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Termite and decay resistance of particleboard composed of white cypress pine and radiata pine
1997 - IRG/WP 97-10200
Phenol-formaldehyde bonded particleboard was manufactured from blends of non-durable radiate pine (P. radiate) and heartwood of the naturally durable species, white cypress pine (C. glaucophylla). Board specimens were subjected to bioassays using two termite species, M. darwiniensis and C. acinaciformis, and the basidiomycete fungi, C. puteana and P. ostreatus, and the durability of specimens was ...
P D Evans, J W Creffield, J S G Conroy, S C Barry


Attempt for developing a new method for above ground field testing of wood durability
2000 - IRG/WP 00-20199
Field testing remains the most appropriate way for evaluation of wood preservatives or natural durability of wood species. Above ground tests are designed to answer specific questions concerning the outdoor utilisation of wood. Such methods supply additional information and confirm or reject preliminary laboratory results. The European standard (ENV 12037, "lap-joint") for assessment of the durabi...
N Terziev, M-L Edlund


Reaction of osmium tetroxide with a diphenolic compound as a model substance for demethylated lignin
1986 - IRG/WP 1303
By using caffeic acid as a model substance for diphenolic moieties of demethylated lignin it was found that electron dense particles called by the authors "osmiophilic particles" in former papers are reaction products of OsO4 with the model substance. By these products it is possible to indicate the enzymatic demethylation of lignin taking place during wood decay. The appearance of the electron de...
K Messner, E Srebotnik


Susceptibility of CCB treated wood to fungal colonization
2003 - IRG/WP 03-10492
CCB treated wood is generally resistant to all wood decay fungi. However, like CCA impregnated wood, susceptibility of CCB treated wood to copper tolerant fungi have been observed. The ability of various brown rot fungal hyphae to penetrate and overgrow the wood samples was investigated. Samples made of Norway spruce (Picea abies) were impregnated with 5 % CCB solution according to the EN 113 proc...
F Pohleven, U Andoljsek, P Karabegovic, C Tavzes, S A Amartey, M Humar


Evaluation of fungal remediation of creosote treated wood
1998 - IRG/WP 98-50101-25
Biotechnological remediation of creosote treated wood may be of interest in connection with novel recycling processes. White rot fungi and/or their ligninolytic enzyme systems are supposed to be valuable tools for such processes. This paper reports about results achieved when creosoted wood was treated in solid substrate fermentation with selected white rot fungi after different extraction procedu...
K Messner, S Böhmer


Evidence for wood cell wall degradation by the blue stain fungus Botryodiplodia theobromae Pat
1994 - IRG/WP 94-10077
Botryodiplodia theobromae Pat., a world wide ubiquitous polyfagus sapstain fungus, was found able to destroy the cell walls of birch fibres (Betula verrucosa Ehrh.) but not Caribbean (Pinus caribaea var. hondurensis Barr. and Golf.) and Scots pine (Pinus sylvestris L.) tracheids. The fungus caused characteristic erosion of fibre cell walls similar to soft rot type 2; destruction of the S1 - S2 int...
O Encinas, G F Daniel


The efficacy of Sentry® as a treatment for the control of sapstain in pre-infected radiata pine
2001 - IRG/WP 01-30260
Experiments were conducted to determine the efficacy of Sentry® when used to control sapstain fungi on radiata pine branch billets stored under high fungal hazard conditions. Prior to antisapstain treatment, the billets were pre-infected with a mixed inoculum of staining fungi and held from zero to 10 days at 25°C and 75% relative humidity. This was done to produce billets with different degrees...
C Chittenden, J G van der Waals, B Kreber, R N Wakeling


Comparative performance of several ammoniacal copper preservative systems
1997 - IRG/WP 97-30151
The efficacy of several ammoniacal copper-based wood preservative systems was evaluated in this study. The selection of potential co-biocides was based on the results of an agar plate test. Following this, the most promising systems were evaluated in a standard field stake test. Good correlation was found between the agar plate and field stake test results. Of the preservative systems tested, copp...
D D Nicholas, T Schultz


The effect of tunicamycin on production and secretion of extracellular carbohydrate-degrading enzymes by Postia placenta
1988 - IRG/WP 1342
The extracellular carbohydrate-degrading enzymes of wood-decay fungi are usually heavily glycosylated and therefore stable under most denaturing conditions. It is unlikely that wood decay can be prevented by simply inactivating these enzymes. Tunicamycin, an antibiotic produced by Streptomyces lysosuperificus, prevents the glycosylation of glycoproteins and can interfere with the secretion of thes...
J A Micales, T L Highley


Using DNA probes to characterize the metabolic pathway of pigment production in several wood-staining fungi
1996 - IRG/WP 96-10146
During shipment and storage, lumber is susceptible to sapstain, a wood discoloration caused by fungi. Currently kiln drying and chemical applications are used to control sapstain. However, the chemicals used to protect wood have a broad range of action, and so can affect other organisms. In addition, in Canada most of these chemicals are under temporary registration. Thus there is a need to develo...
R Eagen, S Riecken, J Kronstad, C Breuil


In vitro sporulation of selected wood decay fungi
1978 - IRG/WP 190
Basidiospores produced axenically without laborious attention in the laboratory are useful in studies of wood decay initiation. Such spores presumably approach those collected from natural sporophores in size and germinability (Morton, 1964). Production of spores in vitro by inversion of cultures grown on 2% malt extract agar in deep glass dishes (100x80 mm²) has been the preferred method (Morton...
E L Schmidt, D W French


Wood extractives as carbon sources for staining fungi in the sapwood of lodgepole pine and trembling aspen
1995 - IRG/WP 95-10098
Wood discoloration by sapstaining fungi reduces lumber value. In Canada, the most commonly recorded sapstaining fungi belong to the genera Ophiostoma. This work evaluated the effect that Ophiostoma piceae, Ophiostoma ainoae, and Ophiostoma piliferum used the lipids present in the sapwood of lodgepole pine (Pinus contorta Dougl.) and trembling aspen (Populus tremuloides Michx.). Wood lipids, common...
Yong Gao, C Breuil


Étude in vitro de la colonisation et de la dégradation structurale du bois d'aubier de Pin sylvestre par la Mérule: Serpula lacrymans Schum. ex Fr. S. F. Gray
1979 - IRG/WP 198
The degradation of Scots pine sapwood cell walls by Serpula lacrymans, a brown rot fungus, is observed after various periods of exposure from two weeks to twelve weeks. The observation by microscopy shows that the hyphae of Serpula rapidly invade the wood tissues as cell wall degradation starts. That deterioration is not gradual, it is observed to be very irregular as well within the whole of the ...
D Dirol


Moisture requirements and wood degradation of pine and spruce wood by some Basidiomycetes-fungi
1989 - IRG/WP 1406
The minimum moisture requirements for the growth of the brown rot fungi, Coniophora puteana, Gloeophyllum trabeum and Serpula lacrymans were near or above the fibre saturation point of pine and spruce, sap and heartwood, during 160 days incubation according to the standard method EN 113. The moisture of wood for the optimum growth of Coniophora puteana and Serpula lacrymans was about 35-80% and fo...
H Viitanen, A-C Ritschkoff


Durability of Wood Plastic Composites Relative to Natural Weathering and Preservative Treatment With Zinc Borate
2005 - IRG/WP 05-40316
Wood-Plastic Composites (WPCs) used for decking have experienced dramatic increases in North America, averaging 25% growth per annum since 1998. A key factor contributing to this growth has been the successfully communicated message that they are "virtually maintenance free". The common perception being that the wood fiber is completely encapsulated by the thermoplastic resin, minimizing the pot...
M E Mankowski, F M Ascherl, M J Manning


Using image analysis to rate wood stain trials
1993 - IRG/WP 93-10034
Assessing the extent of fungal discoloration on wood during laboratory trials is a subjective process which provides an opportunity for considerable variation between individual evaluators of specimens. The development of reliable systems for automatically assessing discoloration would minimize evaluator bias and permit comparisons between results from different laboratories. The use of image anal...
C M Sexton, A G Maristany,C C Brunner, J J Morrell


Decrease in the mechanical resistance of blue stained and "orange stained" Pinus radiata wood
1999 - IRG/WP 99-10313
It is recognised that sapwood attacked by Ceratocystis pilifera increases its permeability to pressure preservative treatments. Since hiphae not only utilise pits, but also the cell wall for crossing from one cell to another, the mechanical resistance of the stained wood might be affected. The orange colour very commonly present in Pinus radiata logs heartwood under water sprinklers, has not recei...
M C Rose, L Reyes, J Alvarez, A Andaur, C Beltran, D Rivera


Fungal-termite associations in the natural resistance of selected eucalypt timbers
1978 - IRG/WP 173
Butt billets of Eucalyptus regnans F. Muell., Eucalyptus grandis W. Hill ex Maiden, and Eucalyptus camaldulensis Dehnh., showing no visible signs of decay, were examined for the presence of fungi in outer, middle and inner heartwood. Fungi obtained in pure culture were characterized. Eucalyptus regnans yielded Cytospora eucalypticola van der Westhuizen, Trichoderma harzianum Rifai and Penicillium ...
D B A Ruyooka


Molecular analysis of the basidiomycete Coniophora puteana
1992 - IRG/WP 92-1534
Sodium dodecylsulphate polyacrylamide gel electrophoresis and Western Blotting, using a polyclonal antiserum produced against a whole cell extract of Coniophora puteana, were used to analyse the major proteins and antigens of the wet rot organism Coniophora puteana. The macromolecule profiles of this organism were different from other members of the Coniophora genus and from a set of unrelated org...
H E McDowell, D Button, J W Palfreyman


Changes in pore structure and cell wall volume in wood decayed by brown- and white-rot fungi
1991 - IRG/WP 1501
Sweetgum (Liquidambar styraciflua L.) wood blocks were decayed by Postia (=Poria) placenta or Phanerochaete chrysosporium in soil-block cultures. Decay was terminated at various weight losses, and the pore volumes available to probes of various molecular weight and diameter were determined by the solute exclusion technique (Stone, J.E. and A.M. Scallan. 1968. Cellulose Chem. Technol. 2, 343-358.)....
D S Flournoy


The fungal degradation of quaternary ammonium compounds in wood
1998 - IRG/WP 98-10263
This work focuses on the biodegradation of didecyldimethylammonium chloride (DDAC) by the mould fungus Gliocladium roseum within a woody matrix. Three sawdust types, distinguished by their treatment and amount of DDAC retained, were inoculated with the fungus and their DDAC loss was measured over 11 weeks. The rates of degradation varied depending on the sawdust; however, a significant loss of DDA...
J W Dubois, J N R Ruddick


The effect of different concentrations of polesaver rods on the survival of selected decay fungi in liquid culture
1998 - IRG/WP 98-30166
An in-vitro method was developed to determine the effect of solutions of Polesaver Rods on the survival of selected decay fungi. Polesaver Rods are a diffusing preservative treatment containing fluorine and boron designed for the internal remedial treatment of power poles. Two white-rot fungi, Perenniporia tephropora and Trametes versicolor, and two brown-rot fungi, Polyporus verecundus and Gloeo...
M A Powell, T Deldot, C McEvoy


Preliminary studies of the decay mechanism of some brown-rot fungi
1989 - IRG/WP 1402
The importance of the enzymatic degradation of hemicellulose and cellulose by brown-rot fungi is still under discussion. Endo-ß-1,4-xylanase and endoglucanase activities of Coniophora puteana and Poria placenta cultures were measured by the increase in reducing groups. Enzymes were produced in liquid and solid sawdust based culture media. Enzyme activities were measured in two, four and eight wee...
A-C Ritschkoff, H Viitanen


An attempt to develop a direct and reliable method for testing the preventive action of preservation treatments of wood against fungal decay
1980 - IRG/WP 2139
In wood preservation there are two classical ways for assessing the reliability of preventive treatments against wood decay: the laboratory tests in which the various parameters are evaluated independently and the field tests or service tests in which those parameters are acting together in the natural environment. One has always tried to build bridges between the two types of experiments and to e...
M Fougerousse


Soft rot decay of Belian (Eusideroxylon zwageri) wood
1995 - IRG/WP 95-10119
The heartwood of Belian (Eusideroxylon zwageri), an East Malaysian timber species, is naturally durable due to its certain unique anatomical features and high content of extractives. The timber can tolerate years of exposure to hazardous conditions in ground contact and other situations without any significant loss in its strength. A few Belian transmission poles sampled from one locality in Saraw...
A H H Wong, A P Singh


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