IRG Documents Database and Compendium


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Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner


Destruction of two tropical timbers by marine borers and micro-organisms in Goa waters (India)
1992 - IRG/WP 92-4176
The paper deals with the pattern of microbial attack in relation to marine borer damage of test panels of African rosewood and Brazilian jackwood exposed in Goa waters (Western India) for a period of seven months. The degradation of wood cell walls in both the timber samples occurred due to infestation of soft-rot fungi and tunnelling bacteria. African rosewood, which was severely damaged by tered...
L N Santhakumaran, A P Singh


Attempts to isolate tunnelling bacteria through physical separation from other bacteria by the use of cellophane
1992 - IRG/WP 92-1535
During attempts to isolate tunnelling bacteria in pure culture, physical separation from other bacteria was successfully achieved by the use of cellophane. Cellophane was used in liquid cultures as a substrate for tunnelling bacteria that were present in a mixture with other bacteria. By tunnelling into cellophane, TB were physically removed from contaminating bacteria which could then be removed ...
T Nilsson, G F Daniel


Tunnelling bacteria
1983 - IRG/WP 1186
The micromorphology of decay in wood caused by a certain group of single-cell bacteria, called tunnelling bacteria (TB), is described. TB are characterised by their tunnelling ability within the secondary cell walls of wood fibres. Pure cultures of TB have not yet been obtained although the typical decay patterns can be reproduced using mixed bacterial cultures. Some indications of lignin degradin...
T Nilsson, G F Daniel


Microbial decay in an extremely durable Malaysian hardwood Belian (Eusideroxylon zwageri) - an overview
1997 - IRG/WP 97-10216
The heartwood of Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods, and is therefore a choice timber for soilcontact use in the humid tropics such as untreated telecommunication poles, fence posts and railway sleepers. The high microbial resistance of the wood is evidenced by the mere superficial degradation observed in some pole samples after 20 yea...
A H H Wong, A P Singh


Bacterial degradation of Pinus radiata compression wood
1996 - IRG/WP 96-10153
An inspection of twelve year old, CCA-treated Pinus radiata wood from an industrial cooling tower showed extensive surface decay of wood. Electron microscopic examination of decaying compression wood indicated that compression wood cells were attacked exclusively by bacteria, which were of erosion and tunnelling types. As compared to the normal wood, compression wood appeared to be more resistance...
A P Singh, R N Wakeling


Hyphal tunnelling of belian (Eusideroxylon zwageri) wood cell walls
1996 - IRG/WP 96-10152
Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods. Visual inspections at the groundline of belian transmission poles from Sarawak, Malaysia, showed only surface decay of wood after 20 years in service. The cause of decay in belian was investigated by light and transmission electron microscopy. Light microscopy of transverse sections of surface tissue...
A P Singh, A H H Wong


Standard principles of testing termiticides: A discussion paper
1991 - IRG/WP 1502
Recent restrictions and banning of termiticides such as organochlorines in some countries has focussed attention on the need for new and novel compounds as termiticides. However, this poses problems for both wood preservative manufacturers and termitologists. Test procedures have to be devised to evaluate the new termiticides so that the tests are practical, encourage vigorous termite activity, an...
J R J French


Evidence for actinomycete degradation of wood cell walls
1990 - IRG/WP 1444
Several unique patterns of degradation occurring in wood cell walls have been observed in wooden stakes inserted in unsterile soil in the laboratory. Some of the patterns have also been observed in coniferous wood taken from forest floors. All the observed attack types occur within wood cell walls, mainly within the S2 layer. Attack is characterised by channels of varying diameter or small fusifor...
T Nilsson, G F Daniel, S L Bardage


Ingestion of wood-degrading micro-organisms
1991 - IRG/WP 4169
An ultrastructural study was carried out on the digestive tract contents of Limnoria lignorum, frass, gallery walls and surface structures of the animals. The purpose of the study was to determine whether wood-degrading bacteria, fungi and other microbes and/or wood degraded by these microbes were present in gut regions and therefore could provide indirectly a nutritional source for Limnoria. Both...
G F Daniel, S M Cragg, T Nilsson


Occurrence and importance of various types of fungal and bacterial decay in CCA-treated horticultural pine posts in New Zealand
1984 - IRG/WP 1234
A detailed microscopical examination has been carried out on samples taken from CCA-treated pine posts exposed in horticultural soils. The following decay types were observed: white rot, brown rot, soft rot, tunnelling bacteria, cavitation bacteria, and bacterial erosion. The occurrence and importance of the various decay types between different regions, and plots within a specific region, varied ...
T Nilsson


The effect of concrete embedment on CCA treated hardwood and softwood timbers
1985 - IRG/WP 3340
Small size stakes of Eucalyptus maculata and Pinus elliottii were treated with CCA to various retentions and embedded in concrete collars containing varying amounts of a fungicide additive. During the 30 month trial period stakes were removed after 12, 18, 24 and 30 months and assessed for residual strength. Stakes which showed significant strength reductions were microscopically examined for the ...
L E Leightley, G A Willoughby


The development of soft rot decay in bamboo fibres
1992 - IRG/WP 92-1572
The development of decay by soft rot fungus Chaetomium globosum was investigated in young and mature culms of bamboo Phyllostachys virideglaucescens. Soft rot decay was influenced both quantitatively and qualitatively by the differing levels of lignification in the material. Weight loss was significantly greater in the younger culm compared with the older, more heavily lignified culm. The distribu...
O Sulaiman, R J Murphy


Bacterial degradation of wood cell wall: A review of degradation patterns
1990 - IRG/WP 1460
Information from bacterial degradation studies of 60's and 70's was reviewed by Nilsson in 1982. The application of electron microscopy to this area in recent years has provided much useful information and has eliminated earlier scepticism among workers about the ability of bacteria to degrade lignified wood cell walls. Studies using transmission electron microscopy together with...
A P Singh, J A Butcher


A model for attack at a distance from the hyphae based on studies with the brown rot Coniophora puteana
1995 - IRG/WP 95-10104
In timber infested by brown rot fungi, a rapid loss in strength is attributed to production of hydroxyl radicals (HO·) at a distance from the hyphae. The immediate precursor is Fenton's reagent (Fe(II)/H2O2), but the pathways leading to Fe(II) and H2O2 have remained unclear. Cellobiose dehydrogenase, purified from cultures of Coniophora puteana, will couple oxidation of cellodextrins to ...
S M Hyde, P M Wood


Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell


Preservation of basidiomycete hyphae in ancient waterlogged wood materials
1992 - IRG/WP 92-1536
Studies on waterlogged archaeological wood show that basidiomycete hyphae may persist as long as 800 years. In two pine wood samples with Phellinus pini heartrot, one from the foremast of the ship Vasa and the other from a bulwark constructed in the first part of the 12th century, numerous resin covered hyphae were observed. Hyphae with clamp connections that were associated with brown and white r...
T Nilsson, G F Daniel


New Observations and Interpretation for Tunnelling Bacteria Decay
2006 - IRG/WP 06-10579
Decay micromorphology was studied systematically for diversely preservative treated Pinus radiata and Fagus sylvatica 20 x 20 x 500 mm stakes across 13 in-ground field test sites, during a 6.5 year exposure. Sites were selected to maximise occurrence of a diverse range of decay types. Micromorphology that suggested orientation of tunnelling bacteria with the cellulose microfibrils of the S2 and S1...
R Wakeling


Evidence for Basidiomycete Tunnelling in Pinus radiata and Fagus sylvatica
2006 - IRG/WP 06-10587
Decay micromorphology was studied systematically for diversely preservative treated Pinus radiata and Fagus sylvatica 20 x 20 x 500 mm stakes across 13 in-ground field test sites, during a 6.5 year exposure. Sites were selected to maximise occurrence of a diverse range of decay types. Presence of clamp connections in close associated with tunnelling coupled with macroscopic features similar to whi...
R Wakeling


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