IRG Documents Database and Compendium


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Effects of the specimen position on fungal colonisation and wood decay by en 113 test fungi
1998 - IRG/WP 98-20136
For testing wood preservatives according to EN 113 it is common practice to plant the test blocks on neutral supports in order to prevent (1) a diffusion of chemicals into the agar medium and (2) an excessive moistening of the specimens. The procedure was employed in EN 350-1 for testing the natural durability of solid wood. It turned out to be of problematic nature because of the individual requ...
G Kleist, M-T Lenz, R-D Peek


Micromorphology of oak wood degraded by brown rot fungus Coniophora puteana
2000 - IRG/WP 00-10356
To characterize the degradation of hardwood by brown rot fungus, Coniophora puteana was incubated on the wood blocks of Quercus accutissima. For comparison, the same fungus was also incubated on the pine wood blocks of Pinus densiflora. In softwood, the shape of the wood cells and thickness of the cell appeared unchanged even the loss of birefringence in the affected areas. In contrast, oak wood d...
Yoon Soo Kim, Seung-Gon Wi, Kwang-Ho Lee


Biodetioration and strength reductions in preservative treated aspen waferboard
1983 - IRG/WP 2195
Experimental aspen waferboards, bonded with liquid or powdered phenol formaldehyde resins and treated by various methods with a wide selection of preservatives, were tested for fungal resistance in accelerated laboratory trials. Mold growth on the surface as well as weight and strength losses due to the actions of decay fungi were determined. Testing of board strength after decay in high and moder...
E L Schmidt, H J Hall, R O Gertjejansen, R C De Groot


Investigations on testing with soft rot fungi by the vermiculite-burial method
1972 - IRG/WP 215
With the object of developing a test method to determine the effectiveness of wood preservatives against soft rot attack, tests have been carried out with pure fungal cultures using vermiculite as the substrate for untreated and preservative impregnated wood blocks. In these tests the suitability of common commercial, simple disposable beakers was proved successful as a substitute for the glass bo...
W Kerner-Gang, M Gersonde


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce


Chemostimulatory and chemotropic responses by fungi to preserved and unpreserved wood
1981 - IRG/WP 1134
During experiments to determine the presence of biotic connections between soil and wood at this laboratory, marked chemotropic responses to wood were demonstrated by some wood destroying micro-organism in agar culture. These were shown at some distance from bait, up to 35 mm away, and on some occasions were unaffected by the presence of toxic materials. This paper provides preliminary data on the...
G Mowe, B King


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


Laboratory trials of biological control agents against subterranean termites
1991 - IRG/WP 1475
To screen for potential termite control by microorganisms, 17 mold fungi included 3 pathogenic fungi and 7 basidiomycetes, were used to challenge the test termite species, Coptotermes formosanus and Reticulitermes speratus. Test fungi were allowed grow and completely in Petri dishes. To screen for potential termite control by microorganisms, 17 mold fungi included 3 pathogenic fungi and 7 basidiom...
K Suzuki


Northern oak wood and its damages in Iran
1988 - IRG/WP 1377
The Northern oak species of Iran designated Quercus castaneaefolia C.A.M. is distributed widely in caspian littoral. In natural conditions (healthy) has favourable quality and it is used mostly in rural construtions, sleepers, parquets, cross arms for electric and communication poles, it is used also indoor and window construction, benches and finally veneer. This wood in the case of sleepers and ...
D Parsapajouh, P Niloufari


Variation in biological performance of CCA caised by preservative application method
1996 - IRG/WP 96-40072
A series of laboratory studies to investigate the influence of treatment application method on CCA performance in Cosican pine has been completed. Biological decay tests, such as serial exposures, were used to induce decay in wood at preservative retentions of up to 10 kg/m³ CCA salts. Significant differences in performance of the preservative against either brown, white or soft rot decay fungi w...
P R Newman, R J Murphy


Physiological properties of fungal test strains according to the European Standard EN 113
1986 - IRG/WP 2258
For the discussion of the European standard EN 113 the EMPA's procedure of culturing the test fungi and the corresponding virulence of the test fungi as well as the wood moisture content at the end of the test are shown. It is mainly shown that within the standard the choice of the solvent may not be left at the test lab if reproducible results shall be obtained. The different solvents in...
E Graf, B Zgraggen, P Manser


Isolation and identification of the fungal flora in treated wood. Revised technique
1977 - IRG/WP 159
At the 8th Annual Meeting in Wildhaus a paper was presented for discussion on the isolation of fungi from treated wood.·Since then work of this nature has been undertaken at Imperial College and as a result a revision of that document has been made and is presented here. The main alterations are: 1) To streamline the isolation procedure 2) Modification of the benomyl agar 3) The inclusion of a st...
C P Clubbe, J F Levy


Influence of the decay of spruce chips by the selected fungi on their chemical structure and the pulp properties
2004 - IRG/WP 04-10510
The paper focuses on changes in the weight and in the molecular structure of spruce chips submitted to long-term storing as well as to medium- and long-term model rotting degradation caused by some chosen fungi identified at storing processes on the pile. For the model decay of chips under laboratory conditions during 3 or 6 weeks the white-rot fungi: Phanerochaete chrysosporium, Heterobasidion an...
R Solár, L Reinprecht, A Geffert, F Kacík


Evaluation of tropolone as a wood preservative : activity and mode of action
2002 - IRG/WP 02-30282
The fungicidal activity of 2-hydroxy cyclohepta-2,4,6-trienone (tropolone) analogue of b-thujaplicin a natural product responsible for the durability of heart wood of several cupressaceous trees was investigated in vitro on growth of white and brown rot fungi. Results obtained show that tropolone, easily prepared from commercially available products, possesses strong fungicidal activity similar ...
P Gérardin, M Baya, N Delbarre, P N Diouf, D Perrin, P Soulounganga, E Gelhaye, J P Jacquot, C Rapin


The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell


A field test on susceptibility of wood-based board materials to moulds
1989 - IRG/WP 3545
A field test on susceptibility of wood-based board materials to mould growth was carried out under practical, but severe, service-conditions. A total of 19 commercially available composite boards were investigated. It was found that all board materials tested were not completely immune to mould attack. Test results revealed that among the three main categories of wood-based boards involved, partic...
Qiao Wang, B Henningsson


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


Biological resistance of wood-based composites under protected, aboveground conditions
2005 - IRG/WP 05-20312
Five kinds of wood-based composites (density fiberboard=MDF, hardwood plywood=HP, softwood plywood =SP, particleboard=PB and aspen oriented strand board=OSB) were tested for their resistance against subterranean termites and decay under protected aboveground conditions. The exposure method was previously developed for evaluating performance of sill plates (dodai) in Japanese homes. Ten replicates ...
K Tsunoda


Uptake of copper by mycelium of wood decay fungi growing on copper S-substituted thioglycolate containing nutrient media
1998 - IRG/WP 98-10291
Mycelia of Trametes versicolor, Coniophora puteana and Poria monticola were grown on potato dextrose agar (PDA) media, containing various concentrations of copper N,N-dimethyldithiocarbamoylacetate (which may be regarded as a copper S-substituted thioglycolate). The tested copper compound revealed relatively low fungicidal activity. After 13-18 day growing period, we determined concentrations of a...
M Humar, M Petric, F Pohleven, P Kalan


Effect of wrapping on movement of chloropicrin, Vapam, and Vanicide TH in southern pine timbers
1987 - IRG/WP 3411
Important fungi that decay wood protucts were "inoculated" into nonpressure-treated southern pine timbers as vapor-sensing agents to evaluate the effect of wrapping on the movement and persistence of fungitoxic concentrations of chloropicrin, Vapam, and Vanicide TH in the timbers. Fumigant movement and persistence in the timbers was enhanced by wrapping the timbers in polyethylene after fumigation...
T L Highley


A comparison of fungal strains used in the bioassay of wood preservatives
1984 - IRG/WP 2220
Previously published data are presented relating to a number of strains of wood-destroying basidiomycetes (Coniophora puteana, Coriolus versicolor, Gloeophyllum abietinum, Gloeophyllum sepiarium, Gloeophyllum trabeum, Lentinus lepideus, Poria placenta, Fibroporia vaillantii and Serpula lacrymans) commonly used as test fungi in the bioassay of wood preservatives. The data, which has not been statis...
J Wazny, H Greaves


The effect of copper/chrome/arsenic (CCA) treated timber on soil fungi
1981 - IRG/WP 1131
The aim of this investigation was to study the effect of CCA treated wood on the microflora of soil with particular reference to copper tolerant soft rot fungi. The IRG collaborative field experiment was designed to monitor the performance of a range of preservative treated hardwoods with particular reference to soft rot in CCA treated timber (Dickinson 1976) and site 33 of this experiment was use...
R J Murphy, D J Dickinson


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


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