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Screening of lignin peroxidase from white-rot fungi
1998 - IRG/WP 98-10274
Since lignin peroxidase was first found in the ligninolytic culture of Phanerochaete chrysosporium, it has been regarded as an enzyme responsible for the lignin biodegradation and studied comprehensively. As the results of these studies its unique characteristics have been revealed. On the other hand, the features of lignin peroxidase produced by other white-rot fungi have not been studied so much...
I Momohara


The dry rot fungus Serpula lacrymans, its growth and damaging of wood
2004 - IRG/WP 04-10511
The dry rot fungus (Serpula lacrymans) is one of the most dangerous wood rotting fungi, especially in the built environment. In our mycological laboratory more experiments with this fungus have been carried out aimed at:- its growth under stable and variable climatic conditions, - its possibility to attack either natural wood of different species and also modified wood primary pre-treated with ...
L Reinprecht


Novel observations on the micromorphology of soft rot attack of wood
1996 - IRG/WP 96-10176
Electron microscopic examinations of decaying Pinus radiata horticultural posts which had been treated with CCA preservative prior to being placed in service about nine years ago showed the presence of soft rot fungi and tunnelling bacteria. Some novel observations on the micromorphology of the soft-rotted areas of tracheid walls are described here. The micromorphology of soft-rotted areas was hig...
A P Singh, R N Wakeling


Soft rot studies on CCA treated eucalypt power transmission poles
1981 - IRG/WP 1132
Initial results found for sixty CCA treated eucalypt poles, from a soft-rot survey are discussed. Retentions of CCA within above and below ground pole samples were variable. The variation was attributed mostly to biodegradation and effect of soil environment, rather than leaching. Bacterial decay was found in many poles and was severe in some cases. Although found in the presence of soft-rot decay...
L E Leightley


Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch


Biosynthesis of ß-Glucan microfibrils by cellular fractions from brown-rot fungus Postia placenta (MAD-698 and ME-20) and white-rot fungus Schizophyllum commune (MAD-619)
1993 - IRG/WP 93-10025
In this study, we compared the brown rot fungus Postia placenta (MAD-698 and ME-20) with the white rot fungus Schizophyllum commune (MAD-619) to determine the location and distribution of glucan synthetase. We also measured the soluble protein content in subcellular fractions obtained by differential centrifugation MAD-698 is a degradative isolate, but ME-20 and MAD-619 do not produce significant ...
S C Croan, T L Highley


An electron spin resonance study of manganese changes in wood decayed by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1359
Electron spin resonance (ESR) spectrometry was used to examine wood decay by the brown-rot fungus, Postia placenta. Wood slivers of Douglas-fir, white fir, redwood, sweetgum and yellow poplar were incubated for 4 weeks in custom-made quartz ESR tubes with or without Postia placenta. In all wood species without fungus, a weak partially resolved signal (about g=2, presumably due to manganese) was de...
B Illman, D C Meinholtz, T L Highley


Occurrence, prevention and repair of Dry Rot
1990 - IRG/WP 1439
Information about the frequency, detection, identification and repair of Dry Rot attacks (Serpula lacrymans) is summarized from 13 European countries. Based on 28 completed and returned questionnaires it appears that Dry Rot attacks are recognized in all countries participating, and that there is an appreciable similarity in the frequency of attacks and methods of detection and identification. How...
A P Koch


Termite and decay resistance of particleboard composed of white cypress pine and radiata pine
1997 - IRG/WP 97-10200
Phenol-formaldehyde bonded particleboard was manufactured from blends of non-durable radiate pine (P. radiate) and heartwood of the naturally durable species, white cypress pine (C. glaucophylla). Board specimens were subjected to bioassays using two termite species, M. darwiniensis and C. acinaciformis, and the basidiomycete fungi, C. puteana and P. ostreatus, and the durability of specimens was ...
P D Evans, J W Creffield, J S G Conroy, S C Barry


The effect of hemicellulose degradation on the mechanical properties of wood during brown rot decay
2001 - IRG/WP 01-20219
Incipient decay of wood by brown rot fungi causes measurable strength losses in wood before measurable weight loss occurs. Previous studies have shown that the high levels of strength loss that occur during incipient brown rot decay may be related to loss in hemicellulose. This paper investigates the effect of decay on hemicellulose composition and the relationship to the mechanical properties of ...
S F Curling, C A Clausen, J E Winandy


Variation in infection rates of blue-stain, mould and white rot tropical fungi on mixed light Malaysian woods
2000 - IRG/WP 00-10334
The modified 3-week FRIM laboratory method for screening of anti-sapstain formulations against three representative tropical fungi causing blue-stain (Botryodiplodia theobromae), mould (Paecilomyces variotii) and white rot (Schizophyllum commune) infection of sapwood species was used to examine the relative resistance of the sapwood of eight mixed light Malaysian woods, Scots pine (Pinus sylvestri...
A H H Wong, S Ahmad


Evaluation of fungal remediation of creosote treated wood
1998 - IRG/WP 98-50101-25
Biotechnological remediation of creosote treated wood may be of interest in connection with novel recycling processes. White rot fungi and/or their ligninolytic enzyme systems are supposed to be valuable tools for such processes. This paper reports about results achieved when creosoted wood was treated in solid substrate fermentation with selected white rot fungi after different extraction procedu...
K Messner, S Böhmer


Preliminary studies of the decay mechanism of some brown-rot fungi
1989 - IRG/WP 1402
The importance of the enzymatic degradation of hemicellulose and cellulose by brown-rot fungi is still under discussion. Endo-ß-1,4-xylanase and endoglucanase activities of Coniophora puteana and Poria placenta cultures were measured by the increase in reducing groups. Enzymes were produced in liquid and solid sawdust based culture media. Enzyme activities were measured in two, four and eight wee...
A-C Ritschkoff, H Viitanen


Soft rot decay of Belian (Eusideroxylon zwageri) wood
1995 - IRG/WP 95-10119
The heartwood of Belian (Eusideroxylon zwageri), an East Malaysian timber species, is naturally durable due to its certain unique anatomical features and high content of extractives. The timber can tolerate years of exposure to hazardous conditions in ground contact and other situations without any significant loss in its strength. A few Belian transmission poles sampled from one locality in Saraw...
A H H Wong, A P Singh


Soft rot - Soil burial - Tests. Influence of the water content of the soil on wood decay
1972 - IRG/WP 212
The decay of wood samples buried in the soil is influenced by various factors. Decisive are the water content, the content of organic substances, pH-value content of mineral nutritive substances etc. of the soil. For the tests, carried out by the EMPA until now, it was always started with a water content of 35%, refered to the dry weight of the EMPA-soil = 75% of its water capacity. In order to in...
O Wälchli


Decay resistance of Siberian larch wood against brown rot fungi - Part 2. The effect of genetic variation
1998 - IRG/WP 98-10287
Wood natural decay resistance is mainly restricted in the heartwood part of tree and based on wood microstructure and chemical composition. However, the variation of decay resistant in different wood species is vide and the effect of genotype on decay resistance of Siberian larch wood grown in Finland is not known. The aim of this work was to find out the variation on decay resistance of Siberian ...
H Viitanen, L Paajanen, T Nikkanen, P Velling


Soluble nutrient content in wood and its susceptibility to fungal discoloration and decay in above ground and ground tests
2000 - IRG/WP 00-10336
The objective of this comprehensive study was to reveal the impact of soluble nutrients in wood on its susceptibility to fungal discoloration and decay in various tests, which is important for the test methodology and standardisation. Seven tests were carried out including pure culture above and ground contact tests, above ground field test and laboratory ground contact tests. The samples containi...
O M Caballero, N Terziev


Evaluation of the pulsed-current resistance meter (Shigometer®) for determination of the internal condition of utility poles
1979 - IRG/WP 2128
A number of Telecom poles, which had been removed from service to be replaced by a radio link, were inspected using an electrical resistance meter (Shigometer®). These poles were creosoted, non-durable Victorian species in service in New South Wales. Six of these poles, chosen to represent various internal conditions typical of the range of the population tested, are discussed here. Data, from tr...
J D Thornton, W G Seaman, M McKiterick


Generation of hydroxyl radical by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1360
In an electron spin resonance (ESR) survey of various liquid cultures and wood slivers innoculated with the brown-rot fungus, Postia placenta, the spin trap dimethyl-l-pyrroline N-oxide (DMPO) was used to detect the presence of the hydroxyl radical. The ESR spectra for the paramagnetic DMPO- hydroxyl radical adduct was observed in (1) nitrogen-limited, liquid cultures having 1.0% glucose or 0.1% c...
B Illman, D C Meinholtz, T L Highley


Soft rot decay in acetylated wood. Chemical and anatomical changes in decayed wood
2002 - IRG/WP 02-40231
Acetylated Beech (Fagus sylvatica) and Scots Pine (Pinus sylvestris) wood were studied in soil beds under laboratory conditions for longer periods. High mass losses and dynamic MOE losses were measured in non-acetylated wood and at lower weight percent gains (WPG). Rapid losses of lignin, holocellulose and ?-cellulose occurred in non-acetylated beech wood and no losses were detected at the highest...
B Mohebby, H Militz


Accelerated laboratory testing of preservatives on 13 North American wood species
1999 - IRG/WP 99-30201
The ability of selected water and solvent based biocides to protect wood against fungal attack was evaluated on 13 North American wood species using 7 decay fungi in a modified soil block test. Most preservatives were capable of providing protection at their recommended above ground and soil contact use levels. Many decay fungi caused substantial weight losses on blocks treated with chlorothalonil...
J J Morrell, C M Freitag


Fenton's reagent as a modification tool in brown-rot decay
1996 - IRG/WP 96-10155
A biomimetic approach was used to clarify the role and importance of the Fenton-type reaction in the carbohydrate degradation by brown-rot fungi. Spruce sawdust and microcrystalline cellulose were modified in the H2O2/Fe(II) treatment. The degree of hydrolysis of the pretreated spruce sawdust was clearly increased with the complete cellulase (Econase), purified endoglucanase from Trichoderma reese...
M Rättö, A-C Ritschkoff, J Buchert, L Viikari


Preservative effect of cellulose insulation material against some mould fungi and brown rot fungus Coniophora puteana in pine sapwood
1991 - IRG/WP 1484
The influence of mineral wool and loose-fill cellulose wool on biodeterioration of pine sapwood was studied. The test fungi were mould fungi Aspergillus versicolor, Cladosporium sphaerospermum, Penicillium sp. and Aureobasidium pullullans and brown rot fungus Coniophora puteana. Cellulose wool inhibited the growth of mould fungi on the face of wood contacted with insulation material at RH of 97 an...
H Viitanen


Biochemical relationships between biodegradation of cellulose and formation of oxalic acid in brown-rot wood decay
1991 - IRG/WP 1472
Non-enzymic hydrolysis of cellulose with low concentrations of oxalic acid was examined. The incubation of pine wood pulp with 1% oxalic acid (pH 1.3) at 35°C for 4 weeks reduced the original viscosity to 60%. Reducing sugars were liberated from various cellulosic samples by the oxalic acid treatment. However, crystallinities of cellulose in those samples did not change before and after the treat...
M Shimada, Y Akamatsu, A Ohta, M Takahashi


Potential toxicants for controlling soft rot in preservative treated hardwoods. Part 1: Laboratory screening tests using a filter paper technique
1977 - IRG/WP 290
Thirty-one miscellaneous formulations have been screened using a filter paper assay technique, for their efficacy in controlling the growth of Chaetomium globosum, Cephalosporium acremonium, and a mixed inoculum of finely ground soft-rotted wood obtained from preservative treated transmission poles. The formulations consisted of various organic compounds and inorganic preparations made from differ...
H Greaves


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