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Production of monoclonal antibodies to fungal metabolites
1986 - IRG/WP 1306
The role of fungal extracellular enzymes in wood biodegradation is incompletely understood. Our lab is beginning a project utilizing monoclonal antibodies to characterize extracellular metabolites of the brown rot fungus Poria placenta Fr. (Cooke). Monoclonal antibody technology takes advantage of the ability of antibody secreting spleen cells from immunized mice to fuse in the presence of polyeth...
J Jellison, B Goodell


PXTS; A Metal Free Oligomer Wood Preserving System - A Summary of Data To Date
2004 - IRG/WP 04-30350
The world is continually looking for new wood preservative technology, especially to address environmental concerns. Recent changes in the USA have limited the use of arsenical containing formulations to industrial use through a negotiated voluntary cancellation of most residential label uses. PXTS (Polymeric Alkylphenol Polysulfide) offers a brand new technology that is an all organic system, lik...
M H Freeman, D D Nicholas, D Renz, R Buff


The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson


Influence of the nature of the substrate and the method of inoculation on the degradative activity of soft rot fungi
1998 - IRG/WP 98-20133
The European Standard (ENV-807) determines the threshold of effectiveness of wood preservatives against the action of soft rot Ascomycetes fungi. Its application in the treatment of wood used in the hazard class 4 does not always offer repeatable results, which could be expected in an experimental norm. For this reason, in this paper, we have evaluated the diverse variables that might influence t...
M T De Troya, A M Navarrete, F Rubio, J Jiménez, D Muñoz-Mingarro, C Rodríguez-Borrajo, F Llinares, M Yuste


The influence of cement and calcium compounds on the performance of CCA preservatives
1983 - IRG/WP 3221
The influence of cement and calcium compounds on the durability of untreated and CCA treated wood is considered. Calcium compounds were found to reduce the toxicity of a CCA preservative to a soft rot fungus at copper to calcium ratios of 1:1 and 1:10 using a cellulose filter paper technique. Further studies are outlined and some possible mechanisms by which cement and calcium compounds may affect...
R J Murphy


A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


The role of oxalic acid in short fiber formation by the brown-rot fungus Postia placenta
1993 - IRG/WP 93-10028
The mechanism by which brown-rot fungi depolymerize wood and cellulose remains a perplexing problem. Current evidence favors oxidation by low-molecular weight, non-enzymatic fungal metabolites. During attack of cotton cellulose by Postia placenta MAD-698, the degree of polymerization (DP) decreases to limit of degree of polymerization (LODP) over a period of 5-6 weeks with relatively low weight lo...
F Green III, J M Hackney, C A Clausen, M J Larsen, T L Highley


Micromorphology of Bamboo Fibers Degraded by Brown-Rot Fungus Gloeophyllum trabeum
2006 - IRG/WP 06-10576
The decay pattern of bamboo by brown-rot fungus was examined. In addition, the influence of polylaminate structures in bamboo fibers on the restriction of fungal decay was also investigated. The weight loss of bamboo Phyllostachys puberscens by the brown-rot fungus Gloeophyllum trabeum after 16 week incubation was about 25%. Parenchyma cells were severely degraded. Microscopical studies showed tha...
Kwang Ho Lee, Chang Hyun Cho, Yoon Soo Kim


Laboratory Evaluation of Soft Rot Resistance of Non-Durable Lesser-Known Malaysian Hardwoods
2006 - IRG/WP 06-10582
Resistance of six non-durable lesser-known Malaysian hardwoods, Pulai (Alstonia spp.), Rubberwood (Hevea brasiliensis), Ludai (Sapium spp.), Jelutong (Dyera costulata), Gaham Badak (Blumeodendron tokbrai) and Kayu arang (Diospyros spp.) compared with temperate Scots pine sapwood (Pinus sylvestris), were evaluated using the unsterile soil burial (mixed with Chaetomium globosum) versus vermiculite-b...
A H H Wong


Molecular characterization of M’jej, decaying agent of cedar forests in Morocco
2006 - IRG/WP 06-10593
Cedar wood is well appreciated since thousand years even though its economical importance is limited because of its world distribution restricted to some Mediterranean countries and Himalaya. The most important species, Cedrus atlantica have its biggest population in Morocco (130 000 ha) where it is submitted to fungal diseases. Among them, the locally named “M’jej” reduced notably saw mile ...
A Zaremski, S Bakkali-Yakhlef, C Chaintreuil, Y Abbas, Y Prin, M Abourouh, M Ducousso


Resistance of pine and spruce heartwood against decay - The effect of wood chemical composition and coating with water-borne wood oil product
2006 - IRG/WP 06-10597
Natural durability of wood has been widely studied, but the combination of the natural durability and different treatments has not been the focus of many studies. The durability of wooden products is mainly based on the water permeability and the resistance against organisms. In this study, the water absorption and decay resistance of sapwood and heartwood of Scots pine and Norway spruce were exam...
H Viitanen, S Metsä-Kortelainen, T Laakso


Manual for inspection of waterborne preservative treated timber products
2006 - IRG/WP 06-20322
This paper deals with inspection procedures of waterborne preservative treated timber products. A research study supported by past experience, knowledge and training on relevant issues and consultation of relevant bulletins and standards revealed a concise widespread guideline for the inspection of waterborne preservative treated timber products. Through this paper different types of templates on ...
A K Lahiry


Biological resistance of didecyl dimethyl ammonium tetrafluoroborate (DBF)-treated wood in soil-bed and Basidiomycetes tests
2006 - IRG/WP 06-30393
This study evaluated the decay resistance of treated wood with a new quaternary ammonia compound, didecyl dimethyl ammonium tetrafluoroborate (DBF) in laboratory soil bed and Basidiomycetes tests. Treated sugi sapwood specimens with DBF at various concentration levels were first subjected to soil bed tests using two types of soil. The specimens subjected to soil bed tests were then used for Basidi...
S N Kartal, C Brischke, A O Rapp, Y Imamura


Phenol oxidase activity and one-electron oxidation activity in wood degradation by soft-rot deuteromycetes
2007 - IRG/WP 07-10615
Wood degradation, one-electron oxidation activity as assayed by ethylene generation from 2-keto-4-thiomethylbutyric acid (KTBA), and phenol oxidase activity were measured in cultures of six deuteromyce fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing...
H Tanaka, M Yamakawa, S Itakura, A Enoki


Gypsum effects on ‘dry rot’ wood degradation as a function of environment
2007 - IRG/WP 07-10624
‘Dry rot’ fungi are a unique group of brown rot fungi that can degrade wood away from ground contact where other fungi fail to colonize. Successfully occupying this niche is partially due to efficient water and nutrient transport, but mobilizing elements, notably calcium (Ca) and iron (Fe), from adjacent building materials has also been implicated in their success. Here we report a series of t...
J Schilling, J Jellison


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


Detection of Anti-Fungal Sapwood Extractives in Non-Durable Scots Pine (Pinus sylvestris), Rubberwood (Hevea brasiliensis) and Jelutong (Dyera costulata)
2007 - IRG/WP 07-10634
A general laboratory bioassay method of Woodward and Pearce (1985) was adopted to detect anti-fungal activity of sapwood or heartwood extractives of 5 Malaysian hardwoods [dark red meranti heartwood (Shorea spp.), red balau heartwood (Shorea spp.), kulim heartwood (Scorodocarpus borneensis), jelutong sapwood (Dyera costulata) and rubberwood sapwood (Hevea brasiliensis), including the temperate Pin...
A H H Wong, R B Pearce


Soft Rot Determines Service Life of L-joints with Low Borate Loading
2008 - IRG/WP 08-30470
As part of a research program on the potential use of borates by the Canadian wood products industry, Forintek set up an L-joint test of untreated and borate-diffusion treated hem-fir (western hemlock and amabilis fir) in 1990. The treated material had a low initial retention of only 0.2% boric acid equivalent and analysis after 5 years revealed no detectable borate close to the tenon. Nev...
P I Morris, J Wang, J K Ingram


Effect of a biological treatment on below ground decay of Douglas-fir pole sections
2008 - IRG/WP 08-40433
The use of exogenous sugars to accelerate microbial growth and eventually limit available oxygen in soil surrounding wood in soil contact was investigated on untreated Douglas-fir poles exposed over a 3 year period in Western Oregon. Isolation frequencies differed markedly between treated and untreated poles and the treatment did appear to shift the frequency of some basidiomycetes. The treatment ...
C Freitag and J J Morrell


Effect of different ASAs (alkenyl succinic anhydrides) on the treatment of biological protection of wood in use class 4
2008 - IRG/WP 08-40440
The alkenyl suscinic anhydrides (ASAs) used in this work are adducts resulting from the maleinization reaction of fatty acid alkyl esters. Various products with different alkyl groups were synthesized from rapeseed (Brassica napus) oil esters, rich in oleic acid. The liquids obtained showed a viscosity similar to that of vegetable oils and a brown color. When applied into wood, a thermal treatment...
C Vaca-Garcia, O Pignolet


Serpula lacrymans – calcium, iron, and foundering wooden boats
2009 - IRG/WP 09-10691
Serpula lacrymans is one of the most destructive wood-degrading brown rot fungi in temperate environments. Its virulence has often been linked to its ability to grow over non-woody materials and extract calcium (Ca) or iron (Fe) to promote wood degradation in buildings. This fungus has also been a severe problem in historic wooden warships and in modern wooden vessels, sometimes leading to founder...
J S Schilling, S M Duncan


Effects of cold treatment on wood destroying fungi important in cultural heritage
2009 - IRG/WP 09-10706
The dry rot fungus Serpula lacrymans (Wulfen:Fr.) Schroeter is notorious in building environments for its vitality, destructive potential, and the huge costs associated with remediation of damage caused to wood of cultural heritage. Due to the potentially adverse of effects of chemical treatment methods such as have been attempted in the past, more benign physical methods of treatment, e.g. heat t...
M Lüdicke, W Unger, G Binker


Degradation of wood veneers by Fenton’s reagents: effects of low molecular weight phenolic compounds on hydrogen peroxide decay and tensile strength loss
2009 - IRG/WP 09-20400
Pine wood (Pinus sylvestris) veneers strips were incubated in acetate buffer containing hydrogen peroxide and iron to mimic mechanisms of brown rot decay and assess the degradation of cellulose through analysis of the tensile properties of the decayed wood. The tensile properties of thin wood strips treated with Fenton system reagents or precursors were determined and correlated to weight loss as ...
Yanjun Xie, R Well, Zefang Xiao, B Goodell, J Jellison, H Militz, C Mai


Wart Morphology can Distinguish White Cypress Pine from the Less Durable Species, Black Cypress Pine
2009 - IRG/WP 09-20406
White cypress pine (Callitris glaucophylla) wood is durable enough to be used outdoors, but occasionally there are reports of its premature failure in ground contact, which may be due to its substitution by the less durable species, black cypress pine (C. endlicheri). It has been difficult to prove this, however, because the woods of both species are very similar in structure and cannot be separat...
R Heady, R Cunningham, P Evans


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