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Fenton's reagent as a modification tool in brown-rot decay
1996 - IRG/WP 96-10155
A biomimetic approach was used to clarify the role and importance of the Fenton-type reaction in the carbohydrate degradation by brown-rot fungi. Spruce sawdust and microcrystalline cellulose were modified in the H2O2/Fe(II) treatment. The degree of hydrolysis of the pretreated spruce sawdust was clearly increased with the complete cellulase (Econase), purified endoglucanase from Trichoderma reese...
M Rättö, A-C Ritschkoff, J Buchert, L Viikari


Laboratory screening to determine the preventive effectiveness against blue stain fungi and moulds
1991 - IRG/WP 3677
This paper reports results of preservative treatment and leaching experiments, using borax, polybor and boracol 20, on small wood blocks of English oak and American pitch pine heartwood. Earlier experiments on the performance of various biocides as possible additives to bilgewater to prevent fungal decay of shipping timbers had suggested that some formulations of boron might be associated with phy...
M T De Troya, A M Navarrete


Protection of wood blocks treated with Trichoderma isolates selected on the basis of preliminary agar screening studies
1996 - IRG/WP 96-10154
Previously reported results of agar interaction screening studies for biocontrol agents of wood decay basidiomycetes showed two Trichoderma viride isolates, killed 16 of 19 target fungi (Tucker and Bruce, 1995). Testing of these isolates in wood was required to assess their performance at preventing decay of wood blocks. Standard testing of chemical wood preservatives is used to determine the toxi...
E J B Tucker, A Bruce, H J Staines


Natural resistance of Bamboo (Bambusa sp.) to marine wood-borers in Goa waters (India)
1993 - IRG/WP 93-10032
The paper deals with the natural durability of Bambusa sp. against the attack of marine wood-borers in Goa waters. Test specimens of this species were completely destroyed within a short period of nine months due to severe attack of borers indicating its very low natural resistance. Wood-borers involved were Martesia striata (Linnaeus), Nausitora hedleyi Schepman and Lyrodus pedicellatus (Quatrefa...
L N Santhakumaran, S G Sawant


Susceptibility of Lentinus lepideus (Fr.:Fr.) Fr. to volatiles produced by Trichoderma spp
1987 - IRG/WP 1316
A range of Trichoderma species and strains were tested for their ability to produce fungistatic volatiles. Volatiles from all isolates produced fungistatic effects on Lentinus lepideus however wide interspecies and interstrain variability in the level of response was evident. Cross plating studies between the Trichoderma isolates and Lentinus lepideus showed that Lentinus lepideus was overgrown an...
A Bruce, C Johnstone, J A P McVey


Natural durability of 4 different Larix species tested in soil contact
2002 - IRG/WP 02-10434
Importers of Siberian Larch claimed to have a material which can replace pressure treated wood in soil contact. This gave reason to investigate the durability of 4 different Larix species (L. decidua, L. sibirica, L. decidua x sibirica, L. gmelini var ologenis) coming from 7 different origins in comparison with sapwood of Pinus sylvestris untreated as well as pressure impregnated with retentions o...
A O Rapp, H Viitanen, T Nilsson


Antifungal properties of metabolites produced by Trichoderma isolates from sawdust media of edible fungi against wood decay fungi
1994 - IRG/WP 94-10051
Trichoderma isolated from a sawdust medium of Pholiota nameko produced filtrates which had antifungal activities to four wood decay fungi tested on the agar plates. Filtrates produced from another isolate, from a sawdust medium of Lentinus edodes, had antifungal activities only to the white rot fungi, Coriolus versicolor and Pycnoporus coccineus. These results did not agree with those from earlier...
S Doi, M Mori


Laboratory evaluation of potential antisapstain treatments for Pinus radiata
1983 - IRG/WP 3237
Twelve formulations were tested in the laboratory using a 'mini' board test for effectiveness on Pinus radiata (D. Don) against sapstain, mould and decay fungi. Three formulations, sodium pentachlorophenoxide (NaPCP) plus borax, Haipen 50 WP, and Mitrol 375, are used commercially in New Zealand. Busan 1009 (0.5% product), IWD Product A (0.2% ai), IWD Product B (0.3% ai), Fennotox...
J A Drysdale


The effect of precolonisation of blocks with common pole resident fungi on subsequent biological control of Lentinus lepideus by Trichoderma spp
1989 - IRG/WP 1387
Results of previous field studies have indicated that the spread of Trichoderma throughout the groundline regions of the interiors of creosoted poles are adversely influenced by the presence of large populations of resident mould organisms. Failure of the control fungus to develop and colonize all of the decay susceptible groundline region means that sucessful control of decay is most unlikely. Th...
A Bruce, T L Highley


The effect of Trichoderma volatiles on the growth and enzyme production of Serpula lacrymans
2000 - IRG/WP 00-10332
Although various authors have reported the biological control of Serpula lacrymans by Trichoderma spp. the mode of antagonism employed by Trichoderma is not yet clear. The work presented here concentrates on the production of anti-fungal volatiles for inhibition of S. lacrymans growth. Volatile mediated interactions were examined between four S. lacrymans isolates and a range of nine known Trichod...
S N Humphris, A Bruce, R E Wheatley


Disodium octaborate tetrahydrate, alone and in conjunction with a selected isolate of Trichoderma viride, reduces decay of fresh felled pine independent of the effect of weathering
1994 - IRG/WP 94-10054
Unpeeled billets of Corsican pine were treated with the following: 5% aqueous disodium octaborate tetrahydrate (DOT) solution; 5% DOT solution with an isolate of Trichoderma viride with a low susceptibility to borate; the Trichoderma viride isolate alone. Half of the billets in each treatment group were also placed in large-diameter plastic pipes to protect the billets from the weather. All three ...
M W Schoeman, D J Dickinson, J F Webber


Assessment of the Inhibition of wood decay fungi by volatile organic compounds identified from Trichoderma spp.
1999 - IRG/WP 99-10302
Previous research identified five volatile organic compounds produced by Trichoderma spp. that may be inhibitory to wood decay fungi. The effects of four of these volatile organic compounds, 2-propanone, 2-methyl-1-butanol, heptanal and octanal were tested over a range of concentrations against four selected wood decay fungi. The fungi were incubated in malt extract broth under appropriate conditi...
S N Humphris, R E Wheatley, A Bruce, C Payne


Laboratory tests on the natural durability of timber methods and problems
1984 - IRG/WP 2217
In literature a large variety of test methods is mentioned to examine the natural resistance of timber against fungal attack. This concerns the kind of sampling as well as the test procedure, the test fungi, the duration of test, and the classification of the resistance according to the test results. These variations, however, are of great influence on the test result. Long term exposure will lead...
H Willeitner


Chelator production and cellobiose dehydrogenase activity of wood inhabiting fungi
2000 - IRG/WP 00-10363
Multiple components have been postulated to be involved in the Fenton-based biological degradation of wood by brown rot fungi. Chelators have been implicated in essential iron reduction steps. Cellobiose dehydrogenase activity and quinone reducing activity have also been implicated in brown rot biodegradation. When biochelator production by three white rot fungi, three brown rot fungi and three wo...
Weihong Qi, J Jellison


Siderophore production by Trichoderma spp. and its importance in the biological control of wood decay fungi
1994 - IRG/WP 94-10070
Competition for iron as well as other micro-nutrients is an essential component of the microbial ecology of many ecosystems. A wide range of micro-organisms including fungi and bacteria have been shown to increase their ability to efficiently capture iron through the production of specialised iron chelating compounds called siderophores. Since iron is in low supply in wood and has been implicated ...
U Srinivasan, A Bruce, T L Highley


Biological control of decay
1975 - IRG/WP 135
One approach to the biological control of decay in standing poles, live trees or seedlings is with microbial immunising commensals or IC.·These are microorganisms able to grow in the wood without damaging it and as a result of such growth to protect against certain types of decay. Scytalidium and Trichoderma spp have been tested in the field. The latter species have shown an establishment rate in...
J Ricard


Study of the degradation caused by micro-organisms in Pinus sp. waterlogged wood
1989 - IRG/WP 1411
So far, the different Centers are trying the restoration and the conservation of wood structures, coming from subaquatic archeological deposits, with interest from the historic - artistic point of view. The main objective of this paper has been the determination of the decay level of Pinus sp. wood coming from a roman ship (approximately 2000 years old), where we have analyzed their physical prope...
M T De Troya, M C Escorial, J Garcia, A Cabanas


Effect of Volatiles from Trichoderma species on the regulation of protein synthesis in Serpula lacrymans isolates
2002 - IRG/WP 02-10440
The growth of wood decay fungi has previously been shown to be inhibited by volatile organic compounds (VOCs) from selected Trichoderma isolates. No mechanism of action has however been established for such inhibition. This paper reports the effects of VOCs produced by Trichoderma pseudokoningii, T. viride and T. aureoviride on the growth of four dry rot isolates and corresponding protein synthesi...
S Humphris, R Wheatley, E Buultjens, A Bruce


Effect of nutrient regimes, temperature, pH, and wood sterilization method on performance of selected bioprotectants against wood staining fungi
1992 - IRG/WP 92-1551
The effect of nutrient regimes, incubation temperature, media pH, and wood sterilization method on performance of four potential bioprotectants (Pseudomonas putida, Pseudomonas cepacia, Bacillus subtilis, and Trichoderma harzianum) against wood staining fungi were evaluated using small ponderosa pine samples over a four week period. Incubation at 32°C resulted in slight increases in the degree of...
J J Morrell, C M Secton


Preliminary evaluations of a small wafer assay for screening potential biological control agents
1989 - IRG/WP 2332
Screening potential biological agents for controlling wood decay fungi poses a dilemma. The ideal test would eliminate as many variables as possible. Most tests utilize pure cultures of the test organism on an artificial media which in no way resembles wood. The use of sawdust improves this approach, but the sawdust increases fungal access to the lignocellulose matrix, potentially inflating the im...
C M Freitag, J J Morrell


Intraspecific variability of durability of Wapa courbaril (Eperua grandiflora) against Antrodia sp. and Coriolus versicolor: effect of radial and height position in the stem
2004 - IRG/WP 04-10531
The variation of a lesser-used species, Eperua grandiflora attacked by brown and white rot has been examined. Trees of this specie showed differences in their behaviour against the type of rot used and also showed evidence of intra and inter tree variation. The “tree” effect is very significant concerning natural resistance. In the same way, there is variation of durability observed according ...
N Amusant, J Beauchêne, M Fournier


Improved preservative penetration of spruce after pre-treatment with selected fungi. II. Creosote treatment, analysis and strength testing
1998 - IRG/WP 98-40106
This paper describes the creosote treatment and analysis of logs pre-treated with selected fungal agents as a method of increasing the porosity of the wood prior to preservative treatment. The paper also reports the subsequent strength testing of the timber to evaluate the effects of the fungal pre-treatment on the structural integrity of the wood. European spruce logs were pre-treated with eithe...
E J Tucker, A Bruce, H J Staines, B Rosner, K Messner


Biological control of wood decay
1992 - IRG/WP 92-1531
This paper provides a general discussion of the principles underlying the concept of using biological control agents for the protection of wooden materials from decay. The rationale for the current upsurge in interest in biological control is discussed and a critical appraisal of the technology compared with the use of chemical wood preservatives is included. The advantages and limitations associa...
A Bruce


Improved preservative penetration of spruce after pre-treatment with selected fungi. I. Fungal pre-treatment of pole sections
1998 - IRG/WP 98-40117
European spruce logs were pre-treated with either Phanaerochete chrysosporium, Dichomitus squalens, Trichoderma viride and Trichoderma aureoviride for incubation periods of between 1-4 weeks prior to pressure treatment with creosote in a commercial treatment plant and analysed for strength and creosote uptake (see part II Tucker et al.). A method to pre-treat logs was optimised. The pole sections ...
B Rosner, K Messner, E J B Tucker, A Bruce


Antagonistic effect of Trichoderma spp. against basidiospores
1993 - IRG/WP 93-10027
All screening of potential biocontrol agents of wood decay fungi have previously been used against basidiomycete mycelium. Similarly, experiments designed to evaluate the mechanisms involved in antagonism (soluble metabolites and volatile antibiotics) between biological control agents and target fungi have always been carried out on mycelial inoculum. Basidiospores are a primary source of infectio...
U Srinivasan, T L Highley, S C Croan, A Bruce


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