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Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


Microbial decay in an extremely durable Malaysian hardwood Belian (Eusideroxylon zwageri) - an overview
1997 - IRG/WP 97-10216
The heartwood of Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods, and is therefore a choice timber for soilcontact use in the humid tropics such as untreated telecommunication poles, fence posts and railway sleepers. The high microbial resistance of the wood is evidenced by the mere superficial degradation observed in some pole samples after 20 yea...
A H H Wong, A P Singh


Assessing the importance of degradation mechanisms on the loss of effectiveness of wood preservatives
2000 - IRG/WP 00-20193
Accelerated ageing systems developed for application to samples in the laboratory prior to biological tests, should reflect those natural deterioration processes that are likely to occur in the hazard classes defined in EN 335-1. Losses through evaporation or the effects of leaching have been recognised, however their importance, relative to other mechanisms has not been quantified. Degradation me...
E D Suttie, R J Orsler, T Dearling


Bacterial degradation of Pinus radiata compression wood
1996 - IRG/WP 96-10153
An inspection of twelve year old, CCA-treated Pinus radiata wood from an industrial cooling tower showed extensive surface decay of wood. Electron microscopic examination of decaying compression wood indicated that compression wood cells were attacked exclusively by bacteria, which were of erosion and tunnelling types. As compared to the normal wood, compression wood appeared to be more resistance...
A P Singh, R N Wakeling


Dimensional stabilization and decay resistance of wood treated with brown-rotted lignin and copper sulfate
1990 - IRG/WP 3608
The objective of this study was to evaluate the potential usefulness of brown-rotted lignin (BRL) as a dimensional stabilization and copper complexing agent for wood treatment. For dimensional stabilization, aqueous solutions of the lignin extract were combined with either copper sulfate, glyoxal or other additives. Anti-shrink efficiency (ASE) values as high as 42% were obtained with wood treated...
L Jin, D D Nicholas, T Schultz


Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner


Breakdown of cellulose derivatives by cellulolytic enzymes. I. Action of fungal ß-glucosidases toward substituted PNP ß-D-glucopyranosides
1998 - IRG/WP 98-10281
The action of fungal ß-glucosidases on the methyl derivatives of p-nitrophenyl (PNP) ß-D-glucopyranoside, which were regioselectively substituted at 0-2, 0-3, 0-4 and 0-6 positions was studied. Several ß-glucosidases from brown-rot, white-rot, and soft-rot fungi and almond were used for the study. These ß-glucosidases did not act on the 2, 3, and 4-0-methyl derivatives, while the 6-0-methyl on...
T Nishimura, I Momohara, M Ishihara


Fruitbody formation and basidiosporogenesis by the white rot fungus Pycnoporus cinnabarinus
1988 - IRG/WP 1348
Conditions for fruitbody formation by the white rot fungus Pycnoporus cinnabarinus in vitro were outlined. A marked difference in substrate requirements in comparison to previously tested brown rot fungi was revealed. Agar media containing Walseth cellulose and NH4 tartrate at 1 g/l permit profound production of basidiospores particularly at 15°C but only under light treatment. Higher NH4 tartrat...
J Bjurman


Influence of variable lignin content amongst hardwoods on soft-rot susceptibility and performance of CCA preservative
1982 - IRG/WP 1151
This paper presents the outline of an argument, based on both new and established data, which relates varying lignin content to variation of soft-rot susceptibility between hardwood species and of performance of CCA preservatives in controlling soft-rot. Although more data need to be generated to prove this relationship, sufficient evidence exists for the hypothesis to be given close attention, pa...
J A Butcher, T Nilsson


Experiments on the degradation of tributyltin oxide: A progress report
1984 - IRG/WP 3287
A variety of experiments designed to assess the chemical and physical factors affecting the degradation of tributyltin oxide in treated timber are described. Simple procedures in which temperature and oxygen availability were increased in the presence of wood and water suggest that the wood itself was of prime importance. Attempts to decrease degradation with antioxidants were unsuccessful but led...
R J Orsler, G E Holland


Deterioration of wood viewed from iron nail
1991 - IRG/WP 2368
Deterioration of nail was graded by a five - rank numerical - rating. The rating of nail moisture content and decay of wood were inspected in a mortal-wall of 34-year-old house. The wood was mostly in decay, when the rating of the nail was above about 4, and the moisture content was above about 20%. Nailed wood specimens kept in several humidity room of 20°C for 4 years showed that the rating of ...
H Imamura


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


Solid state NMR study on pine wood degraded by brown rot fungus Gloeophyllum trabeum
1994 - IRG/WP 94-10067
Blocks of Pinus koraiensis wood were degraded by Gloeophyllum trabeum and examined by solid-state 13C NMR spectroscopy. Chemical compositions were estimated from relative signal aras. There was no evidence for preferential degradation of the noncrystalline cellulose which accounted for about 4% by weight of the wood. Loss of partly ordered cellulose on crystal surfaces exceeded the loss of crystal...
Yoon Soo Kim, R Newman


The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe


Termiticide degradation in Mississippi field tests
1994 - IRG/WP 94-30052
Degradation rates of some currently marketed United States termiticides are currently being studied. Separate quantities of soil were each treated at lowest label rates with termiticide, and placed in trenches along the inside and outside of concrete walls. Composite soil samples, each consisting of four soil cores, were collected after 1, 30, 60, 120, 180, 240, and 365 days, and again at 2 and 3 ...
B M Kard


Application of non-destructive techniques (durometric and ultrasonic) to evaluate the degradation of woods in service by Gloeophyllum trabeum
2001 - IRG/WP 01-20220
In order to establish the degree of degradation of wood used in construction, it is necessary to employ non-destructive methods (ultrasonic, durometric, densitometric). In this study, ultrasonic and Pilodyn durometric techniques have been applied to try to establish parameters of relationship between the values obtained by both, for their immediate application to wood in service. As a method of re...
M T De Troya, L Palaia, A Navarrete, V Galvañ, R Molina, A Guijarro, J Camacho


Ultrastructure of Prumnopitys ferruginea wood from a buried forest in New Zealand
1991 - IRG/WP 1489
The buried wood of Prumnopitys ferruginea, a New Zealand native tree, was examined by scanning and transmission electron microscopy to investigate the extent to which the wood had deteriorated and the cause of its deterioration. Examinations showed that some deterioration of wood had occurred. Microscopy revealed a decay pattern similar to that produced by cavitation bacteria. Lenticular cavities ...
A P Singh, T Nilsson, G F Daniel


16S rRNA Analysis of the Bacteria Associated with Biocide Degradation
2004 - IRG/WP 04-10543
The bacterially mediated degradation of the new anti-fungal biocide, bethoxazin, was studied in vitro, by means of 16S rRNA PCR-amplification and cloning techniques. Woodblocks impregnated with a subtoxic concentration of bethoxazin were incubated in compost, and the micro-organisms associated with the wood after 6 and 12 weeks was studied. It was found that after 6 weeks the wood contained a la...
D F Wallace, D J Dickinson


Evidence for actinomycete degradation of wood cell walls
1990 - IRG/WP 1444
Several unique patterns of degradation occurring in wood cell walls have been observed in wooden stakes inserted in unsterile soil in the laboratory. Some of the patterns have also been observed in coniferous wood taken from forest floors. All the observed attack types occur within wood cell walls, mainly within the S2 layer. Attack is characterised by channels of varying diameter or small fusifor...
T Nilsson, G F Daniel, S L Bardage


The influence of staining fungi on the decay resistance of wood treated with alkylammonium compounds
1984 - IRG/WP 3308
Although found to be very effective in laboratory tests, alkylammonium compounds (AAC's) have failed to perform as well in field stake tests. Examination of leachability showed that this was not the cause. The present study investigated the possibility that staining fungi, (which have been observed to rapidly infect the field stakes), degrade the AAC wood preservative. Soil-blocks were tr...
J N R Ruddick


Wood and filter paper degradation, phenol oxidase and one-electron oxidation activities by the white rot fungus Ceriporiopsis subvermispora
2003 - IRG/WP 03-10486
The activities of one-electron oxidation and phenol oxidase during incubation of cultures of the white-rot basidiomycete Ceriporiopsis subvermispora containing either glucose or wood were periodically measured. Further, the degradation activities against wood and filter paper were examined during the course of cultivation. Weight losses of Japanese beech wood and Japanese cedar wood after 12 wee...
H Tanaka, S Itakura, A Enoki


Chemical and biological investigations of double-vacuum treated windows after 5 years in service
1983 - IRG/WP 3219
In 1980 The Swedish Wood Preservation Institute initiated an investigation to study the degradation of TBTO and possible fungal attack in double-vacuum treated window joinery in service during 5 years. A hospital in Gothenburg was chosen that was built during 1969 to 1976. Both untreated and double-vacuum treated windows of Pinus sylvestris were used. A brown alkyl oil type paint (Nordsjö system ...
J Jermer, M-L Edlund, B Henningsson, W Hintze, S V Ohlsson


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


Degradation of polycyclic aromatic hydrocarbons by laccase
1998 - IRG/WP 98-10267
The degradation of polycyclic aromatic hydrocarbons (PAH) by laccase was investigated. Laccase catalyzed oxidation of phenanthrene, solubilized in the surfactant Tween 20, exhibited an absolute requirement for the mediator compound 1-hydroxybenzotriazole. When Tween 80 was used as the solubilizing agent, the oxidation rates were more than six times higher as compared to Tween 20. Using the thiobar...
S Böhmer, E Srebotnik, K Messner


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