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Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell


Preservation of basidiomycete hyphae in ancient waterlogged wood materials
1992 - IRG/WP 92-1536
Studies on waterlogged archaeological wood show that basidiomycete hyphae may persist as long as 800 years. In two pine wood samples with Phellinus pini heartrot, one from the foremast of the ship Vasa and the other from a bulwark constructed in the first part of the 12th century, numerous resin covered hyphae were observed. Hyphae with clamp connections that were associated with brown and white r...
T Nilsson, G F Daniel


In vitro antifungal activity of chilli against wood degrading fungi
2006 - IRG/WP 06-10572
The efficacy of chilli juice and/or chilli extract oleoresin as antisapstain agents was evaluated against two common sapstain fungi, Sphaeropsis sapinea and Leptographium procerum. Possible synergy between chilli juice and Lactobacillus casei as antisapstain agents was also assessed. Both the chilli juice and the oleoresin showed moderate antifungal activity. No growth of the test fungi was obse...
T Singh, C Chittenden, D Vesentini


Biological variability in the oxalate/oxalate decarboxylase system among five isolates of the wood-degrading fungus Meruliporia incrassate
2006 - IRG/WP 06-10573
The “dry-rot” wood decay fungus Meruliporia incrassata has recently attracted attention, primarily in the western coastal United States, as a particularly destructive pest of building material. Recently, the US Environmental Protection Agency (EPA) has accepted a voluntary withdrawal of the historically effective chromated-copper arsenate (CCA) as a preservative for wood used in residential se...
C Howell, J Jellison


Comparing microbial colonisation and Decay Rates of Wood from Sound and Aphid-Killed Kenyan-Grown Mexican Cypress (Cupressus lusitanica)
2006 - IRG/WP 06-10599
Samples of wood from 10, 15, and 30-year old trees attacked and killed by the cypress aphid (Cinara cupressi) and sound Kenyan-grown Cupressus lusitanica trees were investigated for variations in moisture content, density and susceptibility to microbial decay. MC varied with tree age, a normal trend, and between samples from aphid-killed and sound trees. In samples from 10, 15 and 20-year old so...
R Venkatasamy


Antifungal activity of essential oils against common wood degrading/decaying fungi
2008 - IRG/WP 08-30465
Despite the wide use of essential oils in pharmaceutical and food industry as antimicrobial agents, their use as wood preservatives has not been fully explored. In this study, 12 essential oils were screened in nutrient medium for their antifungal activity against 8 common mould, sapstain or decay fungi. Subsequently, one essential oil, eugenol was evaluated for decay resistance in an agar/wood ...
T Singh, C Chittenden


Ecotoxicity of furfurylated wood – Effect of leachate on aquatic bacteria
2008 - IRG/WP 08-50250
Environmental concern regarding the use of toxic preservatives such as CCA (chromated copper arsenate) has been put forward. In the EU, USA and Japan, CCA is now phased out for residential use and for use in water contact. Several ecotoxicological studies of wood treated with conventional preservatives were carried out in the late 1990s. In these studies it was concluded that the main impact is to...
A Pilgård, M Westin


Simulation and Investigation of Wood Degradation by Erosion Bacteria in Laboratory Experiments
2010 - IRG/WP 10-20431
A Microcosm experiment was successfully set up to establish, monitor and manipulate bacterial wood degradation under low oxygen conditions. Sound pine sticks were placed in waterlogged sediment from a heavily decayed pine pile foundation site in Amsterdam. The system was subject to different gassing treatment regimes in order to investigate the role of oxygen in the bacterial degradation process o...
J Gelbrich, E I Kretschmar, N Lamersdorf, H Militz


Isolation and evaluation of Lactobacillus brevis from chilli waste for potential use as a wood preservative
2011 - IRG/WP 11-10749
Lactic acid bacteria were isolated from chilli waste and evaluated for their ability to arrest wood rotting basidiomycetes. In previous work a quick screening method using 96 well plates and measuring absorbance to determine fungal growth was developed specifically to investigate the efficacy of isolated bacteria against wood decay fungi. Using this method, one bacterium (isolate C11) was identif...
D O’Callahan, T Singh, I R McDonald


Measuring fungal cellulolytic enzyme activity in degrading wood
2011 - IRG/WP 11-10753
An enzyme assay which measures enzyme activity directly from wood will assist in the fundamental understanding of the enzyme components of the decay mechanism of rot fungi and any changes in the presence of wood protectants. At present, to measure fungal carbohydrate degrading enzyme activity in the presence of wood, two methods are used: either 1) the enzyme is measured in the growth media contai...
S M Duncan, J S Schilling


Preliminary evaluation of degradation patterns in wood samples from the Yenikapı shipwrecks
2013 - IRG/WP 13-10798
The uncovering of several shipwrecks during the excavation of the Marmaray Rail Tube Tunnel Project in Istanbul provides an important opportunity to evaluate waterlogged woods buried for centuries. Identification of these woods showed that a variety of species was used in the ships. One shipwreck (a galley) from the Yenikapı Marmaray was examined using electron microscopy. The micrographs rev...
C Köse, A M Taylor


What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace


Evaluating the role of Actinobacteria in the gut of wood-feeding termites (Reticulitermes spp.)
2017 - IRG/WP 17-10880
Nitrogen has been shown to be a limiting nutrient across a range of xylophagous insects. These insects often rely on symbiotic microorganisms in the gut for nitrogen acquisition, via fixation of atmospheric nitrogen or break down of other available nitrogenous substances. In phylogenetically lower, wood-feeding termites, the role of nitrogen fixing bacteria has been well studied. However, there is...
Evaluating the role of Actinobacteria in the gut of wood-feeding termites (Reticulitermes spp.)


Analysis of hindgut bacterial phyla frequency and diversity in subterranean termites exposed to chitosan-treated wood
2017 - IRG/WP 17-10882
The termite hindgut contains a microbial community that symbiotically aids in digestion of lignocellulosic materials. For better understanding of the dynamics of the bacteria-termite relationship, a species survey of bacterial hindgut microbes in subterranean termites (Reticulitermes flavipes: Kollar) collected from Louisville, Mississippi was performed after exposure to chitosan-treated and contr...
O Raji, J D Tang, T Telmadarrehei, D Jeremic


Imports of African logs, do they harbor wood-decaying alien fungi and bacteria that could become a potential threat at temperate latitudes? – First results
2018 - IRG/WP 18-10904
In this project we investigate the potential threat of alien wood-decaying fungal and bacterial species imported to Germany with logs from African countries. In a first approach we analysed fungal species on imported logs by identification of fruit bodies by DNA sequencing of the internal transcribed spacer (ITS) regions ITS1 and ITS2. First results revealed the existence of at least one alien fun...
J Trautner, M Höpken, E Melcher


The Impact of Cryopreservation Techniques and of Water Quality on the Wood Degrading Ability of Fungal Strains: Recommendations for Maintenance and Culturing Procedures
2018 - IRG/WP 18-20636
In order to establish an optimal cryopreservation method, strains of Basidiomycete and Ascomycete fungi, which are among the most frequently used for wood durability testing, were evaluated using three cryopreservation procedures at -80°C. The main objective was to prevent decrease of the fungal virulence that may occur due to repeated routine sub-culturing procedures and to optimize the reactiva...
A Stum, M Montibus, I le Bayon, M Kutnik


Danish wood preservatives approval system with special focus on assessment of the environmental risks associated with industrial wood preservatives
2001 - IRG/WP 01-50166-01
The following is a description of the procedure used by the Danish Environmental Protection Agency to assess the environmental risks associated with preservatives used in the pressure impregnation of wood. The risk assessment covers issues considered to be of significance for the environment and which are adequately documented so as to allow an assessment. Such issues are persistence and mobility ...
J Larsen


Data sheet on wood-boring insects. Apate monachus Fabricius. 2. Position systématique, nomenclature, identification et distribution - Espèces végétales attaquée
1981 - IRG/WP 1105
R L A Damoiseau


Confocal laser scanning microscopy of a novel decay in preservative treated radiata pine in wet acidic soils
1997 - IRG/WP 97-10215
Light microscopy of radiata pine (Pinus radiata D. Don) field test stakes (20x20x500mm3) exposed in wet acidic (pH 3-4) soil for 12 - 24 months showed predominance of an unusual type of decay characte-rised by tunnelling attack of wood cell walls. After two years decay was moderate to severe in wood treated to ground contact CCA specifications and also equivalent retentions of creosote, and a numb...
R N Wakeling, Ying Xiao, A P Singh


Effect of acetylation on decay resistance of wood against brown-rot, white-rot and soft-rot fungi
1989 - IRG/WP 3540
Effect of acetylation on decay resistance of wood was investigated using wood blocks of Cryptomeria japonica, Pinus densiflora, Albizia falcata and Fagus crenata. Blocks were treated with uncatalyzed acetic anhydride for different lengths of time and exposed to Tyromyces palustris, Serpula lacrymans, Coriolus versicolor and unsterilized soil. The action of OH-radical on acetylated wood was also ex...
M Takahashi, Y Imamura, M Tanahashi


The IRG..Chanelling information and ideas into the mainstream of wood preservation technology
1985 - IRG/WP 5241
IRG Secretariat


Wood preservation in Poland
2004 - IRG/WP 04-30362
Dynamic growth of market demand for wooden elements and articles, generated in Poland increase of interest in industrial preservation. Today, Poland is a substantial producer and exporter of wood made products. Majority of exported wood - approximately 70% - is scotch pine (Pinus silvestris L.), which, due to its natural durability, requires preservation....
A Kundzewicz


Wood preservatives: Field tests out of ground contact. Brief survey of principles and methodology
1976 - IRG/WP 269
This paper contains the following spots: 1.: The general need for field tests. 2.: Interests and limits of field tests in ground contact. 3.: Various methods in use for out-of-ground contact field tests. 4.: Fungal cellar tests are they an alternative to above-ground decay exposure tests? 5.: Conclusions....
M Fougerousse


Management of the wood and additives wastes in the wood processing industries: Problematics and technical answers review
1996 - IRG/WP 96-50073
Management pathways for pure wood subproducts are well known and used; but as soon as additives like preservatives, glues, varnishes or coatings are present within the wood wastes, their disposal or valorization becomes more tricky. The different kinds of mixed wood wastes of the wood processing industries, from the sawmill to the furniture manufacture, are identified herewith and their diversity ...
S Mouras, G Labat, G Deroubaix


Biological screening assays of wood samples treated with creosote plus chemical additives exposed to Limnoria tripunctata
1980 - IRG/WP 408
Laboratory methods for exposure of treated wood coupons to Limnoria tripunctata are described. Chemical additions to creosote were screened using this method. Three pesticides, Endrin, Kepone, and Malathion proved particularly effective. The addition of varying percentages of naphthalene to creosote using several treatment methods are currently being assayed. Results to date show that the coupons ...
B R Richards, D A Webb


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