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The invasion channels of damage fungi in bamboo lumber
2010 - IRG/WP 10-10712
The micro-agents on bamboo lumber damage are mould, stain, and decay fungi. This research is about invasion channels of bamboo lumber damage fungi by scanning electron microscope (SEM). The results showed that the conidia of mould were only accumulated on the surface of bamboo and no hyphae detected in bamboo vascular bundle, and hyphae of stain penetrated into bamboo and grew in metaxylem vessel...
Xingxia Ma, Mingliang Jiang, Daochun Qin


Fungal Attack on Lignin and Cellulose: Elucidation of Brown- and White-Rot Mechanisms Comparing Biomimetic and In-Vivo Degradation Patterns
2010 - IRG/WP 10-10714
This paper examines research and hypotheses that have been developed over several years on wood degradation mechanisms. This information is combined with new data and analyses to explain why wood decay patterns caused by brown-rot fungi and specific types of white-rot fungi are different. New data, including work with both biomimetic studies on low molecular weight compounds, degradative enzymes, ...
V Arantes, B Goodell, A M F Milagres, Yuhui Qian, T Filley, J Jellison, S Kelley


Assessment of antagonism between lignicolous microorganisms: Research on possible use to preserve wood poles
2010 - IRG/WP 10-10718
Biological environments contain a certain number of microbial populations which, within a given ecological niche, display various relations ranging from symbiosis to parasitism. Researchers have been interested in these types of relations for around fifty years, especially in one very particular type of relationship: the antagonism exerted between individuals of the same microbial population. Toda...
J-F Labrecque, A Zaremski, L Gastonguay, Y Prin


A genomic sequencing approach to study wood decay and copper tolerance in the brown rot fungus, Antrodia radiculosa
2010 - IRG/WP 10-10720
We used Illumina paired-end short read sequencing (76 nt, 300 bp insert size) to produce a de novo assembly of the genome of Antrodia radiculosa, a copper-tolerant brown rot fungus that is capable of aggressive wood decay. Quality analysis of the base calls in the dataset (8.95 Gb) showed that the majority of the nucleotide sequence was of the highest quality with 5% in the lowest quality group. ...
J D Tang, T Sonstegard, S Burgess, S V Diehl


Qualitative - Quantitative Analysis of Wood-Inhabiting Fungi in External Wooden Structures of the Latvian Cultural Heritage
2010 - IRG/WP 10-10728
The frequency and diversity of wood inhabiting fungi in the exterior wood were established in the Araishi lake fortress, the Lielvarde wooden castle, the Latvian Ethnographic Open-Air Museum, Riga, and twelve wooden heritage objects in the Eastern part of Latvia (Latgale). The inspected wooden structures of the external woodwork included windows, stairs, walls, floorings, roofs, fences, and benche...
I Irbe, M Karadelev, B Andersons


Early detection of colonisation by blue stain and mould on modified wood using PCR technique
2010 - IRG/WP 10-10730
The aim of this research was the early detection of fungal attack on the surfaces of modified and unmodified wood after natural weathering. The investigation was carried out by molecular techniques and the development and establishment of genus/species specific PCR for important moulds and sapstain fungi was part of this work. After adaption of the method, a monitoring of the succession of Ascomyc...
A Steitz, B Schmöllerl, N Pfabigan, S Bollmus, G Grüll


Glutathione transferases as functional biomarkers of wood degradation?
2010 - IRG/WP 10-10735
The recent release of several fungal genome sequences allows the report of an overview of fungal glutathione transferases (GSTs) focused on their function in the wood-degrading basidiomycete Phanerochaete chrysosporium. A genomic and phylogenetic analysis of GST classes in various sequenced fungi –zygomycetes, ascomycetes and basidiomycetes- revealed some specificity in GSTs distribution, in com...
E Gelhaye, M Morel


Natural durability of Mulberry wood from Iran
2010 - IRG/WP 10-10737
Mulberry wood (Morus alba L.) is traditionally used in Iran to manufacture music instruments such as tar and setar. But little is know and contradictory statements are found on the natural durability of Morus alba. Moreover, in many cases, Iranian instrument makers leave freshly cut Mulberry timber into water (cold or hot) for a time period depending on the craftsman habit, without any real knowle...
A Se Golpayegani, M-F Thevenon, J Gril, K Pourtahmasi


Measurements of rot fungal activity as a function of moisture content by isothermal calorimetry
2010 - IRG/WP 10-20428
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. I...
L Wadsö, A Pilgård, G Alfredsen


Detection of Wood Destroying Fungi Using DNA Microarray Technology
2010 - IRG/WP 10-20435
Wood decay fungi of the phylum Basidiomycota cause serious damage to wooden constructions and building elements. The elimination and the appraisement of fungal decay require an assured species identification. Conventional fungal diagnostics are mainly based on morphological characteristics by macro- and microscopy analysis. For some years, standardised and highly sensitive molecular methods focuss...
K Jacobs, N Rangno, W Scheiding, B Weiss, D Müller, C Hiller, W Brabetz


Profiling fungal community in wood decay ecosystem by Denaturing High-Performance Liquid Chromatography
2010 - IRG/WP 10-20443
A DNA-based fingerprinting technique, Denaturing High Performance Liquid Chromatography (DHPLC) was developed to profile fungal communities colonizing indoor timber. Molecular fungal diversity was assessed using amplification based on the Internal Transcribed Spacer (ITS1) rDNA of 74 wood samples collected from infected buildings in France. Due to its high sensitivity, the PCR-DHPLC technique was ...
S Maurice, G Le Floch, M Le Bras-Quéré, J P Rioult, G Barbier


DNA-based tools for rapidly detecting, quantifying and monitoring ophiostomatoid fungi on beetles, in trees and wood products
2010 - IRG/WP 10-20450
Approximately half of the trees harvested for commercial purposes are lost because of native or introduced insects or insect-vectored microorganisms. Ophiostomatoid fungi, which are well adapted to dissemination by insects, include ~140 species of saprobes and pathogens. They are present worldwide, have high economical impact and many are subject to quarantine regulation. Thus, it is necessary to ...
L Khadempour, Young Woon Lim, S Massoumi Alamouti, C Breuil


Effects of Cunninghamia Lanceolata Heartwood Extracts on the Growth of Wood Decay Fungi
2010 - IRG/WP 10-30527
China-fir (Cunninghamia lanceolata) wood is well known for its good durability. This species is native to China and has been extensive planted there. Mainly, extractives are responsible for the wood resistance to decay fungi. In this paper, the extractives of China-fir heartwood were studied for their effects on various wood decay fungi. Sequential extraction of heartwood in hexane, ethyl acet...
Shujun Li, Jing Wang, Jian Li


Evaluation of ACQ-D treated Chinese fir and Mongolian Scots pine with different post-treatments after 20 months of exposure
2010 - IRG/WP 10-30530
The performance of alkaline copper quat-type D (ACQ-D) treated Chinese fir (Cunninghamia lanceolata Hook.) and Mongolian Scots pine (Pinus sylvestris Linn. var. mongolica Litv.) stakes after 20 months exposure in Chengdu and Guangzhou of southern China were evaluated according to AWPA standard E07-07. The ACQ-D treatments used two concentration levels (0.5 and 1.0%) and four different post-treatme...
Lili Yu, Jinzhen Cao, Wei Gao, Haitao Su


Antifungal Essential Oil Metabolites
2010 - IRG/WP 10-30531
New environmentally-friendly wood protection systems based on “green” technologies are needed to inhibit wood-inhabiting mold and decay fungi. Utilizing bioactive essential oils from select herbaceous plants is one promising approach, but the concentrations of bioactive compounds are somewhat variable even in the highest (therapeutic) grade essential oils. Purified primary metabolites from fou...
C A Clausen, B M Woodward, V W Yang


Degradation of Carbon-Based Preservatives by Black-stain Fungi
2010 - IRG/WP 10-30533
Some black-stain fungi are known to exhibit resistance to carbon-based preservatives. However, the cause of this resistance is unclear. The fungi may have mechanisms to tolerate these chemicals, or they may be able to degrade and detoxify them. Fungi capable of degrading carbon-based preservatives are of interest for possible bioremediation of carbon-based preservative-treated wood at the end of i...
R Stirling, P I Morris


Natural compounds: A review of their use for wood protection
2010 - IRG/WP 10-30545
A lot of research in the field of wood protection has focused on natural compounds but very few of these have been implemented by industry. This review is an attempt to bring together information from selected area of work: - A brief review of the work done on natural products as organic biocides for wood protection. - Table containing information on natural products, their use as organic biocid...
T Singh, A P Singh


Wood protection by means of electro osmotic pulsing technology (PLEOT)
2010 - IRG/WP 10-40505
Wood protection is mainly based on chemical protection of wood. The disposal of wood preservative treated material causes restrictions in its later use or recirculation into the eco-cycle. A new protective system, electro-osmotic pulsing technology on wood, called PLEOT, is tested in a fungi test and in soil contact. Mass loss and moisture content of Scots pine sapwood samples was calculated after...
A Treu, E Larnøy


Testing of decay resistance of sapwood and heartwood of thermally modified Scots pine and Norway spruce
2010 - IRG/WP 10-40523
Thermal modification at elevated temperatures changes the chemical, biological and physical properties of wood. One of the main targets of thermal modification is to increase the biological durability and decay resistance of wood. Although the effects of thermal modification on wood have been widely studied, the significance of sapwood and heartwood on the resistance of thermally modified wood has...
H Viitanen, S Metsä-Kortelainen


Bioresistance of Poplar Wood Compressed by Combined-Hydro-Thermo-Mechanical Wood Modification (CHTM) Process
2010 - IRG/WP 10-40532
Bioresistance of treated Poplar wood by CHTM process (Combined-Hydro-Thermo-Mechanical Wood Modification) was studied in the current research work. Resistance against brown rot fungus Gloeophyllum trabeum as well as the soft rot decay was the main concern of this work. Poplar wood blocks were hydrothermally treated at temperatures of 120,150 and 180°C for holding time of 0, 30 and 90 min. afterwa...
L Khademi-Bami, B Mohebby


Fungal biodegradation of CCA-treated wood wastes
2011 - IRG/WP 10-50276
Fungal biodegradation could be employed as a pretreatment method to alleviate problems caused by landfill disposal of CCA-treated wood wastes (e.g. a shortage of landfill space and a release of leacheate). It could be used to decrease the volume of waste and to remove metals from waste simultaneously. We have screened hundreds of decay fungi, including fungi isolated from CCA-treated wood in servi...
Gyu-Hyeok Kim, Yong-Seok Choi, Jae-Jin Kim


Gene expression analysis of a copper-tolerant brown rot fungus on MCQ-treated wood
2011 - IRG/WP 11-10748
Most brown rot fungi are copper-tolerant, which makes them difficult to control with copper-based wood preservatives like MCQ. To better understand what biological processes are regulated, we used our model species, Antrodia radiculosa, to examine expression of genes on MCQ-treated wood. Our hypothesis was genes that decreased copper bioavailability would be up-regulated early, when wood showed ...
J D Tang, A Perkins, S V Diehl


Measuring fungal cellulolytic enzyme activity in degrading wood
2011 - IRG/WP 11-10753
An enzyme assay which measures enzyme activity directly from wood will assist in the fundamental understanding of the enzyme components of the decay mechanism of rot fungi and any changes in the presence of wood protectants. At present, to measure fungal carbohydrate degrading enzyme activity in the presence of wood, two methods are used: either 1) the enzyme is measured in the growth media contai...
S M Duncan, J S Schilling


Quantification of four dark colored mould fungi by real time PCR
2011 - IRG/WP 11-10754
Coated wooden claddings in building facades are widely used in the Scandinavian countries, and are often preferred to other materials. Wood is facing increasing competition from other materials that are less labor intensive at the construction site and materials with less demand for maintenance thru service life, and makes further development of wooden claddings essential. Growth of discoloring mo...
E Larnøy, L Ross Gobakken, A M Hietala


Characterisation and evaluation of various chitosan oligomers, and decay resistance
2011 - IRG/WP 11-30562
Chitosan, a polymer of D-glucosamine, is known for its antimicrobial activity. However, the physicochemical properties of chitosan depend upon three principal factors, i.e. source of raw material, molecular weight and degree of deacetylation. Here, we report synthesis and characterization of chitosan oligomers prepared by deaminative depolymerization of chitosan (s) obtained from Sigma Aldrich and...
I Hussain, C Chittenden, T Singh


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