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Morphological observation of wood at the early stages of decay in brown rot and white rot
2023 - IRG/WP 23-11020
Wood rotting fungi, the fungal species causing biodeterioration for wood building, are generally classified into white-rot, brown-rot and soft-rot fungi based on their decay modes. Since white-rot and brown-rot fungi are known to reduce wood strength significantly, it is important to clarify the mechanisms of their wood degradation. White-rot fungi reduce wood strength as the decay progress and de...
R Tsukida, T Hatano, Y Kojima, Y Horikawa, S Nakaba, R Funada, M Yoshida


Effect of volatile organic compounds produced by wood rotting fungi on mycelial growth
2023 - IRG/WP 23-11023
Microorganisms such as fungi or bacteria produce volatile organic compounds (so-called MVOCs: Microbial Volatile Organic Compounds) as metabolites. Some MVOCs have been found to be biologically active, for example inhibition of spore germination, and when fungi of different species encounter each other, the MVOCs pattern change, suggesting that MVOCs play a role as a mediator for biological intera...
S Horikawa, R Konuma, M Yoshida


Comparison of culturable fungi found on two types of modified wood cladding
2023 - IRG/WP 23-11025
Modified woods are rapidly gaining popularity in the United States and are appearing on more public buildings. Because these are biocide-free products, they can support the growth of non-wood degrading fungi on their surfaces, many of which produce dark pigments and deface the cladding. Even growth of dark pigmented fungi on modified woods yields an even grey-black color, giving the materials a we...
G Presley, J Cappellazzi, B Held, R Blanchette


Simulations of microclimates for wood-decaying fungi in the built environment using environmental analysis
2023 - IRG/WP 23-20703
Simulations of fungal decay risk were run on two similar building geometries exposed to typical annual climate conditions of two different geographical locations, Brunswick (Germany) and Cairns (Australia). The simulations were conducted to capture the effect of wind-driven rain and solar irradiation exposure over nodes of the common building geometry. The moisture content and temperature variatio...
P B van Niekerk, J Niklewski, S H Hosseini, B N Marais, I Frimannslund, T Kringlebotn Thiis, C Brischke


Research on gaseous COS degradation by brown-rot fungus Gloeophyllum trabeum
2024 - IRG/WP 24-11033
Physiological studies of wood rotting fungi have mostly focused on the metabolism of carbon and nitrogen sources, which constitute the dominants components of wood. On the other hand, despite the physiological importance of trace elements such as sulfur, studies on their acquisition sources and metabolic pathways are limited. Until now, wood rotting fungi have been thought to utilize slight amount...
R Iizuka, R Tsukida, Y Katayama, M Yoshida


Cellulose nanomaterials in growth media for wood decay fungi
2024 - IRG/WP 24-11035
Cellulose nanofibrils (CNFs) were investigated as a partial substitution for agar in growth media for wood decay fungi. Radial growth measurements of eight basidiomycete fungi were taken on solid growth media with and without CNFs. Additionally, fungal strain virulence was evaluated using the European CSN Standard EN 113-2 wood decay durability test. The inclusion of CNFs did not significantly aff...
K M Ohno, R A Arango, R Sabo, C M Clemons, G T Kirker, A B Bishell


Cellulose nanomaterials in growth media for wood decay fungi
2024 - IRG/WP 24-11035
Cellulose nanofibrils (CNFs) were investigated as a partial substitution for agar in growth media for wood decay fungi. Radial growth measurements of eight basidiomycete fungi were taken on solid growth media with and without CNFs. Additionally, fungal strain virulence was evaluated using the European CSN Standard EN 113-2 wood decay durability test. The inclusion of CNFs did not significantly aff...
K M Ohno, R A Arango, R Sabo, C M Clemons, G T Kirker, A B Bishell


On the use of miniaturized wood specimens in fungal decay experiments – mini-blocks versus EN 113 test specimens
2024 - IRG/WP 24-11044
Preliminary evaluation and classification of wood durability against decay fungi using laboratory monoculture experiments has been a longstanding practice because these tests offer quicker results than field tests. Various methods, including miniaturized specimen formats and different incubation periods, have been explored to expedite decay processes and assess wood preservation efficacy. The 'min...
C Brischke, G Alfredsen


A novel cellulose-binding domain from the brown-rot fungus that can be used to evaluate cellulose in wood
2024 - IRG/WP 24-11046
Wood-rotting basidiomycetes are the primary microorganisms that decay wood in nature. They are classified as white-rot fungi and brown-rot fungi by the difference in decaying types. White-rot fungi secrete a variety of cellulolytic enzymes during wood degradation. These enzymes often have an additional cellulose-binding domain (CBD) that adsorbs to the cellulose surface and localizes the catalytic...
Y Kojima, N Sunagawa, S Tagawa, T Hatano, S Nakaba, M Aoki, M Wada, K Igarashi, M Yoshida


Potential of heartwood extracts as wood preservatives combined with linseed oil in 8-year above-ground and in-ground field trials
2024 - IRG/WP 24-20719
The current study assessed the efficacy of heartwood extracts from Tectona grandis, Dalbergia sissoo, Cedrus deodara, and Pinus roxburghii, combined with linseed oil, as protectants for two non-durable wood species against termites and decay fungi. Stakes measuring 45.7 × 1.9 × 1.9 cm and blocks measuring 12.5 × 3.75 × 2.5 cm of the sapwood of southern pine (Pinus sp.) and cottonwood (Populus ...
M E Mankowski, G T Kirker, B Hassan


Changes in decay risk of wood in ground contact over Europe, from analysis of historic climate conditions
2024 - IRG/WP 24-41008
Changing climate conditions will affect global temperature and precipitation patterns, shifting some geographical areas into states that are more suitable for fungal wood decay. Climate normals, which are taken over a 30-year period, capture vast spatial and temporal variations in these conditions and comparing these can show changes over time. Importantly, being able to identify locations that ha...
P B van Niekerk, B N Marais, G Alfredsen, C Brischke


Towards eco-friendly fire retardant of wood - exploring fungal biomineralization
2025 - IRG/WP 25-11052
Timber is a strong, durable, aesthetically pleasing and sustainable building material but its combustibility represents a safety threat. Commonly used fire and flame retardants are often environmentally unfriendly, while safety cladding is labour intensive and covers the wood, decreasing the aesthetic appeal of the building. Mineralisation of wood with calcium carbonate has been shown to be a prom...
K Butina Ogorelec, A Gubenšek, A Sandak


Synthetic Oxalate/ß-glucan Fungal Extracellular Matrix Demonstrates Potential Inhibition of Extracellular Enzyme Diffusion into Wood Cell Walls, and Calls into Question the Role of Enzymes in Wood Decay
2025 - IRG/WP 25-11053
ß-glucan is the major component of the extracellular matrix (ECM) of many fungi, including wood degrading fungi. Many of these species also secrete oxalate into the ECM. Our research demonstrates that ß-glucan forms a novel, previously unreported, hydrogel at room temperature with oxalate. This finding better explains the gel-like nature of the fungal ECM. Oxalate, at relatively low levels, was ...
B Goodell, G A Tompsett, G Perez-Gonzalez, K Mastalerz, M Timko


Decay pattern in external wooden structures
2025 - IRG/WP 25-11055
Decay in external wooden structures has historically been a universal problem of great concern. Despite this, the general understanding of where and why wood-decaying fungi are occurring is limited. The consequences of this are that decayed wood might not be detected and that measures to protect the wood against deterioration has limited effect. Wood decay in different geographical and climatic ...
J Mattsson, M S Austigard, A-C Flyen


Xerophilic mould fungi on new and historic wood
2025 - IRG/WP 25-11056
An examination of mould fungi in 35 medieval and ten 18th Century buildings shows mould growth to varying degrees on different surfaces. The analyses also show a rapid development of mould fungi on certain wooden surfaces in recent years. A significant difference in the growth between new and old wood is the clearly more extensive growth and different species composition of mould fungi on new wo...
J Mattsson, M S Austigard


Durability to wood decay fungi and subterranean termites of Quercus pyrenaica Wild
2025 - IRG/WP 25-11066
The use of wood has added value since it is renewable, biodegradable, and a recyclable material. For this reason, evaluating its natural durability is essential when establishing the level of protection required, depending on the designated end use. Natural durability is defined as the intrinsic resistance of wood against the attack of destructive organisms. There are standardised methods to estim...
S Santos, M T Troya, L Robertson, S Gutierrez, G Caballe, J L Villanueva


Durability and molecular analysis of fungal communities in wood exposed above ground
2025 - IRG/WP 25-11068
The objective of the study was to explore the durability of wood exposed above ground, comprising a wide diversity of wood species and treatments and extensive assessment data on wood durability in ongoing long-term testing. Larch species and Scots pine heartwood, two tropical species ntholo and ncurri and a number of treated wood by copper-based formulations and alternatives (silanes, spiroborate...
N Högberg, D Panov, G Daniel, N Terziev


Detecting wood-decay fungi in walls: a non-destructive approach with an electronic nose
2025 - IRG/WP 25-11070
Wood-decay fungi degrade the structural integrity and safety of wooden buildings, leading to potential hazards and reduced durability. Conventional detection methods, such as visual inspection and destructive sampling, are often impractical for concealed spaces, such as those within walls or beneath floors. These methods often require specialised training and costly equipment, making non-destructi...
M Suzuki, T Miyauchi, S Isaji, R Naganawa


Interaction of cellulolytic enzymes with fungal cell wall polysaccharides
2025 - IRG/WP 25-11071
Wood rotting fungi are the primary agents responsible for the decomposition of wood in natural environments. These fungi secrete a variety of enzymes to degrade the chemically and structurally resistant components of the wood cell wall. Some of these enzymes possess a carbohydrate-binding module (CBM), which enhances enzymatic efficiency by increasing their affinity to the substrate. Previously, w...
K Fukabori, N Hattori, Y Kojima, R Iizuka, M Yoshida


Distribution of Crystalline Cellulose-Binding Domain CBM104 in Wood Rotting Fungi
2025 - IRG/WP 25-11073
Wood rotting fungi play a crucial role in biodeterioration of wood. Many brown-rot fungi are known to rapidly degrade cellulose in wood despite lacking enzymes with cellulose-binding domains. This has led to the hypothesis that they rely on a non-enzymatic degradation system. We recently discovered a novel cellulose-binding domain, CBM104, in the brown-rot fungus Gloeophyllum trabeum. In this stud...
Y Kojima, N Sunagawa, M Aoki, S Tagawa, M Wada, K Igarashi, M Yoshida


Investigation of oxalic acid production in brown-rot fungi
2025 - IRG/WP 25-11074
Brown-rot fungi play a crucial role as wood decomposers in forests and are also known as the main cause of biodeterioration of wooden buildings in Japan. Therefore, understanding their decomposition mechanisms is important for the maintenance and preservation of wooden structures. While white-rot fungi generally use enzyme-system for degradation, many brown-rot fungi lack cellobiohydrolases which ...
S Matsumoto, R Tsukida, T Ito, M Iritani, M Yoshida


Synergistic effect of the association of Prosopis juliflora polyphenolic extractives with tebuconazole on the growth inhibition of brown and white rot fungi: a solution to increase the naturality and safety of wood preservation treatment
2025 - IRG/WP 25-20732
The antifungal effect of catechin and extractives from Prosopis juliflora was studied against one white rot fungus, Trametes versicolor (TV), and one brown rot fungus, Rhodonia placenta (PP). The extractives from Prosopis julilfora were crude mesquitol and pure mesquitol. Tebuconazole was used in this study as a known fungicide against the two named fungi. Wood protection using fungicides can be h...
J Owino, J Tuimising, F Mangin, P Gérardin, A Kiprop, C Gérardin-Charbonnier


Potential co-valorization of pyrolysis tars from five wood species form Tunisia as wood preservatives bases: Chemical compositions, antifungal and anti-termite activities
2025 - IRG/WP 25-20739
The pyrolysis of five Tunisian wood species (Ceratonia siliqua, Eucalyptus camaldulensis, Eucalyptus maidenii, Pinus pinea, and Quercus canariensis) was carried out in a custom-designed carbonisation reactor developed at the INRGREF's Wood Technology Section (Tunis, Tunisia). This process aimed to produce both char and pyrolysis by-products, particularly pyrolysis oils. The experiments were conduc...
M T Elaieb, J Valette, E Kiény, L Pignolet, S Ghariani, K Candelier


Properties of plasma etched super black wood
2025 - IRG/WP 25-30825
Super-black materials have very low reflectivity due to structural absorption of light. They are attracting considerable scientific and industrial attention because of their important applications in many fields including astronomy, photovoltaics, and optical science, amongst others. We have developed the first super-black material from wood by etching transverse basswood (Tilia americana L.) surf...
K J Cheng, X Xu, V A Hernandez, P D Evans


Danish wood preservatives approval system with special focus on assessment of the environmental risks associated with industrial wood preservatives
2001 - IRG/WP 01-50166-01
The following is a description of the procedure used by the Danish Environmental Protection Agency to assess the environmental risks associated with preservatives used in the pressure impregnation of wood. The risk assessment covers issues considered to be of significance for the environment and which are adequately documented so as to allow an assessment. Such issues are persistence and mobility ...
J Larsen


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