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Black Stain of Western red-cedar by Aureobasidium pullulans and its Relationship with Tropolone Depletion
2005 - IRG/WP 05-10564
Western red-cedar is valued for its natural durability conferred by fungicidal tropolone chemicals. However, weathered surfaces of WRC products are still susceptible to ‘black stain’ caused by fungi such as Aureobasidium pullulans. The effect of weathering on the tropolone content of heartwood was characterized and correlated with the ability of this fungal species to colonize the same weather...
R Chedgy, R Daniels, P I Morris, C Breuil


Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Integrated protection of freshly sawn lumber using Bacillus subtilis and selected fungicide
1997 - IRG/WP 97-10235
Bioprotection against stain fungi has tremendous potential for reducing discoloration of freshly sawn wood while decreasing chemical consumption. Unfortunately, most bioprotectants appear to be unable to consistently perform under the array of conditions to which freshly sawn wood is exposed. While research is underway to understand the nature of the inconsistent performance, a more pragmatic appr...
M E Mankowski, M Anderson, J J Morrell


Susceptibility of painted wood to discolouring fungi - influence of binder, solvent and surfactant
1992 - IRG/WP 92-3714
Previous studies have shown that the basic composition of paints, without fungicide addition, can significantly contribute to the varying microbial susceptibility of painted wood. Previously, non-weathered water-borne acrylic paints applied to wood without a primer were shown to be more susceptible to mould attack than solvent-borne alkyd paints. The present study was initiated to reveal if this d...
J Bjurman, C Herder


The selected properties of Scots pine wood blue-stained by fungus Cladosporium herbarum
2003 - IRG/WP 03-10484
The fungi causing primary blue-stain of wood may change wood properties especially when acting in mixture of few fungi species. There were been undertaken investigation to clear the influence of pure culture of one fungus species on selected physical and mechanical properties of Scots pine wood. The twin samples of fresh cut Scots pine sapwood (Pinus sylvestris L.) were exposed into pure culture o...
A Fojutowski


Comparison between two laboratory test methods for determining the effectiveness on wood preservatives against blue stain in fresh wood
1987 - IRG/WP 2289
Most of the work done on determination of the effectiveness of new formulae for treating fresh wood against blue stain have been focussed on their use in the manufacture of saw timber. This work explains two laboratory methods, one which simulates the working and climatological conditions of factories making packages for fruit and vegetables in the Spanish Levante, showing that contamination of wo...
A M Navarrete, M T De Troya


The use of preservative containing waste wood as substrate for growing greenhouse crops
1993 - IRG/WP 93-50011
In the Netherlands a large amount of waste wood and wood waste is produced every year. An important part of this amount comes from the pallet and packaging industries. One of the possibilities to re-use this relatively clean material is to convert it into substrates for growing crops in glass houses instead of the commonly used materials such as rock wool and glass wool. In this research, the infl...
W J Homan,H Militz


Blue stain in service on wood surface coatings. Part 1: The nutritional requirements of Aureobasidium pullulans
1992 - IRG/WP 92-1556
The nutritional requirements of Aureobasidium pullulans was examined with regard to nutrient sources that are potentially available in fresh and weathered wood. The study was designed to determine how far wood cell wall components need to be broken down during weathering before they provide a useful nutrient source for Aureobasidium pullulans. Various carbon sources were tested, with eight differe...
P R Sharpe, D J Dickinson


Laboratory studies on control of sapstain and mold on unseasoned wood by bacteria
1991 - IRG/WP 1493
A malt-agar and nonagar laboratory test was used to evaluate the efficacy of bacterial preparations as biological control agents against several sapwood-inhabiting fungi. Both nonsterilized and filter sterilized bacteria preparations prevented attack by the sapwood-inhabiting fungi. Streptomyces rimosus was the most effective bacteria, giving good protection, with a 1-hr soak in a filter-sterilize...
T L Highley, R Benko, S C Croan


Visualising Bacteria in Wood Using Confocal Laser Scanning Microscopy (CLSM)
1998 - IRG/WP 98-10272
A fluorescent phospholipid probe was used in conjunction with confocal laser scanning microscopy (CLSM), to visualise bacteria which inhabit in radiata pine wood and degrade pit membranes. CLSM has the ability to collect fluorescent images through different emission filters at the same time, so it is possible to distinguish gram-positive and gram-negative bacteria in infected wood by counterstaini...
Ying Xiao, A P Singh, R N Wakeling


Effect of nutrient regimes, temperature, pH, and wood sterilization method on performance of selected bioprotectants against wood staining fungi
1992 - IRG/WP 92-1551
The effect of nutrient regimes, incubation temperature, media pH, and wood sterilization method on performance of four potential bioprotectants (Pseudomonas putida, Pseudomonas cepacia, Bacillus subtilis, and Trichoderma harzianum) against wood staining fungi were evaluated using small ponderosa pine samples over a four week period. Incubation at 32°C resulted in slight increases in the degree of...
J J Morrell, C M Secton


Färbeverfahren zur Unterscheidung von Holzzonen mit unterschiedlichem Absorptionsvermögen, insbesondere bei Fichtenholz (Picea abies Karst.). [Staining method for distinguishing zones of wood with different absorption behaviour, particularly in spruce (Picea abies Karst.)]
1980 - IRG/WP 3163
A simple regressive staining method for crosscut wood surfaces is described. Phloroglucinol-hydrochloric acid serves as stain; flowing water and a solution of sodium carbonate are used for development and fixation of a picture showing the differences of absorption. Hydrophobic zones and, contrary to this, zones with a high water content can also be shown. Comparing green spruce wood before and dur...
S Cymorek


Performance of Tuff Brite C™ and other formulations against blue-stain, mold and brown-stain in freshly-sawn rubberwood (Hevea brasiliensis) in the humid tropics of Peninsular Malaysia
1997 - IRG/WP 97-30163
The relative anti-sapstain and anti-mold efficacies, including brown-stain development in freshly-sawn rubberwood (Hevea brasiliensis) in the Malaysian tropics, between selected water-based product concentrations of formulations Tuff Brite CTM without/with added Borax (at 1.5/0, 1/1.5, 1.5/1.5 and 2%/1.5%), PQ8TM without/with added Borax (at 2.5/0, 1.5/1.5, 2/1.5 and 2.5%/1.5%) and NeXgenTM/NeX-Br...
A H H Wong, T L Woods


A field test with anti-stain chemicals on sawn pine timber in Brazil
1989 - IRG/WP 3513
Field tests were carried out in São Paulo State - Brazil, to evaluate pre-treatment formulations of O-Phenyl-Phenol (OPP) based products. The Sodium Pentachlorophenate solution was used as a reference formulation. The treated test blocks were stacked in a covered shed, and in an uncovered place for air drying. The evaluation was done by measuring the percentage of stained surface on each test blo...
M B B Monteiro, S Brazolin


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Screening of modified linseed oils on their applicability in wood protection
2004 - IRG/WP 04-30346
In this study modified linseed oils, rape oil and three waxes were screened on their efficacy as wood protecting agents. By testing all products when impregnated with high retentions in Scots Pine sapwood on water repellence qualities, additionally an accelerated weathering test, drying quality, accelerated brown rot and blue stain test, an indication is formed of the capability of these products ...
A Treu, J Lückers, H Militz


Characteristics of the pigments produced by sap-staining fungi
1997 - IRG/WP 97-10223
The present study was conducted to characterize the pigments produced by sap-staining fungi, Aureobasidium pullulans (de Bary) Arnaud and Ceratocystis piceae (Münch) H. and P. Sydow. The pigments isolated from tested fungi were identified as melanins by spectroscopic methods (UV and infrared spectra). The UV and the infrared spectra of the fungal pigments were similar to those of the synthetic me...
M Mori, M Takahashi


The relationship between blue-stain and bark beetles
1971 - IRG/WP 19
The attack of bark beetles on standing or in newly-felled stems provides special growth conditions to wood-inhabiting fungi. In the wood attacked by bark beetles, a specific and rich fungus flora is found, and from these fungi the economically important group of blueing fungi has been more thoroughly investigated. These fungi live on nutritive substances present in the cells, especially in the med...
A Käärik


Molecular phylogeny of Ophiostoma spp. related to blue-stain in pine
2002 - IRG/WP 02-10432
Blue-stain in coniferous woods is an economically important disease in many temperate countries, caused specially by genus Ophiostoma. Classical taxonomy of genus Ophiostoma is limited for the correct identification of many species, because of the similar macroscopic and microscopic characters, as well as the existence of some species complex. Molecular identification is a good complement to eluci...
M Villarreal, F Arenal, J Sánchez-Ballesteros, M T De Troya, F Llinares, V Rubio, A Navarrete


New experiences with Dry Rot in Danish buildings, heat treatment and viability tests
1989 - IRG/WP 1423
An increasing number of concealed dry rot attacks, especially in older multi-storey houses under renovation, have been detected during the last few years. A necessary consequence is a demand for cheaper methods of repair, compared to the standard method used in Denmark for many years otherwise severely attacked houses will by pulled down. A heat-treatment has been developed and tested in practice....
A P Koch, C Kjerulf-Jensen, B Madsen


Suitability of propiconazole (R 49362) as a new-generation fungicide
1989 - IRG/WP 3529
Propiconazole (R 49362), an anti-fungal triazole-compound of Janssen Pharmaceutica, has been extensively tested to determine its potential use as a wood preserving fungicide. Various tests in both private laboratories and official institutes indicate a good activity against Basidiomycetes (brown and white rot) and Ascomycetes (blue stain-in-service). The active ingredient seems very resistant to w...
A R Valcke


The susceptibility of acetylated Pinus radiata to mould and stain fungi
1992 - IRG/WP 92-1548
The aim of this investigation was to determine the effect of 5, 10, 15 and 20 percent acetylation weight gains on the susceptibility of Pinus radiata sapwood to mould and stain fungi, and to establish if an acrylic paint, an oil-based stain and a water repellant gave superior protection from fungal growth on wood treated to 20% acetylation weight gain, compared to untreated wood. One treatment set...
R N Wakeling, D V Plackett, D R Cronshaw


Aureobasidium or Hormonema? A Genetic Approach.
2004 - IRG/WP 04-10529
Aureobasidium pullulans is the main organism causing disfigurement of coatings on wood and the surface of exposed timber. This disfigurement of timber in-service is referred to as “bluestain in-service”. A. pullulans is also associated with the sapstaining of dead wood in the forest and in-service. A. pullulans is noted for its highly variable growth forms (polymorphisms). This variability pre...
M J Ray, D J Dickinson, M Buck


The influence of staining fungi on the decay resistance of wood treated with alkylammonium compounds
1984 - IRG/WP 3308
Although found to be very effective in laboratory tests, alkylammonium compounds (AAC's) have failed to perform as well in field stake tests. Examination of leachability showed that this was not the cause. The present study investigated the possibility that staining fungi, (which have been observed to rapidly infect the field stakes), degrade the AAC wood preservative. Soil-blocks were tr...
J N R Ruddick


The influence of causing primary blue-stain fungus Ceratocystis imperfecta on selected properties of Scots pine wood
2002 - IRG/WP 02-10425
The effects of development in wood the fungi causing blue-stain on the wood properties are not univocal. There were been undertaken investigation to clear the influence of such fungi on selected physical and mechanical properties of Scots pine wood. Wet sap wood samples of fresh cut Scots pine wood (Pinus sylvestris L.) were infested with pure culture of Ceratocystis imperfecta Mill. et Grenz and...
A Fojutowski


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