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Mycoparasitism by some white rot fungi on blue stain fungi in culture
1986 - IRG/WP 1304
When studying biological control of blue stain an interesting phenomenon of parasitism by some white rot fungi on blue stain fungi has been encountered. The majority of the 22 tested white rot fungi act parasitically upon blue stain fungi. The most interesting species among them is Bjerkandera adusta (Willd. ex Fr.) Karst., causing almost complete or complete decoloration of the hyphae of the blue...
R Benko, B Henningsson


Characterization of wood decay enzymes by MALDI-MS for post-translational modification and gene identification
2002 - IRG/WP 02-10442
The recent sequencing of the Phanerochaete chrysosporium genome presents many opportunities, including the possibility of rapidly correlating specific wood decay proteins of the fungus with the corresponding gene sequences. Here we compare mass fragments of trypsin digests, determined by MALDI-MS (Matrix Assisted Laser Desorption Ionization-Mass Spectrometry), with predicted mass fragments derive...
T H de Koker, P J Kersten


Water transfer in wood in relation to fungal attack in building - Effect of condensation and diffusion
1992 - IRG/WP 92-1543
Wood is continuously exchanging moisture with the surrounding atmosphere, the temperature and relative humidity are important factors. Wood moisture in this case is not enough to provoque decay by fungi. However, when, during the night, condensation appears cyclically, wood absorbs water and moisture content may be sufficiently high to allow development if drying does not take place. The water tra...
D Dirol, J-M Vergnaud


Assessment of the biocontrol potential of a Trichoderma viride isolate in a field trial
1998 - IRG/WP 98-10252
A field trial has been set up near Dundee, to assess the biological control potential of a Trichoderma viride isolate T60. This isolate has been shown in laboratory tests to be particularly effective in protecting wood against certain basidiomycete decay fungi. Wood was treated with T60 spores using vacuum-pressure impregnation in a pilot preservation plant. Scots pine and Sitka spruce stakes wer...
H F Brown, A Bruce


Why don't more people work with Serpula lacrymans nowadays? A discussion of some of the different approaches to experimenting with this unique fungus
1989 - IRG/WP 1383
Remarkably few laboratories are currently investigating this well-known fungus. Not only has it failed to gain acceptance as a standard test organism (for wood preservative evaluation) in many countries, but is also sometimes excluded from pure research studies when it ought to be an obvious first choice as a representative basidiomycete. This paper discusses some different approaches that have be...
J D Thornton


Leaching tests on CCA-treated wood using inorganic salt solutions
1984 - IRG/WP 3310
Pinus radiata sapwood treated to approximately 10 kg/m³ with Tanalith NCA or Tanalith C-type CCA preservatives was ground to a fine powder and samples were then leached with solutions of calcium chloride, calcium nitrate, magnesium nitrate, or a mixture of potassium di-hydrogen orthophosphate and di-potassium hydrogen orthophosphate, at 0.03M-1.00M solution concentrations. Leached wood samples we...
D V Plackett


A non-destructive testing technique for wood poles
1987 - IRG/WP 2293
A method has been developed to evaluate the stiffness and condition of a wood pole based on structural dynamics. This paper describes the results of tests performed on nearly 200 wood utility poles across Western Canada. A high correlation exists between the dynamic non-destructive test and direct mechanical tests. The need for an accurate non-destructive testing technique is demonstrated by the f...
W M Murphy, D E Franklin, R A Palylyk


Wood cement composites using spent CCA treated wood
1999 - IRG/WP 99-50126
The feasibility of using spent or out-of-service CCA treated wood as a component of wood/cement composites was evaluated. Cold pressed wood particle cement boards were made using CCA treated particles from a red pine (Pinus resinosa Ait.) pole removed from service and from untreated red pine wood from a new pole. Boards were manufactured using a range of wood to cement ratios and water to cement r...
Chen Huang, P A Cooper


A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl


Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth


The control and pretreatment decay in air seasoning Scots and Corsican pine poles in England
1990 - IRG/WP 1451
Previous work clearly showed that air-seasoned, British grown Scots and Corsican pine poles are subject to serious pretreatment decay. In order to control these infections a range of pretreatments are currently being investigated. One series of treatments is designed not only to control decay but also allow the development of mould and stain fungi in order to achieve the desired increases in poros...
D J Dickinson, A R Zahora, A P Dodson


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


Resistance of board materials against fungal decay: A comparative experiment on Kolle flask tests method and direct test method
1985 - IRG/WP 2243
Two sets of plywood (Aucoumea klaineana Pierre) 7 and 11 ply, treated by two preservatives, and untreated, were tested by two methods: 1. Kolle flask test method on malt-agar substrate according to the French standard NF B 51.295; 2. A direct test method in non sterile conditions according to DocNo: IRG/WP/2214. The exposure time far both methods was 12 weeks. After exposure, the degree of attack ...
G R Y Déon, L N Trong


Soft rot in heartwood of preservative-treated pole stubs of Eucalyptus cypellocarpa L. Johnson
1983 - IRG/WP 1204
Pole stubs of Eucalyptus cypellocarpa L. Johnson, treated in the sapwood with certain waterborne preservatives and exposed for 21 to 23 years in a dry tropical region were severely soft-rotted in the heartwood, as well as the sapwood. They broke with a brash fracture when stress tested (loaded as a cantilever) in the field. The severe soft rot in the heartwood was not detected by the knife test or...
J E Barnacle, G C Johnson, M A Tighe


Evaluation of fungi isolated from the galleries of the striped ambrosia beetle Xyloterus lineatus (Ol.) (Col., Scolytidae)
1995 - IRG/WP 95-10092
Fungi from the galleries of the striped ambrosia beetle Xyloterus lineatus (Trypodendron lineatum) (Ol.) found in spruce logs were studied. The following fungi were isolated and identified: Trichoderma harzianum Rifai, the yeast Pichia anomale Hansen (Kurzman), the blue-stain fungus Ceratocystis piceae (graphium) (Münch) Bakshi and a fungus closely similar to Ceratocystis araucariae (anamorph). T...
G Babuder,F Pohleven


A comparison analysis of eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray
1991 - IRG/WP 2362
Investigations were previously carried out to compare eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray (some used in various countries as standard test strains): FPRL 12C (England), FPRL 12E (Germany), Warsaw III (Poland), HFP 7802 (Japan), DFP 16508, 16509, 16521 and 16522 (Australia). Studies included growth rate and dry mass of mycelium, decay capacity, reduction of compression stre...
J Wazny, J D Thornton


Factors affecting the resistance of fibre building boards to fungal attack
1975 - IRG/WP 252
Fungal decay is initiated at lower moisture contents in standard and tempered hardboards (18%) than in pine sapwood (26%). In contrast, in a saturated atmosphere, the equilibrium moisture contents of standard hardboard (14%) and of tempered hardboard (12.5%) are much lower than the moisture content permitting decay initiation whilst the equilibrium moisture content of pine sapwood (25.1%) approach...
C Grant, J G Savory


Effects of CCA treatment on settlement and growth of barnacles under field conditions
1998 - IRG/WP 98-50116
As part of a field experiment at seven European marine sites evaluating the effects of CCA treatments on non-target marine organisms, panels treated to nominal loadings of 12, 24 and 48 kg/m3 CCA were exposed at marine sites around the coast of Europe. Within two weeks of exposure, a heavy settlement of barnacles took place on the panels exposed intertidally in France. The intensity of settlement ...
S M Cragg, C Brown, R M Albuquerque, R A Eaton, P Goulletquer


Evaluation of decay fungi in Norwegian buildings
2005 - IRG/WP 05-10562
During a period of 2 years and 3 months (1 January 2001 - 20 March 2003) Mycoteam had 3161 consultations in buildings in southern Norway, 1428 revealing damage from decay fungi. One consultation often revealed several occurrences of fungi, and the total number of occurrences of decay fungi was 3434. Thirty-five different species/genera/groups of decay fungi were recorded. During this period brown ...
G Alfredsen, H Solheim, K Mohn Jenssen


Sawmill Evaluation of a Bioprotectant against Moulds, Stain and Decay on Green Lumber
2003 - IRG/WP 03-10460
Moulds, stain and decay cause serious problems on wood utilization. Though a growing volume of wood is kiln-dried, the market for green exports remains significant, and environment-friendly wood protection is required to replace traditional chemicals. As a solution to this problem, Forintek Canada Corp. developed a bioprotectant for protecting logs and green lumber from moulds, stain and decay, wh...
Dian-Qing Yang, M Gignac, M-C Bisson


Performance of above ground decay test units treated with a substituted isothiazolone
1989 - IRG/WP 3503
RH 287, a substituted isothiazolone, was evaluated for efficacy against decay fungi in an above ground test in Mississippi. After 45 months of exposure, all test units that were pressure treated with this biocide at levels of 0.026 pcf and higher have no deterioration. In addition, the test units that were dip-treated with a solution concentration of 0.5% also have no deterioration. Compared to pe...
D D Nicholas, J A Wilson, D E Greenley


Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley


Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson


Evaluation of extractives from African Padauk (Pterocarpus soyauxii Taub.) for protection of non decay resistant species
2002 - IRG/WP 02-10419
The development of a wood preservative made up of natural extracts is a potentially attractive prospect for the wood preservation industry. In this study, organic solvents were used to remove extractives from African padauk wood samples. The solvent was removed by rotoevaporation, and the total content of the viscous extracts determined to be 54.4mg/ml. The toxicity of various extracts concentrat...
P Nzokou, D P Kamdem


Acoustic technique for assessing decay in preservative treated wood
1998 - IRG/WP 98-20138
This study investigated the suitability of vibration techniques to assess the performance of wood preservatives in ground contact. Small stakes (10 x 5 x 100 mm3) of treated and untreated Scots pine sapwood were exposed to decay in lab-scale terrestrial ecosystems. Tests were conducted using three different soils including a garden compost soil, and soils obtained from a test field and a conifer f...
L Machek, M-L Edlund, R Sierra-Alvarez, H Millitz


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