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Disodium octaborate tetrahydrate, alone and in conjunction with a selected isolate of Trichoderma viride, reduces decay of fresh felled pine independent of the effect of weathering
1994 - IRG/WP 94-10054
Unpeeled billets of Corsican pine were treated with the following: 5% aqueous disodium octaborate tetrahydrate (DOT) solution; 5% DOT solution with an isolate of Trichoderma viride with a low susceptibility to borate; the Trichoderma viride isolate alone. Half of the billets in each treatment group were also placed in large-diameter plastic pipes to protect the billets from the weather. All three ...
M W Schoeman, D J Dickinson, J F Webber


Differential susceptibility of living and dead timber to colonisation by sapstain and mould fungi
1998 - IRG/WP 98-10288
Field studies have revealed that when timber is irradiated (i.e. killed) it is more susceptible to colonisation by mould fungi than sapstain fungi. By comparison, freshly sawn timber shows very little mould colonisation, tending to be colonised by sapstain fungi. It appears, therefore that the physiological state of the wood may influence the pattern of colonisation. A laboratory trial was underta...
J R Williams, D J Dickinson, J F Webber


Integrated protection of freshly sawn lumber using Bacillus subtilis and selected fungicide
1997 - IRG/WP 97-10235
Bioprotection against stain fungi has tremendous potential for reducing discoloration of freshly sawn wood while decreasing chemical consumption. Unfortunately, most bioprotectants appear to be unable to consistently perform under the array of conditions to which freshly sawn wood is exposed. While research is underway to understand the nature of the inconsistent performance, a more pragmatic appr...
M E Mankowski, M Anderson, J J Morrell


Chelator production and cellobiose dehydrogenase activity of wood inhabiting fungi
2000 - IRG/WP 00-10363
Multiple components have been postulated to be involved in the Fenton-based biological degradation of wood by brown rot fungi. Chelators have been implicated in essential iron reduction steps. Cellobiose dehydrogenase activity and quinone reducing activity have also been implicated in brown rot biodegradation. When biochelator production by three white rot fungi, three brown rot fungi and three wo...
Weihong Qi, J Jellison


Biological control of decay
1975 - IRG/WP 135
One approach to the biological control of decay in standing poles, live trees or seedlings is with microbial immunising commensals or IC.·These are microorganisms able to grow in the wood without damaging it and as a result of such growth to protect against certain types of decay. Scytalidium and Trichoderma spp have been tested in the field. The latter species have shown an establishment rate in...
J Ricard


Effect of nutrient regimes, temperature, pH, and wood sterilization method on performance of selected bioprotectants against wood staining fungi
1992 - IRG/WP 92-1551
The effect of nutrient regimes, incubation temperature, media pH, and wood sterilization method on performance of four potential bioprotectants (Pseudomonas putida, Pseudomonas cepacia, Bacillus subtilis, and Trichoderma harzianum) against wood staining fungi were evaluated using small ponderosa pine samples over a four week period. Incubation at 32°C resulted in slight increases in the degree of...
J J Morrell, C M Secton


Preliminary evaluations of a small wafer assay for screening potential biological control agents
1989 - IRG/WP 2332
Screening potential biological agents for controlling wood decay fungi poses a dilemma. The ideal test would eliminate as many variables as possible. Most tests utilize pure cultures of the test organism on an artificial media which in no way resembles wood. The use of sawdust improves this approach, but the sawdust increases fungal access to the lignocellulose matrix, potentially inflating the im...
C M Freitag, J J Morrell


A comparative study of some anti-splitting devices, using Eucalyptus saligna and hybrids of Eucalyptus saligna X Eucalyptus spp poles
1983 - IRG/WP 3251
It is commom for some Brazilian wood pole users to require devices such as banding steel, to avoid or reduce splits at the end of Eucalyptus treated poles. On other hand users and manufacturers do not have data to confirm the effectiveness of the banding steel types normally used such as galvanized "Steel-Belt" and galvanized "Wire-Belt". For this reason a comparative study was carried out includi...
F C Geraldo, J A C Sodré


Biological control of wood decay
1992 - IRG/WP 92-1531
This paper provides a general discussion of the principles underlying the concept of using biological control agents for the protection of wooden materials from decay. The rationale for the current upsurge in interest in biological control is discussed and a critical appraisal of the technology compared with the use of chemical wood preservatives is included. The advantages and limitations associa...
A Bruce


Variable tolerance of Ophiostoma spp. and Diplodia pinea to commercial antisapstain products
1998 - IRG/WP 98-10266
A recent survey of the occurrence of sapstain fungi in New Zealand, conducted at The University of Waikato, provided the opportunity to examine fungicidal tolerance amongst new isolates. It also enabled a comparison of tolerance amongst new isolates with those used in routine antisapstain screening trials at Forest Research. A rapid antisapstain laboratory disc trial was used to determine the ext...
D R Eden, C M Chittenden, B Kreber, J G Van der Waals, R N Wakeling, R L Farrell, T Harrington


Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti


Effect of thickened boron in preventing conidial germination of sapwood-inhabiting fungi
1994 - IRG/WP 94-30059
We evaluated the efficacy of thickened boron preservative "Diffusolä" in preventing conidia germination of sapwood-inhabiting fungi using plate bioassay, Southern Yellow Pine and sweetgum block tests, and green pine log sections. The test fungi were sapstain fungi Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans and mold fungi, Aspergillus niger, Penicillium spp, and Tric...
S C Croan


Natural durability of larch heartwood against decay
1997 - IRG/WP 97-10201
Larch heartwood has been promoted in Sweden and in other European countries as an alternative to preservative treated timber. It has even beeen stated that larch heartwood is more durable than preservative treated timber. This paper presents a compilation of facts from scientific reports on the durability of larch heartwood. Some recent data from this department are also reported. All data suggest...
T Nilsson


Application of DNA fingerprinting methods to identify biocontrol strains of fungi imperfecti
1994 - IRG/WP 94-10068
We have analyzed a number of biocontrol strains of Trichoderma harzianum and other Trichoderma strains with the methods DNA fingerprinting and PCR fingerprinting to differentiate and identify these strain which is not possible with morphological or biochemical methods. We could differentiate even gamma-ray induced mutants from each other as well as different strains form the same and different spe...
A Schlick, K Kuhls, W Meyer, E Lieckfeld, T Börner, K Messner


Molecular phylogeny of Ophiostoma spp. related to blue-stain in pine
2002 - IRG/WP 02-10432
Blue-stain in coniferous woods is an economically important disease in many temperate countries, caused specially by genus Ophiostoma. Classical taxonomy of genus Ophiostoma is limited for the correct identification of many species, because of the similar macroscopic and microscopic characters, as well as the existence of some species complex. Molecular identification is a good complement to eluci...
M Villarreal, F Arenal, J Sánchez-Ballesteros, M T De Troya, F Llinares, V Rubio, A Navarrete


Biological control of wood decay fungi. - Part II. Effects of exogenous nitrogen on effectiveness
2000 - IRG/WP 00-10360
The effects of nitrogen (urea and ammonium nitrate) on the ability of Trichoderma harzianum to inhibit the enzyme capabilities and decay capacities of Trametes versicolor and Gloeophyllum trabeum were investigated using a small wood sandwich decay test. The biocontrol fungus generally had a greater effect on the brown rot fungus, confirming previous reports. Nitrogen produced inconsistent effects ...
E A Canessa, J J Morrell


Effect of substrate type and moisture requirements in relation to colony initiation in two carpenter ant species
1999 - IRG/WP 99-10320
Conditions necessary for optimal colony initiation or the rate of initial colony expansion by early brood in the carpenter ant species Camponotus modoc and C. vicinus on various substrates conditioned to different moisture contents were studied. Camponotus modoc and Camponotus vicinus queens were placed in Douglas-fir, western red cedar and Styrofoam® blocks conditioned in sealed chambers at 70% ...
M E Mankowski, J J Morrell


Enzymatic study of Ceratocystis sp., blue-stain fungi on Pinus nigra
1999 - IRG/WP 99-10315
One of the main problems that the forest exploitation industry has with Pinus nigra wood is the blue-stain fungi, whose causing agent is unknown. Therefore, the objective of this work has been to study, through enzymatic tests of the isolated cultures, if these fungi infect Pinus nigra in any specific way. After the incubations, isolates of Ceratocystis were obtained. These were cultured in a sali...
M T De Troya, F Rubio, D Muñoz-Mingarro, F Llinares, C Rodríguez-Borrajo, M Yuste, M J Pozuelo, J I Fernández-Golfín


Microdistribution of Copper in Copper-Ethanolamine (Cu-EA) Treated Southern Yellow Pine (Pinus spp.) related to density distribution
2004 - IRG/WP 04-40270
The relationship between copper absorption and density distribution in wood cell walls was investigated in this study. The density distribution on layer level was obtained from two approaches: (1) calculation by using data obtained from literature; (2) microdistribution of carbon and oxygen atoms in the wood cell. The microdistribution of carbon and oxygen in untreated southern yellow pine (Pinus ...
Jinzhen Cao, D P Kamdem, E Pasek


Effects of chemical pretreatment of Douglas-fir heartwood on efficacy of potential bioprotection agents
1990 - IRG/WP 1440
Biological protection against wood decay fungi represents an environmentally attractive alternative to the use of chemicals; however, the process of identifying suitable candidates and the conditions that ensure successful protection pose major challenges. One critical aspect of this problem is to identify organisms that can rapidly and uniformly colonize the wood. Wood contains low levels of nutr...
B Dawson-Andoh, J J Morrell


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce


The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell


Protection for whole-of-house timbers from subterranean termites in Australia
2005 - IRG/WP 05-20315
A field study was designed to increase maximum protection of whole-of-house timbers against subterranean termites. Concrete slab and suspended floor simulated houses were constructed using untreated and treated (slow acting toxicants) timber frames. The treated and untreated timber structures within the simulated houses were exposed with or without soil chemical and/or physical barriers in the fie...
B M Ahmed, J R J French, P Vinden, P Blackwell, J Hann


Biological control of internal decay in Scots pine poles: A seven year experiment
1992 - IRG/WP 92-1529
The interactions between key fungi were monitored, by non-destructive and destructive sampling methods, during the course of an experiment on the effectiveness of Scytalidium sp. and Trichoderma spp. for eliminating established infections of Lentinus lepideus (Fr. ex Fr.) Fr. in creosoted Pinus sylvestris poles. Although extensively isolated at the beginning and end of the experiment, Trichoderma ...
P I Morris, D J Dickinson, B Calver


Preliminary agar screening studies as the first stage in the development of a biological control system against basidiomycetes in ground contact situations
1995 - IRG/WP 95-10111
Preliminary screening of Trichoderma isolates as possible biocontrol agents for protection of wood in ground contact situations against basidiomycete decay was undertaken on three types of media. Media used were 3% malt extract agar (MEA); a low nutrient synthetic media with a carbon: nitrogen ratio typical of that found in softwoods (LNM); and a similar synthetic media where the amino acid profil...
E J B Tucker, A Bruce


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