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Soft rot and bacterial decay in preservative treated eucalypt power transmission poles
1982 - IRG/WP 1155
Bacterial type decay was observed in CCA and PCP treated eucalypt power transmission poles. Detailed observations made with the SEM revealed bacterial colonisation and decay, especially in fibres. Plug samples taken from poles throughout Queensland were examined for preservative retention and presence of soft-rot decay. The severity of decay was different according to location, retention and speci...
L E Leightley


Cytochemical localization of hydrogen peroxide in brown rot fungus Tyromyces palustris by cerium chloride technique
1999 - IRG/WP 99-10299
Cerium chloride (CeCl3) was used to localize H2O2 cytochemically for studying relationship between ultrastructural and functional characteristics of cellulose degradation by brown rot fungi. This technique proved very useful in localizing discrete electron-densereactionproducts at high resolution with minimal nonspecific deposition. The cytochemical localization of extracellular H2O2 by CeCl3 usin...
Yoon Soo Kim, Seung-Gon Wi


Some thoughts on the future strategy for eradicating Serpula lacrymans from a building
1989 - IRG/WP 1405
We now have a clear view of the mechanism of translocation of nutrients in the mycelium of Serpula lacrymans which is one of the physiological processes underlying the remarkable capacity of this fungus to spread through a building. Here the elements of the mechanism of translocation are dissected out to suggest avenues which might be followed in the search for new ways for eradicating the fungus ...
D H Jennings


Collaborative soft rot tests
1981 - IRG/WP 2151
There appears to be need for reconsideration of attitudes towards Standardisation of laboratory tests for determining the toxicity of preservatives to soft rot fungi. The original approach was that a limited number of test methods were available and that collaborative effort would determine the suitability of the methods for Standardisation. It has been established so far that pure culture testing...
J G Savory


Fungus cellar and stake tests with tall oil derivatives. Progress Report after 5 years' testing
1987 - IRG/WP 3442
Two derivatives of tall oils have been tested for five years in fungus cellar and stake tests. The samples were relatively quickly attacked on the surface by decay fungi, mainly soft rot, but the decay has progressed very slowly. The performance of the stakes in the tests has so far been equivalent or even better than some CCA preservatives and creosote....
J Jermer, Ö Bergman, T Nilsson


Short term preconditioning of preservative-treated wood in soil contact in relation to performance in field trials
2000 - IRG/WP 00-20185
The effect of pre-exposure to primary colonising micro-organisms on preservative-treated wood, prior to a basidiomycete decay test, was determined by preconditioning in two soil types. Scots pine EN 113 blocks treated with 3 model systems (a triazole, a copper quaternary compound and a copper boron triazole) were leached according to EN 84 and subjected to 6 weeks and 8 weeks burial in either John...
S Molnar, D J Dickinson


Elimination of alternative explanations for the effect of iron on treated wood
1993 - IRG/WP 93-30006
Amounts of iron which had previously been found in stakes removed from ground contact reduced decay of untreated wood by four brown-rot fungi. This suggested that the effect of iron may be on the preservative. Analysis of the leachates from CCA- and ACA-treated wood blocks first exposed to rusting iron, then to a brown-rot fungus, showed that the increased decay found in the laboratory for wood ex...
P I Morris, J K Ingram, D L Gent


Efficacy of some extractives from Pinus heartwood for protection of Pinus radiata sapwood against biodeterioration. Part 1: Fungal decay
1995 - IRG/WP 95-30072
Chemical compounds thought to contribute to the natural durability of heartwood of Pinus spp. were either chemically synthesised in the laboratory or extracted from the heartwood of Pinus elliottii or Pinus caribaea. These compounds included the stilbenes, pinosylvin and its mono- and di-methyl ethers, and the flavonoids, pinobanksin and pinocembrin. Small blocks of Pinus radiata sapwood were impr...
M J Kennedy, J A Drysdale, J Brown


A short note on fungal decay in K33-treated poles
1982 - IRG/WP 1169
Soft rot cavities and erosion of the lumen have been found in K33-treated Pinus sylvestris poles from the years 1956-66 by microscopic studies. Poor treatment quality has been proved for some of these poles. The microscopy showed an unusual pattern of attack, and pre-treatment decay is suspected but not yet proved. Sounding the poles and using the Pilodyn indicated decay, but poking did not. Quant...
H Friis-Hansen


Localization of oxalate decarboxylase in the brown-rot fungus Postia placenta
1996 - IRG/WP 96-10161
Oxalate decarboxylase, the enzyme that breaks oxalic acid down into formic acid and carbon dioxide, was recently detected in mycelial extracts of the brown-rot fungus Postia placenta. Differential centrifugation was used to demonstrate that the enzyme is loosely associated with the hyphal surface. Enzyme activity can be removed by washing the hyphae with a low pH buffer. Only low levels of activit...
J A Micales


The potential of 2-deoxy-D-glucose as an active ingredient in wood preservation
1999 - IRG/WP 99-30205
2-deoxy-D-glucose (2-DOG) is a potential active ingredient against wood decaying fungi. When dissolved in water, it can be used in pressure treatment of wood. Thereby the wood is protected from attack by wood decaying fungi. A concentration of 1.5% (mean retention 9.7 kg/cubic meter sap-wood) is adequate for brown rot fungi, and 3% (mean retention 19.5 kg/cubic meter sapwood) will also provide protection against white rot fungi. 2-DOG is easily soluble in water, and is therefore easily leached from the wood upon completion of the preservation process. Different types of fixa-tion methods have been tried and evaluated. It is possible to produce 2-DOG by the hydrolysis of chitin, a constituent of the exterior skeleton of shellfish and insects. There is a potential for exploita-tion of this waste product provided by the crab and shrimp industry. The yield of 1 kg of fresh shrimp is 75 g of 2-DOG.
O V Frederiksen, A P Koch


Evaluation of the decay caused by Chaetomium globosum Kunze, in the course of time
1987 - IRG/WP 2288
The main research done on soft rot has been directed to determining, by microscopic study, the different stages of penetration into the wood of the fungi that cause it. On the basis of the information furnished by this research, in this work we have tried to quantify its effect, by evaluating the weight loss caused by Chaetomium globosum Kunce in wood of Pinus sylvestris L. and Fagus sylvatica L. ...
M T De Troya, A M Navarrete


Conditions for basidiospore production in the brown rot fungus Gloeophyllum separium in axenic culture
1984 - IRG/WP 1232
Attempts to control and optimize the production of hymenial structures and basidiospore production in Gloeophyllum sepiarium in axenic culture resulted in the proposal of the following conditions as being suitable. The dikaryotic mycelia originally isolated from basidiocarps could consistently be induced to produce hymenial structures and pure basidiospore collects if illuminated by near ultraviol...
J Bjurman


The use of C CP/MAS NMR in the chemical identification of decayed and undecayed, tropical timber species
1984 - IRG/WP 1224
13C CP/MAS NMR was found to be an extremely powerful tool for elucidating the chemical composition of Eucalyptus maculata, Pinus elliottii and Alstonia scholaris. The differences in lignin composition were different for each timber and discussed in relation to decay caused by soft-rot and white rot fungi. In particular the presence of syringyl and guaiacyl lignin types are discussed.
L E Leightley


A new approach to the maintenance of wooden railway sleepers. (Second report)
1988 - IRG/WP 3492
The microenvironment and micro-ecology of wooden railway sleepers was investigated to assess their condition to determine the necessary treatment, repair and replacement criteria. In this report the efficacy of the secondary preservative treatment with solid boron rods is discussed and the development of an in-situ, nondestructive test method based on the creation and assessment of structural dyna...
W Beauford, P I Morris, A M Brown, D J Dickinson


IRG/COIPM INTERNATIONAL MARINE TEST - to determine the effect of timber substrate on the effectiveness of water-borne salt preservatives in sea-water: Final report
1987 - IRG/WP 4133
Three timbers chosen as reference species were treated with 3, 6 and 10% solutions of CCA and CCB preservatives and exposed for up to 93 months at 8 tropical and temperate marine sites. Eleven local species treated in the same way were exposed at 4 of the 8 sites. There was no apparent difference in performance between CCA and CCB treated specimens. The severest test site was Panama Canal but mari...
R A Eaton


A summary of tests and practical experiences with the Pilodyn wood testing instrument
1980 - IRG/WP 282
This paper presents a summary of the reports, tests and practical experiences with the Pilodyn wood tester not only, however, concerning poles but also in other fields such as standing trees, sawn timber etc. The principle of the Pilodyn is a spring-loaded pin which is fired into the object and the depth to which the pin penetrates is correlated to physical and mechanical properties of the object....
H Friis-Hansen


Effects of some essential oils on wood destroying organisms
1993 - IRG/WP 93-10047
Three wood destroying fungi: Botryodiplodia theobromae Pat. (stain), Gloeophyllum sepiarium (brown rot), and Pycnoporus sanguineus (white rot) were exposed to six plant essential oils: the peppermint, kaffir lime or leech lime, lavender, tarragon, holy basil, and the eucalyptus. The peppermint oil showed most effective to inhibit fungal growth, while eucalypus oil was the least effective. The othe...
K Atisongkroh, C Anantachoke, P Lekuthai, S Pensook, T Kittirattrakarn


Basidiosporogenesis by brown-rot basidiomycetes in vitro
1995 - IRG/WP 95-10126
Basidiospores of wood-rotting basidiomycetes are a primary sourse of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia car...
S C Croan


Final draft of a monographic card for Pole Fungus A, L Harmsen, Rigidoporus vitreus (Fr.) Donk according to the 'Model Questionnaire for preparation of monographic cards for wood-destroying fungi'
1978 - IRG/WP 178
L Harmsen


Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner


Oxalic acid production of fifteen brown-rot fungi in copper citrate- treated southern yellow pine
2001 - IRG/WP 01-10388
Non-arsenical copper-based wood preservatives have grown in number since the 1980's as a response to environmental concerns posed by arsenicals. Interest in copper tolerant decay fungi has increased accordingly. Oxalic acid (OA) production by brown-rot fungi has been proposed as one mechanism of copper tolerance. Fifteen brown-rot fungi representing the genera Postia, Wolfiporia, Serpula,...
F Green III, C A Clausen


Ultrastructural observations on wood-degrading erosion bacteria
1986 - IRG/WP 1283
G F Daniel, T Nilsson


Differential behaviour of wood rotters at water repellent Rubberwood
2000 - IRG/WP 00-10386
Rubberwood samples treated with different concentration of aqueous solution of chromic acid has induced significant increase in water repellency and attains dimensional stability. Though water repellency and dimensional stability was achieved by treatment, the fungal invasion could not be completely controlled. Differential level of decay of brown and white rot in wood treated with water repellent...
H C Nagaveni, S S Chauhan, K S Rao


Micromorphology of the decay caused by Chondrostereum purpureum (Pers.:Fr.) Pouzar and Flammulina velutipes (Curt.:Fr.) Singer
1988 - IRG/WP 1358
Two basidiomycetes, Chondrostereum purpureum and Flammulina velutipes, form typical soft rot cavities in hardwoods and softwoods. Cavity formation is prececed by T-branching or bending of hyphae penetrating the wood cell walls. The two fungi also cause erosion of the wood cell walls....
T Nilsson, G F Daniel


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