Your search resulted in 131 documents. Displaying 25 entries per page.
Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison
Properties of cellulose degraded by the brown rot fungus, Postia placenta
1988 - IRG/WP 1350
To gain further understanding of the nature of the cellulose depolymerizing agent or agents of brown-rot fungi, brown-rotted cellulose was chemically and physically characterized. Various culture conditions, such as low nitrogen and elevated oxygen levels, did not induce degradation of cellulose by Postia placenta in liquid cultures. Therefore, brown-rotted cellulose for analysis was prepared by a...
T L Highley, R E Ibach, T K Kirk
Dimensional stabilization and decay resistance of wood treated with brown-rotted lignin and copper sulfate
1990 - IRG/WP 3608
The objective of this study was to evaluate the potential usefulness of brown-rotted lignin (BRL) as a dimensional stabilization and copper complexing agent for wood treatment. For dimensional stabilization, aqueous solutions of the lignin extract were combined with either copper sulfate, glyoxal or other additives. Anti-shrink efficiency (ASE) values as high as 42% were obtained with wood treated...
L Jin, D D Nicholas, T Schultz
Silicon tetrachloride: A potential wood preservative
1980 - IRG/WP 3133
In its present form, this paper is a preliminary draft of a paper that will eventually be submitted for publication in the Forest Products Journal. We are continuing the work on the effects of silicon tetrachloride on wood and expect to have additional data for the meeting in May 1980. Specifically we are measuring the penetration rate and depth of SiCl4, and we are chemically analyzing the treate...
C W Owens, W T Shortle, A L Shigo
Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse
The nature of osmiophilic particles and their distribution during different stages of brown and white rot decay
1983 - IRG/WP 1213
The distribution of osmiophilic particles during the course of brown and white rot decay was investigated by applying transmission electron microscopic (TEM) methods. It was found that it correlates with the brown and white rot pattern.The osmiophilic particles are produced by the fungus and are supposed to be wood rotting enzymes....
K Messner, H Stachelberger
Effect of crude tall oil, linseed oil and rapeseed oil on the growth of the decay fungi
2002 - IRG/WP 02-30299
The influence of crude tall oil, linseed oil and rapeseed oil on the growth of Coniophora puteana, Poria placenta and Coriolus versicolor was studied. The selected test oils were observed to have different effects on the the fungal growth. Crude tall oil inhibited the radial growth of all fungi. Rapeseed oil either accelerated or inhibited the growth of fungi depending on the type of fungus involv...
L Paajanen, A-C Ritschkoff
Screening wood preservatives: Comparison of the soil block, agar block and agar plate tests
1993 - IRG/WP 93-20001
Several test procedures have been developed over the years to screen potential biocides for their value as wood preservatives. Each test has inherent advantages and disadvantages. In this paper the relative merits of the soil block, agar block and agar plate tests are compared. Eight commercially available biocides encompassing inorganic and organic systems were tested against four basidiomycete d...
K J Archer, D D Nicholas, T Schultz
Effects of air-seasoning on fungal colonization and wood strength of Douglas fir poles
1987 - IRG/WP 1315
Air seasoning economically reduces the moisture content of Douglas fir poles before pressure treatment with preservatives. Advanced decay in poles in service has resulted when decay fungi (Basidiomycetes) colonized poles during air-seasoning and survived the treatment process. These problems have led to recommendations to severely limit this practice. To determine the role of these fungi in peeled...
J J Morrell, M E Corden, R D Graham, B L Kropp, P Przybylowicz, S M Smith, C A Sexton
The production of extracellular hydrogen peroxide by some brown-rot fungi
1990 - IRG/WP 1446
The role of hydrogen peroxide (H2O2) has been discussed in the degradation of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by detecting the oxidation of the chromogen 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horse radish peroxidase (HPR). ABTS and HPR were added to a solid wood based culture media. In this study ...
A-C Ritschkoff, L Paajanen, L Viikari
Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson
Cation analysis of wood degraded by white and brown rot fungi
1992 - IRG/WP 92-1552
Changes in the concentrations and composition of cations within wood colonized by the brown-rot fungi Postia placenta and Gloeophyllum trabeum and the white-rot fungi Trametes versicolor and Phanaerochaete chrysosporium were analyzed using plasma emission and atomic absorption spectrophotometry. In degraded poplar wood significant decreases in pH and electrical resistance readings were observed as...
J Jellison, K C Smith, W T Shortle
Effects of chemical pretreatment of Douglas-fir heartwood on efficacy of potential bioprotection agents
1990 - IRG/WP 1440
Biological protection against wood decay fungi represents an environmentally attractive alternative to the use of chemicals; however, the process of identifying suitable candidates and the conditions that ensure successful protection pose major challenges. One critical aspect of this problem is to identify organisms that can rapidly and uniformly colonize the wood. Wood contains low levels of nutr...
B Dawson-Andoh, J J Morrell
Co-operative studies on determining toxic values against wood-destroying basidiomycetes: Progress report to April 1990
1990 - IRG/WP 2357
This document reports progress on the co-operative study between nine laboratories, set up following the proposals contained in Document IPG/WP/2316 (1988). Results have been received from eight laboratories. Toxic values data have been established successfully for the test fungus Coniophora puteana with soil, malt agar and vermiculite methods and with the test fungi Gloeophyllum trabeum and Poria...
J K Carey, A F Bravery
Biological resistance of electrolessly plated wood (1). Preliminary report
1994 - IRG/WP 94-40024
Surface metal coating is considered to improve biological resistance of wood as some metal ions are fungitoxic. Six wood species (three softwoods: Cryptomeria japonica, Tsuga heterophylla and Larix spp; three hardwoods: Fagus crenata, Acer mono and Betula platyphylla) were electrolessly plated with nickel or copper, and those were served for laboratory evaluation of their resistance against decay ...
M Hasegawa, K Tsunoda, T Yoshimura
Enzyme immunoassay to detect Postia placenta in field tests: Comparison of plate ELISA with hydrophobic cloth and cotton dipstick
1991 - IRG/WP 2378
Standard indirect enzyme-linked immunosorbent assay in polystyrene 96-well plates was compared to hydrophobic polyester cloth and cotton dipstick for detection of wood-derived antigens from the brown-rot fungus Postia placenta. The ease of handling, larger surface area, and economics of the latter two adsorbents were surveyed for application as field tests for detection of early decay. At high ant...
C A Clausen
Method to determine the depth of penetration of the biologically active components of wood preservatives
1978 - IRG/WP 2108
A time-saving method for determining the depth of penetration of the biologically active components of wood preservatives is described. The test specimens were obtained by cutting thin slices from the wood either parallel or perpendicular to the treated surface. The slices were then exposed to fungal attack. A good correlation was found between the test results obtained by the modified German Stan...
H-P Sutter
Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales
Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley
The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales
Biological resistance of furfuryl alcohol-treated wood
1992 - IRG/WP 92-3703
Biological resistance of FFA (furfuryl alcohol)-treated wood was investigated in relation to the rise of ASE (anti-swelling efficiency) resulting from the treatment. Sapwood blocks of Japanese red pine, cedar and beech were vacuum-impregnated with various concentrations of aqueous FFA solutions to get the target levels of% (w/w) RL (resin loading). Air-dried blocks were kept in an oven at 120°C f...
J Y Ryu, Y Imamura, M Takahashi
Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley
Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell
Antagonism of Scytalidium lignicola against wood decay fungi
1989 - IRG/WP 1392
Antagonistic abilities of Scytalidium lignicola against white- and brown-rot wood decay fungi were evaluated. Scytalidium lignicola did not produce inhibition zones but overgrew the decay fungi on a malt-agar medium and in most cases killed them. Pretreatment of Douglas-fir and Southern pine blocks with Scytalidium lignicola prevented decay. Blocks that were heated or treated with propylene oxide ...
T L Highley
Production of monoclonal antibodies to fungal metabolites
1986 - IRG/WP 1306
The role of fungal extracellular enzymes in wood biodegradation is incompletely understood. Our lab is beginning a project utilizing monoclonal antibodies to characterize extracellular metabolites of the brown rot fungus Poria placenta Fr. (Cooke). Monoclonal antibody technology takes advantage of the ability of antibody secreting spleen cells from immunized mice to fuse in the presence of polyeth...
J Jellison, B Goodell