgent need for new, sharply targeted, environmentally benign wood preservatives. N'N-Napthaloylhydroxylamine (NHA), a water-soluble calcium-precipitating agent, has been shown to inhibit decay by brown-rot and white-rot fungi in soil-block tests and prevent damage by Easte...
D M Crawford, F Green III


Soft rot
1978 - IRG/WP 179
Soft rot decay of treated wood is examined with special reference to hardwoods treated with CCA. Factors which adversely affect the chances of protection of hardwoods against soft rot are discussed. The ratio of the volume of the fibre cell wall to the volume of the fibre lumen is presented as a major factor influencing final preservative concentration in the fibre cell wall, the major strength co...
C R Levy


Influence of CCA and TnBTO on wood decay, under different oxygen levels and various initial moisture content
2001 - IRG/WP 01-10415
Fagus sylvatica (beech) and Pinus sylvestris (Scots pine) were impregnated by TnBTO (tributyl-tin oxide) and CCA (chromated copper arsenate). In wood blocks two levels of moisture content (50% and 100% of saturation point) were used. Three levels of oxygen concentrations (10, 21, and 50%) were chosen. Treated and untreated wood were exposed to three fungi (C. versicolor a white rot, C. puteana, a ...
S M Kazemi, R J Murphy, D J Dickinson


Molecular studies on house rot fungi by RAPD-PCR
1997 - IRG/WP 97-10195
For genetic information and in view as a possible diagnosis method various isolates of the dry rot fungus, Serpula lacrymans, the wild merulius, S. himantioides, and the cellar fungus, Coniophora puteana, from Asia, Europe and USA were investigated by the polymerase chain reaction (PCR) based technique of randomly amplified polymorphic DNA (RAPD). The banding patterns obtained revealed for S. lacr...
O Schmidt, U Moreth


Decay resistance of densified ammonia-plasticized stems of oil palm (Elaeis guineensis)
1991 - IRG/WP 3673
When wafers of oil palm stems (Elaeis guineensis) were plasticized with 28% aqueous ammonia and immediately compressed mechanically, the treated material, gained 73% in basic density (average basic density, 0.695 g/cm³), and was highly resistant to decay by wood rot basidiomycetes. Compared with the control specimens (density, 0.403 g/cm³), resistance to decay of the densified specimens by Corio...
A H H Wong, M P Koh


Detection of brown-rot antigens in southern pine
1996 - IRG/WP 96-20090
Brown rot fungal antigens were detected by particle capture immunoassay (PCI) in southern pine 2 x 4's beyond visible or culturable hyphal growth. Further analysis of test samples revealed changes along the 2 x 4's that could be grouped into zones. Zone 1, the point of inoculation through 6 cm, showed low pH, measurable oxalic acid, high moisture, and high protein. Zone 2, throug...
C A Clausen


Hemicellulosic induction of oxalic acid in Postia placenta
1994 - IRG/WP 94-10060
Most studies suggest that enzymes produced by brown-rot fungi are too large to penetrate sound wood structures, even after decay begins. Thus, nonenzymatic agents have been proposed to initiate brown-rot decay. We have reported that the brown-rot fungus Postia placenta MAD-698 initiates a 2-fold decrease in wood pH within 7 days of colonization which is mediated by production of oxalic acid. Strai...
F Green III, M Larsen, T L Highley


Laboratory testing of wood natural durability - In soil-bed assays
1998 - IRG/WP 98-20141
Laboratory methods for assessing wood decay resistance are being investigated in the framework of an ongoing European research project. This paper summarizes the main results obtained for soil-bed tests based on the European prestandard ENV 807-Test 2. The data suggest that this testing methodology is suitable for evaluating the natural durability of timber species in soil-contact under conditions...
R Sierra-Alvarez, I Le Bayon, J K Carey, I Stephan, J Van Acker, M Grinda, G Kleist, H Militz, R-D Peek


Tolerance of Wood Decay Fungi to Commercial Copper Based Wood Preservatives
2002 - IRG/WP 02-30291
Due to the use of copper based preservatives like CCB or CCA for more than a century, copper tolerant fungi have appeared in some European countries in recent times. It is therefore important to find out whether this phenomenon is specific for only classical copper ingredients, or generally for all copper based formulation. Thus, we tested the tolerance of three commercial copper based pres...
F Pohleven, M Humar, S A Amartey, J Benedik


Decay resistance of Platanus acerifolia wood against White Rot Fungi (Coriolus versicolor)
2002 - IRG/WP 02-10431
Degradation of the lignocellulose complex in wood varies on the microorganism causing decay and chemical composition of wood. The weight loss of Platanus acerifolia wood after decay by white rot fungus (Coriolus versicolor) for period 16 weeks were evaluated according to European standard EN 113 and beech wood as a control. The samples were collected in October 2000 from Nantes, France. The aim of...
M Irmouli, O Duchelier, N Ayadi, B Charrier


The long road to understanding brown-rot decay. A view from the ditch
1995 - IRG/WP 95-10101
Interest in understanding how brown-rot fungi decay wood has received increasing interest in recent years because of a need to identify novel targets that can be inhibited for the next generation of antifungal wood preservatives. Brown-rot fungi are unique in that they can degrade holocellulose (cellulose and hemicellulose) in wood without first removing the lignin. Furthermore, they degrade holoc...
F Green III, T L Highley


Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


Utilization of coconut timber from north Sulawesi, Indonesia. Part 1: Durability
1994 - IRG/WP 94-30044
Coconut timber from non-productive plantations is a byproduct of an agricultural crop and, by the same token, constitutes a renewable resource which may serve as a complement to or, at least in part, as a substitute for traditional timbers in local markets. Its more extensive utilization is expected to contribute to the conservation of tropical rain forests. Export of coconut timber and/or wood pr...
R-D Peek


The effectiveness of three tributyltin compounds in beech against soft rot fungi using a soil-bed technique: A preliminary report
1986 - IRG/WP 3390
The effectiveness, towards soft rot fungi, of bis (tributyltin) oxide, tributyltin ethanesulphonate (TBTES) and benzyltributylammonium tributyldichlorostannate in both leached and unleached beech stakes is compared to that of a CCA preservative (Tanalith CT 106), using a soilbed technique. In addition, the effect of test solvent (water or toluene) on the performance of TBTES is evaluated. Total ti...
R Hill, A H Chapman, B Patel, A Samuel, J K Carey


Performance of preservative-treated wood not in ground contact
1975 - IRG/WP 254
Experimental sash units of Pinus strobus L. and Pinus resinosa Ait. wood were removed from a test fence at Ottawa, Canada after outdoor exposure of over 20 years. The units were superficially treated, prior to painting, with preservatives containing either copper naphthenate, pentachlorophenol or a combination of zinc naphthenate and pentachlorophenol. Results indicated that all treatments were ef...
J K Shields, J Krzyzewski


Premature failure of CCA treated vineyard posts from brown rot
1993 - IRG/WP 93-10016
A survey of CCA (AWPA Type C) treated posts used as grape vine trellis supports, to determine the extent and type of decay was carried out at a terraced vineyard in the Hawkes Bay region of the North Island of New Zealand. Of 357 posts examined out of a total of 970 in the vineyard, 47% had moderate to severe decay or had failed, 20% had established decay, 10% had a trace of decay and 23% had no s...
R N Wakeling, A P Singh


Lab and field test results for wood treated with polymeric alkylphenol polysulfide
2001 - IRG/WP 01-30268
A new wood preservative based on polymeric alkyphenol polysulfide (PXTS-A) is being evaluated in a series of laboratory and field tests and the results continue to show promise. A modified formulation (PXTS-B), which contains additives to reduce the viscosity, is being evaluated along with the original formulation. Soil block decay tests comparing PXTS-B with CCA-C indicate that PXTS-B is equally ...
J Goswami, A Abramson, R Buff, D D Nicholas, T Schultz


Bioremediation of surfactant contaminated waste
1996 - IRG/WP 96-50070
The objective of this work was to determine the potential of fungi as agents for the bioremediation of wastes (particularly wood and soil) contaminated with quaternary ammonium compounds (QACs). Until now only bacteria have been investigated for this purpose. Tolerant strains of Gliocladium roseum and Verticillium bulbillosum were studied for their ability to degrade the following QACs: didecyldim...
J L Bürgel, J Dubois, J N R Ruddick


Durability aspects of (hydro)thermal treated wood
2000 - IRG/WP 00-40160
Samples of several wood species were treated in a two steps process, subsequently hydrothermal and dry heat-treated, by the so-called PLATO-process and analysed for their resistance against fungal attack. Both PLATO-treated and dry heat-treated specimen were prepared and analysed, in order to study the influence of moisture during hydrothermal treatment of wood. The resistance against all of the s...
B F Tjeerdsma, M Stevens, H Militz


The role of oxidation in wood degradation by brown-rot fung
1992 - IRG/WP 92-1562
Brown-rot fungi are suggested to degrade cellulose by oxidation with hydrogen radicalsformed eg. in the conversion of hydrogen peroxide in the Fenton type reactions. The stuctural changes in the holocellulose in wood induced by Fenton's reaction on wood components are very similar to those caused by brown-rot fungi. In this work the effect of the Fenton reaction on wood components was stu...
A-C Ritschkoff, J Pere, J Buchert, L Viikari


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