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Effect of Volatiles from Trichoderma species on the regulation of protein synthesis in Serpula lacrymans isolates
2002 - IRG/WP 02-10440
The growth of wood decay fungi has previously been shown to be inhibited by volatile organic compounds (VOCs) from selected Trichoderma isolates. No mechanism of action has however been established for such inhibition. This paper reports the effects of VOCs produced by Trichoderma pseudokoningii, T. viride and T. aureoviride on the growth of four dry rot isolates and corresponding protein synthesi...
S Humphris, R Wheatley, E Buultjens, A Bruce


Detection of dry rot by air analysis
1992 - IRG/WP 92-2399
Detection of dry rot (Serpula lacrymans) at an early stage appears to be very difficult in practice. Also control inspections in buildings, after remedial treatment of dry rot, have a limited accuracy. The use of trained dogs in Denmark initiated the idea for this research on the possible use of air analysis as a detection method. The Centre for Timber Research-TNO (TNO-CHT) and the Institute for ...
P Esser, A C Tas


Synergistic effect of boron on Streptomyces rimosus metabolites in preventing conidial germination of sapstain and mold fungi
1992 - IRG/WP 92-1565
We evaluated the synergistic effect of boron (4% BAE solution of Tim-Bor or 4% boric acid) on Streptomyces rimosus metabolites in preventing spore germination of sapstain and mold fungi using plate bioassay, Southern yellow pine and sweetgum block tests, and green pine log sections: sapstain -- Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans; mold fungi -- Aspergillus nig...
S C Croan, T L Highley


Biological control with Trichoderma harzianum in relation to the formation for spores the production of soluble metabolites
1994 - IRG/WP 94-10073
The amount of spores produced by three strains of Trichoderma harzianum on the aerial mycelium of agar cultures and in shake cultures, respectively, correlated with the inhibition zones exerted against Phanerochaete chrysosporium in an agar diffusion test. The amount of soluble antifungal metabolites as well as the protein content also correlated with the inhibition zones and the amount of spores ...
J Bürgel, E Horvath, J Haschka, K Messner


Volatile borates in the treatment of wood and wood based panel products against subterranean termites
1995 - IRG/WP 95-30094
Blocks of pine solid wood, oriented strand board and plywood were treated with trimethyl borate by vapour treatment. It was meant to obtain either a full impregnation of the specimens or a shell treatment of the outer three millimetres. The efficacy of the treatments against subterranean termites (Reticulitermes lucifugus) was evaluated using an European Standard method....
L Nunes, D J Dickinson, R J Murphy


Inhibition of termite feeding by fungal siderophores
1992 - IRG/WP 92-1558
Siderophores are iron-chelating extracellular fungal metabolites which may be involved in initiating wood decay. A purified siderophore extract isolated from the brown-rot decay fungus Gloeophyllum trabeum (Pers. ex Fr.) Murr. (Basidiomycetes: Polyporaceae) was found to deter feeding by Formosan subterranean termites, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). This fungus has prev...
J K Grace, B Goodell, W E Jones, V Chandhoke, J Jellison


Treatment of wood-based panel products with volatile borate
1990 - IRG/WP 3616
The paper presents recent developments in the use of volatile borate esters for the preservative treatment of wood based board materials. Several advances on previous reports are discussed. In laboratory studies, treatment times of approximately ten minutes at 20°C on boards at equilibrium moisture content provided full penetration and retentions of 1% wt/wt boric acid. Biological tests have been...
P Turner, R J Murphy, D J Dickinson


Analysis of volatile emissions as an aid in the diagnosis of dry rot
1992 - IRG/WP 92-2393
The dry rot fungus, Serpula lacrymans was grown in pure culture on malt extract and on sapwood of pine. The volatile compounds emitted from the cultures were determined by diffusion sampling on tubes filled with Tenax TA, thermal desorption and gas-chromatography-mass-spectrometry in order to find markers for attack of the fungus....
J Bjurman, J Kristensson


Immunolocalization of extracellular metabolites from Tyromyces palustris
1991 - IRG/WP 1491
Polyclonal antisera produced to extracellular metabolites from the brown-rot fungus Tyromyces palustris was used in immunogold TEM studies. Gold labelling was detected in the fungal cell wall and extracellular slime layer but little or sporadic labelling was noted within the cytoplasm of the fungal hyphae. Gold particles were also found within the wood cell wall of Pinus densiflora decayed by Tyro...
Yoon Soo Kim


Volatile losses of wood preservatives
1973 - IRG/WP 236
The purpose of the work we have undertaken so far has been to try to quantify the volatile losses which occur in treated timber, and to examine some of the factors which influence these losses. The preservative compound chosen for the initial part of the programme was the contact insecticide g-BHC. This was attractive for several reasons, not the least of which was that specific and sensitive anal...
J W W Morgan, D F Purslow


Controlling the sapstain fungus Ceratocystis coerulescens by metabolites obtained from Bjerkandera adusta and Talaromyces flavus
1993 - IRG/WP 93-10024
Sapstain causes severe damage to wood and wood products, posing a major economic problem for the wood industry. The purpose of this study was to determine if metabolites from Bjerkandera adusta and Talaromyces flavus would (1) decolorize stain in wood caused by Ceratocystis coerulescens and (2) prevent sapstain by Ceratocystis coerulescens. We studied the interaction of the sapstain fungus Ceratoc...
S C Croan, T L Highley


Protection of rubberwood with modified creosote
1998 - IRG/WP 98-30165
Creosote is a renowned wood preservative but has certain disadvantages like obnoxius odour and colour, with its unpaintability and bleeding from wood surface may create environmental hazards. An effort has been made to isolate chemical fractions from creosote to obtain clean odourless preservative formulations by subjecting to steam distillation (SVC). Rubberwood treated with SVC and tested for it...
H C Nagaveni, H S Ananthapadmanabha, G Vijayalakshmi, M N Sharma, K H Shankaranarayana


Volatile organic compounds (VOC) measured during drying of impregnated timber
2004 - IRG/WP 04-50216
Discussion about drying of impregnated timber has taken place in the Nordic countries under eighties and nineties and goes on nowadays. The drying increase the value of production and the customer gets fewer problems when handles the timber. This introduces a positive attitude to the impregnation as a process and its products. Beside the important information about the advantages of impregnated ti...
N Terziev, V Djokic


Antifungal properties of metabolites produced by Trichoderma isolates from sawdust media of edible fungi against wood decay fungi
1994 - IRG/WP 94-10051
Trichoderma isolated from a sawdust medium of Pholiota nameko produced filtrates which had antifungal activities to four wood decay fungi tested on the agar plates. Filtrates produced from another isolate, from a sawdust medium of Lentinus edodes, had antifungal activities only to the white rot fungi, Coriolus versicolor and Pycnoporus coccineus. These results did not agree with those from earlier...
S Doi, M Mori


Control of sapwood-inhabiting fungi by fractionated extracellular metabolites from Coniophora puteana
1991 - IRG/WP 1494
The objective of this study was to test the fractionated metabolites released by Coniophora puteana for their antagonistic activity against the sapstain fungi Ceratocystis coerulescens and Aureobasidum pullulans, and the molds Asperigillus niger and Penicillium spp. The acetone-soluble fraction obtained from the culture filtrate prepared from Coniophora puteana grown on 6% malt extract agar inhibi...
S C Croan, T L Highley


Assessment of the Inhibition of wood decay fungi by volatile organic compounds identified from Trichoderma spp.
1999 - IRG/WP 99-10302
Previous research identified five volatile organic compounds produced by Trichoderma spp. that may be inhibitory to wood decay fungi. The effects of four of these volatile organic compounds, 2-propanone, 2-methyl-1-butanol, heptanal and octanal were tested over a range of concentrations against four selected wood decay fungi. The fungi were incubated in malt extract broth under appropriate conditi...
S N Humphris, R E Wheatley, A Bruce, C Payne


Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison


Biological control of decay
1975 - IRG/WP 135
One approach to the biological control of decay in standing poles, live trees or seedlings is with microbial immunising commensals or IC.·These are microorganisms able to grow in the wood without damaging it and as a result of such growth to protect against certain types of decay. Scytalidium and Trichoderma spp have been tested in the field. The latter species have shown an establishment rate in...
J Ricard


Antagonistic effect of Trichoderma spp. against basidiospores
1993 - IRG/WP 93-10027
All screening of potential biocontrol agents of wood decay fungi have previously been used against basidiomycete mycelium. Similarly, experiments designed to evaluate the mechanisms involved in antagonism (soluble metabolites and volatile antibiotics) between biological control agents and target fungi have always been carried out on mycelial inoculum. Basidiospores are a primary source of infectio...
U Srinivasan, T L Highley, S C Croan, A Bruce


Analysis of volatile organic compounds (VOC's) from Trichoderma spp.: effect of media composition on VOC production and level of inhibition of wood decay fungi
1997 - IRG/WP 97-10207
Production of volatile organic compounds (VOC's) by two Trichoderma isolates grown on either malt extract or minimal media was examined and statistically assessed to identify which VOC's were predominantly produced on each of the two media types. The VOC's were captured onto chromatographic absorbent from above the cultures of the fungi before being analysed by integrate...
A Bruce, R E Wheatley, C Hackett, A Kundzewicz


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


Control of wood biodeterioration by fungal metabolites
1992 - IRG/WP 92-1527
Treating wood with different conventional preservative chemicals for protection and economic utilisation is well known. Tough some of these chemicals are toxic to skin, their use as wood preservative is unavoidable as they should be toxic to biodeteriorating organisms also. Recent advances in chemistry of natural products enabled the use of wood extractives as biocidal compounds against biodeterio...
H S Ananthapadmanabha, H C Nagaveni, V V Srinivasan


Environmental characterisation of retification process by-products (liquid and gaseous wastes)
2005 - IRG/WP 05-50224-10
In order to reduce environmental risks during the service life of the treated wood and to find new alternative developments on the durability of wood, some research and technology development have been made on thermal treatment. The retification process is one of these processes. The retification process induces chemical modification of the lignin and cellulosic components and modifies the intrins...
G Labat, E Bucket, S Legay, C Yriex, P Marchal, E Raphalen, M Vernois, R Guyonnet, H Besset, E Fredon), G Vilarem, L Rigal, C Raynaud


Free radical process controlled by manganese peroxidase and lipid-related metabolites produced by Ceriporiopsis subvermispora
2001 - IRG/WP 01-10412
Ceriporiopsis subvermispora, a specific lignin-degrading fungus produced free unsaturated fatty acids (USFAs) including 9,12-octadecaienoic asid, together with saturated fatty acids (SFAs) at an incipient stage of cultivation on wood meal cultures. In prolonged cultivation period after two weeks, the amount of intact fatty acids decreased with increasing in organic hydroperoxide and TBARS producti...
T Watanabe, M Enoki, S Sato, Y Honda, M Kuwahara, N Shirai, K Messner


Antifungal activity in metabolites from Streptomyces rimosus
1991 - IRG/WP 1495
The objective of this study was to evaluate the efficacy of antifungal metabolites from Streptomyces rimosus for controlling the growth of sapwood-inhabiting fungi: sapstain fungi - Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi - Aspergillus niger, Penicillium spp, and Trichoderma spp. Production of antifungal metabolites by Streptomyc...
S C Croan, T L Highley


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