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Glutathione transferases and wood extracts
2015 - IRG/WP 15-10845
Wood rotting fungi have developed an efficient detoxification system allowing them to be well adapted to their way of life, these fungi generating and encountering potential toxic compounds during wood degradation. Belonging to this detoxification network, glutathione transferases are particularly extended in these fungi and their presence could reflect the chemical environments encountered by the...
A Deroy, F Saiag, Z Kebbi-Benkeder, N Touahri, M Morel-Rouhier, F Colin, S Dumarcay, P Gérardin,


Production of reactive oxygen species in the presence of oxidized iron and oxalate under conditions mimicking brown-rot fungal degradation of wood
2016 - IRG/WP 16-10861
The biochemical role of oxalate in the fungal solubilization of iron is well known, but additional information is needed on the role that oxalate plays in the brown-rot decay of lignocellulose. In this study iron sequestration from iron and iron oxide-hydroxides was assessed with regard to the function of a chelator-mediated Fenton (CMF) reaction and the generation of reactive oxygen species (ROS)...
Yuan Zhu, Liangpeng Zhuang, B Goodell, Jinzhen Cao, J Mahaney


High-frequency monitoring of mass loss due to brown rot degradation of modified wood
2016 - IRG/WP 16-10862
Fungi growing in liquid culture undergoes three separate phases in which they i) adapt to the new environment, ii) grow unrestrictedly and exponentially, and iii) are inhibited to increase in number/mass due to lack of nutrients etc. Filamentous fungi have been shown to exhibit similar growth phases in a solid food substrate and have been modelled to grow in this way also in solid wood. In modifie...
R Ringman, A Pilgård, K Richter


Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
Prevention of wood decay by fungi of the phylum Basidiomycota is an important issue of wood protection. The most destructive fungus in buildings in Central Europe is the dry rot fungus Serpula lacrymans, which needs more extensive restauration measures than all other wood decay fungi. Its close relative S. himantioides occurs more frequently in outdoor environment. The assessment and remediation o...
K Jacobs, N Rangno


Superhydrophobic treatment of Norway spruce wood for improvement of its resistance against brown rot and moulds
2016 - IRG/WP 16-40734
Water repellence of wood can be increased and its some other properties influenced by introduction of hydrophobic chemicals into and on cell walls without affecting the wood’s bulk density due to lumen filling. Herein, a simple dipping method to insert octadecyltrichlorosilane (OTS) into cell walls and to deposit the self-assembled hydrophobic monolayers (SAMs) of OTS is reported. It was found o...
A Kumar, P Ryparová, P Hajek, B Kričej, M Pavlič, A S Škapin, M Šernek, J Tywoniak, J Žigon, M Petrič


Agar block durability test of plywood made of neem (Azadiracta indica) leaves treated veneer using two white rot fungi
2017 - IRG/WP 17-40782
This study has been undertaken to investigate the durability of plywood made from non-durable wood (simul, Bombax ceiba) veneer treated by neem (Azadiracta indica, A. Juss.) leaves. The veneers were treated with cold and hot water solution of neem leaves of different concentrations (10%, 5%, 2.5%) and treatment durations (1, 2, 3 days) for cold water and 20, 40 and 60 minutes for hot water. The pl...
K Akhter, M A Hashem, S Akhter


Biodegradation of creosote by white rot fungus Phanerochaete chrysosporium and a strains of Pseudomonas
2017 - IRG/WP 17-50329
The aim of this study was to determine degradation effectiveness of polycyclic aromatic hydrocarbons (PAH) contained in creosote by white rot fungus and bacteria. The following two species of bacteria Pseudomonas putida and Pseudomonas sp. as well as Phanerochaete chrysosporium fungus characterised by the ability to decompose aromatic compounds were selected for experiments. Bacterial strains were...
J Zabielska-Matejuk, A Stangierska, A Kropacz, E Kaczorek


Interactions between wood polyphenols and detoxification enzymes of the white rot Trametes versicolor
2018 - IRG/WP 18-10906
Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases (GSTs) is especially expanded in lignolytic fungi, little is known about their physiological target molecules. In this study, by combining thermal shift assay and affinity crystallography we highlighted inter...
M Schwartz, T Perrot, E Aubert, S Dumarçay, F Favier, P Gérardin, M Morel-Rouhier, G Mulliert, F Saiag, C Didierjean, E Gelhaye


FTIR-ATR monitoring of chemical changes of thermo-chemically modified beech wood degraded by brown-rot fungus
2018 - IRG/WP 18-40823
Wood chemical modification with lactic acid oligomers (OLA) has been reported to confer promising properties for wood outdoors applications. To better understand the lactic acid interaction with wood, chemical changes following biological degradation have been characterized with a battery of tests. Fourier transform infrared (FTIR) spectroscopy has been proved to be a valuable tool for studying fu...
C Grosse, M Noël, M-F Thevenon, P Gérardin


The biodegradation of creosote oil by various species of white rot fungi and bacteria isolated from the contaminated soil
2018 - IRG/WP 18-50334
The aim of the study was to determine biodegradation effectiveness of the selected polycyclic aromatic hydrocarbons (PAHs) contained in creosote oil, by two white rot fungal species (Bjerkandera adusta and Irpex lacteus) and three bacterial species (Pseudomonas putida, Pseudomonas sp. OS4 and Rahnella aquatilis). Bacteria were isolated from the soil contaminated with petroleum hydrocarbons from th...
M Sydow, J Zabielska-Matejuk, E Kaczorek, AStangierska, A Kropacz


Non-stochiometric oxidation and ROS generation promoted by guaiacol lignin structures and lignocelluose surfaces may be a component of brown rot fungal degradation mechanisms
2019 - IRG/WP 19-10937
Model guaiacol compounds representing lignin monomers, as well as DHP-lignin and wood flour of controlled particle size were used to assess iron reduction at the pH of the natural wood cell wall. All compounds functioned as electron donors for ferric iron, with the lignin monomers demonstrating capacity for non-stochiometric reduction of iron with multiple moles of ferric ion reduced per mole of l...
Y Tamarua, M Yoshidaa, L D Eltisb, B Goodell


From fungal detoxification systems to wood durability in neotropical forests
2019 - IRG/WP 19-10946
White-rot fungi are able to mineralize all the wood components due in particular to an efficient detoxification system. We hypothesised that components of this detoxification systems, glutathione transferases, could be used as tools to explore the natural durability of neotropical wood species. Analysis of the interactions between six glutathione transferases of Trametes versicolor and extracts of...
T Perrot, G Salzet, N Amusant, J Duchene, E Gelhaye


Characterization of Pectinases from Brown-rot Fungus Fomitopsis palustris
2019 - IRG/WP 19-10951
Brown-rot fungi occur on softwood used as building materials and cause destructive breakdown of wood structure. Therefore, a more accurate understanding is important from the perspective of wood protection. Previous studies have reported that hyphae of brown-rot fungi go through bordered pits on tracheids when the fungi grow into softwood [Francis W.M.R.Schwarze (2007)], and torus existing in the ...
Y Tanaka, N Konno, T Suzuki, N Habu


Impact of water holding capacity and moisture content of soil substrates on the moisture content of wood in terrestrial microcosms
2019 - IRG/WP 19-20662
Terrestrial microcosms (TMC) are frequently used for testing the durability of wood and wood-based materials as well as the protective effectiveness of wood preservatives. In contrary to experiments in soil ecology sciences, the experimental set-up is usually rather simple. However, for service life prediction of wood exposed in ground, it is of immanent interest to better understand the different...
C Brischke, F L Wegener


Biocidic effect of nano metal fluorides against brown-rot fungi
2019 - IRG/WP 19-30732
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredients in wood preservatives such as copper and boron. The effectiveness of nano metal fluorides was compared to sodium fluoride treated wood samples. Wood specimens treated with homo-dispersed sols of MgF2 and CaF2 were tested against brown-rot...
S M Usmani, K Klutzny, I Stephan, E Kemnitz


Pigment production by the spalting fungus Scytalidium ganodermophthorum and its industry potential
2020 - IRG/WP 20-10957
Scytalidium ganodermophthorum is best known as a pathogen of cultivated mushrooms, with infected cultures turning yellow in color. The fungus is also used in the art form known as ‘spalting’ to produce yellow, green, and purple colors in wood for decorative purposes. Wood colored by fungus in this manner has been traditionally used in the creation of fine art and woodworking since the 16th cen...
R C Van Court, P Vega Gutierrez, S C Robinson


FTIR analysis of wood blocks decayed by brown-rot fungi
2020 - IRG/WP 20-10959
Calibration curves of the relative lignin contents in Cryptomeria japonica decayed by brown-rot fungi were developed with Infrared Spectroscopy and Klason technique. First, wood decay test was conducted using brown-rot fungi (Fomitopsis palusris, F. pinicola, Wolfiporia cocos, Gloeophyllum trabeum, and Neolentinus suffrutescens) and white-rot fungus (Trametes versicolor) was used as a comparison. ...
R Kondo, Y Horikawa, R Kose, M Yoshida


The iron reduction by chemical components of wood blocks decayed by wood rotting fungi
2021 - IRG/WP 21-10979
Brown-rot fungi, a group of wood rotting fungi, is well known to be one of major microorganisms that cause the deterioration of wooden buildings in Japan and have been considered to use chelator-mediated Fenton (CMF) reaction in concert with hydrolytic and redox enzymes for degradation of wood cell wall. CMF can be described as a non-enzymatic degradation system that utilizes hydroxyl radicals pro...
R R Kondo, Y Horikawa, K Ando, B Goodell, M Yoshida


Changes in topochemistry and mechanical properties of Beech (Fagus orientalis L.) by natural fungus infestation
2022 - IRG/WP 22-10986
Beech is one of the major hardwood species in Europe. It is, however, highly susceptible to fungal attack both in the fresh state and during the storage. Understanding the alteration in chemical and mechanical properties of beech wood during the initial degradation state can lead to improved raw material utilization. Therefore, UV-microspectrophotometer (UMSP) and High-Performance Liquid Chromatog...
H Sivrikaya, M Rehbein, F Divos, S Adamopoulos, R Hosseinpourpia


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to dete...
E E Soytürk, S N Kartal


Studies into the effect of soil type and soil layer on the in-ground decay of European beech
2022 - IRG/WP 22-20681
In this study, European beech (Fagus sylvatica L.) specimens were exposed to three different soil types; Podsol (Podzol), Braunerde (Cambisol), and Pararendzina (Regosol), in adapted terrestrial microcosm (TMC) tests according to CEN/TS 15083-2 (2005). Soils were sampled (250 mm deep) from field sites and separated into their constituent layers to deliver three TMC setups; mineral soil layer only ...
B N Marais, S Kovacs, M Jansen, C Brischke


Steam Pre-conditioning Treatment Prior to Acetylation: Impact on Dimensional Stability, Moisture Response Behaviour, and White-Rot Fungal Resistance of Hevea brasiliensis and Mitragyna ciliata Wood
2022 - IRG/WP 22-40930
The effect of steam pre-conditioning treatment on the dimensional stability, moisture response behaviour, and durability against white-rot fungus P. chrysosporium of acetylated Hevea brasiliensis and Mitragyna ciliata wood species were assessed. Defect-free specimens of both species from the top, middle, and base positions were selected, prepared according to ASTM D143-09 & ASTM D-2017 standards a...
E Uchechukwu Opara, J Mayowa Owoyemi, J Adeola Fuwape


Evaluation of Decay Resistance for the Larch Wood Heat-treated with Superheated Steam
2022 - IRG/WP 22-40956
Heat-treatment of wood is a process that involves applying heat ranging 160–260°C to improve its physico-mechanical properties and resistance against wood rot fungi. The level of the changes in the wood properties by heat-treatment differs depending on the temperature and duration of heat-treatment, as well as the types of heat transfer media used for heat-treatment. The heat-treatment on wood ...
Y Park, S-M Yoon, H Kim, W-J Hwang


A novel cellulose-binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11019
Wood-rotting basidiomycetes are the major organisms decomposing wood in nature. They are classified into two groups based on their decay modes; white-rot fungi and brown-rot fungi. White-rot fungi secrete various cellulolytic enzymes during the wood degradation process. The enzymes are known to be often appended with a cellulose binding domain (CBD) which assists the activity of catalytic domain. ...
Y Kojima, N Sunagawa, M Aoki, M Wada, K Igarashi, M Yoshida


The cellulose binding mechanism of a novel cellulose binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11021
In nature, wood decay is caused by various wood-rotting basidiomycetes. Wood-rotting basidiomycete are mainly divided into white-rot fungi and brown-rot fungi. Their main carbon source is cellulose of the wood cell wall during wood decay, and they produce a variety of enzymes to decompose cellulose. The cellulolytic enzymes often possess a cellulose binding domain (CBD) as an additional domain con...
M Aoki, Y Kojima, M Wada, M Yoshida


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