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Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner


Investigation of extracellular mucilaginous material in some wood decay fungi
1996 - IRG/WP 96-10188
The external morphology of the extracellular mucilagenous material (ECM) produced by Coriolus versicolor and Coniophora puteana during colonization of Scots pine and beech was studies using SEM. Specimens were examined in the frozen hydrated, freeze-dried and critical point dried state. All technics produced artefacts but the ECM was best preserved when examined the frozen hydated state. Critical ...
A R Abu, D J Dickinson, R J Murphy


Silicon tetrachloride: A potential wood preservative
1980 - IRG/WP 3133
In its present form, this paper is a preliminary draft of a paper that will eventually be submitted for publication in the Forest Products Journal. We are continuing the work on the effects of silicon tetrachloride on wood and expect to have additional data for the meeting in May 1980. Specifically we are measuring the penetration rate and depth of SiCl4, and we are chemically analyzing the treate...
C W Owens, W T Shortle, A L Shigo


Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


Inhibitory effects of leachates from Scots pine wood on germination of some wood rotting fungi
1986 - IRG/WP 1282
Leachates from sapwood of Pinus sylvestris inhibited or reduced the germination of basidiospores of the wood decay fungi tested. The fungi were selected among those preferentially colonizing hardwood or softwood, representatives for brown rot and white rot fungi, early basidiomycete colonizers and late basidiomycete colonizers in above-ground parts of pine as well as fungi found in high frequencie...
J Bjurman


Penetration as Key Factor for Determining the Performance of Wood Preservatives
2004 - IRG/WP 04-20293
Stakes of Scots pine sapwood with a dimension of 4 x 4 x 75 cm³ were impregnated with preservatives containing 2 % of propiconazole which had passed the EN 113 test criteria without any problems. The penetration of the colourless preservative was determined successfully by a fungal screening test described by Sutter (1978). The test revealed the insufficient penetration behaviour within the gi...
H Leithoff, I Stephan, R-D Peek


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe


Heat inactivation of mould fungi on wood
1993 - IRG/WP 93-40012
Heat treatments have been successfully used for eradication of dry rot. The effect of heat treatments at 40, 50 and 60°C for various time intervals on the inactivation of Penicillium brevicompactum was determined. The effect of heat on ungerminated spores and spores "pregerminated" for 24 hours was evaluated by plating on malt-extract agar. Although most ungerminated spores were inactivated after...
J Bjurman


The relationship between blue-stain and bark beetles
1971 - IRG/WP 19
The attack of bark beetles on standing or in newly-felled stems provides special growth conditions to wood-inhabiting fungi. In the wood attacked by bark beetles, a specific and rich fungus flora is found, and from these fungi the economically important group of blueing fungi has been more thoroughly investigated. These fungi live on nutritive substances present in the cells, especially in the med...
A Käärik


Natural Durability studies and Changes in wood chemistry of some Ghanaian hardwoods during decay by white- and brown-rot fungi
2004 - IRG/WP 04-10542
The natural decay resistance of Esa (Celtis mildbraedii), Wawabima (Sterculia rhinopetela) and Dahoma (Piptadeniastrum africanum) obtained from Ghana was determined according to BS EN 113 (1997). Esa was found not durable to the white-rot, but very durable to the brown-rot; Wawabima was very durable to both white- and brown-rots; and Dahoma was durable to the white-rot, and very durable to the bro...
Zeen Huang, K Maher, S A Amartey


Screening wood preservatives: Comparison of the soil block, agar block and agar plate tests
1993 - IRG/WP 93-20001
Several test procedures have been developed over the years to screen potential biocides for their value as wood preservatives. Each test has inherent advantages and disadvantages. In this paper the relative merits of the soil block, agar block and agar plate tests are compared. Eight commercially available biocides encompassing inorganic and organic systems were tested against four basidiomycete d...
K J Archer, D D Nicholas, T Schultz


Biological control of wood decay fungi. - Part II. Effects of exogenous nitrogen on effectiveness
2000 - IRG/WP 00-10360
The effects of nitrogen (urea and ammonium nitrate) on the ability of Trichoderma harzianum to inhibit the enzyme capabilities and decay capacities of Trametes versicolor and Gloeophyllum trabeum were investigated using a small wood sandwich decay test. The biocontrol fungus generally had a greater effect on the brown rot fungus, confirming previous reports. Nitrogen produced inconsistent effects ...
E A Canessa, J J Morrell


The colonisation and succession of fungi in wood
1980 - IRG/WP 1107
A study of the microbial ecology of small stakes of Pinus sylvestris and Betula pendula, both untreated, and treated with a 1% solution of CCA is in progress. Results are presented for the early stages, up to 18 months.Two new techniques were developed linked by the philosophy of objective assessment. Isolations were made from the wood in a pre-determined pattern and transferred to four selective ...
C P Clubbe


Biosorption of metals for wood waste effluent clean up
1997 - IRG/WP 97-50090
The common filamentous fungi can sorb metal ions from aqueous solutions. The biosorption of metal ions like copper Cu2+, chromium Cr6+ and arsenic As5+ by dead fungal mycelia was investigated. Two industrial fermentation byproducts are tested: Mucor miehei and Aspergillus oryzae. The adsorption of metal ions depends on parameters such as chemical treatment of fungi before biosorption (acid or basi...
S Legay, G Labat


Eradication of wood decay fungi by means of radio frequency
1998 - IRG/WP 98-10292
High frequency electromagnetic fields i.e. radio frequency (RF) are used in wood industry for heating, gluing and bending of wood and are also appropriate for eradicating of wood decay fungi and insects. We investigated the effects of RF exposure on wood samples which were in vitro infected by Coniophora puteana, Gloeophyllum trabeum and Lentinus lepideus. For each fungus, the lethal temperature a...
F Pohleven, J Resnik, A Kobe


The influence of staining fungi on the decay resistance of wood treated with alkylammonium compounds
1984 - IRG/WP 3308
Although found to be very effective in laboratory tests, alkylammonium compounds (AAC's) have failed to perform as well in field stake tests. Examination of leachability showed that this was not the cause. The present study investigated the possibility that staining fungi, (which have been observed to rapidly infect the field stakes), degrade the AAC wood preservative. Soil-blocks were tr...
J N R Ruddick


Analysis of volatile organic compounds (VOC's) from Trichoderma spp.: effect of media composition on VOC production and level of inhibition of wood decay fungi
1997 - IRG/WP 97-10207
Production of volatile organic compounds (VOC's) by two Trichoderma isolates grown on either malt extract or minimal media was examined and statistically assessed to identify which VOC's were predominantly produced on each of the two media types. The VOC's were captured onto chromatographic absorbent from above the cultures of the fungi before being analysed by integrate...
A Bruce, R E Wheatley, C Hackett, A Kundzewicz


Environmentally benign wood preservatives based on organic biocide antioxidant combinations: A brief review of laboratory and field exposure results and discussion of a proposed mechanism
2004 - IRG/WP 04-30335
The combination of various organic biocides with commercial antioxidants generally increased the biocides’ efficacy 2-3 fold against wood-destroying fungi in short-term laboratory decay tests, and some positive results have been obtained after 2-4 years of outdoor exposure. The two antioxidants principally examined, propyl gallate and butylated hydroxytoluene, are low cost and, since both are f...
T Schultz, D D Nicholas, W Henry, C Pittman, D Wipf, B Goodell


Effect of Pseudomonas cepacia on the activity of a mixture of wood staining fungi on ponderosa pine sapwood
1995 - IRG/WP 95-10107
There are major incentives for limiting the degree of fungal discoloration which occurs during wood processing. Most lumber producers apply prophylactic fungicides which limit microbial growth, but increasing restrictions on the use of these chemicals have encouraged the search for alternative stain prevention strategies. Among the possible strategies for stain prevention is biological control. Wh...
J J Morrell, R K Velicheti


The effects of preservative treatment and exposure to wood degrading fungi on fiber reinforced polymer (FRP) materials used for structural wood reinforcement
2001 - IRG/WP 01-40204
Glass fiber reinforced phenolic (GFRP) composite materials are becoming increasingly accepted for use in the construction industry because they combine advantages of both wood and advanced polymeric materials. Addition of only 1-3% FRP in the tension zone, for example, can typically improve the strength of the hybrid system by 200%. As more applications are found for wood/FRP hybrids, (e.g. lamina...
C Tascioglu, B Goodell


A laboratory evaluation of tributyltin ethanesulphonate as an aqueous fungicide in wood preservation
1983 - IRG/WP 3229
Toxic limit data, using both leached and unleached wood test blocks, are reported for aqueous solutions of tributyltin ethanesulphonate against four Basidiomycete fungi, Poria placenta, Gloeophyllum trabeum, Coriolus versicolor and Coniophora puteana. An in situ study of the tributyltin compound in Scots pine and ponderosa pine sapwood has been carried out, using the technique of 119mSn Mössbauer...
R Hill, P J Smith, J N R Ruddick, K W Sweatman


EPR observation of Mn(II) in decayed wood
2002 - IRG/WP 02-10443
Preliminary Electron Paramagnetic Resonance (EPR) studies of copper/chromium (CC) treated spruce wood, subjected to laboratory soil decay test showed increased intensities of Mn(II) EPR signal in partially decayed specimens. Several reasons might be responsible for this phenomenon: Mn(II) could originate from the wood after being released from the typical ligand binding, from reduction of other Mn...
M Petric, M Humar, P Kalan, F Pohleven, U Schoknecht, M Šentjurc


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